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Frequency of IL-10-producing regulatory B cells associated with disease activity in thyroid-associated orbitopathy

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International Journal of Ophthalmology(English edition) 2018年第9期11卷 1458-1462页

作者： Yun-Gang Ding Guo Chen Qian Li Xiao-Feng Wen Lai Wei hua-sheng yang State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina Qindao Ludong Eye Hospital Qingdao 266600Shandong ProvinceChina Ningxia Eye Hospital People's Hospital of Ningxia Hui Autonomous Regionthe First Clinical College of Northwest University for Nationalitiesthe Cooperative Teaching Hospital of North Minzu UniversityYinchuan 750001Ningxia Hui Autonomous RegionChina

AIM： To investigate the association between IL-10-producing regulatory B（B10） cells and the clinical features of thyroid-associated orbitopathy（TAO）. METHODS： A total of 30 patients with TAO were recruited at Zhongshan Ophthalmic Center from May 2015 to December 2015. Peripheral blood mononuclear cells（PBMCs） were separated from blood samples of 30 TAO patients and 16 healthy controls and stimulated with CD40 ligand and CpG for 48h. The frequency of IL-10＋ B cells was examined by flow cytometry and the correlation between the frequency of IL-10＋ B cells and clinical features of TAO was analyzed by SPSS. RESULTS： The frequency of IL-10＋ B cells among CD19＋ B cells in TAO patients was significantly lower than in healthy controls（TAO： 4.66%±1.88% vs healthy control： 6.82%±2.40%, P〈0.01）. The frequency of IL-10＋ B cells showed a positive correlation with disease activity of TAO measured by Clinical Activity Score（CAS）（r=0.50, P〈0.01）, and became higher in TAO patients with family history of Graves＇ disease（GD）（P=0.04）. CONCLUSION： The decrease of the frequency of IL-10＋ B cells in TAO patients indicates the deficiency of B10 cells in TAO, and the positive association with disease activity suggests its important role in TAO inflammation regulation.

关键词： thyroid-associated orbitopathy regulatory B cells interleukin-10 flow cytometry

Immunohistochemical features of carcinoma ex pleomorphic adenoma and pleomorphic adenoma in the lacrimal gland

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International Journal of Ophthalmology(English edition) 2019年第8期12卷 1238-1242页

作者： Ping Zhang Li-Juan Tang huan-huan Gao Wen-Xin Zhang Jian-Xian Lin hua-sheng yang Department of Ocular Pathology State Key Laboratory ofOphthalmologyZhongshan Ophthalmic CenterSun Yat-senUniversityGuangzhou510060Guangdong ProvinceChina Department of Orbital Disease and Oncology State KeyLaboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou510060GuangdongProvinceChina

AIM: To investigate C-myc, Ki-67, pan-cytokeratin, and vimentin immunohistochemical features of carcinoma ex pleomorphic adenoma(Ca-ex-PA) and pleomorphic adenoma(PA) in the lacrimal gland in order to find some clues in the differential diagnosis between ***: We reviewed microscopic slides and clinical records of 64 cases of PA and 15 cases of Ca-ex-PA in the lacrimal gland. Immunohistochemical antibodies for C-myc, Ki-67, pan-cytokeratin, and vimentin were ***: Median age of PA was 43.2 y(from 21 to 75). The 35 patients(54.7%) were male and 29 patients(45.3%) were female. For the PAs, the average positivity of C-myc was 4.6%;the average proliferation index of Ki-67 was 3.2%;pan-cytokeratin was positive in ductal cells, and vimentin was positive in myoepithelial cells. Median age of Ca-ex-PA was 54.3 y(from 26 to 76). There were 7 male patients(46.7%) and 8 female patients(53.3%). Among 15 Ca-ex-PAs, there were 6 myoepithelial carcinomas, 4 adenocarcinomas, 3 epithelial-myoepithelial carcinomas, and 2 squamous cell carcinomas. For the Ca-ex-PAs, the average positivity of C-myc was 36.4%;the average proliferation index of Ki-67 was 29.2%;pan-cytokeratin was positive in all cases, and vimentin was positive in myoepithelial ***: PA has a lower positivity of C-myc and Ki-67, while Ca-ex-PA had a higher positivity of these two biomarkers. These four biomarkers as a set could provide valuable clues in the differential diagnosis between Ca-exPA and PA. Our results indicate that the activation of C-myc could play an important role in the pathogenesis of Ca-exPA and PA.

