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Enhanced straw fermentation process based on microbial electrolysis cell coupled anaerobic digestion

Chinese Journal of Chemical Engineering 2022年第4期35卷 239-245页

作者： Xinyu Yan Bobo Wang Hongxia Liang Jie Yang Jie Zhao Fabrice Ndayisenga hongxun zhang Zhisheng Yu Zhi Qian College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China Research Center for Eco-Environmental Sciences-Institute of Microbiology Chinese Academy of Sciences-University of Chinese Academy of Sciences(RCEES-IMCAS-UCAS)Joint-Lab of Microbial Technology for Environmental ScienceBeijing 100085China Received:2020-11-12 Revised:2021-05-08 Online:2022-04-28 Published:2022-06-18

The low quality and yield of methane severely hinder the industrial application of straw biogas fermentation, and no effective solution has been found so far. In this study, a novel method was developed when a microbial electrolysis cell(MEC) was coupled with normal anaerobic fermentation to enhance methane yield and purity. The fermentation process achieved a methane purity of more than 85%, which is considerably higher than that of previously published reports. With microbial stimulation and an electric current, the degradation of fibers has been greatly enhanced. The MEC system substantially improved the yield and purity of biogas, bringing a new path to the synthesis of methane by carbon dioxide and hydrogen ions in solution under electron irradiation. Electrochemical index analysis showed extra methane synthesis, due to the external circuit electron transfer. The results of the gas chromatography and solid degradation rate showed that the carbon source of extra methane was CO_(2) produced during normal fermentation and additional volatile solid degradation. These results show that the MEC considerably enhanced the quality and yield of methane in the straw fermentation process, providing insights into normal anaerobic fermentation.

关键词： Microbial electrolysis cell(MEC) Methane Straw Fermentation Bioenergy

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Molecular characterization of microbial communities in bioaerosols of a coal mine by 454 pyrosequencing and real-time PCR

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Journal of Environmental Sciences 2015年第4期27卷 241-251页

作者： Min Wei Zhisheng Yu hongxun zhang College of Resources and Environment University of Chinese Academy of Sciences

Microbial diversity and abundance in bioaerosols of a coal mine were analyzed based on 454 pyrosequencing and real-time polymerase chain reaction（PCR）. A total of 37,191 high quality sequences were obtained and could be classified into 531, 1730 and 448 operational taxonomic units respectively for archaea, bacteria and fungi at 97% sequence similarity. The Shannon diversity index for archaea, bacteria and fungi was respectively 4.71, 6.29 and 3.86, indicating a high diversity in coal mine bioaerosols. Crenarchaeota, Proteobacteria and Ascomycota were the dominant phyla for archaea, bacteria and fungi, respectively. The concentrations of total archaea, bacteria and fungi were 1.44 × 10^8, 1.02 × 10^8 and 9.60 × 10^4cells/m^3, respectively.Methanotrophs observed in bioaerosols suggested possible methane oxidation in the coal mine. The identified potential pathogens to coal miners, such as Acinetobacter schindleri,Aeromonas cavernicola, Alternaria alternata, Aspergillus penicillioides, Cladosporium cladosporioides,and Penicillium brevicompactum were also observed. This was the first investigation of microbial diversity and abundance in coal mine bioaerosols. The investigation of microbial communities would be favorable in promoting the progress of methane control based on microbial technique and concern on coal miners＇ health.

关键词： Microbial community Bioaerosols Methanotrophs Coal mine

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Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater

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Journal of Environmental Sciences 2010年第9期22卷 1407-1412页

作者： Likun Gu Zhihui Bai Bo Jin Jianyun zhang Wenying Li Guoqiang Zhuang hongxun zhang Research Centre for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao 266101 China School of Earth and Environment Sciences the University of Adelaide Adelaide SA 5005 Australia

A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater （MGW） and potato wastewater （PW） as culture medium. The maximum yield of 6.5 × 10^9 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28℃, pH 6.0-7.5 using the mixture of MGW and PW （1：9）.

关键词： phyllosphere Paenibacillus polymyxa wastewater phospholipid fatty acids biocontrol agent

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Diversity of methanotrophs in Zoige wetland soils under both anaerobic and aerobic conditions

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Journal of Environmental Sciences 2010年第8期22卷 1232-1238页

作者： Juanli Yun Anzhou Ma Yaoming Li Guoqiang Zhuang Yanfen Wang hongxun zhang Research Center for Eco-Environment Sciences Chinese Academy of Sciences Beijing 100085 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland affects global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters detected were affiliated to type I methanotrophs. Two novel clusters （cluster 1, cluster 2） were found to relate to none of the recognized genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and aerobic soil were different from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland.

