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Excitatory effect of histamine on neuronal activity of rat cerebellar fastigial nucleus in vitro

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Chinese Science Bulletin 2007年第4期52卷 497-503页

作者： TANG Biao ZHANG Jun li hongzhao ZHU JingNing WANG JianJun Department of Biological Science and Technology/State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China Jiangsu Key Laboratory for Bioresource Technology School of Life Sciences Nanjing Normal University Nanjing 210097 China

The cerebellar fastigial nucleus (FN) holds an important role in motor control and body balance. Previous studies have revealed that the nucleus is innervated by direct hypothalamocerebellar histaminergic fibers. However, the functional role of histaminergic projection in cerebellar FN has never been established. In this study, we investigated the effect of histamine on neuronal firing of cerebellar FN by using slice preparations. Sixty-five FN cells were recorded from 47 cerebellar slices, and a vast majority of the cells responded to histamine stimulation with an excitatory response (58/65, 89.2%). Perfusing slices with low-Ca2+/high-Mg2+ medium did not block the histamine-induced excitation (n=10), supporting a direct postsynaptic action of histamine on the cells. Furthermore, the excitatory effect of histamine on FN neurons was not blocked by selective histamine H1 receptor antagonist triprolidine (n=15) or chlorpheniramine (n=10), but was effectively suppressed by ranitidine (n=15), a highly selective histamine H2 receptor antagonist. On the other hand, highly selective histamine H2 receptor agonist dimaprit (n=20) instead of histamine H1 receptor agonist 2-pyridylethylamine (n=16) mimicked the ex- citatory effect of histamine on FN neurons. The dimaprit-induced FN neuronal excitation was effectively antagonized by selective histamine H2 receptor antagonist ranitidine (n=13) but not influenced by se- lective histamine H1 receptor antagonist triprolidine (n=15). These results demonstrate that histamine excites cerebellar FN cells via the histamine H2 receptor mechanism and suggest that the hypotha- lamocerebellar histaminergic fibers may modulate cerebellar FN-mediated sensorimotor integration through their excitatory innervations on FN neurons.

关键词：大鼠小脑顶核神经活动组胺受体兴奋作用

Impact of thermal processes on multi-crystalline silicon

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Frontiers in Energy 2017年第1期11卷 32-41页

作者： Moonyong KIM Phillip HAMER hongzhao li David PAYNE Stuart WENHAM Malcolm ABBOTT Brett HALLAM School of Photovoltaic and Renewable Energy Engineering Universityof New South Wales Sydney NSW 2052 Australia Department of Materials University of Oxford Oxford OX1 3PH United Kingdom

Fabrication of modem multi-crystalline silicon solar cells involves multiple processes that are thermally intensive. These include emitter diffusion, thermal oxida- tion and firing of the metal contacts. This paper illustrates the variation and potential effects upon recombination in the wafers due to these thermal processes. The use of light emitter diffusions more compatible with selective emitter designs had a more detrimental effect on the bulk lifetime of the silicon than that of heavier diffusions compatible with a homogenous emitter design and screen-printed contacts. This was primarily due to a reduced effectiveness of gettering for the light emitter. This reduction in lifetime could be mitigated through the use of a dedicated gettering process applied before emitter diffusion. Thermal oxida- tions could greatly improve surface passivation in the intra- grain regions, with the higher temperatures yielding the highest quality surface passivation. However, the higher temperatures also led to an increase in bulk recombination either due to a reduced effectiveness of gettering, or due to the presence of a thicker oxide layer, which may interrupt hydrogen passivation. The effects of fast firing were separated into thermal effects and hydrogenation effects. While hydrogen can passivate defects hence improving the performance, thermal effects during fast firing can dissolve precipitating impurities such as iron or de-getter impurities hence lower the performance, leading to a poisoning of the intra-grain regions.

关键词： gettering grain boundaries hydrogen impurities oxidation passivation solar cell

Modulation of neuronal activity of cerebellar fastigial nucleus by locus coeruleus stimulation in the rat

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Chinese Science Bulletin 1998年第11期43卷 940-944页

作者： Wang Tao Wang Jianjun Cheng Hong li hongzhao Yu Qixiang Nanjing Univ Dept Biol Sci & Technol Nanjing 210093 Peoples R China

The effects of stimulating locus coeruleus (LC) on neuronal activity of cerebellar fastigial nucleus (FN) was investigated. Stimulation of LC elicited inhibitory, excitatory and biphasic (inhibition-excitation) responses from FN cells. The majority of responsive cells showed an inhibitory response with a latency of less than 10 ms. Injection of α adrenoreceptor antagonists phentolamine (ⅳ) could block the inhibitory response of FN cells to the LC stimulation, but propranolol (ⅳ), a β adrenoreceptor antagonist, could not. These results suggest that LC-cerebellar noradrenergic afferent fibers may be involved in the cerebellar sensorimotor integration process by exerting their modulatory action on the cerebellar nuclear cells’ activities.

