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Neferine inhibits the progression of diabetic nephropathy by modulating the miR-17-5p/nuclear factor E2-related factor 2 axis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 44-53页

作者： huang hongmei YANG Maojun LI Ting WANG Dandan LI Ying TANG Xiaochi YUAN Lu GU Shi XU Yong Department of Endocrinology Chengdu Shuangliu District First People's Hospital(West China Airport of Sichuan University)Chengdu 610200China Department of Endocrinology Affiliated Hospital of Southwest Medical UniversityLuzhou 646000China

OBJECTIVE:To investigate the effect of Neferine(Nef)on diabetic nephropathy(DN)and to explore the mechanism of Nef in DN based on miRNA regulation ***:A DN mouse model was constructed and treated with *** creatinine(Crea),blood urea(UREA)and urinary albumin were measured in mice by kits,and renal histopathological changes and fibrosis were observed by hematoxylin-eosin staining and Masson *** tissue superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)activities were measured by enzyme-linked immunosorbent assay(ELISA).Western blotting was used to detect the expression of nuclear factor E2-related factor 2(Nrf2)/heme oxygenase 1(HO-1)signaling pathway-related proteins in kidney *** reverse transcription-polymerase chain reaction(q RT-PCR)was used to detect the expression of miR-17-5p in kidney ***,a DN in vitro model was constructed by high glucose culture of human mesangial cells(HMCs),cells were transfected with miR-17-5p mimic and/or treated with Nef,and we used q RTPCR to detect cellular miR-17 expression,flow cytometry to detect apoptosis,ELISAs to detect cellular SOD,MDA,and GSH-Px activities,Western blots to detect Nrf2/HO-1 signaling pathway-related protein expression,and dual luciferase reporter gene assays to verify the targeting relationship between Nrf2 and ***:Administration of Nef significantly reduced the levels of blood glucose,Crea,and UREA and the expression of miR-17-5p,improved renal histopathology and fibrosis,significantly reduced MDA levels,elevated SOD and GSH-Px activities,and activated Nrf2 expression in kidney tissues from mice with ***2 is a post-transcriptional target of *** HMCs transfected with miR-17-5p mimics,the m RNA and protein levels of Nrf2 were significantly ***,miR-17-5p overexpression and Nef intervention resulted in a significant increase in high glucose-induced apoptosis and MDA levels in HMC

关键词： diabetic nephropathies neferine miR-17-5p NF-E2-related factor 2 oxidative stress

Landscape patterns and their evolutions in　the suburban ecotone──A case study of the eastern suburb of Beijing

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Journal of Environmental Sciences 1994年第3期6卷 277-286页

作者： Yang Jurong,Zhang hongmei,huang Yi Institute of Environmental Science,Beijing Normal University,Beijing 100875,China

The characteristics and evolutions of the landscape patterns in the suburban ecotone are studied on the basis of landscape-ecological maps of different years derived from the corresponding aerial photos The relationship between landscape patterns and urban economical development is ana- lyzed. The diversity index is used to characterize the features of the landscape patterns in the ecotone and define the location of Sharp change.

关键词： suburban ecotone landscape patterns diversity index.

STING mediates microglial pyroptosis via interaction with NLRP3 in cerebral ischaemic stroke

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Stroke & Vascular Neurology 2024年第2期9卷 153-164页

作者： Wenyu Li Nan Shen Lingqi Kong hongmei huang Xinyue Wang Yan Zhang Guoping Wang Pengfei Xu Wei Hu Department of Neurology The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhuiChina

