检索结果-南通市图书馆

Establishment of a humanized ST6GAL1 mouse model for influenza research

Animal Models and Experimental Medicine 2024年第3期7卷 337-346页

作者： Lyu Chao Han Feng Gao Qian Lv Limin Lu Ziwei Lu Shuangshuang Li Xiaoyan hu yuechao Yang Mengjie Zhao Yingze Liu Jun Lu Xuancheng Duo Shuguang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Institute of Zoology Chinese Academy of SciencesBeijingChina NHC Key Laboratory of Biosafety National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Zhejiang Provincial Key Laboratory of Medical Genetics Key Laboratory of Laboratory MedicineMinistry of EducationSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhouChina

Background:This study aimed to construct and characterize a humanized influenza mouse model expressing ***:humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of *** manipulated embryos were transferred into the oviducts of pseudopregnant female *** offspring were identified using *** exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for *** of the humanized gene was tested by performing immunohistochemical(IHC)*** and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were ***:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT *** mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection *** IHC results revealed that hST6GAL1 was expressed in the lungs of humanized ***,the hematologic and biochemical parameters of the positive mice were within the normal ***:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.

关键词： hST6GAL1 humanized mice influenza animal model

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Significant changes in the physicochemical properties of BC-containing particles during the cold season in Beijing

引用

Journal of Environmental Sciences 2025年第5期151卷 667-676页

作者： Shuya hu Linghan Zeng Gang Zhao Shiyi Chen Chunsheng Zhao Weilun Zhao Min hu State Key Joint Laboratory of Environmental Simulation and Pollution Control International Joint Research Center for Atmospheric Research(IJRC)College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China Department of Atmospheric and Oceanic Sciences School of PhysicsPeking UniversityBeijing 100871China Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Nanjing University of Information Science and TechnologyNanjing 210044China

The ambient air quality has improved significantly under strict emission controls in Beijing,China over the last *** carbon(BC),as a short-lived climate forcer in ambient aerosols,profoundly impacts the air quality and *** studies have demonstrated a decline in the mass concentration of *** this study,we characterized the chemical compositions and size distributions of BC-containing particles during the cold season of 2022 in Beijing using state-of-the-art instruments capable of exclusively measuring BCcontaining *** optical properties of BC-containing particles were further calculated based on the Mie ***,we compared the properties of BC-containing particles in 2022 with the results of previous *** results showed that the diameters of BC cores became larger while the coating thickness of BC-containing particles became thinner in *** the coating materials,the mass fraction of nitrate increased obviously,and even replaced organic matter as the dominant component during the peak of the pollution *** in chemical compositions and size distributions resulted in lower mass absorption cross-sections(MAC)of BC-containing particles from 10.5±1.1 m^(2)/g in 2016 to 7.3±0.8 m^(2)/g in 2022,reduced by 30.5%.Our results demonstrate the synergistic benefits of air pollution control in improving air quality and mitigating climate ***,the MAC of BC adopted in climate models should vary with the changing air pollution *** study emphasizes that it is imperative to conduct long-term observations of BC-containing particles to better estimate BC’s climate effects.

关键词： Black carbon Chemical composition Size distribution Light absorption ability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Degradation of carbamazepine by the UVA-LED_(365)/ClO_(2)/NaClO process:Kinetics,mechanisms and DBPs yield

引用

Journal of Environmental Sciences 2025年第2期148卷 399-408页

作者： Chenyan hu Yihui Wu Zhengyu Dong Ziyi Dong Shengjie Ji Lili hu Xinyu Yang Hao Liu College of Environmental and Chemical Engineering Shanghai Engineering Research Center of EnergySaving in Heat Exchange SystemsShanghai University of Electric PowerShanghai 200090China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092China

