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The Crop Journal 2024年第3期12卷 676-687页

作者： Chuan Li Yun Bai Yuwei Hou Siyu Wang Yue Xin Tao Yu Jing Wang Xiaowei Liu hongyang yi Chao Xia Hai Lan Moju Cao State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Maize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Jilin Engineering Research Center for Crop Biotechnology Breeding College of Plant ScienceJilin UniversityChangchun 130012JilinChina

Maize plant architecture influences planting density and,in turn,grain *** of the plant architecture-related traits can be described as organ *** describe a miniature maize mutant,Tiny plant 4(Tip4),which exhibits reduced size of multiple organs and exhibits a semi-dominant monofactorial inheritance *** cloning confirmed that a 4-bp deletion in the NAC TF with transmembrane motif 1-Like(NTL)gene ZmNTL2,denoted as ZmNTL2^(Δ),confers the Tip4 ***-PCR showed that ZmNTL2 was expressed in all tested ***2 functions as a transcriptional activator and is located in both the nucleus and *** mutation does not affect the mRNA abundance of ZmNTL2 locus,but it does result in the loss of transmembrane domain and confines the ZmNTL2^(Δ)protein to the *** out ZmNTL2 has no effect on maize organ size development,indicating that the 4-bp deletion might be a gain-of-function mutation in organ size *** transcriptome sequencing with cytokinin and auxin content determination suggests that the decreased organ size may be possibly mediated by changes in hormone homeostasis.

关键词： Organ size c-ray mutagenesis Membrane-bound NAC TF Hormone homeostasis Maize

一份新的玉米永久性失绿突变体chs10的鉴定及基因克隆

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四川农业大学学报 2024年第1期42卷 46-56,102页

作者：侯雨微岳毓菁李川苏帅易洪杨曹墨菊四川农业大学玉米研究所/农业农村部西南玉米生物学与遗传育种重点实验室成都611130

【目的】利用60Co-γ射线处理自交系齐319获得了一份新的玉米叶色突变体,对该突变体进行遗传分析、基因定位与克隆,并对候选基因的功能进行初步分析。【方法】以该突变体为亲本,构建遗传分析群体和基因定位群体;通过图位克隆技术获得关... 详细信息

【目的】利用60Co-γ射线处理自交系齐319获得了一份新的玉米叶色突变体,对该突变体进行遗传分析、基因定位与克隆,并对候选基因的功能进行初步分析。【方法】以该突变体为亲本,构建遗传分析群体和基因定位群体;通过图位克隆技术获得关键候选基因;利用生物信息学方法分析关键候选基因的结构和进化关系;通过qRT-PCR技术检测候选基因在不同组织中的表达差异;同时运用烟草瞬时表达技术对候选基因进行亚细胞定位表达分析。【结果】鉴定了一份玉米永久性失绿突变体chs10(Permanent chlorosis 10),chs10自V2时期开始,叶片从幼苗基部到顶部逐渐由绿转黄,后期的生长发育过程中不再复绿,并且,整个植株包括叶鞘、叶环、茎秆、苞叶和雄穗也均为黄色。该突变体受一对隐性核基因控制,可稳定遗传,植株生长发育正常,可正常授粉结实。突变基因被定位于玉米第10染色体长臂标记SNP-2和SNP-3之间约0.17 Mb范围内,确定了关键候选基因Zm00001d025860,qRT-PCR结果显示Zm00001d025860在玉米根、茎、叶和叶鞘中均有表达,但在叶片中高表达,亚细胞定位结果显示目标蛋白被定位在细胞膜和叶绿体中。利用STRING预测发现Zm00001d025860与卟啉结合蛋白GUN4基因互作,GUN4与叶绿素合成中的镁离子螯合酶(MgCh)具有反馈调节作用。【结论】Zm00001d025860基因的突变导致突变体chs10叶色的改变,并且Zm00001d025860可能通过参与叶绿素合成途径来调控叶色变化。突变体chs10的发现一方面丰富了玉米叶色突变体研究的基因资源,同时为解析镁离子螯合酶在叶绿素合成途径中的作用机理奠定了材料基础。

关键词：玉米遗传分析叶色突变体 chs10 图位克隆

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新型冠状病毒的演化与临床特征

国际流行病学传染病学杂志 2024年第3期51卷 154-163页

作者：杨素梅廖忠惠崔妍欣仪洪洋卢洪洲蚌埠医科大学公共卫生学院蚌埠233030 深圳市第三人民医院国家感染性疾病临床医学研究中心肝病研究所深圳518112

SARS-CoV-2以其严重的致病性、快速的传播速率以及高频的突变率给全球带来了严重的灾难。自SARS-CoV-2暴发以来, 在各种自然环境、治疗药物以及疫苗的选择压力下, 出现多种变异株, 其在复制、传播、致病性、免疫逃逸以及诱发长新冠的能... 详细信息