关键词： carcinoma ex pleomorphic adenoma pleomorphic adenoma C-myc immunohistochemistry

Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation

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International Journal of Ophthalmology(English edition) 2018年第9期11卷 1463-1466页

作者： Li-Juan Tang Li-Jun Zhou Wen-Xin Zhang Jian-Yan Lin Yong-Ping Li hua-sheng yang Ping Zhang State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen University

AIM： To reveal the expression of multidrug-resistance associated proteins： glutathione-S-transferase π（GSTπ）, P-glycoprotein（P-gp） and vault protein lung resistance protein（LRP） in retinoblastoma（RB） without any conservative treatment before primary enucleation and to correlate this expression with histopathological tumor features. METHODS： A total of 42 specimens of RB undergone primary enucleation were selected for the research. Sections from the formalin-fixed, paraffin-embedded specimens were stained with HE and immunohistochemistry to detect the expression of GSTπ, P-gp and ***： GSTπ was expressed in 39/42（92.86%） RBs and in 9/9（100%） well-differentiated RBs. P-gp/GSTπ was found in 30（71.42%） of 42 RBs. Totally 9（21.43%） tumors were well differentiated and 33（78.57%） were poorly differentiated. Totally 15（35.71%） eyes had optic nerve（ON） tumor invasion, 36（85.71%） had choroidal tumor invasion, and 14（33.33%） had simultaneous choroidal and ON invasion. There was no statistically significant relationship between P-gp, GSTπ, LRP positivity and the degree of ocular layer tumor invasion and ON tumor invasion（P〉0.05）. CONCLUSION： RB intrinsically expresses GSTπ, P-gp and LRP. GSTπ expression is positive in 100% welldifferentiation ones, so in which way it is correlated with differentiation. But the other two proteins expressions are not related to tumor differentiation and to the degree of tumor invasion. GSTπ may be a new target of chemotherapy in RB.

关键词： glutathione-S-transferase π P-glycoprotein vault protein lung resistance protein retinoblastoma multidrug-resistance proteins

Relationship of lipid and lipoprotein ratios with coronary severity in patients with new on-set coronary artery disease complicated with type 2 diabetics

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Journal of Geriatric Cardiology 2016年第8期13卷 685-692页

作者： Ying DU Juan CHEN Man-hua CHEN sheng-hua yang Sha LI Yuan-Lin GUO Cheng-Gang ZHU Rui-Xia XU Qian DONG Jian-Jun LI Division of Dyslipidemia State Key Laboratory of Cardiovascular Disease Fu Wai Hospital National Center for Cardiovascular Diseases Chinese Academyof Medical Sciences and Peking Union Medical College Beijing China Department of Cardiology the Central Hospital of Wuhan Wuhan Hubei China

Background Diabetes mellitus （DM） is associated with coronary artery disease （CAD） progression. Although previous studies have demonstrated the association of lipid and lipoprotein ratios with CAD, no data are currently available concerning the relationship between lipid and lipoprotein ratios and the severity of new on-set CAD in diabetics. Therefore, the aim of the present study was to investigate the usefulness of lipid and lipoprotein ratios in predicting the severity of CAD in patients with type 2 DM （T2DM）. Methods A total of 380 consecutive T2DM patients with new on-set CAD were enrolled in the present study. Then, they were classified into the three groups ac-cording to Gensini score （GS） tertiles. The relationship between lipid and lipoprotein ratios currently used and the GS was investigated. Results Positive correlations of natural log-transformed GS （lnGS） with apolipoprotein B to apoA-I ratio （apoB/apoA-I）, non-high-density lipoprotein cholesterol to apoA-I ratio （non-HDL-C/apoA-I）, and low-density lipoprotein cholesterol to apoA-I ratio （LDL-C/apoA-I） were found （r = 0.18, 0.13, 0.12, respectively, all P 〈 0.05）. Multivariate logistic analysis indicated apoB/apoA-I as the strongest predictor for high GS （OR = 5.67, 95% CI： 1.45-23.92, P = 0.003）. Area under receivers operating characteristic curve of apoB/apoA-I was 0.63 （95% CI： 0.60-0.66, P = 0.001） for predicting high GS. The optimal cutoff value of apoB/apoA-I to predict high GS was 0.72 with the sensitivity of 61.2% and the specificity of 62.1%. Conclusions Lipid and lipoprotein ratios might be useful for predicting the severity of new on-set CAD in T2DM patients, and the apoB/apoA-I appeared as the most significant predictor in this population.

