检索结果-南通市图书馆

Phylogenetic Analysis of Epibacterial Communities on the Surfaces of Four Red Macroalgae

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2014年第6期13卷 1025-1032页

作者： WU hongqing LIU Min zHANG Wuchang XIAO Tian Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of Sciences (CAS) School of Marine Science and Engineering Hebei University of Technology Graduate University of Chinese Academy of Sciences Key Laboratory of Biology and Genetic Resources of Tropical Crops Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural Science (CATAS)

Macroalgal surfaces are prone to being attached by bacteria. Epibacterial community structures on marine macroalgae are host-specific but temporally and spatially variable. In this study, we investigated the structure of epibacterial communities on the surfaces of four red macroalgae, Gracilaria lemaneiformis, Gloiopeltisfurcata, Mazzaella sp. and Porphyra yezoensis, by analyzing the sequences of 16S rRNA gene libraries. Healthy individuals of all macroalgae species were collected in winter from a farm at Dalian, China. The results showed that the epibacterial communities were mainly dominated by ct-Proteobacteria, 7-Proteobacteria and Bacteroidetes. Deinococcus-Thermus, Spirochaetes and e-Proteobacteria were also found. The majority of cloned sequences shared the greatest similarity to those of culturable organisms. A large portion of sequences from the ct-Proteobacteria homed in Roseobacter clade, i.e., genera Ahrensia, Roseovarius, Litoreibacter, Octadecabacter, Thaiassobacter and Sulfitobacter, while members of Bacteroidetes mainly belonged to family Flavobacteriaceae. The cloned sequences could be separated into 66 OTUs at 0.01 distance value, and rare common OTUs were found among libraries. At genus level, Pseudoa#eromonas dominated Gr. lemaneiformis and GI. furcata libraries, accounting for 72.2% and 47.3%, respectively. Sulfitobacter dominated P. yezoensis library, accounting for 35.4%. A previously undefined cluster within Deinococcus-Thermus dominated Mazzaella sp. library, accounting for 24.6% of the all. These results indicated that a broad range of bacteria inhabited the surfaces of these macroalgae.

关键词： epibacterial community red alga 16S rRNA gene

工程教育专业认证校级教学管理制度文件规范化设计

在线全文

学校读者我要写书评

暂无评论

中国冶金教育 2024年第5期 8-13页

作者：杨旭李擎罗熊宋洪庆崔家瑞北京科技大学自动化学院北京100083 北京科技大学教务处北京100083

通过三年的探索,形成了工程教育校级教学管理制度文件规范化设计模式。教务处通过校级教改重点项目组建制度文件编制团队,采用文件梳理、校外调研、专家咨询、校内讨论等手段,设计了校级规范化教学管理制度文件的框架。围绕工程教育认... 详细信息

通过三年的探索,形成了工程教育校级教学管理制度文件规范化设计模式。教务处通过校级教改重点项目组建制度文件编制团队,采用文件梳理、校外调研、专家咨询、校内讨论等手段,设计了校级规范化教学管理制度文件的框架。围绕工程教育认证七大指标体系,编制/修订了29项校级制度文件(另含20个工作模板)。通过专业实施和意见反馈,不断对文件进行改进,形成更具普适性和操作性强的校级层面教学管理制度。制度文件出台后,对已认证专业的持续改进起到了重要的支撑作用,同时对拟认证专业也实现了有效指导,保证了工程教育的规范化运行和常态化建设。

关键词：工程教育专业认证校级层面制度建设持续改进规范化设计

Direct cloning and transplanting of large DNA fragments from Escherichia coli chromosome

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2016年第10期59卷 1034-1041页

作者： Ying zhu Yan Yang Pingping Den Yong Huang Mengxiang Ni hongqing Fang Institute of Biotechnology Academy of Military Medical Sciences Institute of Life Science and technology China Pharmaceutical University Institute of Health Sciences Anhui University Institute of Microbiology and Epidemiology Academy of Military Medical Sciences

We applied a resistance split-fusion strategy to increase the in vivo direct cloning efficiency mediated by Red recombination. The cat cassette was divided into two parts： cma （which has a homologous sequence with cmb） and cmb, each of which has no resistance separately unless the two parts are fused together. The crab sequence was integrated into one flank of a target clon- ing region in the chromosome, and a linear vector containing the cma sequence was electroporated into the cells to directly capture the target region. Based on this strategy, we successfully cloned an approximately 48 kb DNA fragment from the E. coli DH1-z chromosome with a positive frequency of approximately 80%. Combined with double-strand breakage-stimulated homologous recombination, we applied this strategy to successfully replace the corresponding region of the E. coli DH36 chromosome and knock out four non-essential genomic regions in one step. This strategy could provide a powerful tool for the heterologous expression of microbial natural product biosynthetic pathways for genome assembly and for the functional study of DNA sequences dozens of kilobases in length.

