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Science China Chemistry 2014年第6期57卷 918-922页

作者： liU li li hongmei DAI JingCao Institute of Materials Physical Chemistry Huaqiao University

Ce/Sm ions are covalently grafted to a Keggin polyoxomolybdate cluster[PMo12O40]3-to yield interesting fluorescent-photodarkening molecular *** photoresponce processes are observed to be activated via energy transfer ... 详细信息

关键词： lanthanide-decorated polyoxomolybdates photocoloration solar-furnace photoactivation energy transfer

Nutrient Structure of the Taiwan Warm Current and Estimation of Vertical Nutrient Fluxes in Upwelling Areas in the East China Sea in Summer

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Journal of Ocean University of China 2014年第4期13卷 613-620页

作者： SHI Xiaoyong li hongmei WANG Hao College of Chemistry and Chemical Engineering Ministry of Education Ocean University of China Key Laboratory of Marine Chemistry Theory and Technology Ministry of Education Ocean University of China National Marine Hazard Mitigation Service Fujian Marine Environment and Fishery Resource Monitoring Center

Based on field data for nutrients collected on the continental shelf of the East China Sea(ECS) during summer 2006, the structure and variations of nutrients in every water mass related to the Taiwan Warm Current(TWC) were analyzed. The supplementary effect of nutrient of upwelling on harmful algal blooms(HABs) in the ECS was also estimated, based on upwelling data. Then the maintenance contribution of nutrient of upwelling to HABs was assessed. The results showed that N/P ratio is fairly low in both surface and deep layers of the TWC, which possibly controls nutrient structure of the HABs-frequently-occuring areas. In upwelling areas, the rate of phosphate(PO4-P) uptake exceeds that of nitrate(NO3-N) of the TWC. The TWC may relieve PO4-P limitation during the process of HABs. Furthermore, upwelling plays an important role in providing nutrients to HABs. After estimating nutrient fluxes(NO3-N, PO4-P, Si O3-Si) in the upwelling areas along a typical section(S07), the results showed that the nutrient uptake rate is the greatest at 10-20 m below euphotic zone, sustaining the ongoing presence of HABs. The uptake rate of PO4-P is the highest among dissolved inorganic nutrients. Therefore, upwelling is most likely the main source of PO4-P supply to HABs.

关键词： nutrient structure flux Taiwan Warm Current upwelling East China Sea

Progress in the research of GSH in cells

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Chinese Science Bulletin 2011年第28期56卷 3057-3063页

作者： ZHAO hongmei RUAN HaiHua li HongTao Tianjin Key Laboratory of Food Biotechnology College of Biotechnology and Food Science Tianjin University of Commerce Tianjin 300134China

Glutathione (GSH), γ-Glu-Cys-Gly, is one of the most abundant small non-protein thiol molecules in mammalian tissues, particu- larly in the liver. Although glutathione is present in thiol-reduced (GSH) and disulfide oxidized (GSSG) forms, the predominant form is GSH and its content can exceed 10 mmol/L in liver cells. As an important intracellular reductant, GSH has many biological functions in cells. Its major function is as an anti-oxidant as it can protect proteins from oxidation by reversible posttranslational modification (glutathionylation) and decrease reactive oxygen species-mediated damage. However, it does have numerous other functions, including to chelate metal irons; enhance the absorption of iron, selenium and calcium; participate in lipid and insulin metabolism; regulate cellular events such as gene expression, DNA and protein synthesis, cell proliferation and apoptosis, redox-dependent signal transduction pathways, cytokine production and the immune response; and control protein glutathionylation. Therefore, GSH plays important roles in cell survival and health, and an imbalance in the GSH level can lead to many diseases. In this review, we provide an overview of the function of GSH in mammalian cells and discuss future research of GSH.

