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Effect of local viral transfer of interleukin 10 gene on a rabbit arthritis model induced by interleukin 1β

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Chinese Medical Journal 2008年第5期121卷 435-438页

作者： ZHANG Ning CUI hua-dong xue Hong-xia Department of Rheumatology and Immunology Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China

Backgroud Interleukin 16 （IL-1β） is the principal mediator in the pathogenesis of rheumatoid arthritis. Continuous injection of interleukin 1β （IL-1β） into the knee articular cavities of anamals can induce models that resemble rheumatoid arthritis. The objective of this study was to evaluate the feasibility of local recombinant retrovirus viral interleukin 10 （rRV-vlL-10） gene transfer treatment of a rabbit model of arthritis induced by IL-16. Methods An hlL-1β-induced rabbit rheumatoid arthritis model was established using the MFG-hlL-1β-neo-HIG-82 cell line, which is capable of continuous secretion of hlL-1β. After transfecting the rabbit synovial fibroblast cell line （MFG-hlL-1β-neo-HIG-82） with rRV-vlL-10, G418 was then added to identify the positive clone. The rRV-vlL-10 positive clone was injected into the established rabbit rheumatoid arthritis model through intra-articular injection. Successful gene transfer was determined by reverse transcripUon-polymerase chain reaction （RT-PCR） and immunohistochemistry. The levels of IL-1β before and after treatment were determined by enzyme- linked immunosorbent assay. Results Retrovirus vector was an effective vector both to synoviocytes in vitro and synovium tissue in vivo as confirmed by RT-PCR and immunohistochemistry. The rabbit arthritis model treated with rRV-vlL-10 showed a dramatic remission of arthritis and a decline in the level of cytokines such as IL-1β. Conclusions Retrovirus-mediated transfecUon of vlL-10 successfully transferred the gene into rabbit synovium ex vivo and was able to suppress intra-articular inflammation response to IL-1β.

关键词： arthritis, rheumatoid gene therapy animal model retrovirus interleukin

Resonance mechanism of flapping wing based on fluid structure interaction simulation

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Chinese Journal of Aeronautics 2024年第5期37卷 243-262页

作者： Yueyang GUO Wenqing YANG Yuanbo dong dong xue National Key Laboratory of Science and Technology on Aerodynamic Design and Research School of AeronauticsNorthwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518063China Yangtze River Delta Research Institute of Northwestern Polytechnical University Taicang 215400China

Certain insect species have been observed to exploit the resonance mechanism of their *** order to achieve resonance and optimize aerodynamic performance,the conventional approach is to set the flapping frequency of flexible wings based on the Traditional Structural Modal(TSM)***,there exists controversy among researchers regarding the relationship between frequency and aerodynamic *** that the structural response of wings can be influenced by the surrounding air vibrations,an analysis known as Acoustic Structure Interaction Modal(ASIM)is introduced to calculate the resonant *** this study,Fluid Structure Interaction(FSI)simulations are employed to investigate the aerodynamic performance of flapping wings at modal frequencies derived from both TSM and ASIM *** performance is evaluated for various mass ratios and frequency ratios,and the findings indicate that the deformation and changes in vortex structure exhibit similarities at mass ratios that yield the highest aerodynamic ***,the flapping frequency associated with the maximum time-averaged vertical force coefficient at each mass ratio closely aligns with the ASIM frequency,as does the frequency corresponding to maximum ***,the ASIM analysis can provide an effective means for predicting the optimal flapping frequency for flexible ***,it enables the prediction that flexible wings with varying mass ratios will exhibit similar deformation and vortex structure *** paper offers a fresh perspective on the ongoing debate concerning the resonance mechanism of Flexible Flapping Wings(FFWs)and proposes an effective methodology for predicting their aerodynamic performance.

