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Chinese Journal of Cancer Research 2017年第1期29卷 86-92页

作者： Lynne M.Ellison Yangao Man Alexander Stojadinovic hongwu xin Itzhak Avital Bon Secours Cancer Institute Bon Secours Health System Richmond VA 23226 USA Laboratory of Oncology the First People's Hospital of Jingzhou City the First Hospital and Clinical Medical School of Yangtze University Jingzhou 434008 China Laboratory of Oncology Center forMolecular Medicine Medical School Yangtze University Jingzhou 434023 China

Although gastric cancer with peritoneal carcinomatosis is associated with poor prognosis and is generally treated with palliative systemic therapy, recent studies have shown that cytoreductive surgery（CRS） and hyperthermic intraperitoneal chemotherapy（HIPEC） may prove to be an efficacious treatment option. In addition to reviewing the natural history of gastric cancer with peritoneal carcinomatosis, this mini-review examines literature on the efficacy of CRS and HIPEC as compared to chemotherapy and surgical options. Both randomized and nonrandomized studies were summarized with the emphasis focused on overall survival. In summary, CRS and HIPEC are indeed a promising treatment option for gastric cancer with peritoneal carcinomatosis and large randomized clinical trials are warranted.

关键词： Cytoreductive surgery hyperthermic intraperitoneal chemotherapy peritoneal carcinomatosis

Oncolytic Engineering of ICP34.5 and LAT of Herpes Simplex Virus Type 1

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Yangtze Medicine 2021年第2期5卷 106-116页

作者： Wenqi Cai Ying Zhang Qi Huang Ying Xiang hongwu xin Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Oncolytic virus (OV) is a kind of virus that can preferentially infect and kill tumor cells. The second oncolytic virus drug was oncolytic herpes simplex virus (oHSV) Talimogene Laherparepvec (T-VEC). HSV-1 infectious cell culture protein 34.5 (ICP34.5) and latency-associated transcript (LAT) genes are closely related to virus selective infection and latent infection. Their engineering is essential for constructing efficient and safe oHSV. We summarized the mechanisms of ICP34.5 and LAT in the course of HSV-1 infection and reviewed the engineered oHSVs. We are aimed to provide an insight in developing oHSV in the future.

关键词： Herpes Simplex Virus Oncolytic Herpes Simplex Virus Latency-Associated Transcript ICP34.5

Novel Functional Motifs of the Cell Entry Glycoprotein D for Oncolytic Herpes Simplex Viruses

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Yangtze Medicine 2021年第4期5卷 266-286页

作者： Boxu Ren Xiaoqin Liu Yingying Wang Yanning Lyu Hongyi xin Xiaochun Peng Ying Xiang Xianwang Wang hongwu xin The Second School of Clinical Medicine Health Science Center Yangtze University Jingzhou China Department of Nursing and Medical Imaging Technology Yangtze University Jingzhou China Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Institute for Infectious Diseases and Endemic Diseases Prevention and Control Beijing Center for Diseases Prevention and Control Beijing China Animal Health Biotechnology Temasek Life Sciences Laboratory National University of Singapore Singapore Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Background: Oncolytic herpes simplex virus (oHSV) have been proved effective and safe to treat tumors. Glycoprotein D (gD) has been engineered for targeting cancer cells and de-targeting normal cells successfully, however, the effectiveness and safety of oHSVs still need to be improved. Method: Here we sequenced the DNA encoding gD of our recently isolated new strain HSV-1-LXMW and compared the gD amino acid sequence with the gDs of other 7 HSV-1 and 3 HSV-2 strains. Results: Phylogenetic analysis revealed that HSV-1-LXMW is evolutionarily close to HSV-1-Patton and -KOS strains. The gD amino acid sequence alignment identified 19 conserved and 8 variable regions. We further predicted 10 new motifs in HSV gD for the first time and identified motif differences in HSV-1 and HSV-2. We summarized the gD-engineered oHSVs and found that some of the newly identified gD motifs are actually functional. Conclusion: Our results shed light on HSV gD biology and provided new directions for future gD functional studies and engineering in order to make better oHSVs.