关键词： cloning library denaturing gradient gel electrophoresis methanotrophs pmoA gene real-time PCR Zoige wetland

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Molecular analysis of long-term biofilm formation on PVC and cast iron surfaces in drinking water distribution system

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Journal of Environmental Sciences 2014年第4期26卷 865-874页

作者： Ruyin Liu Junge Zhu Zhisheng Yu DevRaj Joshi hongxun zhang Wenfang Lin Min Yang College of Environmental and Resource Sciences University of Chinese Academy of Sciences Institute of Microbiology Chinese Academy of Sciences Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

To understand the impacts of different plumbing materials on long-term biofilm formation in water supply system, we analyzed microbial community compositions in the bulk water and biofilms on faucets with two different materials-polyvinyl chloride （PVC） and cast iron, which have been frequently used for more thanlO years. Pyrosequencing was employed to describe both bacterial and eukaryotic microbial compositions. Bacterial communities in the bulk water and biofilm samples were significantly different from each other. Specific bacterial populations colonized on the surface of different materials. Hyphomicrobia and corrosion associated bacteria, such as Acidithiobacillus spp., Aquabacterium spp., Limnobacter thiooxidans, and Thiocapsa spp., were the most dominant bacteria identified in the PVC and cast iron biofilms, respectively, suggesting that bacterial colonization on the material surfaces was selective. Mycobacteria and Legionella spp. were common potential pathogenic bacteria occurred in the biofilm samples, but their abundance was different in the two biofilm bacterial communities. In contrast, the biofilm samples showed more similar eukaryotic communities than the bulk water. Notably, potential pathogenic fungi, i.e., Aspergillus spp. and Candida parapsilosis, occurred in similar abundance in both biofilms. These results indicated that microbial community, especially bacterial composition was remarkably affected by the different pipe materials （PVC and cast iron）.

关键词： drinking waterbiofilm surface material pyrosequencing microbial community

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Validation of Bacteroidales-based microbial source tracking markers for pig fecal pollution and their application in two rivers of North China

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Frontiers of Environmental Science & Engineering 2020年第4期14卷 139-146页

作者： Youfen Xu Zong Li Ruyin Liu Hongxia Liang Zhisheng Yu hongxun zhang College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China

In China,pig feces is the predominant source of excrement produced b y animal husbandry.Improper use or direct discharge of pig feces can result in contamination of natural water systems.Microbial source tracking(MST)technology can identify the sources of fecal pollution in environmental water,and contribute to the management of pig fecal pollution by local environmental protection agencies.However,the accuracy of such assays can be context-dependent,and they have not been comprehensively evaluated under Chinese conditions.We aimed to compare the performance of five previously reported nig-specific MST assays(PF,Pig-Bac 1 STBR,Pig-Bac2 SYBR,Pig-1-Bac TaqMan,and Pig-2-Bac TaqMan,which are based on Bacteroidales 16S rRNA gene markers)and apply them in two rivers of North China.We collected a total of 173 fecal samples from pigs,cows,goats,chickens,humans,and horses across China.The PF assay optimized in this study showed outstanding qualitative performance and achieved 100%specificity and sensitivity.However,the two SYBR green qPCR assays(Pig-Bac1 SYBR and Pig-Bac2 SYBR)cross-reacted with most non-pig fecal samples.In contrast,both the Pig-l-Bac TaqMan and Pig-2-Bacr TaqMan assays gave 100%specificity and sensitivity.Of these,the Pig-2-Bac TaqMan assay showed higher reproducibility.Our results regarding the specificity of these pig-specific MST assays differ from those reported in Thailand,Japan,and America.Using the PF and Pig-2-Bac TaqMan assays,a field test comparing the levels of pig fecal pollution in rivers near a pig farm before and after comprehensive environmental pollution governance indicated that pig fecal pollution was effectively controlled at this location.

关键词： Microbial source tracking Pig fecal pollution 16S rRNA genem arkers Pig-specific Bacteroidales

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Effects of cotton straw amendment on soil fertility and microbial communities

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Frontiers of Environmental Science & Engineering 2012年第3期6卷 336-349页

作者： Wuren HUANG Zhihui BAI Daniel HOEFEL Qing HU Xin LV Guoqiang ZHUANG Shengjun XU Hongyan QI hongxun zhang Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China Institute for Environment and Plant Protection Chinese Academy of Tropical Agriculture SciencesDanzhou 571737China Australian Water Quality Centre South Australian Water CorporationAdelaide SA 5000Australia School of Earth and Environmental Sciences The University of AdelaideAdelaide SA 5000Australia

Maintaining soil fertility, while controlling pollution from excessive chemical fertilizer application is important for keeping soil productivity of sustainable agriculture. Variety of straws have been used and proven to be good soil amendments for increasing soil organic matter （OM） and a range of additional soil nutrients. However, little is known about the utilization of cotton straw for soil amendment. To better understand the mechanism behind cotton straw soil amendments, investigations were performed upon cucumber seedlings, where changes to soil nutrients and microbial communities were investigated. The results revealed that the cotton straw application promoted the cucumber seedling growth by significantly increasing the soil OM, available nitrogen, available phosphorus, and available potassium. The concentration of cotton straw was positively correlated to both the number of the culturable microorganisms and also the total microbial biomass within soil. Furthermore, assessment of cotton straw application using Biolog metabolic profiling and phospholipid fatty acid analysis revealed that such application increased the microbial community metabolic activity, and markedly changed the structure of microbial community. 16S rRNA gene clone library construction and phylogenetic analysis of soil bacteria revealed 7- Proteobacteria sequences dominated the cotton straw amendment soil, comprising 27.8% of the total number of analyzed sequences, while they were less represented in control soil （13.4%）. On the contrary, the Sphingobacteria （7.8%） and Verrucomicrobia （2.4%） in the cotton straw amendment soil decreased after application when compared to the control soil 15.2% and 15.2%.