关键词： Flexible Flapping Wing(FFW) Acoustic Structure Interaction Modal(ASIM) Fluid Structure Interaction(FSI) Resonance mechanism Aerodynamic performance

Nano polystyrene microplastics could accumulate in Nile tilapia(Oreochromis niloticus):Negatively impacts on the intestinal and liver health through water exposure

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Journal of Environmental Sciences 2024年第3期137卷 604-614页

作者： Di Wu Xing Lu Li-xue dong Juan Tian Jin Deng Lei Wei hua Wen Shan Zhong Ming Jiang School of Basic Medical Sciences Wuhan UniversityWuhan 430071China Key Laboratory of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural AffairsYangtze River Fisheries Research InstituteChinese Academy of Fishery SciencesWuhan 430223China Hubei Province Key Laboratory of Allergy and Immunology Wuhan 430071China

Microplastics(MPs)have become a significant concern for their potential ***,the correlation between the size of plastic particles and their toxicity remains ***,we investigate the toxic effects of different sizes(80 nm,800 nm,8μm and 80μm)polystyrene MPs(PS-MPs)on the model organism Nile tilapia(Oreochromis niloticus).The results of bioluminescent imaging indicate that the 80 nm PS-MPs are more likely to invade the body.H&E staining shows severe damage on the intestinal villi and distinct hepatic steatosis in the 80 nm *** U labeling shows that the proliferation activity of intestinal and liver cells reduces significantly in the 80 nm *** gut microbiome analysis shows a severe imbalance of gut microbiota homeostasis in the 80 nm *** analysis of liver transcriptomics and metabolomics shows that the liver lipid metabolism is disordered in the 80 nm *** conclusion,this study confirms that the 80 nm PS-MPs are more likely to induce intestinal and liver *** the above lay the foundation for further study on the pathological damage of MPs to other organisms.

关键词： Microplastics(MPs) Intestinal toxicity Liver toxicity Gut microbiome Lipid metabolism

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考虑车与车互联通讯技术的交通流跟驰模型

物理学报 2016年第1期65卷 44-55页

作者：华雪东王炜王昊东南大学交通学院江苏省城市智能交通重点实验室南京210096 现代城市交通技术江苏高校协同创新中心南京210096

基于Newell跟驰模型,建立考虑车与车互联(vehicle-to-vehicle,V2V)通讯技术的单车道跟驰模型.根据V2V技术的特征,引入参数α以表征驾驶员在收到V2V技术所提供的实时交通信息后的提前反应程度.根据线性稳定分析方法,得到V2V跟驰模型的中... 详细信息

基于Newell跟驰模型,建立考虑车与车互联(vehicle-to-vehicle,V2V)通讯技术的单车道跟驰模型.根据V2V技术的特征,引入参数α以表征驾驶员在收到V2V技术所提供的实时交通信息后的提前反应程度.根据线性稳定分析方法,得到V2V跟驰模型的中性稳定条件.通过计算机的模拟,研究V2V技术对交通流运行的影响,分析小扰动下V2V跟驰模型对参数变化的敏感性,研究不同α取值下交通流密度波及迟滞回环的变化.研究发现:1)与全速度差跟驰模型相比,在引入V2V后,交通流在加速起步、减速刹车及遇到突发事件时,车辆运行的安全性和舒适性均得到不同程度的提升;2)V2V跟驰模型对参数α及T的变化较为敏感,且在交通流较为拥堵时,V2V技术的引入可以提升交通流的平均速度;3)参数α的增大、T的减小可以有效提升V2V跟驰模型在不同交通环境下的运行稳定性.由于可以实时地获取交通流运行的状态并针对性地改变车辆自身的运行,V2V交通流跟驰模型提升了交通流运行的稳定性.

关键词：跟驰模型车与车互联技术线性分析数值模拟

考虑自适应巡航车辆影响的上匝道系统混合交通流模型

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物理学报 2016年第8期65卷 219-231页

作者：华雪东王炜王昊东南大学交通学院江苏省城市智能交通重点实验室南京210096 现代城市交通技术江苏高校协同创新中心南京210096

在考虑自适应巡航(adaptive cruise control,ACC)车辆的交通流模型的基础上,建立了考虑ACC车辆影响的上匝道系统混合交通流模型,研究ACC车辆引入对上匝道交通系统交通流的影响.为了描述ACC车辆和手动驾驶车辆在交通流运行中的差异,分别... 详细信息