SARS-CoV-2以其严重的致病性、快速的传播速率以及高频的突变率给全球带来了严重的灾难。自SARS-CoV-2暴发以来, 在各种自然环境、治疗药物以及疫苗的选择压力下, 出现多种变异株, 其在复制、传播、致病性、免疫逃逸以及诱发长新冠的能力上都有着不同的表现, 增加了COVID-19疫情防控的不确定因素。本文对多种SARS-CoV-2变异株(Alpha毒株、Beta毒株、Gamma毒株、Delta毒株以及Omicron毒株)的演化过程与临床特征进行总结, 为SARS-CoV-2的防控与治疗提供理论支持。

关键词：新型冠状病毒演化临床特征长新冠

A valine residue deletion in ZmSig2A, a sigma factor, accounts for a revertible leaf-color mutation in maize

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The Crop Journal 2021年第6期9卷 1330-1343页

作者： Chuan Li Jingwen Wang Zhaoyong Hu Yuanyan Xia Qiang Huang Tao Yu hongyang yi Yanli Lu Jing Wang Moju Cao Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130SichuanChina

A nuclear-encoded sigma(σ) factor is essential for the transcriptional regulation of plant chloroplastencoded genes. Five putative maize r factors have been identified by database searches, but their functions are unknown. We report a maize leaf color mutant etiolated/albino leaf 1(eal1) that was derived from space mutation breeding. The eal1 mutant displays etiolated or albino leaves that then gradually turn to normal green at the seedling stage. The changes in eal1 leaf color are associated with changes in photosynthetic pigment content and chloroplast development. Map-based cloning revealed that a single amino-acid deletion changing Val_(480)-Val_(481)-Val_(482) to Val_(480)-Val_(481), in the C-terminal domain σ_(4) of the putative σ factor ZmSig2A, is responsible for the eal1 mutation. In comparison with the expression level of the wild-type(WT) allele ZmSig2A^(+) in WT plants, much higher expression of the mutant allele ZmSig2A^(⊿V) in eal1 plants was detected before the eal1 plants turned to normal green. ZmSig2A shows the highest similarity to rice OsSig2A and Arabidopsis SIG2. Ectopic expression of ZmSig2A^(+) or ZmSig2A^(⊿V) driven by the cauliflower mosaic virus 35 S promoter rescued the pale green leaf of the sig2 mutant, but ectopic expression of ZmSig2A^(⊿V) driven by the SIG2 promoter did not. We propose that the Val deletion generated a new weak allele of ZmSig2A that cannot completely abolish the ZmSig2A function. Some genes involved in chloroplast development and photosynthesis-associated nuclear genes showed significant expression differences between eal1 and WT plants. We conclude that ZmSig2A encoding a r factor is essential for maize chloroplast development. The eal1 mutant with a weak allele of ZmSig2A represents a valuable genetic resource for investigating the regulation of ZmSig2A-mediated chloroplast development in *** eal1 mutation may be useful as a marker for early identification and elimination of false hybrids or transgene transm

关键词： Leaf color mutant Map-based cloning Sigma factor Chloroplast development Maize

Multi-omics integration reveals potential stage-specific druggable targets in T-cell acute lymphoblastic leukemia

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Genes & Diseases 2024年第5期11卷 374-389页

作者： Zijun Yan Jie Xia Ziyang Cao hongyang Zhang Jinxia Wang Tienan Feng yi Shu Lin Zou Clinical Research Unit Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200062China Institute of Pediatric Infection Immunityand Critical Care MedicineShanghai Jiao Tong University School of MedicineShanghai 200062China Center for Clinical Molecular Laboratory Medicine of Children’s Hospital of Chongqing Medical University Chongqing 400014China Clinical Research Institute Shanghai Jiao Tong University School of MedicineShanghai 200025China Bioinformatics and BioMedical Bigdata Mining Laboratory School of Big HealthGuizhou Medical UniversityGuiyangGuizhou 554300China