关键词： Red homologous recombination resistance split-fusion target cloning transferring

低碳醇汽油发动机燃烧分析实验平台设计及教学应用

在线全文

学校读者我要写书评

暂无评论

实验技术与管理 2024年第9期41卷 118-123页

作者：冯洪庆牛振泽张秀霞马睿修王新伟林日亿姜烨中国石油大学(华东)新能源学院山东青岛266580

为探究低碳醇掺混汽油的醇基燃料在缸内具体的燃烧状态和燃烧过程,该文基于自然吸气式气道喷射汽油发动机搭建实验平台,并改进了燃烧分析功能。基于实验平台开展了3种燃料(92号汽油、甲醇比例85%的甲醇汽油、50%异丙醇掺混的92号汽油)... 详细信息

为探究低碳醇掺混汽油的醇基燃料在缸内具体的燃烧状态和燃烧过程,该文基于自然吸气式气道喷射汽油发动机搭建实验平台,并改进了燃烧分析功能。基于实验平台开展了3种燃料(92号汽油、甲醇比例85%的甲醇汽油、50%异丙醇掺混的92号汽油)的缸内燃烧实验,分析了醇基燃料燃烧状和在发动机中的燃烧性能。实验平台可完成动力性、经济性分析等测试项目,并可对缸压、放热率以及燃料自燃性质进行测量分析。

关键词：燃烧分析低碳醇燃料燃烧状态发动机性能

Flavanols from the Camellia sinensis *** and their hypoglycemic and hypolipidemic activities

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2017年第3期7卷 342-346页

作者： Xin Wang Quan Liu Hongbo zhu hongqing Wang Jie Kang zhufang Shen Ruoyun Chen State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

α-Glucosidase and lipase inhibitors play important roles in the treatment of hyperglycaemia and dyslipidemia. To identify novel naturally occurring inhibitors, a bioactivity-guided phytochemical research was performed on the pu-erh tea. One new flavanol, named(–)-epicatechin-3-O-(z)-coumarate(1), and 16 known analogs(2–17) were isolated from the aqueous extract of the pu-erh tea. Their structures were determined by spectroscopic and chemical methods. Furthermore, the water extract of pu-erh tea and its fractions exhibited inhibitory activities against α-glucosidases and lipases in vitro;compound 15 showed moderate inhibitory effect against sucrase with an IC_(50) value of 32.5 μmol/L and significant inhibitory effect against maltase with an IC_(50) value of 1.3 μmol/L. Compounds 8, 10, 11 and15 displayed moderate activity against a lipase with IC_(50) values of 16.0, 13.6, 19.8, and 13.3 μmol/L,respectively.

关键词： Camellia sinensis var. assamica Pu-erh tea Flavanol Hypoglycemic Hypolipidemic

基于CT深度学习鉴别胸腺瘤组织学分型的临床应用价值

在线全文

学校读者我要写书评

暂无评论

齐齐哈尔医学院学报 2024年第5期45卷 458-464页

作者：徐静雅翟建魏逸喻泓清范莉芳皖南医学院第一附属医院放射科安徽芜湖241000 皖南医学院医学影像学院

目的研究基于CT深度学习鉴别胸腺瘤组织学分型的临床应用价值。方法回顾性分析2014年1月—2023年3月于本院经术后病理证实的179例胸腺瘤患者的临床资料,按7︰3比列将研究对象分为训练集(n=125)和验证集(n=54)。本研究采用ResNet50作为... 详细信息

