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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 677-682页

作者： hua tian~(1,2*) ioannis {3.~2 1 department of orthopaedics,peking university Third Hospital,beijing 100083.china 2 Coxa Hospital for Joint Replacement,Biokatu 6 b,Tampere 3.101,Finland department of orthopaedics Peking University Third HospitalBeijing 100083 China Coxa Hospital for Joint Replacement Biokatu 6 bTampere 33101 Finland

Cartilage oligomeric matrix protein (COMP) strengthens cartilage by binding to type Ⅱ and typeⅨ collagen-forming bridges between collagen *** was hypothesized that perhaps one or more anabolicgrowth factors such as insulin-like growth factor-Ⅰ (IGF-Ⅰ),fibroblast growth factor-1 (FGF-1) or plateletderived growth factor-BB (PDGF-BB) increase COMP gene *** effects on primary humanchondrocytes and the chondrogenic cell line ATDC5 were studied using real time reverse transcript-poly-merase chain reaction (RT-PCR) for ***-Ⅰ,but not the FGF-1 or PDGF-BB,up-regulatedCOMP gene expression by approximate 5-fold in human adult chondrocytes in a dose-and ***-Ⅰ exerted similar effects on ATDC5 *** from these real time RT-PCR experimentswere confirmed by transfecting into ATDC5 cells a full-length mouse COMP promoter cloned upstream of aluciferase reporter *** stimulation with IGF-Ⅰ,the luciferase reporter activity increased by about *** conclusion,IGF-Ⅰ seems to be an important positive regulator of COMP,which may play animportant role in an attempted repair of either traumatized or degenerated cartilage.

关键词： cartilage oligomeric matrix protein (COMP) insulin-like growth factor-Ⅰ(IGF-Ⅰ) gene expression

Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal stem and progenitor cell by adeno-associated virus vectors

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Acta Pharmacologica Sinica 2004年第2期25卷 70-76页

作者： Xiao-dong JU, Si-quan LOU2, Wei-guo WANG, Jian-qiang PENG3. hua tian department of {3., peking university Third Hospital, beijing 100083 3.nstitute of Virology, Chinese Academy of Preventive Medicine, beijing 100262, china department of orthopaedics Peking University Third Hospital Beijing 100083 China

AIM: To study the effect of hydroxyurea and etoposide on transduction of human marrow mesenchymal and progenitor stem cells by adeno-associated virus (AAV). METHODS: Isolated human bone marrow mesenchymal stem and progenitor cells (hMSCs) were cultured in DMEM containing 10 % FBS or 5 % FBS and dexamethasone 1 μmol/L respectively. After being treated with hydroxyurea and etoposide, hMSCs were transduced by AAV-LUC. After two days luciferase activity (relative light unites per second or RLU/s) were tested, which indirectly reflected the relative transduction efficiency of different groups, and virus DNA was isolated by Hirt extraction for Southern hybridization. RESULTS: Transduction luciferase activity and transduction efficiency in cultures treated with hydroxyurea and etoposide were significantly higher than that in control cultures. Dividing cells had about 20-fold higher transduction efficiencycompared with control cells. Transduction efficiency in stationary cells was about 50 times higher than that in control cells. Southern analysis showed that hydroxyurea and etoposide enhanced second-strand DNA synthesis by rAAV. CONCLUSION: Hydroxyurea and etoposide could increase transduction efficiency of hMSCs by AAV vectors, and stationary cells were more sensitive to these drugs than dividing cells.

关键词： simian adenoviruses hydroxyurea etoposide mesoderm stem cells gene transfer techniques

Cistanche Deserticola for Regulation of Bone Metabolism:Therapeutic Potential and Molecular Mechanisms on Postmenopausal Osteoporosis

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Chinese Journal of Integrative Medicine 2023年第1期29卷 74-80页

作者： WANG Cheng LI Feng LI Yang FENG Hui ZHAO Min-wei TU Peng-fei tian hua department of orthopaedics/Engineering Research Center of Bone and Joint Precision Medicine/beijing Key Laboratory of Spinal Disease Research Peking University Third HospitalBeijing100191China peking university School of Pharmaceutical Sciences Beijing100191China

Osteoporosis is a generalized disease of bone that leads to a loss of bone density and bone mass,destruction of bone microstructure,increased brittleness and therefore *** present,the main treatment of Western medicine is drug therapy such as bisphosphonates,calcitriol,vitamin D,***,long-term use of these drugs may bring some adverse *** herbal medicine Cistanche deserticola could regulate bone metabolism by promoting osteoblast activity and inhibiting osteoclast activity with low toxicity and adverse ***,Cistanche deserticola has attracted increasing attention for its efficacy in the prevention and treatment of osteoporosis in recent *** we present a literature review of the molecular pathways involved in osteoporosis and the effects of Cistanche deserticola on bone *** objective is to clarify the mechanism of Cistanche deserticola in the treatment of osteoporosis.