T-cell acute lymphoblastic leukemia(T-ALL),a heterogeneous hematological malignancy,is caused by the developmental arrest of normal T-cell *** development of targeted therapeutic regimens is impeded by poor knowledge of the stage-specific aberrances in this *** this study,we performed multi-omics integration analysis,which included mRNA expression,chromatin accessibility,and gene-dependency database analyses,to identify potential stage-specific druggable targets and repositioned drugs for this *** multi-omics integration helped identify 29 potential pathological genes for *** genes exhibited tissue-specific expression profiles and were enriched in the cell cycle,hematopoietic stem cell differentiation,and the AMPK signaling *** these,four known druggable targets(CDK6,TUBA1A,TUBB,and TYMS)showed dysregulated and stage-specific expression in malignant T cells and may serve as stage-specific targets in *** TUBA1A expression level was higher in the early T cell precursor(ETP)-ALL cells,while TUBB and TYMS were mainly highly expressed in malignant T cells arrested at the CD4 and CD8 double-positive or single-positive ***6 exhibited a U-shaped expression pattern in malignant T cells along the naıve to maturation ***,mebendazole and gemcitabine,which target TUBA1A and TYMS,respectively,exerted stage-specific inhibitory effects on T-ALL cell lines,indicating their potential stage-specific antileukemic role in ***,our findings might aid in identifying potential stage-specific druggable targets and are promising for achieving more precise therapeutic strategies for T-ALL.

关键词： Multi-omics Stage-specific druggable targets Targeted therapeutic strategies T-cell acute lymphoblastic leukemia Drug repositioning

ZmMS39 encodes a callose synthase essential for male fertility in maize(Zea mays L.)

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The Crop Journal 2023年第2期11卷 394-404页

作者： Qunkai Niu Ziwen Shi Peng Zhang Shuai Su Bin Jiang Xiaowei Liu Zhuofan Zhao Suzhi Zhang Qiang Huang Chuan Li Tao Yu hongyang yi Tingzhao Rong Moju Cao Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Maize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Chengdu Agricultural College Chengdu 611130SichuanChina Sichuan Institute of Atomic Energy Chengdu 611130SichuanChina

Callose contributes to many biological processes of higher plants including pollen development,cell plate and vascular tissue formation,as well as regulating the transport function of *** functions of callose synthase genes in maize have been little *** describe a maize male-sterile mutant 39(ms39)characterized by reduced plant *** this study,we confirmed using CRISPR/Cas9 technology that a mutation in Zm00001d043909(ZmCals12),encoding a callose synthase,is responsible for the male sterility of the ms39 *** with male-fertile plants,callose deposition around the dyads and tetrads in ms39 anthers was significantly *** cell autophagy observed in ms39 anthers may have been due to the premature programmed cell death of tapetal cells,leading to collapse of the anther wall *** glucose metabolism in ms39 may have intensified autophagy in *** of the ms39 gene on maize heterosis by paired-crossed experiment with 11 maize inbred lines indicated that ms39 can be used for maize hybrid seed production.

关键词： Maize Genic male sterility Callose synthase Tapetal PCD Anther and pollen development Autophagy

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加洛林王朝军备考略

苏州科技大学学报（社会科学版） 2019年第6期36卷 75-82,108页

作者：易弘扬中国国家图书馆古籍馆

加洛林王朝是墨洛温王朝之后兴盛于8世纪中叶的法兰克王朝。虽然其国祚不长,“其兴也勃焉,其亡也忽焉”,经三代国主就被一分为三,但加洛林王朝的建立对日后乃至今天的欧洲格局产生深远的影响。加洛林王朝以武治国、幅员辽阔,诞生了杰出... 详细信息

加洛林王朝是墨洛温王朝之后兴盛于8世纪中叶的法兰克王朝。虽然其国祚不长,“其兴也勃焉,其亡也忽焉”,经三代国主就被一分为三,但加洛林王朝的建立对日后乃至今天的欧洲格局产生深远的影响。加洛林王朝以武治国、幅员辽阔,诞生了杰出的政治家、军事家查理曼大帝。其治下的法兰克军队,职业兵、征召兵、盟国军队协同作战,海陆军装备精良,还有当时先进的战略战术和训练手段,这些都为王朝南征北讨、开疆拓土提供了坚实的基础。

关键词：加洛林王朝法兰克军事查理曼大帝

玉米新选细胞质雄性不育系小孢子发育的细胞学观察及DNA甲基化分析

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遗传 2014年第10期36卷 1021-1026页

作者：张艳花易洪杨房明荣廷昭曹墨菊四川农业大学玉米研究所、教育部作物基因资源与遗传改良重点实验室、农业部西南玉米生物学及遗传育种重点实验室成都611130

细胞质雄性不育在高等植物中普遍存在,是杂种优势利用的重要工具,为推动植物杂种优势的利用发挥了重要作用。文章以本课题组前期新选的玉米细胞质雄性不育系A1、A2及保持系18(红)为材料,利用石蜡切片技术对不育材料小孢子发育过程进行... 详细信息