关键词： GSH 细胞内哺乳动物细胞抗氧化剂信号转导通路谷胱甘肽蛋白质半胱氨酸

Efficacy of Yiqigubiao pill on chronic obstructive pulmonary disease in rats with the disease induced by lipopolysaccharide and cigarette-smoke fumigation

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Journal of Traditional Chinese Medicine 2020年第6期40卷 983-991页

作者： Jiang lin li Fengsen Sun Jianhui Huo Hairu li Xiaoqin li hongmei Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region Urumqi830000China Institute of Chinese Materia Medica China Academy of Chinese Medical SciencesBeijing 100700China

OBJECTIVE:To investigate the efficacy of Yiqigubiao pill(YQGB)on chronic obstructive pulmonary disease(COPD)in rats with the COPD induced by lipopolysaccharide(LPS)and cigarettesmoke ***:In this study,six groups of rats were set up,including control group,model group,positive control group(aminophylline)and YQGB(high,medium and low doses)*** injection of lipopolysaccharide(LPS)and cigarette-smoke fumigation induced COPD in *** general condition,incubation period and coughing times,lung function,level of inflammatory factors,leukocyte condition and pathological changes of bronchus and lung tissue were observed in rats of each ***:In the COPD rats,the latent period of coughing was shortened and the cough frequency was increased significantly;the pulmonary function was significantly decreased,which was manifested by the increased lung tissue resistance and respiratory system resistance,and the decreasing percentage of forced expiratory volume and forced expiratory volume in the 0.3 s(FEV0.3/FVC);the contents of tumor necrosis factor-alpha(TNF-α)and interleukin-4 in serum were obviously increased,and the NEUT%in bronchoalveolar lavage fluid was significantly *** could obviously prolong the latent period of cough,and reduce the cough frequency and the content of TNF-αin *** can also significantly reduce respiratory resistance and increase FEV0.3/FVC *** results of histopathology showed that YQGB significantly reduced the pathological changes of tracheal mucosa and lung caused by *** obviously increased level of AQP1,which was down-regulated in the COPD ***:YQGB could significantly improve the pulmonary function,reduce inflammation and alleviate lung and bronchial diseases in the COPD rats.

关键词： Pulmonary disease chronic obstructive Respiratory function tests Bronchoalveolar lavage fluid Inflammatory cytokines Yiqigubiao pill

p75NTR signal transduction suppressed by BFAR and p75NTR interactions

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Science China(life Sciences) 2012年第4期55卷 367-374页

作者： li hongmei SHI Huili HUO KeKe State Key Laboratory of Genetic Engineering School of Life Sciences Fudan University Shanghai 200433 China

p75NTR is a low-affinity nerve growth factor receptor, which promotes cell proliferation as a positive modulator of high-affinity receptor TrkA, as well as binds with cell ligands to induce apoptosis and mediate death signals. To analyze the regulatory mechanisms of p75NTR, the present study utilized a new membrane yeast two-hybrid system to screen a human fe- tal brain cDNA library. Results identified BFAR, a novel protein that interacts with p75NTR. Interaction specificity was veri- fied by membrane yeast two-hybrid co-transformation assays, in vitro GST pull-down assays, and in vitro co-irnmunopreci- pitation assays. The fluorescent subcellular localization assay revealed that the two proteins co-localized within the cytoplasm. BFAR overexpression in PC-12 and HEK293T cells inhibited the NFnB and JNK signaling pathway, as determined with the luciferase test. Co-transfected p75NTR and BFAR in HEK293T or PC-12 cells, respectively, increased the percentage of cells in the G2/M phase, decreased the number of S-phase cells, and did not change the number of G0/Gl-phase cells.

关键词： p75NTR BFAR membrane yeast two-hybrid protein-protein interaction apoptosis

Effect of acupuncture therapies combined with usual medical care on knee osteoarthritis

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Journal of Traditional Chinese Medicine 2019年第1期39卷 103-110页

作者： Zhang Lele Yuan Haixin Zhang Lei li Jian li hongmei The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and TechnologyLuoyang 471003China

