Background. To understand interactions between dendritic cells (DCs) and virus es, in vitro-cultured monocyte-derived DCs are usually used for functional ana lyses. However, several recent studies indicate that circul...
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Background. To understand interactions between dendritic cells (DCs) and virus es, in vitro-cultured monocyte-derived DCs are usually used for functional ana lyses. However, several recent studies indicate that circulating blood DCs are d ifferent from monocyte-derived DCs, both phenotypically and functionally. Indee d, circulating DCs act as functional antigen-presenting cells in vivo. This stu dy was conducted to evaluate the function of circulating blood DCs in patients w ith chronic hepatitis C and to examine whether circulating DCs from these patien ts were infected by hepatitis C virus (HCV). Methods. The phenotypes and biologi cal functions of circulating DCs from patients with chronic hepatitis C (n = 27) , patients with non-HCV chronic liver disease (n = 7), and normal volunteers (n = 13) were analyzed. The presence of the HCV genome sequence in circulating blo od IDS and in subsets of circulating IDS (myeloid DCs and plasmacytoid DCs) in p atients with chronic hepatitis C was assessed. Results. The stimulatory capacity of circulating DCs was significantly reduced in patients with chronic hepatitis C compared to patients with non-HCV chronic liver diseases and normal controls (P nA was identified in the overall population of circulating DCs , and in myeloid DCs and plasmacytoid DCs. nucleotide sequences of the 5 non-co ding region of HCV RnA showed marked differences between paired samples of circu lating DCs and sera from the same patients. Conclusions. Our results indicate th e dysfunction and infection of circulatory blood DCs in chronic HCV infection. T his may compromise the capacity of patients with hepatitis C to induce an effect ive antiviral immune response.
Recent progress in the physics and engineering design study for themodification programme of JT-60 is presented. In order to achieve a steady state high-βplasmaoperation, which is the dominant issue of this programme...
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Recent progress in the physics and engineering design study for themodification programme of JT-60 is presented. In order to achieve a steady state high-βplasmaoperation, which is the dominant issue of this programme, physics design for the MHD control and thestability analysis is investigated. Engineering design and the R & D for the superconducting coils,irradiation shield are performed well towards the mission of programme.
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