关键词： Oncolytic Herpes Simplex Virus (oHSV) Amino Acids (AA) Glycoprotein D (gD) Cancer Treatment Reengineering Virus

Bioinformatics Analysis Revealed Potential Tumor Suppressors (KLF4/CGN), Oncogenes (SHH/LIF) and Biomarkers of Asian Stomach Adenocarcinoma

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Yangtze Medicine 2021年第2期5卷 141-156页

作者： Yang Zhou Yingying Wang Junting Cheng Ying Zhang Wenqi Cai Ziwen Han Moyu Wang Qi Huang Xiaochun Peng hongwu xin Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.

关键词： WGCNA (Weighted Correlation Network Analysis) Tumor Suppressors Oncogenes Stomach Adenocarcinoma (STAD) Hub Gene

Oncolytic Herpes Simplex Virus ICP47 Deletion Reverses Tumor Immune Evasion

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Yangtze Medicine 2018年第4期2卷 214-224页

作者： Junting Cheng Yingying Wang Ying Zhang Wenqi Cai Yang Zhou Ziwen Han Xiaoqin Liu Xianwang Wang Xiaochun Pen Ying Xiang Zhaowu Ma hongwu xin The Second School of Clinical Medicine Health Science Center Yangtze University Jingzhou China Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Laboratory Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Herpes simplex virus (HSV) is an enveloped, double-stranded DNA virus that has been used with modification as oncolytic viruses (OVs) against a number of tumor types. OVs represent a new class of therapeutic agents that promote anti-tumour responses through a dual mechanism of action that is dependent on selective tumor cell killing and the induction of systemic anti-tumour immunity. Among OVs, HSVs preferentially replicate in and lyse cancer cells, leading to in situ autovaccination, adaptive anti-virus and anti-tumor immunity. Suppression of antitumor immunity after OV therapy has been observed and the molecular and cellular mechanisms of action are recently reported. ICP47, a small protein produced by the herpes simplex virus, is considered as an important factor in the evasion of cellular immune responses in HSV-infected cells. Therefore, reviewing the research status of ICP47 is certainly helpful to improve the anti-tumor effect of oncolytic HSVs (oHSVs). Here, this review will focus on the following contents: 1) Anti-tumor mechanism of OVs;2) Functions of early HSV genes;3) The mechanism of immune escape of ICP47;4) Recombinant HSV against cancer;5) The functional verification of ICP47 deletion. This review highlights the current understanding of recombinant HSVs against cancers.

关键词： Oncolytic Virus HSV ICP47 Anti-Tumor Immunity

The Role, Mechanism and Transcriptional Regulation of LAT in Herpes Simplex Virus Latency and Reactivation

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Yangtze Medicine 2020年第1期4卷 39-53页

作者： Ying Zhang Yingying Wang Junting Cheng Wenqi Cai Ziwen Han Yang Zhou Qi Huang Moyu Wang Xiaochun Peng Xianwang Wang Zhaowu Ma Ying Xiang hongwu xin Laboratory of Oncology Center for Molecular Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Biochemistry and Molecular Biology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Pathophysiology School of Basic Medicine Health Science Center Yangtze University Jingzhou China Department of Laboratory Medicine School of Basic Medicine Health Science Center Yangtze University Jingzhou China

Herpes simplex virus (HSV) infection in the human body can be latent in neurons for long time and be reactivated leading to recurrence at high rate. Currently there is no effective clinical strategy for the prevention and treatment of the disease relapse. HSV LAT gene is expressed in large quantities and lytic genes are turned off leading to HSV latency. Disruption of the gene expression is thought to cause HSV reactivation and disease relapse. To reveal the essence of HSV latency and reactivation, we summarized and innovatively classified the role, mechanism and transcriptional regulation of LAT in HSV latency and reactivation. This review may have important implications for future studies on HSV latency and reactivation, HSV disease prevention and treatment, and safer and more effective oncolytic HSVs (oHSVs).

关键词： Herpes Simplex Virus (HSV) Oncolytic Herpes Simplex Virus (oHSV) Latency-Associated Transcript (LAT) Reactivation Immediate-Early Gene (IE Gene)

巢蛋白和α-抑制素蛋白在不同卵巢性索-间质肿瘤中的表达

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陕西医学杂志 2024年第8期53卷 1118-1123页

作者：吴宝吴崇铭 [美国]信洪武白玉勤赤峰学院中医(蒙医)学院内蒙古赤峰024005 山梨大学医学部泌尿外科日本山梨县30511-1 长江大学医学部分子医学中心肿瘤学研究室湖北荆州434023 赤峰学院基础医学院内蒙古人类遗传病研究自治区高等学校重点实验室内蒙古赤峰024005 赤峰市肿瘤医院病理重点实验室内蒙古赤峰024000