OBJECTIVE: To observe the effect of acupuncture or electroacupuncture(EA) combined with usual medical care for treating knee osteoarthritis(KOA).METHODS: A total of 90 patients with KOA were randomly allocated to 3 groups: usual care group(UC group, n = 30) was treated by pharmacological treatment of non-steroidal anti-inflammatory drugs(NSAIDs) and drugs for activating blood circulation(Ds-ABC), acupuncture(AP) combined with usual care group(UC group)(AP + UC group, n =30) and EA combined with UC group(EA + UC group, n = 30). The primary outcome measurements included pain visual analogue scale/score(VAS), Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC Index) and its subscales. Secondary outcome measurement was Assessment of Quality of life instrument version of the 36-item Short Form Health Survey(AQoL-SF36).RESULTS: By the end of the 1 st week, AP + UC group and EA + UC group exhibited statistically significant improvements in primary outcome measures, except for WOMAC stiffness, compared with the UC group(P < 0.05). Moreover, the energy/fatigue domain of AQoL-SF36 in the AP + UC group showed better results than UC group(P < 0.05). By the end of the 2 nd week, all the primary outcome measures revealed that either the AP + UC or EA +UC group demonstrating remarkable advantages compared with the UC group(P < 0.05). The social functioning and general health domains of AQoLSF36 in the two acupuncture-intervention groups were improved significantly than UC group(P <0.05). We also found the energy/fatigue and emotional wellbeing domains of AQoL-SF36 in the EA +UC group demonstrated better results than UC group(P < 0.05).CONCLUSION: AP or EA combined with usual care is more effective than usual care alone for the treatment of KOA, the intervention of electric current in the process of acupuncture may improve more domains of AQoL-SF36 in KOA patients.

关键词： Osteoarthritis,knee Acupuncture Electroacupuncture Pain measurement

An estimation of nutrient fluxes to the East China Sea continental shelf from the Taiwan Strait and Kuroshio subsurface waters in summer

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Acta Oceanologica Sinica 2014年第11期33卷 1-10页

作者： li hongmei SHI Xiaoyong WANG Hao HAN Xiurong College of Chemistry and Chemical Engineering Ocean University of China National Marine Hazard Mitigation Service Fujian Marine Environment and Fishery Resource Monitoring Center

According to historical mean ocean current data through the field observations of the Taiwan Ocean Research Institute during 1991–2005 and survey data of nutrients on the continental shelf of the East China Sea（ECS） in the summer of 2006, nutrient fluxes from the Taiwan Strait and Kuroshio subsurface waters are estimated using a grid interpolation method, which both are the sources of the Taiwan Warm Current. The nutrient fluxes of the two water masses are also compared. The results show that phosphate（PO4-P）, silicate（SiO3-Si） and nitrate（NO3-N） fluxes to the ECS continental shelf from the Kuroshio upwelling water are slightly higher than those from the Taiwan Strait water in the summer of 2006. In contrast, owing to its lower velocity, the nutrient flux density（i.e., nutrient fluxes divided by the area of the specific section） of the Kuroshio subsurface water is lower than that of the Taiwan Strait water. In addition, the Taiwan Warm Current deep water, which is mainly constituted by the Kuroshio subsurface water, might directly reach the areas of high-frequency harmful alga blooms in the ECS.

关键词： nutrient flux Taiwan Warm Current summer East China Sea

Differential physiological responses of the coastal cyanobacterium Synechococcus sp. PCC7002 to elevated pCO2 at lag, exponential,and stationary growth phases

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Science China Earth Sciences 2018年第10期61卷 1397-1405页

作者： Shanli MOU Gang li hongmei li Fazhong li Zhisheng SHAO Jinsong li Changfeng QU Yongyu ZHANG Key Laboratory of Biofuels Shandong Provincial Key Laboratory of Energy Genetics Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of SciencesQingdao 266101China Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of SciencesGuangzhou 510301 China Zhanjiang Branch of China National Offshore Oil Corporation Ltd. Zhanjiang 524000 China Key Laboratory of Marine Bioactive Substance the First Institute of OceanographyState Oceanic AdministrationQingdao 266061 China

We studied the effects of expected end-of-the-century p CO_2(1000 ppm)on the photosynthetic performance of a coastal marine cyanobacterium Synechococcus ***7002 during the lag,exponential,and stationary growth *** p CO_2significantly stimulated growth,and enhanced the maximum cell density during the stationary *** ambient p CO_2conditions,the lag phase lasted for 6 days,while elevated p CO_2shortened the lag phase to two days and extended the exponential phase by four *** elevated p CO_2increased photosynthesis levels during the lag and exponential phases,but reduced them during the stationary ***,the elevated p CO_2reduced the saturated growth light(Ik)and increased the light utilization efficiency(α)during the exponential and stationary phases,and elevated the phycobilisome:chlorophyll a(Chl a)***,the elevated p CO_2reduced the particulate organic carbon(POC):Chl a and particulate organic nitrogen(PON):Chl a ratios during the lag and stationary phases,but enhanced them during the exponential ***,Synechococcus showed differential physiological responses to elevated p CO_2during different growth phases,thus providing insight into previous studies that focused on only the exponential phase,which may have biased the results relative to the effects of elevated p CO_2in ecology or aquaculture.