关键词： cotton straw soil amendments microbialdiversity Biolog phospholipid fatty acid 16S rRNA geneclone library

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Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex

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Chinese Chemical Letters 2024年第5期35卷 330-337页

作者： Yang Qin Jiangtian Li Xuehao zhang Kaixuan Wan Heao zhang Feiyang Huang Limei Wang hongxun Wang Longjie Li Xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pancreatic Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Wuhan Polytechnic University Wuhan 430023China

Strand displacement reaction is a crucial component in the assembly of diverse DNA-based nanodevices,with the toehold-mediated strand displacement reaction representing the prevailing strategy.However,the single-stranded Watson-Crick sticky region that serves as the trigger for strand displacement can also cause leakage reactions by introducing crosstalk in complex DNA circuits.Here,we proposed the toeless and reversible DNA strand displacement reaction based on the Hoogsteen-bond triplex,which is compatible with most of the existing DNA circuits.We demonstrated that our proposed reaction can occur at pH 5 and can be reversed at pH 9.We also observed an approximately linear relationship between the degree of reaction and pH within the range of pH 5-6,providing the potential for precise regulation of the reaction.Meanwhile,by altering the sequence orientation,we have demonstrated that our proposed reaction can be initiated or regulated through the same toeless mechanism without the requirement for protonation in low pH conditions.Based on the proposed reaction principle,we further constructed a variety of DNA nanodevices,including two types of DNA logic gates that rely on pH 5/pH 9 changes for initiating and reversing:the AND gate and the OR gate.We also successfully constructed a DNA Walker based on our proposed reaction modes,which can move along a given track after the introduction of a programmable DNA sequence and complete a cycle after 4 steps.Our findings suggest that this innovative approach will have broad utility in the development of DNA circuits,molecular sensors,and other complex biological systems.

关键词： DNA strand displacement Toeless Triplex DNA strands Reversible DNA circuit DNA walker

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Substrate-induced changes in microbial community-level physiological profiles and their application to discriminate soil microbial communities

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Journal of Environmental Sciences 2008年第6期20卷 725-731页

作者： CHEN Jian XIE Huijun ZHUANG Xuliang ZHUANG Guoqiang BAI Zhihui zhang hongxun Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Bureau of Science and Technology for Resources and Environment Chinese Academy of Sciences Beijing 100864 China

The addition of simple substrates could affect the microbial respiration in soils. This substrate-induced respiration is widely used to estimate the soil microbial biomass, but little attention has been paid to its influence on the changes of community-level physiological profiles. In this study, the process of microbial communities responding to the added substrate using sole-carbon-source utilization （BIOLOG） was investigated. BIOLOG is biased toward fast-growing bacteria; this advantage was taken to detect the prompt response of the active microbial communities to the added substrate. Four soil samples from agricultural fields adjacent to heavy metal mines were amended with L-arginine, citric acid, or D-glucose. Substrate amendments could, generally, not only increase the metabolic activity of the microbial communities, but also change the metabolic diverse patterns compared with no-substrate control. By tracking the process, it was found that the variance between substrate-induced treatment and control fluctuated greatly during the incubation course, and the influences of these three substrates were different. In addition, the application of these induced changes to discriminate soil microbial communities was tested. The distance among all samples was greatly increased, which further showed the functional variance among microbial communities in soils. This can be very useful in the discrimination of microbial communities even with high similarity.

关键词： substrate-induced respiration （SIR） BIOLOG community-level physiological profiles （CLPPs）

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Effect of cypermethrin insecticide on the microbial community in cucumber phyllosphere

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Journal of Environmental Sciences 2008年第11期20卷 1356-1362页

作者： Baoguo zhang[a][c], hongxun zhang[a][c], Bo JIN[b], Ling TANG[a], Jianzhou YANG[a], Baoju LI[d], Guoqiang ZHUANG[a] Zhihui BAI[a][b] [a]Research Centre for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China [b]School of Earth Environmental Science University of Adelaide Adelaide SA 5005 Australia [c]Graduate University of Chinese Academy of Sciences Beijing 100085 China [d]Institute of Vegetables Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China

Cucumber (Cucumis sativus) is one of the most widely used vegetable in the world,and different pesticides have been extensively used for controlling the insects and disease pathogens of this plant.However,little is known about how the pesticides affect the microbial community in cucumber phyllosphere.This study was the first attempt to assess the impact of pyrethroid insecticide cyperemethrin on the microbial communities of cucumber phyllosphere using biochemical and genetic approaches.Phospholipid fatty ac...

关键词： cucumber cypermethrin insecticide microbial community phyllosphere phospholipid fatty acid （PLFA） T-RFLP

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