目的研究基于CT深度学习鉴别胸腺瘤组织学分型的临床应用价值。方法回顾性分析2014年1月—2023年3月于本院经术后病理证实的179例胸腺瘤患者的临床资料,按7︰3比列将研究对象分为训练集(n=125)和验证集(n=54)。本研究采用ResNet50作为卷积神经网络模型,提取深度学习特征,并运用主成分分析(PCA)、相关性分析和最小绝对收缩和选择算子(LASSO)算法筛选出最优深度学习特征,建立鉴别胸腺瘤组织学分型的深度学习模型(DTL Signature)。利用Logistic回归构建临床模型、深度学习模型和联合模型。绘制受试者工作特征曲线(ROC),并计算曲线下面积(AUC),评估3种模型鉴别胸腺瘤组织学分型的临床效能。结果筛选出11个CT平扫深度学习特征,并建立深度学习模型,在训练集和验证集中,其受试者工作曲线(ROC)下面积(AUC)分别为0.877、0.795,均高于临床模型的AUC,但差异均无统计学意义(z=1.903、1.033,P均>0.05)。在训练集和验证集中,临床模型与深度学习模型构建联合模型,其ROC的AUC分别为0.890、0.841,均高于临床模型,且差异有统计学意义(z=2.647、3.041,P均<0.05)。结论基于CT平扫的深度学习联合模型有利于鉴别胸腺瘤组织学分型,临床获益较高,值得临床推广应用。

关键词：胸腺瘤组织学分型深度学习电子计算机断层扫描

Preparation and Properties of Crystallizable Glass/Al_2O_3 Composites for LTCC Material

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2011年第6期26卷 1174-1178页

作者：邵辉周洪庆 College of Materials Science and Engineering Nanjing University of Technology

The investigated low temperature Co fired ceramics（LTCC） composite of 60wt% CaO-Al2O3-B2O3-SiO2 glass and 40wt% α-Al2O3 as a filler is a non-reactive system, which is a critical part of the low temperature Co fired ceramics process. Through a study on densification process, the phase transformation and microstructure can be revealed. Its composites typically consist of CaO-Al2O3-B2O3-SiO2 glass and α-Al2O3 powders of average particle size （D50=3.49 mm）. The sintering behavior, phase evaluation, sintered morphology, and microwave dielectric properties were investigated. In the fire range of 800 to 900 ℃, the composites were crystallized after completion of densification. It is found that the composites start to densify at 825 ℃, simultaneously, the dielectric constant （εr） reaches its maximum. With increasing heat-treatment temperatures, due to the loose microstructure of the material, tanδ increases slightly. The last of the sintered samples were identified as partly Anorthite at 850 ℃. At that temperature it has εr of 7.9 and tanδ less than 1×10-3, and can be used as a promising LTCC material.

关键词： glass-ceramics densification precipitation LTCC materials

在线全文

学校读者我要写书评

暂无评论

普兰廷加的恰当功能主义

自然辩证法通讯 2017年第4期39卷 37-45页

作者：方红庆浙江工业大学政管学院浙江杭州310023 厦门大学知识论与认知科学中心福建厦门361005

普兰廷加主张知识是被保证的真信念,而保证由三个密切相关的核心要件构成:恰当功能、设计计划和可靠性。无疑,恰当功能主义存在诸多问题,如与可靠论的区别问题、知识论的本体论蕴含问题等,这些问题的根源在于普兰廷加把为知识寻找"第三... 详细信息

普兰廷加主张知识是被保证的真信念,而保证由三个密切相关的核心要件构成:恰当功能、设计计划和可靠性。无疑,恰当功能主义存在诸多问题,如与可靠论的区别问题、知识论的本体论蕴含问题等,这些问题的根源在于普兰廷加把为知识寻找"第三个条件"的后葛梯尔式知识论路径视为理所当然,导致其既不能坚持认知官能本身的探究,又不能在新的视角下重新审视知识的构成与本质。因此,为了弥补这两方面的缺陷,一种合理的建议就是引入当代德性知识论所实践的亚里士多德主义,即深入挖掘人的理智德性及其认知意义。

关键词：保证恰当功能设计计划可靠性理智德性

Radiotherapy delivery error detection with electronic portal imaging device (EPID)-based in vivo dosimetry

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2023年第8期136卷 998-1000页

作者： Xueying Yang Lisheng Geng David Huang Kaiwen Li hongqing z.uang Jing Cai Ruijie Yang School of Physics Beihang UniversityBeijing 102206China Department of Radiation Oncology Peking University Third HospitalBeijing 100191China Beijing Advanced Innovation Center for Big Data-Based Precision Medicine School of Medicine and EngineeringBeihang UniversityBeijing 100191China Medical Physics Graduate Program Duke Kunshan UniversityKunshanJiangsu 215316China Medical Management Department CAS Ion Medical Technology Co.Ltd.Beijing 100190China Department of Health Technology and Informatics The Hong Kong Polytechnic UniversityHong Kong 999077China

To the Editor:Due to the highly complex nature of intensity-modulated radiotherapy(IMRT)planning and delivery,patient specific quality assurance(PSQA)should be implemented to assure the reliability of treatment delive... 详细信息

关键词： IMRT treatment vivo