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细胞焦亡在抗肿瘤纳米递送系统中的研究进展

医药导报 2024年第8期43卷 1230-1235页

作者：陈燕石三军糜丹丹王茹静肖洪涛四川省肿瘤临床医学研究中心、四川省肿瘤医院研究所、四川省肿瘤防治中心、电子科技大学附属肿瘤医院药学部成都610041 成都中医药大学药学院成都611137

细胞焦亡是一种由焦孔素家族介导的炎性细胞死亡方式,近年来在肿瘤治疗方面取得了较大的进展。细胞焦亡可以激活机体抗肿瘤免疫应答,而肿瘤免疫治疗是肿瘤治疗的一个新领域,因此调控细胞焦亡对于肿瘤治疗具有巨大潜力。纳米技术具有精... 详细信息

细胞焦亡是一种由焦孔素家族介导的炎性细胞死亡方式,近年来在肿瘤治疗方面取得了较大的进展。细胞焦亡可以激活机体抗肿瘤免疫应答,而肿瘤免疫治疗是肿瘤治疗的一个新领域,因此调控细胞焦亡对于肿瘤治疗具有巨大潜力。纳米技术具有精准靶向及持续释放的优点,亦是肿瘤精准治疗的核心手段。基于此,该文总结了激活细胞焦亡的药物以及应用纳米技术辅助促进细胞焦亡来参与肿瘤治疗的纳米制剂,并对其在细胞焦亡上作用机制及应用进行综述,以期为基于细胞焦亡的抗肿瘤治疗提供参考。

关键词：细胞焦亡焦孔素家族抗肿瘤纳米递送系统

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微针递送系统在皮肤肿瘤治疗中的研究进展

医药导报 2024年第8期43卷 1235-1244页

作者：罗伊婷余婷邱悦廖龙飞马雪肖洪涛漆婷婷四川省肿瘤临床医学研究中心四川省肿瘤医院·研究所四川省癌症防治中心电子科技大学附属肿瘤医院药学部成都610041

微针作为一种新兴的给药技术,可以穿透皮肤角质层,产生微米尺寸的机械通道,无痛、微创和高效地将药物递送至表皮层或真皮层,从而发挥局部或全身治疗作用。该文综述了目前微针用于各类疾病治疗的临床试验进展,并对各类型微针的特点进行阐... 详细信息

微针作为一种新兴的给药技术,可以穿透皮肤角质层,产生微米尺寸的机械通道,无痛、微创和高效地将药物递送至表皮层或真皮层,从而发挥局部或全身治疗作用。该文综述了目前微针用于各类疾病治疗的临床试验进展,并对各类型微针的特点进行阐述,总结了微针在皮肤肿瘤领域的化学药物治疗、光热和光动力治疗、免疫治疗、基因治疗以及联合治疗的最新研究进展,为进一步研究微针用于皮肤肿瘤的治疗提供思路和方向。

关键词：微针递送系统皮肤肿瘤药物递送

食物对口服靶向抗肿瘤药物代谢影响的研究进展

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肿瘤预防与治疗 2024年第3期37卷 243-251页

作者：廖强徐晓菲肖腊梅肖洪涛程凯周行阴骏王欢四川大学华西医院临床药学部药剂科成都610041 四川省德阳市第二人民医院药学部四川德阳618000 四川省肿瘤临床医学研究中心四川省肿瘤医院·研究所四川省癌症防治中心电子科技大学附属肿瘤医院药学部成都610041 四川省肿瘤临床医学研究中心四川省肿瘤医院·研究所四川省癌症防治中心电子科技大学附属肿瘤医院肿瘤内科成都610041 四川省肿瘤临床医学研究中心四川省肿瘤医院·研究所四川省癌症防治中心电子科技大学附属肿瘤医院放疗中心成都610041 成都医学院药学院成都610500

部分食物对口服靶向抗肿瘤药物的代谢具有临床意义的影响,包括:西柚可影响尼罗替尼、舒尼替尼、伊马替尼、克唑替尼、达沙替尼、厄洛替尼、拉帕替尼、帕唑帕尼、依维莫司、奥拉帕利、塞瑞替尼、凡德他尼、阿昔替尼的代谢;酸橙可影响奥... 详细信息