目的:探讨巢蛋白(Nestin)和α-抑制素(α-inhibin)蛋白在不同卵巢性索-间质肿瘤(SCST)中的表达情况。方法:选取卵泡膜细胞瘤、卵巢纤维瘤、成年型粒层细胞瘤、卵巢微囊性间质瘤和卵巢低分化支持-莱迪(Sertoli-Leydig)细胞瘤组织标本,制... 详细信息

目的:探讨巢蛋白(Nestin)和α-抑制素(α-inhibin)蛋白在不同卵巢性索-间质肿瘤(SCST)中的表达情况。方法:选取卵泡膜细胞瘤、卵巢纤维瘤、成年型粒层细胞瘤、卵巢微囊性间质瘤和卵巢低分化支持-莱迪(Sertoli-Leydig)细胞瘤组织标本,制备石蜡切片,行苏木精-伊红染色和α-inhibin、Nestin免疫组织化学染色。采用Spearman法分析Nestin和α-inhibin表达评分的相关性。结果:Nestin在SCST中的卵泡膜细胞瘤、卵巢纤维瘤、成年型粒层细胞瘤中呈阴性表达,在卵巢微囊性间质瘤和卵巢低分化Sertoli-Leydig细胞瘤组织中Leydig细胞呈阳性表达。α-inhibin在卵泡膜细胞瘤、卵巢纤维瘤和成年型粒层细胞瘤呈阳性表达,在卵巢微囊性间质瘤和卵巢低分化Sertoli-Leydig细胞瘤组织中呈阴性表达。Nestin与α-inhibin表达评分无相关性(均P<0.05)。结论:卵泡膜细胞瘤、卵巢纤维瘤、成人型颗粒细胞瘤组织中Nestin蛋白阴性表达,α-inhibin蛋白阳性表达。卵巢微囊性间质瘤、卵巢低分化Sertoli-Leydig细胞瘤组织中Nestin蛋白阳性表达,α-inhibin蛋白阴性表达,且Nestin与α-inhibin蛋白表达无明显关系。

关键词：卵巢性索-间质肿瘤巢蛋白 α-抑制素苏木精-伊红染色免疫组织化学染色相关性

裸鼠肺腺癌移植瘤组织中缺氧诱导因子-1α蛋白、血清蛋白和淋巴管内皮细胞透明质酸受体1蛋白表达及意义

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陕西医学杂志 2022年第12期51卷 1472-1476,1482页

作者：孔凡龙吴宝 [美国]信洪武白玉勤锦州医科大学辽宁锦州121001 赤峰学院基础医学院组织学与胚胎学教研室内蒙古赤峰024000 长江大学医学部分子医学中心肿瘤学研究室湖北荆州434023 赤峰学院医学院分子医学中心内蒙古赤峰024000 赤峰市肿瘤医院赤峰学院第二附属医院病理科内蒙古赤峰024000

目的:回顾性研究肺腺癌移植瘤裸鼠模型组织中缺氧诱导因子-1α(HIF-1α)、血清蛋白[Albumin、免疫球蛋白M(IgM)]和淋巴管内皮细胞透明质酸受体1(LYVE-1)蛋白表达及意义。方法:在裸鼠背部皮下注射肺腺癌A549细胞,建立移植瘤模型。利用活... 详细信息

目的:回顾性研究肺腺癌移植瘤裸鼠模型组织中缺氧诱导因子-1α(HIF-1α)、血清蛋白[Albumin、免疫球蛋白M(IgM)]和淋巴管内皮细胞透明质酸受体1(LYVE-1)蛋白表达及意义。方法:在裸鼠背部皮下注射肺腺癌A549细胞,建立移植瘤模型。利用活体冷冻技术(IVCT)制备标本,采用HE染色及免疫组织化学(IHC)染色分析HIF-1α、Albumin、IgM和LYVE-1蛋白的表达。结果:在A549移植瘤细胞中HIF-1α蛋白表达呈阴性。肺腺癌侵袭组织中Albumin和IgM蛋白呈阳性的功能血管密度高于非侵袭组织[(14.80±1.03)个/高倍视野(HPF)与(3.50±0.53)个/HPF,P<0.01]。肺腺癌侵袭组织中LYVE-1蛋白呈阳性的淋巴管密度高于非侵袭组织[(25.90±4.43)个/HPF与(3.60±0.52)个/HPF,P<0.01]。结论:HIF-1α蛋白表达与肺腺癌侵袭和功能性血管、淋巴管密度无关,但是功能性血管和淋巴管密度与肺腺癌的侵袭、发展密切相关。