关键词： locus coeruleus cerebellar fastigial nucleus coeruleo-cerebellar pathway noradrenergic fibers.

Type 1 T-cell responses in chlamydial lung infections are associated with local MIP-1a response

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Cellular & Molecular Immunology 2010年第5期7卷 355-360页

作者： Naihong Zhang Zhaoe Wang Xiaofei Tang Haiping Wang hongzhao li Huanjun Huang Hong Bai Xi Yang Department of Immunology Tianjin Medical UniversityTianjin Key Laboratory of Cellular and Molecular ImmunologyKey Laboratory of Educational Ministry of ChinaTianjinChina Laboratory for Infection and Immunity Departments of Medical Microbiology and ImmunologyFaculty of MedicineUniversity of ManitobaWinnipegManitobaCanada

Chemokines and their receptors are important mediators of leukocyte trafficking and recruitment and sometimes work as modulators of T-cell responses during infections and *** the biological activity of chemokines has been found to influence the course of ***,little is known about the role of chemokine responses during chlamydial lung *** therefore analyzed the dynamics of multiple chemokines,which are frequently associated with type 1(Th1)T cell immune responses,and their receptors for their expression in the lungs during Chlamydia muridarum(Cm)*** also examined the relationship between chemokine responses and the development of Th1 responses as well as the clearance of *** results showed that in parallel with the high levels of gamma interferon(IFN-c)and IL-12 production in the lungs and draining lymph nodes,and the expansion of IFN-c-producing CD4 and CD81 T cells,the production of the cell-related chemokines RANTES,IFN-c-inducible protein-10(IP-10)and macrophage inflammatory protein-1a(MIP-1a)and their receptor CCR1 was elevated in the lung tissues after ***,in a later phase of infection,the expression of RANTES and IP-10 remained elevated but the expression of MIP-1a and CCR1 decreased to a low level,which suggests a closer association with the pattern of Th1 cytokine responses in the process of *** results suggest a close association between the MIP-1a response and the Th1-type T-cell responses in chlamydial lung infections .

关键词： Chlamydial pneumonia MIP-1a RANTES Th1 response

基于CRISPR/Cas系统的病原核酸检测技术研究进展

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浙江大学学报(农业与生命科学版) 2023年第5期49卷 618-632页

作者：李红招王浩尹睿乐敏李艳浙江大学海南研究院浙江大学动物科学学院动物预防医学研究所/浙江省动物预防医学重点实验室

作为一种古老的细菌和古菌免疫系统，规律成簇的间隔短回文重复序列及其相关蛋白[clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas), CRISPR/Cas]系统现已发展为新兴的热门基因编辑工具，极大地推动了其他多个生物学相关领域的发展。其中，通过结合CRISPR/Cas系统与核酸恒温扩增技术建立了一些新型的高效、灵敏、不依赖仪器设备的检测方法，如DNA内切酶靶向的CRISPR反式报告系统（DNA endonuclease-targeted CRISPR trans-reporter, DETECTR）、特异性高灵敏度的酶报告器系统（specific high-sensitivity enzymatic reporter unlocking, SHERLOCK）等，不但提高了CRISPR/Cas系统在不同应用场景下的检测性能，更进一步激发了其在现场检测中的应用潜力。本文基于近年来被广泛应用的3种CRISPR/Cas系统（CRISPR/Cas9、CRISPR/Cas12a以及CRISPR/Cas13）的生物核酸检测方法，阐述了CRISPR/Cas系统的生物学意义及一般作用原理，并通过回顾以往开展的CRISPR/Cas系统相关的病原检测研究，比较分析了不同检测系统的特点以及在实际应用过程中可能存在的不足，为针对不同病原在不同应用场景下建立更加高效、合理的CRISPR/Cas系统检测方法提供了有益参考。

关键词： CRISPR/Cas系统 Cas蛋白病原核酸检测方法

来源：博看期刊

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远程外科面临的机遇与挑战

中华外科杂志 2024年第1期62卷 27-30页

作者：张旭李宏召王野解放军总医院泌尿外科医学部北京100853

远程外科是一种利用远程通信技术和机器人手术系统进行手术的新兴方式,打破了空间对外科手术开展的束缚,是未来外科学的重要发展方向。中国幅员辽阔,远程外科有望成为改善区域医疗资源分布不均衡的候选方法。近年来,随着国产机器人手术... 详细信息