部分食物对口服靶向抗肿瘤药物的代谢具有临床意义的影响,包括:西柚可影响尼罗替尼、舒尼替尼、伊马替尼、克唑替尼、达沙替尼、厄洛替尼、拉帕替尼、帕唑帕尼、依维莫司、奥拉帕利、塞瑞替尼、凡德他尼、阿昔替尼的代谢;酸橙可影响奥拉帕利的代谢;蔓越莓对伊马替尼、生姜对克唑替尼、苹果对厄洛替尼、人参对伊马替尼及达沙替尼、芹菜对达沙替尼、烟草对厄洛替尼的代谢存在影响;合并使用质子泵抑制剂时,可乐可影响厄洛替尼的代谢。临床用药需要重视食物对口服靶向抗肿瘤药物代谢的影响,注意合理搭配药物与食物,保障药物的有效性和安全性。

关键词：食物口服靶向抗肿瘤药物代谢影响

Diagnosis of Helicobacterpyloriinfection and diseases associated with Helicobacter pylori by Helicobacter pylori outer membrane proteins

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World Journal of Gastroenterology 2004年第23期10卷 3464-3469页

作者： ZhengJiang Ai-LongHuang xiao-hongtao Pi-LongWang DepartmentofGastroenterology theFirstAffiliatedHospitalChongqingUniversityofMedicalSciencesChongqing400016China InstituteofViralHepatitis ChongqingUniversityofMedicalSciencesChongqing400010China

AIM:TO examine the serological response of patients with upper gastrointestinal diseases and Helicobocter pylori(H pylon) infection to two Hpyloriouter membrane proteins(OMPs) (M,18 000 and M,26 000)acquired by gene recombinant technique,and to determine the diagnostic significance of serological tests derived from these OMPs. METHODS:Recombinant vectors encoding the two Hpylori OMPs were used to transform and express in BL21(DE3) *** purification with Ni^(2+)-NTA agarose resin,colloid gold kits were prepared with purified recombinant proteins to detect H pylori infectJon and H pylori-associated diseases by the immunity-marker *** selected 150 patients with Hpyloriinfection and digestive symptoms without previous treatment,induding chronic gastritis(n=60),duodenal ulcer (n=30),gastric ulcer(n=30),and gastric cancer(n=30). As controls,33 Hpylori-negative healthy volunteers were also *** samples were collected from all subjects,and the antibodies to specific proteins of H pylori were tested with the colloid gold test *** sensitivity, specificity and accuracy of the colloid gold tests were evaluated,by using the combination of standard diagnostic methods(^(13)C urea breath test and bacteria culture)and classic enzyme-linked immunosorbent assay(ELISA)as reference. RESULTS:After purification with Ni^(2+)-NTA agarose resin, the purity of recombinant fusion proteins was about 95%. The recombinant fusion proteins were recognized by the specific monodonal antibodies against the two Hpylori OMPs, as demonstrated by the *** the 150 serum samples from patients infected with Hpylori 141(94.0%)responded positively to the recombinant protein with M_126 000,while the seropositive rates were 95.0%,96.7%,96.7% and 90.0% for patients with H pylori-associated chronic gastritis, duodenal ulcer,gastric ulcer,and gastric cancer respectively. The sensitivity,specificity,and accuracy of the colloid gold kit with M_1 26 000 protein were 94.0%,97.0%,an

关键词：诊断哈比特属幽门菌伴随物膜蛋白重组器官

Construction and characterization of bivalent vaccine candidate expressing HspA and M_r18 000 OMP from Helicobacterpylori

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World Journal of Gastroenterology 2003年第8期9卷 1756-1761页

作者： ZhengJiang Ai-LongHuang xiao-hongtao Pi-LongWang DepartmentofGastroenterology theFirstAffiliatedHospitalChongqin-gUniversityofMedicalSciencesChongqing400016China InstituteofViralHepatitis ChongqingUniversityofMedicalSciencesChongqing400010China