关键词：肺腺癌移植瘤侵袭组织缺氧诱导因子-1α 淋巴管内皮细胞透明质酸受体1 活体冷冻技术功能性血管裸鼠

An Evaluation Method for Automotive Technical and Comprehensive Performance

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Automotive Innovation 2023年第2期6卷 231-243页

作者： Mengfei Liu xinyu Ouyang Ruikai Lu Zijun Hao Raphael Blumenfeld xin Tang Gang Lei hongwu Ouyang School of Mechanical and Electrical Engineering Central South UniversityChangsha 410083China State Key Laboratory of High‑Performance Complex Manufacturing Central South UniversityChangsha 410083China University of Sheffield Sheffield S102TNUK Cavendish Laboratory University of CambridgeCambridge CB21TNUK

An objective evaluation scheme for automotive technical and comprehensive performance could provide critical and instructive insights for academic research,engineering practice,and commercial marketing of *** this paper,the technical performance index A=S∕T_(1)⋅T_(2)(m∕(s^(2)⋅L))and comprehensive performance index F=M⋅S∕T_(1)⋅T_(2),(kN⋅L^(−1),where M is the vehicle mass)are formulated by incorporating the vehicle 0–100 km⋅h^(−1) acceleration duration T_(1),100–0 km⋅h^(−1) braking duration T_(2),and fuel economy S(mileage per liter fuel at constant speed)to assess the vehicle’s longitudinal dynamic performance.A and F offer a clear physical implication of a vehicle’s acceleration capability and traction efficiency acquired per unit of fuel consumption,*** indexes are used for wide case studies of popular market sedans and SUVs of joint ventures(JVs)and domestic brands in China over the last 17 *** findings prove that this approach could be effectively and reliably utilized for the objective evaluation and analysis of the technical and comprehensive performance of automotive models.

关键词： Automobile Technical performance evaluation Comprehensive performance evaluation Sedan SUV

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强肝胶囊治疗慢性乙型肝炎的临床效果

中国医药导报 2021年第10期18卷 80-83页

作者：王华杨柳明黄玲信洪武赵延龙许俊考黄春荣冯广贵广东省廉江市人民医院中医科广东廉江524400 长江大学医学院湖北荆州434023

目的探讨强肝胶囊对慢性乙型肝炎(CHB)肝纤维化的疗效。方法选择2017年7月-2019年7月广东省廉江市人民医院收治的100例CHB患者为研究对象,依据随机数字表法将其分为对照组和治疗组,每组各50例。对照组给予葡醛内酯片、复合维生素B片,治... 详细信息

目的探讨强肝胶囊对慢性乙型肝炎(CHB)肝纤维化的疗效。方法选择2017年7月-2019年7月广东省廉江市人民医院收治的100例CHB患者为研究对象,依据随机数字表法将其分为对照组和治疗组,每组各50例。对照组给予葡醛内酯片、复合维生素B片,治疗组给予强肝胶囊,共治疗6个月。比较两组丙氨酸氨基转移酶(ALT)、总胆红素(TBil)、白蛋白(ALB)、凝血酶原时间(PT)、血小板衍生生长因子BB(PDGF-BB)、转化生长因子β1(TGF-β1)、基质金属蛋白酶1(MMP-1)、基质金属蛋白酶组织抑制因子1(TIMP-1),观察两组中医体质变化,记录不良反应的发生情况。结果治疗后,两组ALT、TBil水平低于治疗前,PT短于治疗前,ALB水平高于治疗前(均P0.05)。治疗后,对照组PDGF-BB、TGF-β1、MMP-1、TIMP-1水平与治疗前比较,差异无统计学意义(P>0.05);治疗组PDGF-BB、TGF-β1、TIMP-1水平低于治疗前,MMP-1水平高于治疗前(均P0.05);治疗组体质变化与治疗前比较,差异有统计学意义(P0.05)。结论强肝胶囊能有效改善CHB患者肝纤维化指标及中医体质。

关键词：强肝胶囊慢性乙型肝炎肝纤维化中医体质