A mixed oxidant of chlorine dioxide(ClO_(2))and NaClO was often used in water treatment.A novel UVA-LED(_(365) nm)-activated mixed ClO_(2)/NaClO process was proposed for the degradation of micropollutants in this ***(CBZ)was selected as the target *** with the UVA_(365)/ClO_(2) process,the UVA_(365)/ClO_(2)/NaClO process can improve the degradation of CBZ,with the rate constant increasing from 2.11×10^(−4) sec^(−1) to 2.74×10^(−4) sec^(−1).In addition,the consumption of oxidants in the UVA_(365)/ClO_(2)/NaClO process(73.67%)can also be lower than that of UVA_(365)/NaClO(86.42%).When the NaClO ratio increased,both the degradation efficiency of CBZ and the consumption of oxidants can increase in the UVA_(365)/ClO_(2)/NaClO *** solution pH can affect the contribution of NaClO in the total oxidant *** the pH range of 6.0-8.0,the combination process can generate more active species to promote the degradation of *** change of active species with oxidant molar ratio was investigated in the UVA_(365)/ClO_(2)/NaClO *** ClO_(2) acted as the main oxidant,HO·and Cl·were the main active species,while when NaClO was the main oxidant,ClO·played a role in the *** chloride ion(Cl^(-)),bicarbonate ion(HCO_(3)^(-)),and nitrate ion(NO_(3)^(-))can promote the reaction *** the concentration of NaClO in the reaction solution increased,the generation of chlorates will *** UVA_(365)/ClO_(2)/NaClO process can effectively control the formation of volatile disinfection by-products(DBPs),and with the increase of ClO_(2) dosage,the formation of DBPs can also decrease.

关键词： UVA Chlorine dioxide(ClO_(2)) NaClO Carbamazepine(CBZ) Disinfection by-products

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nitrogen-cycling processes under long-term compound heavy metal(loids)pressure around a gold mine:Stimulation of nitrite reduction

引用

Journal of Environmental Sciences 2025年第1期147卷 571-581页

作者： Xuesong hu Xiaoxia Liu Shuo Zhang Caihong Yu School of Chemical&Environmental Engineering China University of Mining and Technology(Beijing)Beijing 100083China Beijing Cultivated Land Construction and Protection Center Beijing 100020China

Mining and tailings deposition can cause serious heavy metal(loids)pollution to the surrounding soil *** microorganisms adapt their metabolism to such conditions,driving alterations in soil *** study aims to elucidate the response patterns of nitrogen-cycling microorganisms under long-term heavy metal(loids)*** results showed that the diversity and abundance of nitrogen-cyclingmicroorganisms showed negative feedback to heavy metal(loids)*** microorganisms were shown to be the dominant microorganisms with over 60%of relative abundance and a complex community structure including 27 ***,the key bacterial species in the denitrification process were calculated using a random forest model,where the top three key species(Pseudomonas stutzei,Sphingobium japonicum and Leifsonia rubra)were found to play a prominent role in nitrite *** gene analysis and qPCR revealed that nirK,which is involved in nitrite reduction,significantly accumulated in the most metal-rich soil with the increase of absolute abundance of 63.86%.The experimental results confirmed that the activity of nitrite reductase(Nir)encoded by nirK in the soil was increased at high concentrations of heavy metal(loids).Partial least squares-path model identified three potential modes of nitrite reduction processes being stimulated by heavy metal(loids),the most prominent of which contributed to enhanced nirK abundance and soil Nir activity through positive stimulation of key *** results provide new insights and preliminary evidence on the stimulation of nitrite reduction processes by heavy metal(loids).

关键词： N cycle Nitrite reduction Nitrite reductase Metagenome Key species

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Nb-V microalloying on the hot deformation behavior of medium Mn steels

引用

International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 360-368页

作者： Yumeng Wang Qinyi Guo Bin hu Haiwen Luo School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China

The influence of Nb-V microalloying on the hot deformation behavior and microstructures of medium Mn steel(MMS)was investigated by uniaxial hot compression *** establishing the constitutive equations for simulating the measured flow curves,we successfully constructed deformation activation energy(Q)maps and processing maps for identifying the region of flow *** concluded the following consequences of Nb-V alloying for MMS.(i)The critical strain increases and the increment diminishes with the increasing deformation temperature,suggesting that NbC precipitates more efficiently retard dynamic recrystallization(DRX)in MMS compared with solute Nb.(ii)The deformation activation energy of MMS is significantly increased and even higher than that of some reported high Mn steels,suggesting that its ability to retard DRX is greater than that of the high Mn content.(iii)The hot workability of MMS is improved by narrowing the hot processing window for the unstable flow stress,in which fine recrystallized and coarse unrecrystallized grains are present.

