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Phenotypic and phylogenetic characterization of Lactobacillus species isolated from traditional Lighvan cheese

Food Production, Processing and Nutrition 2021年第1期3卷 274-282页

作者： Haleh Forouhandeh Sepideh Zununi Vahed hossein ahangari Vahideh Tarhriz Mohammad Saeid Hejazi Molecular Medicine Research Center Biomedicine InstituteTabriz University of Medical SciencesTabrizIran Kidney Research Center Tabriz University of Medical SciencesTabrizIran Department of Food Science and Technology Faculty of Nutrition and Food SciencesTabriz University of Medical SciencesTabrizIran Department of Pharmaceutical Biotechnology Faculty of PharmacyTabriz University of Medical SciencesTabrizIran

Lighvan cheese(Lighvan panir)is among the most famous traditional cheese in Iran for its desired aroma and ***,the lactic acid bacteria especially the genus Lactobacillus are the critical factors in developing the aroma,flavor,and texture in Lighvan *** this study,the Lactobacillus population of the main Lighvan cheese was *** Lactobacillus of the main Lighvan cheese was isolated using specific culture methods according to previously published ***,the phylogenetic features were investigated and the phenotypic characteristics were examined using specific culture ***-eight Gram-positive bacterial species were identified belonged to the genus *** to the same sequences as each other,three groups(A,B,and C)of isolates were categorized with a high degree of similarity to ***(100%)and *** group(***,***,and ***)(99.0 to 100%).Random amplified polymorphic DNA(RAPD)fingerprint analysis manifested the presence of three clusters that were dominant in traditional Lighvan ***Іwas divided into 4 *** the result of carbohydrate fermentation pattern and 16S rRNA sequencing,isolates were identified as *** isolates in clusters II and III represented *** and ***,respectively as they were identified by 16S rRNA sequencing and fermented carbohydrate *** result indicated that the specific aroma and flavor of traditional Lighvan cheese can be related to its Lactobacillus population including ***,***,***,and ***.

关键词： Lighvan cheese Lactobacillus 16S rRNA RAPD fingerprints

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