关键词： Elevated pCO2 Lag Exponential and stationary phases Photosynthetic performance Synechococcus

Isolation,identification and optimization of fermentation conditions against Sclerotinia sclerotiorum strains in high salt Doenjang

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Food Science and Human Wellness 2021年第2期10卷 205-213页

作者： Xian Zhang hongmei li Xiaoyu Kang Seongil lim Fanzhu li Agricultural College Yanbian UniversityYanjiJilinChina Fermentation and Functionality Research Group Korea Food Research InstituteKorea

With the global warming,the cultivation and storage of northern Chinese cabbage has been infected by Sclerotium bacteria,resulting in the whole plant *** this study,in order to develop a bio-inhibitory agent with antibacterial effect and no toxicity and no residue,three strains with high inhibition rate against Sclerotinia sclerotiorum were screened from 69 samples of Doenjang collected in different *** observing culture characteristics and physiological and biochemical tests,three strains were identified through 16S rDNA sequence and phylogenetic *** results showed that SP-6 was Bacillus amyloliquefaciens,SP-13 was Bacillus methylotrophicus,and SP-14 was Bacillus ***,the inhibition rate of the aseptic fermentation filtrate of three strains against *** was determined and the fermentation conditions of SP-14 strain was *** showed that all of three fermentation filtrates inhibited *** showed that the best conditions for fermentation for the antibacterial substances of SP-14 strain is as following:1%maltose,1%ammonium oxalate or beef extract,1%ferrous sulfate,fermentation at 32℃for 36 h,initial pH 8–9 and the inoculation amount 5%.The living bacteriostatic test showed that SP-14 strain had good inhibitory effect on *** and the inhibitory effect was more significant when the bacteria concentration was 1.2×10^(11)CFU/mL.

关键词： Doenjang Bacillus Sclerotinia sclerotiorum Antagonism

MOF synthesis using waste PET for applications of adsorption, catalysis and energy storage

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Green Energy & Environment 2024年第11期9卷 1650-1665页

作者： hongmei li Jinming Lei liying Zhu Yanling Yao Yuanhua li Tianhao li Chuntian Qiu College of Food and Bioengineering Chengdu UniversityChengdu610106China ZJU-Hangzhou Global Scientifc and Technological Innovation Center Zhejiang UniversityHangzhou311215China School of Chemistry and Materials Engineering Huizhou UniversityHuizhou516007China Chongqing Key Laboratory for Advanced Materials and Technologies of Clean Energy School for Materials and EnergySouthwest UniversityChongqing400715China Yibin Academy of Southwest University University RoadYibinSichuan644005China

Polyethylene terephthalate (PET) as one of non-degradable wastes has become a huge threat to the environment and human *** Recycle of PET is a sustainable way to release 1,4-benzenedicarboxylic acid (BDC) the monomer of PET as common used organiclinker for synthesis of functional Metal–organic-frameworks (PET-derived MOFs) such as UiO-66, MIL-101, etc. This sustainable and costeffective“Waste-to-MOFs” model is of great signiﬁcant to be intensively investigated in the past years. Attributes of substantial porosity, speciﬁcsurface area, exposed metal centers, uniform structure, and ﬂexible morphology render PET-derived MOFs are well-suited for applications inadsorption, energy storage, catalysis, among others. Herein, in the present work, we have summarized recent advances in synthesis of PETderived MOFs using ex-situ and in-situ methods for typical applications of adsorption, catalysis and energy storage. Despite those improvementsin synthesis methods and potential applications, challenges still remain in development of green and economical routes to fully utilize waste PETfor massive manufacture of valuable MOF materials and chemicals. This review provides insights into the conversion of non-degradable PETwaste to value-added MOF materials, and further suggests promising perspectives to develop the sustainable “Waste-to-MOFs” model inaddressing environmental pollution and energy crises.

关键词： Plastic waste Polyethylene terephthalate Chemical recycle 1,4-benzenedicarboxylic acid Metal organic frameworks