关键词： Coronary artery disease Lipid Lipoprotein Type 2 diabetes mellitus

Early liver retransplantation versus late liver retransplantation： analysis of a single-center experience

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Chinese Medical Journal 2008年第20期121卷 1992-1996页

作者： CHEN Gui-hua FU Bin-sheng yang yang CAI Chang-jie LU Min-qiang LI hua WANG Gen-shu YI Shu-hong XU Chi ZHANG Jun-feng ZHANG Tong WANG Guo-ying Liver Transplantation Center Third Affiliated Hospital of Sun Yat-sen University Transplantation Research Institute of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background Orthotopic liver retransplantation （re-OLT） is the only effective therapy for irreversible failure of a liver graft. Early and late graft failure gives way to two different clinical conditions that should be discussed separately. This study was designed to compare early and late re-OLT for patients with poor graft function after primary transplantation at our center and sum up our clinical experience in re-OLT. Methods The clinical data of 31 re-OLTs at our center from January 2004 to February 2007 were analyzed retrospectively, consisting of the first group with 14 cases of early re-OLT and the second group with 17 cases of late re-OLT.Results Biliary tract complications were the main indications for early re-OLT （57.1%） and late re-OLT （52.9%）. Other common indications were vascular complications in early re-OLT and recurrence of primary diseases in late re-OLT. No significant differences were found between the groups with regard to the volume of bleeding during operation, cold ischemia time, operative duration, and perioperative mortality; except for the model of end-stage liver disease （MELD） score. Outcome was fatal for 7 patients in early re-OLT and 9 patients in late re-OLT. Two deaths were due to multiple organ failure with 3 deaths due to severe sepsis-related disease in early re-OLT, and 4 deaths were due to severe sepsis-related disease with 3 deaths due to recurrence of hepatocellular carcinoma （HCC） in late re-OLT. One and 2-year actuarial survival rates after re-OLT were 55.2% and 36.9%, respectively, for patients in early re-OLT, and 65.1% and 52% respectively, for patients in late re-OLT. No significant differences were found regarding survival rates between the two groups. Conclusions Similar clinical results can be achieved in early and late re-OLT. Proper indications and optimal operation timing, adequate preoperative preparation, experienced surgical procedures, and effective perioperative anti-infection strategy contribute to th

关键词： liver transplantation retransplantation survival rate

视网膜母细胞瘤组织中HPV-DNA表达的研究

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国际眼科杂志 2013年第11期13卷 2185-2187页

作者：刘国颖杨华胜南方医科大学附属南海医院眼科中国广东省佛山市528200 中国广东省广州市中山眼科中心眼眶病与眼肿瘤科 510080

目的:研究我国视网膜母细胞瘤(retinoblastoma,RB)中HPV-DNA表达情况,为进一步揭示HPV在散发性RB中作用提供初步依据。方法:收集22例散发性RB患者新鲜肿瘤组织标本,应用PCR方法及引物MY09/11检测肿瘤组织中HPV-DNA表达情况。结果:选取22... 详细信息