关键词： medium manganese steel niobium-vanadium microalloying flow behavior dynamic recrystallization hot workability

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不同稀释方法对种公牛冷冻精液品质的影响

引用

中国奶牛 2009年第7期 21-23页

作者：胡月超辛英霞河北省畜牧良种工作站石家庄050061

研究选用18头荷斯坦种公牛,将采集合格的精液分为三份,用一步稀释法、两步快速稀释法和两步缓慢稀释法三种不同的稀释方法进行稀释,将稀释好的精液进行细管分装、平衡、冷冻,分别比较稀释前、稀释后和冷冻后精子的活率、畸形率和顶体完... 详细信息

研究选用18头荷斯坦种公牛,将采集合格的精液分为三份,用一步稀释法、两步快速稀释法和两步缓慢稀释法三种不同的稀释方法进行稀释,将稀释好的精液进行细管分装、平衡、冷冻,分别比较稀释前、稀释后和冷冻后精子的活率、畸形率和顶体完整率。结果表明,不同稀释方法对精子的稀释冲击不同,三种稀释方法中两步缓慢稀释法最好,一步稀释法对精子的顶体损伤最大。

关键词：稀释方法种公牛精液

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Development of a net ozone production rate detection system based on dual-channel cavity ring-down spectroscopy

引用

Journal of Environmental Sciences 2025年第3期149卷 419-430页

作者： Jinzhao Tong Renzhi hu Changjin hu Xiaoyan Liu Haotian Cai Chuan Lin Liujun Zhong JiaweiWang Pinhua Xie School of Environmental Science and Optoelectronic Technology University of Science and Technology of ChinaHefei 230026China Anhui Institute of Optics and Fine Mechanics Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China School of Pharmacy Anhui Medical UniversityHefei 230032China

A novel system for measuring net photochemical ozone production rates in the atmosphere based on cavity ring-down spectroscopy(OPR-CRDS)was *** system consists of two chambers(a reaction chamber and a reference chamber)and a dual-channel O_(x)-CRDS *** minimize the wall loss of O_(x)in the chambers,the inner surfaces of both chambers are coated with Teflon *** performance of the OPR-CRDS system was *** was found that even though the photolysis frequency(J value)decreased by 10%,the decrease in the P(O_(3))caused by the ultraviolet-blocking film coating was less than 3%.The two chambers had a good consistency in the mean residence time and the measurement of NO_(2)and O_(x)under the condition of no *** detection limit of the OPR-CRDS was determined to be 0.20 ppbv/*** further verify the accuracy of the system,the direct measurement values of the OPR-CRDS systemwere comparedwith the calculation results based on radical(OH,HO_(2),and RO_(2))reactions,and a good correlation was obtained between the measured and calculated ***,the developed instrument was applied to obtain the comprehensive field observations at an urban site in the Yangtze River Delta(China)for 40 days,the time series and change characteristics of the P(O_(3))were obtained directly,and the good environmental adaptability and stability of the OPR-CRDS system were *** is expected that the new instrument will be beneficial to investigations of the relationship between P(O_(3))and its precursors.

关键词： Ozone Net photochemical production rate CRDS Atmospheric chemistry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Accelerated warming of High Mountain Asia predicted at multiple years ahead

引用

Science Bulletin 2025年第3期70卷 419-428页

作者： Shuai hu Tianjun Zhou Bo Wu Key Laboratory of Earth System Numerical Modeling and Application Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China University of the Chinese Academy of Sciences Beijing 100049China

High-Mountain Asia(HMA)is an important source of freshwater since it holds the largest reservoir of frozen water outside the polar *** feeds ten great rivers,ultimately supporting more than 2 billion ***,the threat of accelerated glacier melt,which is a consequence of unprecedented global warming since the early 1950s,threatens water resources in the surrounding *** predictions of the near-term temperature change and synergistic mass loss of glaciers are essential but challenging to implement because of the impacts of internal climate ***,based on large ensembles of state-of-the-art decadal climate prediction experiments,we provide evidence that the internally generated surface air temperature variations in HMA can be predicted multiple years in advance,and the model initialization has robust added value to the decadal prediction ***-time decadal forecasts suggest that the HMA will experience accelerated warming in 2025-2032,where the surface warming will increase by 0.98°C(0.67 to 1.33°C;5%to 95%range)relative to the reference period 1991-2020,which is equivalent to 1.75 times the observed warming during *** decadal predictability originates from extratropical Pacific decadal variability modes,which modulate the convective heating in the tropical Pacific and influence HMA via the eastward-propagating atmospheric Kelvin *** warming in the coming decade will likely increase the shrinkage of the glacier volume over the HMA by 1.4%.This change poses unprecedented challenges,including potential water scarcity,ecosystem disruption,and increased risk of natural disasters,to HMA and surrounding regions.