关键词： osteoporosis Cistanche deserticola bone metabolism Chinese medicine

Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized mice

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Acta Pharmaceutica Sinica B 2020年第7期10卷 1321-1330页

作者： Haifeng huang hua Zhu Quan Xie Xiaobin tian Xianteng Yang Fan Feng Qiyu Jiang Xinan Sheng Zhi Yang Guizhou university School of Medicine Guizhou UniversityGuiyang 550025China department of orthopaedics Guizhou Provincial People’s HospitalGuiyang 550002China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/beijing) Department of Nuclear MedicinePeking University Cancer Hospital&InstituteBeijing 100142China Research Center for Clinical and Translational Medicine the 302nd Hospital of Chinese PLABeijing 100039China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/beijing) Department of Renal Cancer and MelanomaPeking University Cancer Hospital&InstituteBeijing 100142China

JS001(toripalimab)is a humanized IgG monoclonal antibody which strongly inhibits programmed cell death protein 1(PD1).In this study,we used a different iodine isotype(nat/124/125I)to label JS001 probes to target the human PD1(hPD1)*** vitro,the half maximal effective concentration(EC50)value of natI-JS001 did not significantly differ from that of *** uptake of 125I-JS001 by activated T cells was 5.63.times higher than that by nonactivated T cells after 2 h of *** binding affinity of 125I-JS001 to T cells of different lineages after phytohemagglutinin(PHA)stimulation reached 4.26 nmol/*** PD1 C57 BL/6 mice bearing mouse sarcoma S180 cell tumors were validated for immuno-positron emission tomography(immuno-PET)*** staining was used to assess the expression of PD1 in tumor *** homologous 124I-human IgG(124I-hIgG)group or blocking group was used as a control ***-PET imaging showed that the uptake in the tumor area of the 124I-JS001 group at different time points was significantly higher than that of the blocking group or the 124I-hIgG group in the humanized PD1 mouse *** together,these results suggest that this radiotracer has potential for noninvasive monitoring and directing tumor-specific personalized immunotherapy in PD1-positive tumors.

关键词： Immunotherapy JS001 Toripalimab Programmed cell death protein 1 Immuno-PET imaging Iodine isotopes

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机器人辅助人工髋膝关节置换手术是必然趋势

中华医学杂志 2022年第1期102卷 4-8页

作者：田华北京大学第三医院骨科骨与关节精准医学教育部工程研究中心北京100191

人工髋、膝关节置换是骨科领域最成功的手术之一,能有效缓解疼痛、矫正畸形和改善功能,从而提高患者整体生活质量。尽管髋、膝关节置换是目前治疗晚期髋、膝疾患的常规手术,但依然存在着术后肢体不等长、脱位或不稳定、跛行和不明原因... 详细信息

人工髋、膝关节置换是骨科领域最成功的手术之一,能有效缓解疼痛、矫正畸形和改善功能,从而提高患者整体生活质量。尽管髋、膝关节置换是目前治疗晚期髋、膝疾患的常规手术,但依然存在着术后肢体不等长、脱位或不稳定、跛行和不明原因疼痛等并发症。这些并发症的发生很大程度上与假体安放的位置相关。机器人辅助进行髋、膝关节置换手术的优势不仅在于术前可进行三维手术规划,而且手术过程中机械臂能够辅助完美实现术前规划,避免了人为误差。目前的关节置换机器人依然存在着不能进行个性化手术规划、手术时间长效率低、价格昂贵等问题,但相信在人工智能加持和算法的不断优化下,人工关节置换机器人将会越来越精准化、智能化和微创化,这是关节外科未来发展的必然趋势。