AIM: To construct a recombinant vector which can express outer membrane protein (OMP) with Mr18 000 and heat shock protein A (HspA) from Helicobacter pylori(***)in *** BL21, and to exploit the possibility for obtaining the vaccine conferring protection from H. pylori ***: The target gene of HspA was amplified from *** chromosome by PCR, and then inserted into the prokaryotic expression vector pET32a (+) by restrictive endonuclease enzyme kpn Ⅰ, BamH Ⅰ simultaneously. The recombinant vector was used to sequence, and then together with pET32a (+)/Omp18, digested by restrictive endonuclease enzyme Hind Ⅲ and BamH Ⅰ simultaneously. pET32a(+)/HspA and Omp18 were recovered from 1% agarose gel by gel kit, and ligated with T4 ligase by BarmH Ⅰ digested viscidity end. The recombinant plasmid of pET32a(+)/HspA/Omp18 was transformed and expressed in E. coli BL21 (DE3) under induction of IPTG. After purification, its antigenicity of the fusion protein was detected by Western ***: Enzyme digestion analysis and sequencing showed that the target genes were inserted into the recombinant vector, composed of 891 base pairs, encoded objective polypeptides of 297 amino acid residues. Compared with GenBank reported by Tomb et al, there were 1.3 %and 1.4 % differences in obtained H. pylori nucleotide sequence and amino acid residues, respectively. SDS-PAGE analysis showed that relative molecule mass (Mr) of the expressed product was Mr 51 000,Mr of protein expressed by pET32a (+) was about Mr 20 000, and soluble expression product accounted for 18.96 % of total bacterial *** purification with Ni+2-NTA agarose resins, the purification of recombinant fusion protein was about 95 %. Western blot showed that recombinant fusion protein could be recognized by the patients′ serum infected with H. pylori and anti-Omp18 monoclone, suggesting that this protein had good ***: The gene coding for H. pylori Mr18 000OMP and HspA was clone

关键词：幽门螺杆菌疫苗外膜蛋白 Mr18000 热休克蛋白A

Diet and feeding behavior of a group of high-altitude rhesus macaques: high adaptation to food shortages and seasonal fluctuations

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Current Zoology 2023年第3期69卷 304-314页

作者： Kechu Zhang Fazal Karim Zuxiang Jin hongtao xiao Yongfang Yao Qingyong Ni Bajin Li Wangjia Pu-Cuo Zhonghao Huang Huailiang Xu College of Life Science Sichuan Agricultural UniversityNo.46 Xinkang RoadYucheng DistrictYa'anSichuan 625014China Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Guangxi Normal University) Ministry of EducationNo.1Yanzhong RoadYanshan DistrictGuilingGuangxi 541006China College of Animal Science and Technology Sichuan Agricultural UniversityNo.211 Huimin RoadWenjiang DistrictChengduSichuan 611130China Gexigou National Nature Reserve Administration Yajiang Forestry and Grassland AdministrationNo.88 Jiefang RoadYajiang CountyGanziSichuan 627450China

Diet and feeding behavior data are crucial to a deep understanding of the behavioral response and adaptation of primates to a high-altitude *** August 2019 to June 2021,we collected data on the feeding behavior of a high-altitude rhesus macaque Macaca mulatta group from Yajiang County,Western Sichuan Plateau,which has an altitude of over 3,500 *** results showed that feeding(33.0±1.8%)and moving(28.3±2.6%)were the dominant behavior of rhesus *** ate 193 food items,comprising 11 food categories from 90 *** study found that plant roots(30.9±30.1%)and young leaves(28.0±33.1%)were the main foods eaten by *** preferred foods of rhesus macaques were young leaves,fruits,and seeds,and the consumption of these items was positively correlated with its food *** the availability of preferred foods was low,macaques took plant roots,barks,and fallen leaves as fallback *** particular,roots were a dominant food item in winter,and this way of feeding became a key survival *** results suggest that,facing the relative scarcity and strong seasonal fluctuations of food resources in high-altitude habitat,macaques adopt active foraging strategies,relying on a variety of food species and adjusting flexibly their food choices based on food availability,which may help to maximize the energy efficiency of high-altitude macaques.

关键词： diet foraging strategy fallback food high-altitude habitat Macaca mulatta

On the topological surface states of the intrinsic magnetic topological insulator Mn-Bi-Te family

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National Science Review 2024年第2期11卷 50-64页

作者： Yuan Wang xiao-Ming Ma Zhanyang Hao Yongqing Cai hongtao Rong Fayuan Zhang Weizhao Chen Chengcheng Zhang Junhao Lin Yue Zhao Chang Liu Qihang Liu Chaoyu Chen Shenzhen Institute for Quantum Science and Engineering(SIQSE)and Department of Physics Southern University of Science and Technology(SUSTech)

We review recent progress in the electronic structure study of intrinsic magnetic topological insulators（MnBi2Te4）·（Bi2Te3）n（n = 0, 1, 2, 3） family. Specifically, we focus on the ubiquitously（nearly）gapless behavior of the topological Dirac surface state observed by photoemission spectroscopy, even though a large Dirac gap is expected because of surface ferromagnetic order. The dichotomy between experiment and theory concerning this gap behavior is perhaps the most critical and puzzling question in this frontier. We discuss various proposals accounting for the lack of magnetic effect on the topological Dirac surface state, which are mainly categorized into two pictures, magnetic reconfiguration and topological surface state redistribution. Band engineering towards opening a magnetic gap of topological surface states provides great opportunities to realize quantized topological transport and axion electrodynamics at higher temperatures.