在考虑自适应巡航(adaptive cruise control,ACC)车辆的交通流模型的基础上,建立了考虑ACC车辆影响的上匝道系统混合交通流模型,研究ACC车辆引入对上匝道交通系统交通流的影响.为了描述ACC车辆和手动驾驶车辆在交通流运行中的差异,分别构建了基于常车头时距原则的ACC车辆跟驰子模型和手动驾驶车辆MCD元胞自动机子模型;基于上匝道车辆合流驶入主线的需求,建立了换道子模型,引入了表征驾驶员换道心理的参数λ.通过对混合交通流模型进行数值模拟发现,ACC车辆的混入可以有效改善上匝道系统交通流的运行,降低合流等事件对于交通流运行的影响,抑制交通拥堵的时空范围及拥堵强度,提高交通流的平均速度和流量.此外在混合交通流模型中,ACC车辆期望车头时距Hd的减小与换道心理参数λ的增大均可以提高混合交通流运行的速度和流量,而合流区长度lw对混合交通流影响则因上匝道车辆驶入概率的不同而存在差异.

关键词：自适应巡航车辆混合交通流模型跟驰模型元胞自动机模型

Effect of electron-electron interaction on polarization process of exciton and biexciton in conjugated polymer

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Chinese Physics B 2024年第3期33卷 556-566页

作者：李晓雪彭华王栋侯栋 School of Physics and Technology University of JinanJinan 250022China Jinan Institute of Quantum Technology Jinan 250101China

By using one-dimensional tight-binding model modified to include electron-electric field interaction and electron-electron interaction,we theoretically explore the polarization process of exciton and biexciton in *** dynamical simulation is performed by adopting the non-adiabatic evolution *** results show that under the effect of moderate electric field,when the strength of electron-electron interaction is weak,the singlet exciton is stable but its polarization presents obvious *** the enhancement of interaction,it is dissociated into polaron pairs,the spin-flip of which can be observed through modulating the interaction *** the triplet exciton,the strong electron-electron interaction restrains its normal polarization,but it is still *** the case of biexciton,the strong electron-electron interaction not only dissociate it,but also flip its charge *** yield of the possible states formed after the dissociation of exciton and biexciton is also calculated.

关键词： conjugated polymer exciton electron-electron interaction reverse polarization

Mechanical performance of 22SiMn2TiB steel welded with lowtransformation- temperature filler wire and stainless steel filler wire

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Journal of Iron and Steel Research International 2024年第4期31卷 967-981页

作者： Zi-dong Lin Kai-jie Song Zhen Sun Zi-qian Zhu xue-feng Zhao Constantinos Goulas Wei Ya Xing-hua Yu School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China Department of Design Production and Management Faculty of Engineering Technology.University of TwenteEnschede 7500 AETheNetherlands Rotterdam Additive Manufacture Fieldlab(RAMLAB) Rotterdam 3089 JWTheNetherlands

TX-80 low-transformation-temperature(LTT)welding wire was used to replace the traditional ER 307Si welding wire to realize the connection of 22SiMn2TiB armor steel in manual overlay *** previously existing issues,such as welding cracks,large welding deformation,and severe welding residual stress,were solved to ensure good strength and ductility *** particular,with the same welding conditions,TX-80 LTT wire eliminates welding *** reduces the welding deformation no matter the base pretreatment of pre-setting angle or no pre-setting *** comparison,it was found that the microstructure at the TX-80 weld is mainly composed of martensite and a small amount of retained *** contrast,the microstructure of the ER 307Si weld consists of a large amount of austenite and a small amount of skeleton-like *** variation trend of residual stress and microhardness from the weld to the base were investigated and compared with the mechanical properties of base *** TX-80 and the ER 307Si tensile samples elongation is 6.76%and 6.01%,while the ultimate tensile strengths are 877 and 667 MPa,*** average impact toughness at room temperature of the ER 307Si weld is 143.9 J/cm^(2),much higher than that of the TX-80 weld,which is only 36.7 J/cm^(2).The relationship between impact and tensile properties with microstructure species and distribution was *** addition,the fracture surface of the tensile and the impact samples was observed and *** dimples,fewer pores,larger radiation zone,and shear lips of TX-80 samples indicate better tensile ductility and worse impact toughness than those of ER 307Si weld.

关键词： Low-transformation-temperature material 22SiMn2TiB steel Welding deformation Residual stress Martensitic transformation Mechanical property

Breaking the efficiency limitations of dissipative Kerr solitons using nonlinear couplers

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Science China(Physics,Mechanics & Astronomy) 2024年第3期67卷 88-94页

作者： Ming Li Xiao-Xiao xue Yan-Lei Zhang Xin-Biao Xu Chun-hua dong Guang-Can Guo Chang-Ling Zou CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Centerfor Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China Department of Electronic Engineering Beijing National Research Center for Information Science and TechnologyTsinghua University Beijing 100084China

Dissipative Kerr solitons(DKS) have long been suffering from poor power conversion efficiency when driven by continuous-wave lasers. By deriving the critical coupling condition of a multimode nonlinear optics system in a generalized theoretical framework,two efficiency limitations of the conventional pump method of DKS are revealed: the effective coupling rate is too small and is also power-dependent. A general approach is provided to resolve this challenge by introducing two types of nonlinear couplers to couple the soliton cavity and CW input through nonlinear processes. The collective coupler opens multiple coupling channels and the self-adaptive coupler builds a power-independent effective external coupling rate to the DKS for approaching the generalized critical coupling condition, which promises near-unity power conversion efficiencies. For instance, a conversion efficiency exceeding 90% is predicted for aluminum nitride microrings with a nonlinear coupler utilizing second-harmonic generation. The mechanism applies to various nonlinear processes, including Raman and Brillouin scattering, and thus paves the way for micro-solitons toward practical applications.

关键词： Kerr frequency comb conversion efficiency nonlinear coupler

NQO1 Mediates Lenvatinib Resistance by Regulating ROS-induced Apoptosis in Hepatocellular Carcinoma

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Current Medical Science 2024年第1期44卷 168-179页

作者： Wei xue Ting WANG Wen-jing TIAN Si-qi PANG hua-feng ZHANG Wei-dong JIA Department of General Surgery The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China Institute of Health and Medicine Hefei Comprehensive National Science CenterHefei 230601China The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease School of Basic Medical SciencesDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China

Objective Hepatocellular carcinoma(HCC)is the third leading cause of cancer-associated death *** a first-line drug for advanced HCC treatment,lenvatinib faces a significant hurdle due to the development of both intrinsic and acquired resistance among patients,and the underlying mechanism remains largely *** present study aims to identify the pivotal gene responsible for lenvatinib resistance in HCC,explore the potential molecular mechanism,and propose combinatorial therapeutic targets for HCC *** Cell viability and colony formation assays were conducted to evaluate the sensitivity of cells to lenvatinib and ***-Seq was used to determine the differences in transcriptome between parental cells and lenvatinib-resistant(LR)*** upregulated genes were analyzed by GO and KEGG ***,qPCR and Western blotting were employed to determine the relative gene expression ***,the intracellular reactive oxygen species(ROS)and apoptosis were detected by flow *** PLC-LR and Hep3B-LR were *** was a total of 116 significantly upregulated genes common to both LR cell *** GO and KEGG analyses indicated that these genes were involved in oxidoreductase and dehydrogenase activities,and reactive oxygen species ***,NAD(P)H:quinone oxidoreductase 1(NQO1)was highly expressed in LR cells,and was involved in the lenvatinib *** high expression of NQO1 decreased the production of ROS induced by lenvatinib,and subsequently suppressed the *** combination of lenvatinib and NQO1 inhibitor,dicoumarol,reversed the resistance of LR *** The high NQO1 expression in HCC cells impedes the lenvatinib-induced apoptosis by regulating the ROS levels,thereby promoting lenvatinib resistance in HCC cells.

关键词： hepatocellular carcinoma lenvatinib resistance NAD(P)H quinone oxidoreductase 1 reactive oxygen species apoptosis dicoumarol