关键词：关节成形术机器人辅助手术髋关节置换术膝关节置换术

Relationship between quantitative magnetic resonance imaging and clinical symptoms in patients with knee osteoarthritis

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Chinese Medical Journal 2020年第14期133卷 1741-1743页

作者： Xin-Guang Wang Li-Xiang Gao Min-Wei Zhao Xiao Geng tian-Chen Wu Hui-Shu Yuan hua tian department of orthopaedics Peking University Third HospitalBeijing 100191China department of Radiology Peking University Third HospitalBeijing 100191China School of Public Health Peking UniversityBeijing 100191China

To the Editor:Quantitative magnetic resonance imaging(MRI)objectively evaluates the degeneration of cartilage in knee osteoarthritis(KOA)patients by measuring changes in proteoglycans,collagen fibers,and water content,thereby facilitating early clinical diagnosis and treatment.[1]Western Ontario and MacMaster Universities(WOMAC)osteoarthritis index score is used to evaluate the symptoms of KOA patients.[2]Presently,few studies have evaluated the correlation between quantitative MRI findings and clinical symptoms(eg.,study performed by Zarins et al[3.).This study explored the relationship between quantitative MRI in T1rho and T2-mapping sequence and WOMAC osteoarthritis index scores of KOA patients.

关键词： patients clinical symptoms

Trends and characteristics in pre-hospital emergency care in beijing from 2008 to 2017

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Chinese Medical Journal 2020年第11期133卷 1268-1275页

作者： Wei huang tian-Bing Wang Ying-Dong He huan Zhang Xiao-hua Zhou Hui Liu Jin-Jun Zhang Zhen-Biao tian Bao-Guo Jiang Departm ent of Trauma Rescue Center Peking University People's HospitalBeijing 100044China department of Trauma and orthopaedics Peking University People's HospitalBeijing 100044China Key Laboratory of Trauma and Neural Regeneration(peking university) Ministry of EducationBeijing 100044China department of Epidemiology and Biostatistics School of Public HealthPeking University Health Science CenterBeijingChina Medical Informatics Center Peking UniversityBeijing 100191China beijing Emergency Center Beijing 100031China beijing Red Cross Emergency Center Beijing 100085China

Background: We aimed to describe and analyze the pre-hospital emergency medical service (EMS) in beijing and provide information for the government and medical institutions to optimize ***: We collected all pre-hospital emergency data in beijing from 2008 to 2017. The chief complaint in each case was classified according to the Medical Priority Dispatch System (MPDS). The sites’ administrative districts were determined through geo-encoding of addresses and then classified into four functional regions. We analyzed the demand for EMS, emergency response times (ERT), and disease spectrum for beijing as a whole, and for each functional ***: A total of 4,192,870 pre-hospital EMS cases met the inclusion criteria, with a significant increase (P 2008 to 2017. EMS demand was positively associated with population (r= 0.946,P 2008 and 2172.23.in 2017 per 100,000, with no significant change (P = 0.57). ERT increased significantly (P = 0.001), from 19.18 min in 2008 to 24.51 min in 2016. According to MPDS classifications, the demand for pre-hospital care increased for 14 diseases, remained stable for 19, and decreased for only 1 disease. Cases of injury-related disease increased significantly from approximately 90,000 in 2017, accounting for 20% of all pre-hospital EMS cases, and the demand rate decreased in the core region but increased in the sub-urban regions. Cases of heart problems and stroke/transient ischemic attack also increased significantly in the four functional regions, with the highest demand rate in the Core Functional ***: More resources and effort should be devoted to pre-hospital EMS according to the increased pre-hospital EMS demand and prolonged ERT in beijing over our 10-year study period. Changes in disease spectrum and differences between functional regions should also be considered.