远程外科是一种利用远程通信技术和机器人手术系统进行手术的新兴方式,打破了空间对外科手术开展的束缚,是未来外科学的重要发展方向。中国幅员辽阔,远程外科有望成为改善区域医疗资源分布不均衡的候选方法。近年来,随着国产机器人手术系统和通信技术的发展与成熟,我国在远程外科领域取得了快速进步,但仍处于发展的初级阶段,面临机遇的同时将迎接诸多挑战。未来20年将是世界远程外科发展的黄金机遇期,相信我国外科学家一定会抓住机遇、迎接挑战,引领远程外科发展。

关键词：外科手术远程医学远程外科机器人手术系统机遇挑战

当归六黄汤加减联合化痰祛瘀调瘿方对甲状腺功能亢进症患者TR-Ab、TPOAb表达的影响

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辽宁中医杂志 2022年第4期49卷 55-59页

作者：李红照济南市中医医院山东济南250000

目的研究当归六黄汤加减联合化痰祛瘀调瘿方对甲状腺功能亢进症患者促甲状腺激素受体抗体(TR-Ab)、甲状腺过氧化物酶抗体(TPOAb)表达的影响。方法选取2019年1月—2021年1月在医院就诊的甲状腺功能亢进症患者148例,根据治疗方法的不同分... 详细信息

目的研究当归六黄汤加减联合化痰祛瘀调瘿方对甲状腺功能亢进症患者促甲状腺激素受体抗体(TR-Ab)、甲状腺过氧化物酶抗体(TPOAb)表达的影响。方法选取2019年1月—2021年1月在医院就诊的甲状腺功能亢进症患者148例,根据治疗方法的不同分为当归六黄汤组和联合组,各74例。当归六黄汤组给予当归六黄汤加减治疗,联合组在当归六黄汤组用药的基础上给予化痰祛瘀调瘿方治疗,两组均持续治疗8周。检测患者在治疗前及治疗4周、8周后TR-Ab、TPOAb表达水平。结果两组治疗后4周、8周TR-Ab、TPOAb、T_(3)、T_(4)、FT_(3)、FT_(4)、Vm水平及甲状腺肿大、突眼、心悸心慌、多食易饥评分低于两组治疗前,RI、PI水平高于两组治疗前(P<0.05)。联合组治疗后4周、8周TR-Ab、TPOAb、T_(3)、T_(4)、FT_(3)、FT_(4)、Vm水平及甲状腺肿大、突眼、心悸心慌、多食易饥评分低于当归六黄汤组4周、8周,RI、PI水平高于当归六黄汤组4周、8周(P<0.05)。与当归六黄汤组相比,联合组治疗总有效率升高(P<0.05)。结论当归六黄汤加减联合化痰祛瘀调瘿方能够改善甲状腺功能亢进症患者TR-Ab、TPOAb表达水平,缓解患者临床症状,疗效显著。

关键词：当归六黄汤加减化痰祛瘀调瘿方甲状腺功能亢进症促甲状腺激素受体抗体甲状腺过氧化物酶抗体

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高导热金刚石/铜复合材料的导热研究进展

稀有金属材料与工程 2020年第12期49卷 4146-4158页

作者：陈明和李宏钊王长瑞王宁李治佑唐丽娜南京航空航天大学直升机传动技术国家重点实验室江苏南京210016 上海航天设备制造总厂有限公司上海200245

金刚石/铜复合材料具有密度低、热导率高及热膨胀系数可调等优点,且与新一代芯片具有良好的热匹配性能,在高热流密度电子封装领域具有非常广泛的应用前景。然而由于金刚石与铜界面润湿性差,界面热阻高,导致材料热导率比铜还低,限制了其... 详细信息

金刚石/铜复合材料具有密度低、热导率高及热膨胀系数可调等优点,且与新一代芯片具有良好的热匹配性能,在高热流密度电子封装领域具有非常广泛的应用前景。然而由于金刚石与铜界面润湿性差,界面热阻高,导致材料热导率比铜还低,限制了其应用。为了改善其界面润湿性,通过在金刚石表面金属化或对铜基体预合金化等手段来修饰复合材料界面,以提高金刚石/铜复合材料的热导率。本文综述了表面改性、导热模型相关的界面理论以及有限元模拟的研究进展,讨论了制备工艺、导热模型和未来发展的关键方向,总结了金刚石添加量、颗粒尺寸等制备参数对其微观组织结构和导热性能的影响规律。

关键词：金刚石/铜复合材料界面润湿性界面理论有限元仿真

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试论小学数学教学中学生数学思维能力的培养

家长 2020年第25期 87-88页

作者：李宏照甘肃省庆阳市合水县西华池初级中学(乐蟠小学)

随着我国九年义务教育体系的改革,数学教学的方法也越来越多样化,小学数学的课程体系也越来越完善。根据我国目前的教育情况来看,成绩已经不能代表学生的一切了。本文针对小学数学教学中学生数学思维能力的培养问题进行研究和讨论。

关键词：小学数学思维能力培养方法