目的:研究我国视网膜母细胞瘤(retinoblastoma,RB)中HPV-DNA表达情况,为进一步揭示HPV在散发性RB中作用提供初步依据。方法:收集22例散发性RB患者新鲜肿瘤组织标本,应用PCR方法及引物MY09/11检测肿瘤组织中HPV-DNA表达情况。结果:选取22例RB肿瘤标本中7例HPV-DNA阳性(32%),均为单眼散发患者(包括男4例,女3例),2例双眼散发患者均为阴性。结论:HPV可能是我国散发性RB发病因素之一,但其明确作用及机制尚待进一步研究。

关键词：视网膜母细胞瘤人乳头瘤病毒发病因素

Enhancing postoperative pain control by surgically-initiated rectus sheath block in abdominal aortic aneurysm open repair: A case report

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World Journal of Clinical Cases 2025年第6期13卷 41-47页

作者： Kuan-hua Chen Ming-Yuan Kang Yi-Ting Chang sheng-yang huang Yung-Szu Wu Center for Cardiovascular Disease Taichung Veterans General HospitalTaichung 407219TaiwanChina Department of Anesthesiology Taichung Veterans General HospitalTaichung 407219TaiwanChina Division of Pediatric Surgery Department of SurgeryTaichung Veterans General HospitalTaichung 407219TaiwanChina School of Medicine National Yang Ming Chiao Tung UniversityTaipei 112304TaiwanChina Department of Post-Baccalaureate Medicine National Chung Hsing UniversityTaichung 402202TaiwanChina

BACKGROUND Abdominal aortic aneurysm(AAA)repair often involves significant postoperative pain,traditionally managed with systemic opioids,which can cause undesirable side *** case report explores the novel use of a surgically-initiated rectus sheath block with a catheter-over-needle assembly for pain management in AAA *** SUMMARY A 67-year-old female with hypertension and previous aortic dissection underwent elective open repair of an infrarenal AAA,which had grown from 3.4 cm to 4.3 cm over 14 months.A rectus sheath block was initiated surgically for postoperative pain *** patient reported low pain scores and did not require systemic intravenous opioids,enabling early ambulation and discharge on postoperative day seven without *** preventing complications of systemic opi-oids,the method indicating a promising direction for postoperative pain management in major vascular *** Surgically-initiated rectus sheath block as a valuable tool for managing postoperative pain in AAA repair.

关键词： Abdominal aortic aneurysm Postoperative pain management Rectus sheath block Surgical anesthesia Opioid-sparing techniques Case report

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甲状腺相关眼病的非手术治疗现状及研究进展

国际眼科杂志 2022年第8期22卷 1288-1292页

作者：王星叶慧菁杨华胜中山大学中山眼科中心眼科学国家重点实验室广东省眼科视觉科学重点实验室广东省广州市510060

甲状腺相关眼病(thyroid-associated ophthalmopathy, TAO)是一种与甲状腺功能异常密切相关的自身免疫性疾病,是成人最常见的眼眶疾病之一。临床表现复杂多样,病情进展可引起眼球突出、复视,暴露性角膜炎、角膜溃疡和压迫性视神经病变,... 详细信息

甲状腺相关眼病(thyroid-associated ophthalmopathy, TAO)是一种与甲状腺功能异常密切相关的自身免疫性疾病,是成人最常见的眼眶疾病之一。临床表现复杂多样,病情进展可引起眼球突出、复视,暴露性角膜炎、角膜溃疡和压迫性视神经病变,导致不可逆性视力损害甚至失明。治疗上缺乏特异性,仅以临床对症治疗为主,疗效不确定,目前该病是眼科的难点。临床一线药物治疗以糖皮质激素为主,二线治疗如免疫抑制剂、放射治疗等,均存在一定局限性。随着对疾病认识的深入、全面,根据TAO发病机制研发出了以替妥木单抗、利妥昔单抗为代表的多种新型靶向药物,展现出缓解炎症、控制病情的优良作用,是未来的研究方向。本文就TAO的非手术治疗现状及研究进展做一简要综述。

关键词：甲状腺相关眼病非手术治疗糖皮质激素麦考酚酸酯替妥木单抗

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li Hui-Min Xing Hai-Dong Qian Qiao Gao sheng-Lan Xu hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles