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不同黄颡鱼家系组注射嗜水气单胞菌后免疫相关指标的比较研究

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淡水渔业 2017年第5期47卷 40-46页

作者：秦钦王明华陈校辉蒋广震张世勇钟立强潘建林刘文斌江苏省淡水水产研究所南京210017 南京农业大学无锡渔业学院江苏无锡214081 南京农业大学动物科技学院江苏省水产动物营养重点实验室南京210095

为了探究不同黄颡鱼(Pelteobagrus fulvidraco)家系组的免疫性能,选取Ⅰ(G♂×G♀)、Ⅱ(S♂×S♀)、Ⅲ(T♂×T♀),Ⅳ(G♂×S♀)和V(S♂×T♀)5个家系组130日龄的鱼种300尾/家系,试验鱼体重为(8.5±1.0)g,开展注射嗜水气单胞菌攻毒试验... 详细信息

为了探究不同黄颡鱼(Pelteobagrus fulvidraco)家系组的免疫性能,选取Ⅰ(G♂×G♀)、Ⅱ(S♂×S♀)、Ⅲ(T♂×T♀),Ⅳ(G♂×S♀)和V(S♂×T♀)5个家系组130日龄的鱼种300尾/家系,试验鱼体重为(8.5±1.0)g,开展注射嗜水气单胞菌攻毒试验。在攻毒0 h及攻毒后6、24、48 h采样。测定累积死亡率(CM)及血浆、肝脏免疫相关指标,包括血浆总蛋白(TP)、乳酸(LA)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(AKP);肝脏超氧化物歧化酶(SOD)、丙二醛(MDA)。结果显示:各家系组间黄颡鱼种感染嗜水气单胞菌后,除血浆AKP之外的指标都表现出组间显著差异。攻毒48 h后,CM由高到低顺序为Ⅱ>V>Ⅲ>Ⅳ>I。攻毒24 h后血浆TP达到高峰然后逐步下降。在攻毒48 h后,家系组Ⅰ、Ⅳ保持了较高TP含量,与其他试验组差异显著。各家系组血浆LA指标呈先升高后降低的变化趋势,48 h家系组Ⅰ、Ⅳ的血浆LA显著低于其他家系组。攻毒后各家系组血浆转氨酶含量急剧上升,家系组Ⅰ、Ⅱ血浆ALT在24 h首先恢复到攻毒前水平。攻毒24 h后,肝脏中SOD水平达到顶峰,48 h家系组V肝脏中SOD水平最高,显著高于其他家系组。肝脏MDA水平呈逐渐升高的变化趋势,攻毒后6 h,家系组Ⅰ肝脏的MDA水平显著低于其他组,48 h家系组Ⅲ肝脏的MDA显著高于家系组Ⅰ、Ⅱ、Ⅳ。结果表明:各家系组表现出明显的免疫差别。家系组I(G♂×G♀)表现出较强的抗应激、非特异性免疫、肝脏抗氧化能力,在抗嗜水气单胞菌方面表现出抗病优势。

关键词：黄颡鱼(Pelteobagrus fulvidraco) 家系嗜水气单胞菌注射免疫指标

The impact of nitrogen amendment and crop growth on dissolved organic carbon in soil solution

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Journal of Mountain Science 2016年第1期13卷 95-103页

作者： WANG xiao-guo LI Chang-sheng LUO Yong hua Ke-ke ZHOU ming-hua The Key Laboratory of Mountain Environment Evolution and its Regulation Institute of Mountain Hazards and Environment ResearchChinese Academy of Sciences Institute for the Study of Earth Ocean and SpaceUniversity of New Hampshire Key Laboratory of Geosciences Spatial Information Technology Ministry of Land and Resources of the ChinaChengdu University of Technology Soil and Fertilizer Research Institute Anhui Academy of Agricultural Sciences Institute for Meteorology and Climate Research Atmospheric Environmental Research (IMK-IFU)Karlsruhe Institute of Technology

Dissolved organic carbon(DOC) is an important component of the terrestrial carbon ***,the sources and controlling factors of DOC in soils remain *** this study,the effects of nitrogen(N) amendment and crop growth on DOC in soil solution were examined at a maize-wheat rotated field located in the central Sichuan Basin in southwestern *** treatments in this study included 150 kg N ha-1 season-1,200 kg N ha-1 season-1 and the control without any fertilizer *** the whole experimental period,we observed significant decreases(p<0.05) in DOC concentrations in the sampled soil solutions associated with increase in N inputs at the bare soil plots,but no change in DOC at the plots with crop *** estimated average contributions of plantderived DOC were 16%,24% and 32% of total DOC in the summer maize season and 21%,32% and 38% in the winter wheat season along with the gradient of N fertilizer application *** results implied thatthe crop growth could play a key role in the soil DOC production,and the N input enhanced DOC production by increasing crop *** relationship between the DOC concentrations and the crop root biomass was statistically significant for both the maize and winter wheat *** observations indicated that crop growth exerted greater influence on the seasonal variability of DOC concentration in soil solutions at the experimental site,which overwhelmed the effect of soil native organic matter decomposition on DOC concentrations in soil solutions.

关键词： season winter amendment maize wheat fertilizer dissolved uncertain biomass exerted

Effect of Nitrogen Starvation on the Responses of Two Rice Cultivars to Nitrate Uptake and Utilization

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Pedosphere 2014年第5期24卷 690-698页

作者： YIN xiao-ming LUO Wei WANG Song-Wei SHEN Qi-Rong LONG xiao-hua College of Resources and Environmental Sciences Nanjing Agricultural University

Ammonium(NH+4) is the main nitrogen(N) form for rice crops, while NH+4near the root surface can be oxidized to nitrate(NO-3)by NH+4-oxidizing bacteria. Nitrate can be accumulated within rice tissues and reused when N supply is insufficient. We compared the remobilization of NO-3stored in the tissue and vacuolar between two rice(Oryza sativa L.) cultivars, Yangdao 6(YD6, indica)with a high N use efficiency(NUE) and Wuyujing 3(WYJ3, japonica) with a low NUE and measured the uptake of NO-3, expression of nitrate reductase(NR), NO-3transporter genes(NRTs), and NR activity after 4 d of N starvation following 7-d cultivation in a solution containing 2.86 mmol L-1NO-3. The results showed that both tissue NO-3concentration and vacuolar NO-3activity were higher in YD6 than WYJ3 under N starvation. YD6 showed a 2- to 3-fold higher expression of OsNRT2.1 in roots on the 1st and 4th day of N starvation and had significantly higher values of NO-3uptake(maximum uptake velocity, Vmax) than the cultivar ***, YD6 had significantly higher leaf and root maximum NR activity(NRAmax) and actual NR activity(NRAact) as well as stronger root expression of the two NR genes after the 1st day of N starvation. There were no significant differences in NRAmax and NRAact between the two rice cultivars on the 4th day of N starvation. The results suggested that YD6 had stronger NRA under N starvation, which might result in better NO-3re-utilization from the vacuole, and higher capacity for NO-3uptake and use, potentially explaining the higher NUE of YD6 compared with WYJ3.

关键词： nitrate reductase activity nitrate transpoters nitrogen use efficiency Oryza sativa L. remobilization

BMP-4 induced proliferation and oriented differentiation of rat hepatic oval cells into hepatocytes

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Asian Pacific Journal of Tropical Medicine 2015年第5期8卷 412-416页

作者： Zhi-ming Wang xiao-hua Yuan Hong Shen Department of Hepatobiliary Surgery Xiangya HospitalCentral South University Institute of Medical Science Xiangya HospitalCentral South University

Objective:To explore the role of bone morphogenetic protein 4(BMP-4) in hepatic progenitor cells(HPCs).Methods:The effect of BMP-4 on rat hepatic oval cells was examined by using the WB-F344 rat hepatocytic epithelial stem-cell-like cell *** hepatocytic cell line could exert various hepatocytc functions including the secretion of albumin and *** was used to examine the effects of BMP-4 and its antagonist,Noggin,on the proliferation and differentiation of these cells,cellular uptake and excretion of indocyanine green,the periodic acid-schiff(PAS) assay for glycogen storage and the expression of hepatic ***:Our results showed for the first time that BMP-4 may acted as a potential inducer of hepatic differentiation in rat hepatic oval ***:This cell source offers a much-needed attractive and expandable source for future investigations of drug screening,stem cell technologies and cellular transplantation,in a society with increasing levels of liver disease and damage.

关键词： Bone morphogenetic protein-4 Transforming growth factor-β Hepatic progenitor cells Proliferation Differentiation

Anomalous Resistivity Associated with Secondary Islands in the Reconnection Region

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Chinese Physics Letters 2012年第8期29卷 309-312页

作者： ZHOU Meng SU Wei DENG xiao-hua YAO ming Institute of Space Science and Technology Nanchang UniversityNanchang 330031 State Key Laboratory of Space Weather Chinese Academy of SciencesBeijing 100190 School of Electronic and Information Wuhan UniversityWuhan 430072

We calculate the anomalous resistivity(AR)due to electrostatic waves,including possibly the lower hybrid wave and electron beam mode,around the secondary islands in the reconnection region observed by the Cluster *** main findings are:AR is important on the reconnection separatrix layer but heavily suppressed at the central current sheet where B_(x)~***,there is a highly asymmetric pattern of AR across the island along the outflow direction,with much larger AR on one side of island than on the other *** results may be helpful in understanding the role of AR in reconnection.