细胞质雄性不育在高等植物中普遍存在,是杂种优势利用的重要工具,为推动植物杂种优势的利用发挥了重要作用。文章以本课题组前期新选的玉米细胞质雄性不育系A1、A2及保持系18(红)为材料,利用石蜡切片技术对不育材料小孢子发育过程进行细胞学观察,采用高效液相色谱法(HPLC)对不同发育时期的叶片及不同发育时期的雄穗DNA进行甲基化分析,从细胞学和表观遗传学角度了解不育系A1、A2的败育机制。结果表明:不育材料A1、A2小孢子发生败育的主要时期为四分体时期至单核小孢子中期。在不育系A2中还存在另一种败育方式,即在花粉母细胞时期表现出败育特征。甲基化分析结果表明,保持系18(红)的叶片DNA甲基化水平从苗期到拔节期迅速上升,而不育系A1、A2叶片DNA甲基化水平基本保持不变;保持系雄穗DNA甲基化水平表现为从花粉母细胞时期到双核期逐渐升高,而不育材料A1、A2从花粉母细胞时期到双核期的雄穗DNA甲基化水平表现为先上升后下降的趋势,达到最高峰的时期均出现在小孢子发育的四分体时期。从小孢子发育的细胞学观察结果可以发现,小孢子败育的主要时期往往具有较高的甲基化水平,推测DNA甲基化水平变化可能与不育材料A1、A2的花粉败育有关。

关键词：玉米细胞质雄性不育细胞学观察 DNA甲基化

16世纪中西方火器的特点及《神器谱》对欧洲火器的改良

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江苏科技大学学报（社会科学版） 2019年第3期19卷 10-17,24页

作者：易弘扬白鸿叶国家图书馆古籍馆

明代著名科学家、火器专家赵士祯所著《神器谱》是明代研究火器的一部专著,其中既有其原创的火器,也有对西人火器的改造。火器发展到16世纪,明代的火器因其性能的缺点逐渐被性能更加优越的西方火器超越。同样也是在16世纪,更为先进的西... 详细信息

明代著名科学家、火器专家赵士祯所著《神器谱》是明代研究火器的一部专著,其中既有其原创的火器,也有对西人火器的改造。火器发展到16世纪,明代的火器因其性能的缺点逐渐被性能更加优越的西方火器超越。同样也是在16世纪,更为先进的西方火器经葡萄牙商人之手传入中国。在接触这些火器之后,赵士祯对其进行了改进,提升了性能,并在其基础上发明了多种原创火器。在改进和发明火器的基础上,他又进一步提出了相对应的军事思想,从器物和军事思想两个方面对明代中后期军事产生了重要的影响。

关键词：《神器谱》赵士祯火器明代欧洲

基于计算实验的PPP项目多主体协同合作行为激励仿真研究

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技术与创新管理 2022年第4期43卷 441-452页

作者：张云华伊弘阳昆明理工大学建筑工程学院云南昆明650500

PPP项目多主体合作行为选择具有复杂性和动态性,传统单一的激励机制和数理模型不能完全反映现实情景。为此,本研究基于PPP项目政企双方Agent属性和合作过程动态情景,构建计算实验仿真平台,从强化政企双方协同合作视角,建立以互惠偏好为... 详细信息

PPP项目多主体合作行为选择具有复杂性和动态性,传统单一的激励机制和数理模型不能完全反映现实情景。为此,本研究基于PPP项目政企双方Agent属性和合作过程动态情景,构建计算实验仿真平台,从强化政企双方协同合作视角,建立以互惠偏好为驱动的PPP项目多阶段、多主体计算实验激励仿真模型,并在不同激励条件下反复实验。根据实验结果得到如下结论:单一化的激励报酬在PPP项目中很难取得良好的激励效果;由于互惠偏好这一社会心理的普遍存在,致使随着激励报酬的提高,虽然PPP项目的整体协作水平能够得以大幅提高,但高额的激励成本会损害政府方的合理收益,因此,政府方制定的激励机制应平衡各方收益与成本的交互影响,以最优化的激励成本达到最佳的激励效果。

关键词： PPP项目协同合作计算实验