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G2-LIKE CAROTENOID REGULATOR(SlGCR)is a positive regulator of lutein biosynthesis in tomato

aBIOTECH 2022年第4期3卷 267-280页

作者： Siyan Ren Yong Yuan hsihua wang Yang Zhang Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu610064China Sanya Institute of China Agricultural University Sanya572025China Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops College of HorticultureChina Agricultural UniversityBeijing100193China

Lutein is an oxygen-containing carotenoid synthesized in plant chloroplasts and *** plays an indispensable role in promoting plant growth and maintaining eye health in *** rate-limiting step of lutein biosynthesis is catalyzed by the lycopeneε-cyclase enzyme(LCYE).Although great progress has been made in the identification of transcription factors involved in the lutein biosynthetic pathway,many systematic molecular mechanisms remain to be ***,using co-expression analysis,we identified a gene,G2-LIKE CAROTENOID REGULATOR(SlGCR),encoding a GARP G2-like transcription factor,as the potential regulator of SlLCYE in *** of SlGCR reduced the expression of carotenoid biosynthetic genes and the accumulation of carotenoids in tomato *** contrast,overexpression of SlGCR in tomato fruit significantly increased the expression of relevant genes and enhanced the accumulation of *** can directly bind to the SlLCYE promoter and activate its *** addition,we also discovered that expression of SlGCR was negatively regulated by the master regulator SlRIN,thereby inhibiting lutein synthesis during tomato fruit *** together,we identified SlGCR as a novel regulator involved in tomato lutein biosynthesis,elucidated the regulatory mechanism,and provided a potential tool for tomato lutein metabolic engineering.

关键词： Solanum lycopersicum GARP G2-like transcription factor Lutein biosynthesis

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Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering

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Synthetic and Systems Biotechnology 2022年第4期7卷 1159-1166页

作者： Yanjun Wu Yong Yuan Wenqian Jiang Xin Zhang Siyan Ren hsihua wang Xiaolan Zhang Yang Zhang Key Laboratory of Bio-resource and Eco-environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu610064China Sanya Institute of China Agricultural University Sanya572025China Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops College of HorticultureChina Agricultural UniversityBeijing100193China

Carotenoids constitute a large group of natural pigments widely distributed in *** compounds not only provide fruits and flowers with distinctive colors,but also have significant health benefits for *** and zeaxanthin,both oxygen-containing carotenoids,are considered to play vital roles in promoting ocular development and maintaining eye ***,humans and mammals cannot synthesize these carotenoid derivatives,which can only be taken from certain fruits or ***,by introducing four endogenous synthetic genes,SlLCYE,SlLCYB,SlHYDB,and SlHYDE under fruit-specific promoters,we report the metabolic engineering of lutein/zeaxanthin biosynthesis in tomato *** lines overexpression of one(SlLCYE),two(SlLCYE and SlLCYB;SlLCYB and SlHYDB),and all these four synthetic genes re-established the lutein/zeaxanthin biosynthetic pathways in the ripe tomato fruit and thus resulted in various types of carotenoid riched *** analyses of these engineered tomato fruits showed the strategy involved expression of SlLCYE tends to produceα-carotene and lutein,as well as a higher content of β-carotene and zeaxanthin was detected in lines overexpressing *** addition,the different combinations of engineered tomatoes with riched carotenoids showed higher antioxidant capacity and were associated with a significantly extended shelf life during postharvest *** work provides a successful example of accurate metabolic engineering in tomato fruit,suggesting the potential utility for synthetic biology to improve agronomic traits in *** biofortified tomato fruits could be also exploited as new research subjects for studying the health benefits of carotenoid derivatives.

关键词： Solanum lycopersicum Tomato fruit Metabolic engineering Lutein Zeaxanthin Antioxidant capacity

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