关键词： Decadal prediction High Mountain Asia Surface air temperature Pacific decadal variabilities Glacier

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanical properties and flow stress constitutive relationship of Ti–6Al–4V alloy with equiaxed microstructure at cryogenic temperatures

引用

Chinese Journal of Aeronautics 2025年第1期38卷 365-379页

作者： Jingwen hu Xun CHEN Yashun WANG Chen YANG College of Intelligence Science and Technology National University of Defense TechnologyChangsha 410073China Laboratory of Science and Technology on Integrated Logistics Support National University of Defense TechnologyChangsha 410073China

This paper investigates the uniaxial tensile mechanical properties and flow behavior of Ti-6Al-4V alloys with equiaxed microstructure at cryogenic temperatures ranging from 77 K to298 K and strain rates from 10^(-4)/s to 10^(-2)/*** electron microscopy is utilized to analyze the fracture morphology,aiming to reveal the fracture behavior at various *** applicability of the Zener-Hollomon parameter and the Johnson-Cook model in describing the flow stress of Ti-6Al-4V at cryogenic temperatures is ***,a constitutive relationship modeling method based on the variational recurrent networks is *** test results show a significant increase in the strength of equiaxed Ti-6Al-4V alloy under cryogenic conditions while the plastic deformation process is ***,the fracture analysis indicates that even at 77 K,the fracture process is still dominated by ductile fracture,and brittle fracture does not occur within the range of 77 K to 298 *** fitting results validate the performance of the Zener-Hollomon parameter and the Johnson-Cook model in describing the deformation flow stress of Ti-6Al-4V alloy at cryogenic *** results also indicate that the proposed constitutive relationship modeling method based on the variational recurrent network performs better,making it a potential method for widespread applications.

关键词： Titanium alloys Mechanical properties Cryogenics Fracture testing Flow behavior

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

人冠状病毒NL63超速离心富集方法的建立及优化

引用

中华实验和临床病毒学杂志 2022年第2期36卷 199-204页

作者：胡月超宋敬东耿合员朱娜谭文杰中国疾病预防控制中心病毒病预防控制所国家卫生健康委医学病毒和病毒病重点实验室北京102206 中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室北京100052 中华人民共和国南京海关卫生检疫中心实验室南京210019

目的建立并优化人冠状病毒NL63参考株(HCoV-NL63-NC_005831)的体外富集方法。方法以恒河猴肾细胞系(LLC-MK2)培养HCoV-NL63,以离心力135000×g、分别离心病毒培养上清4 h、6 h、8 h、10 h和12 h以富集病毒,并以商品化PEG沉淀试剂盒富集... 详细信息

目的建立并优化人冠状病毒NL63参考株(HCoV-NL63-NC_005831)的体外富集方法。方法以恒河猴肾细胞系(LLC-MK2)培养HCoV-NL63,以离心力135000×g、分别离心病毒培养上清4 h、6 h、8 h、10 h和12 h以富集病毒,并以商品化PEG沉淀试剂盒富集方法作为对照。采用实时荧光定量PCR(qRT-PCR)、半数组织培养感染剂量(TCID50)和空斑实验对原始病毒液、富集病毒液分别进行病毒核酸和病毒生物活性定量检测,评价病毒富集效果;通过透射电镜负染观测病毒富集前后的形态。结果经层超速离心法及经PEG沉淀法分别进行体积100∶1富集处理,两种方法获得的富集病毒液相较于原液的病毒核酸浓度及活病毒浓度均有提高(P0.05)。结论超速离心法相较于商品化的PEG沉淀法能更有效富集HCoV-NL63病毒;离心力为135000×g时,选择8 h的超离时间可获得较优的体外富集效果。

关键词： HCoV-NL63 超速离心 PEG qRT-PCR 病毒富集

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：