关键词： intrinsic magnetic topological insulator topological surface states magnetic gap magnetic reconfiguration topological surface state redistribution van der Waals spacing expansion

Ruxolitinib improves the inflammatory microenvironment,restores glutamate homeostasis,and promotes functional recovery after spinal cord injury

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Neural Regeneration Research 2024年第11期19卷 2499-2512页

作者： Jiang Cao xiao Yu Jingcheng Liu Jiaju Fu Binyu Wang Chaoqin Wu Sheng Zhang hongtao Chen Zi Wang Yinyang Xu Tao Sui Jie Chang xiaojian Cao Department of Orthopedics The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Trauma Surgery Subei People’s Hospital of JiangsuClinical Medical College of Yangzhou UniversityYangzhouJiangsu ProvinceChina Department of Orthopedics Zhongda HospitalSoutheast UniversityNanjingJiangsu ProvinceChina Department of Orthopedic Surgery Nanjing Drum Tower HospitalThe Affiliated Hospital of Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina

The inflammatory microenvironment and neurotoxicity can hinder neuronal regeneration and functional recovery after spinal cord ***,a JAK-STAT inhibitor,exhibits effectiveness in autoimmune diseases,arthritis,and managing inflammatory cytokine *** studies have shown the neuroprotective potential of ruxolitinib in neurological trauma,the exact mechanism by which it enhances functional recovery after spinal cord injury,particularly its effect on astrocytes,remains *** address this gap,we established a mouse model of T10 spinal cord contusion and found that ruxolitinib effectively improved hindlimb motor function and reduced the area of spinal cord *** sequencing analysis showed that ruxolitinib alleviated inflammation and immune response after spinal cord injury,restored EAAT2 expression,reduced glutamate levels,and alleviated excitatory ***,ruxolitinib inhibited the phosphorylation of JAK2 and STAT3 in the injured spinal cord and decreased the phosphorylation level of nuclear factor kappa-B and the expression of inflammatory factors interleukin-1β,interleukin-6,and tumor necrosis factor-α.Additionally,in glutamate-induced excitotoxicity astrocytes,ruxolitinib restored EAAT2 expression and increased glutamate uptake by inhibiting the activation of STAT3,thereby reducing glutamate-induced neurotoxicity,calcium influx,oxidative stress,and cell apoptosis,and increasing the complexity of dendritic ***,these results indicate that ruxolitinib restores glutamate homeostasis by rescuing the expression of EAAT2 in astrocytes,reduces neurotoxicity,and effectively alleviates inflammatory and immune responses after spinal cord injury,thereby promoting functional recovery after spinal cord injury.

关键词： astrocytes EAAT2 excitotoxicity glutamate homeostasis JAK-STAT pathway locomotor function neurotoxicity ruxolitinib spinal cord injury transcriptome analysis

Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori

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World Journal of Gastroenterology 2004年第21期10卷 3205-3207页

作者： Dao-RongChen Ai-LongHuang xiao-hongtao Pi-LongWang ZhengJiang DepartmentofGastroenterology theFirstAffiliatedHospitalChongqingUniversityofMedicalSciencesChongqing400016China InstituteofViralHepatitis ChongqingUniversityofMedicalSciencesChongqing400010China

AIM: To construct a recombinant E. col/strain that would highly express the proinflammatory outer membrane protein of human Helicobacter pylori ( H pylon).METHODS: The oipA DNA was amplified by PCR, inserted into pET-32a, and transformed into Top10 E. col/strain. This recombinant plasmid of Top10 was sent out for nucleotide sequence analysis. Finally this sequence AF479754 was compared with HP0638 and ***: The sequence of the aim gene was obtained. It had 924 base pairs. The identity was 95.32% against HP0638, 95.02% against JHP0581, which was higher than the identity between HP0638 and ***: Although the aim gene was obtained, but it was different from the published sequence of GenBank. It is not clear what makes this difference. Maybe it is because different strain was used or because there were some variations. So more researches are required to prove it.

关键词：无性繁殖基因编码 oipA 外隔膜蛋白哈比特属幽门菌基因序列