关键词： china Emergency medical services Emergency response time

计算机导航系统、3.打印截骨导板与传统器械对全膝关节置换术手术时间和下肢力线恢复的影响

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中华医学杂志 2018年第27期98卷 2157-2161页

作者：李杨田华耿霄北京大学第三医院骨科 100191

目的分析在全膝关节置换术（TKA）中使用计算机导航（CAS）系统、3.打印个性化截骨导板（PSI）与传统器械手术在手术时间和术后下肢冠状面机械轴力线恢复上的差异。方法回顾性收集2013.1月至2014年12月在北京大学第三医院诊断为重度骨... 详细信息

目的分析在全膝关节置换术（TKA）中使用计算机导航（CAS）系统、3.打印个性化截骨导板（PSI）与传统器械手术在手术时间和术后下肢冠状面机械轴力线恢复上的差异。方法回顾性收集2013.1月至2014年12月在北京大学第三医院诊断为重度骨性关节炎，经同一手术医师完成的单侧、初次TKA患者共96例。按照手术方式分为3.：CAS-TKA组于术中使用CAS进行TKA手术，PSI-TKA组术中使用PSI进行TKA手术，对照组使用传统器械进行TKA手术。比较3.患者的手术时间和术后下肢冠状面机械轴力线的差异。3.间比较采用单因素方差分析，组间两两比较采用LSD法检验。结果所有患者TKA手术时间为（79±16） min。其中，CAS-TKA组时间最长，明显长于其他2组[CAS-TKA比PSI-TKA：（90±10） min比（80±14） min，LSD-t＝3.458，P〈0.05；CAS-TKA比对照组：（90±10） min比（66±11） min，LSD-t＝8.3.0，P〈0.05；PSI-TKA组比对照组（80±14） min比（66±11） min，LSD-t＝4.582，P〈0.05]。所有患者术后下肢冠状面机械轴力线平均内外翻角度分别为2.4°和2.0°。总体力线偏移（内翻/外翻〉3.）率是22.9%，其中CAS-TKA组为11.8%，PSI-TKA组为27.6%，对照组为3..3.，3.力线偏移率差异无统计学意义（χ2＝3.771，P＝0.151）。结论CAS和PSI的使用均会显著增加TKA手术时间，和传统器械手术一样，这两种技术均可良好地恢复TKA术后下肢冠状位力线，也展现出了减少力线偏移的趋势。

关键词：关节成形术置换膝计算机导航辅助手术 3.打印个性化截骨导板下肢力线

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止血带在全膝关节置换术中应用的研究进展

中华骨与关节外科杂志 2023年第7期16卷 667-672页

作者：董子漾李杨田华北京大学第三医院骨科北京100191 骨与关节精准医学教育部工程研究中心北京100191

目前,止血带在全膝关节置换术(TKA)中的应用仍存在争议。止血带虽具有减少术中失血量、缩短手术时间等优势,但也存在导致术后额外疼痛肿胀、延长住院时间、增加并发症发生等风险。使用控制性降压及氨甲环酸等手段已经可以有效控制术中出... 详细信息

目前,止血带在全膝关节置换术(TKA)中的应用仍存在争议。止血带虽具有减少术中失血量、缩短手术时间等优势,但也存在导致术后额外疼痛肿胀、延长住院时间、增加并发症发生等风险。使用控制性降压及氨甲环酸等手段已经可以有效控制术中出血,因此多数学者支持TKA术中不常规使用止血带。本文从使用方法、相关问题及替代方法等方面对止血带在TKA术中应用的研究进展进行综述,为临床上止血带的使用提供参考。

关键词：全膝关节置换术止血带加速康复

机器人辅助髋膝关节置换术的应用现状及趋势分析

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中华骨与关节外科杂志 2023年第4期16卷 378-384页

作者：何宜蓁耿霄田华北京大学第三医院骨科北京100191 骨与关节精准医学教育部工程中心北京100191

手术机器人技术在关节外科领域的应用日益广泛。有研究表明手术机器人在提高人工关节置换术(TJA)精准度上有一定优势,但同时存在手术时间延长、医疗费用成本增加等局限性。有必要了解机器人辅助TJA的应用现状,思考其未来的发展趋势。本... 详细信息

手术机器人技术在关节外科领域的应用日益广泛。有研究表明手术机器人在提高人工关节置换术(TJA)精准度上有一定优势,但同时存在手术时间延长、医疗费用成本增加等局限性。有必要了解机器人辅助TJA的应用现状,思考其未来的发展趋势。本文将对关节置换手术机器人的发展历程和国内外关节置换手术机器人系统的临床应用现状进行文献综述,分析关节置换手术机器人的优势及不足,并探讨其未来的发展趋势,为今后关节置换手术机器人的研发和临床研究提供参考。

关键词：机器人全髋关节置换术全膝关节置换术单髁置换术