关键词： island reconnection Resistivity

Modulation of Electrochemical Oscillations by Specific Adsorption of Cl- during the Electrooxidation of Methanol on Pt Electrode

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Chinese Chemical Letters 2002年第3期13卷 277-278页

作者： Ze Lin LI Ji hua huaNG xiao ming xiao Yue ZENG Xia CHU Department of Chemistry Hunan Normal UniversityChangsha 410081

Potential oscillation during the electrocatalytic oxidation of methanol can be modulated by the specific adsorption of Cl- on the platinum electrode, which suppresses the electrocatalytic oxidation of methanol, and makes the cross cycle in the cyclic voltammogram become smaller and finally disappear with the increase of Cl- concentration. The method is also applicable to the electrocatalytic oxidation of other small organic molecules.

关键词： Methanol chloride ions specific adsorption potential oscillation modulation.

Theoretical investigation of total-asymmetric simple exclusion processes with attachment and detachment

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Chinese Physics B 2012年第2期21卷 218-223页

作者： xiao Song Liu ming-Zhe Shang Jing Wang hua Faculty of Metallurgical and Energy Engineering Kunming University of Science and TechnologyKunming 650093China College of Nuclear Technology and Automation Engineering Chengdu University of TechnologyChengdu 610059China

In this paper, traffic systems with attachment and detachment have been studied by total-asymmetric simple exclusion processes （TASEPs）. Attachment and detachment in a one-dimensional system is a type of complex geometry that is relevant to biological transport with the random update rule. The analytical results are presented and have shown good agreement with the extensive Monte Carlo computer simulations.

关键词： total-asymmetric simple exclusion process attachment and detachment mean-field approach Monte Carlo simulations

Advances in Graphene‑Based Electrode for Triboelectric Nanogenerator

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Nano-Micro Letters 2025年第1期17卷 378-403页

作者： Bin Xie Yuanhui Guo Yun Chen Hao Zhang Jiawei xiao Maoxiang Hou Huilong Liu Li Ma Xin Chen Chingping Wong State Key Laboratory of Precision Electronic Manufacturing Technology and Equipment Guangdong University of TechnologyGuangzhou 510006People’s Republic of China School of Electromechanical Engineering Guangdong University of TechnologyGuangzhou 510006People’s Republic of China School of Materials Science and Engineering Georgia Institute of TechnologyAtlantaGA 30332USA

With the continuous development of wearable electronics,wireless sensor networks and other micro-electronic devices,there is an increasingly urgent need for miniature,flexible and efficient nanopower generation *** nanogenerator(TENG)technology can convert small mechanical energy into electricity,which is expected to address this *** the core component of TENG,the choice of electrode materials significantly affects its *** metal electrode materials often suffer from problems such as durability,which limits the further application of ***,as a novel electrode material,shows excellent prospects for application in TENG owing to its unique structure and excellent electrical *** review systematically summarizes the recent research progress and application prospects of TENGs based on graphene *** precision processing methods of graphene electrodes are introduced,and the applications of graphene electrode-based TENGs in various scenarios as well as the enhancement of graphene electrodes for TENG performance are *** addition,the future development of graphene electrode-based TENGs is also prospectively discussed,aiming to promote the continuous advancement of graphene electrode-based TENGs.

关键词： Triboelectric nanogenerator Precision processing Graphene electrode Self-powered sensor

新疆维吾尔族人群高尿酸血症与肥胖关系的探讨

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新疆医科大学学报 2009年第2期32卷 125-127页

作者：赵文婷姚华肖明丁丽丽新疆医科大学公共卫生学院新疆乌鲁木齐830011 新疆医科大学第一附属医院新疆乌鲁木齐830011

目的:探讨新疆维吾尔族人群高尿酸血症与肥胖及血脂的相关性。方法:在新入院就诊的维吾尔族患者中随机选择306例高尿酸血症者作为病例组,同时选择326例非高尿酸血症者作为对照组,分别进行腰围、臀围的测量和肾功、血脂的检测。结果:(1)... 详细信息

目的:探讨新疆维吾尔族人群高尿酸血症与肥胖及血脂的相关性。方法:在新入院就诊的维吾尔族患者中随机选择306例高尿酸血症者作为病例组,同时选择326例非高尿酸血症者作为对照组,分别进行腰围、臀围的测量和肾功、血脂的检测。结果:(1)病例组体质指数(BMI)、腰臀比(WHR)、尿素氮(BUN)、肌酐(Crea)和甘油三酯(TG)水平均高于对照组(P<0.05);男性病例组胆固醇(TC)和载脂蛋白B(ApoB)水平也较对照组增高(P<0.05);(2)男性的尿酸水平和BMI、TG、TC呈正相关关系(P<0.05);女性的尿酸水平与BMI、TG、WHR呈正相关关系(P<0.05);(3)男性病例组发生肥胖、腹型肥胖、高甘油三酯血症和高胆固醇血症的频率均高于对照组(P<0.05),而女性病例组肥胖发生率以及高密度脂蛋白血症的发生率高于对照组(P<0.05)。结论:新疆维吾尔族人群高尿酸血症与肥胖、高脂血症密切相关,其中血尿酸水平与血脂的联系程度在不同性别间略有差异,男性TG、TC水平和血尿酸水平的联系较女性更为密切。

关键词：高尿酸血症肥胖维吾尔族