background Ischaemia-evoked neuroinflammation is a critical pathogenic event following ischaemic *** D(GSDMD)-associated pyroptosis represents a type of inflammation-associated programmed cell death,which can exacerbate neuroinflammatory responses and brain *** of interferon genes(STING)was recently described as a vital innate immune adaptor protein associated with ***,the regulatory effects of STING on microglial pyroptosis post-stroke have not been well *** STING-knockout and wild-type(WT)mice were subjected to middle cerebral artery occlusion(MCAO).STING small interfering RNA(siRNA)was transfected into BV2 cells before oxygen-glucose deprivation/reoxygenation(OGD/R).STING-overexpressing adeno-associated virus(AAV)and NOD-like receptor family pyrin domain containing 3(NLRP3)siRNA were administered by stereotaxic injection.2,3,5-Triphenyl tetrazolium chloride(TTC)staining,TdT-mediated dUTP nick end labeling(TUNEL)staining,Fluoro-Jade C(FJC)staining,neurobehavioural tests,immunohistochemistry,cytokine antibody array assay,transmission electron microscopy,immunoblot,Enzyme-linked immunosorbent assay(ELISA)and quantitative real-time polymerase chain reaction(qRT-PCR)were carried ***-immunoprecipitation assays were used to investigate the interplay between STING and *** STING expression was increased after MCAO and mainly detected on *** deletion alleviated brain infarction,neuronal damage and neurobehavioural impairment in mice subjected to *** knockout suppressed microglial activation and the secretion of inflammatory chemokines,accompanied by mitigation of microglial *** upregulation of microglial STING by AAV-F4/80-STING aggravated brain injury and microglial ***,co-immunoprecipitation showed that STING bound to NLRP3 in *** of NLRP3 siRNA reversed AAV-F4/80-STING-induced deterioration of microglial pyropt

关键词： NLRP3 cerebral inflammation

Relationship between infection,physical and mental health and exercise habits of some Chinese residents after recovery from COVID-19

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Sports Medicine and Health Science 2024年第3期6卷 260-265页

作者： Aiyi Zhou Yuncan Xia Peng Pi Zhengzhen Wang hongmei huang Yan Wang School of Sports Medicine and Rehabilitation Beijing Sport UniversityBeijingChina ENNOVA Institute of Life Science and Technology Enn GroupLangfangHebei ProvinceChina

This study aimed to determine the infection status,exercise habits,anxiety levels,and sleep quality in Chinese residents who recovered from infection during the period of coronavirus disease 2019(COVID-19)*** also aimed to investigate the influencing factors of recovery status and aid in improving intervention measures for COVID-19 *** study is a sub-study nested within a cross-sectional investigation of infection and physical and mental health among partially recovered residents in all 34 provincial areas of China during the COVID-19 pandemic.A total of 1013 participants(374 males and 639 females)completed the *** endurance was significantly lower after infection than before infection(p<0.001).Women(3.924.97)exhibited higher levels of anxiety than men(3.334.54,p?0.015).The sleep score was significantly higher after infection(8.277.05)than before infection(4.174.97,p<0.001).The active and regular exercise groups exhibited significantly shorter durations of fever than the sedentary and irregular groups(p?0.033;p?0.021).Additionally,the active group demonstrated significantly fewer recovery days([7.323.24]days)than the sedentary group([7.663.06]days,p?0.035).We found a correlation between age and the recovery time of symptoms after *** noted that a greater number of symptoms corresponded to poorer cardiopulmonary fitness and sleep *** who engage in sedentary lifestyles and irregular exercise regimens generally require prolonged recovery ***,incorporating moderate exercise,psychological support,sleep hygiene and other health interventions into post-COVID-19 recovery measures is imperative.

关键词： COVID-19 infection Post-recovery of COVID-19 Recovery status Cardiopulmonary endurance Anxiety Sleep quality

Ionic Liquid-assisted Formation of Lanthanide Metal-organic Framework Nano/Microrods for Superefficient Removal of Congo Red

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Chemical Research in Chinese Universities 2015年第6期31卷 899-903页

作者： XIAO Yi CHEN Fang ZHU Xixi QIN Hongling huang hongmei ZHANG Youyu YIN Dulin HE Xiaoxiao WANG Kemin Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of EducationCollege of Chemistry and Chemical Engineering Hunan Normal University Changsha 410081 P. R. China State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry andChemical Engineering College of Biology Hunan University Changsha 410082 P. R. China

New lanthanide metal-organic framework（MOF） nano/microrods, [C4mim]Cl-Eu-MOF, [C8mim]Cl-Eu- MOF and [C12mim]Cl-Eu-MOF, were conveniently synthesized via an ionic liquid-assisted hydrothermal method and characterized by means of powder X-ray diffraction（XRD）, Fourier transform infrared spectroscopy（FTIR）, thermo- gravimetric analysis（TG） and transmission electron microscopy（TEM）. The obtained nano/microrods with low sur- face areas were efficient for the removal of Congo red（CR） from aqueous solutions. Under the optimum conditions, [C4mim]Cl-Eu-MOF with a specific surface area of 5.1 m^2/g exhibited an ultrahigh adsorption capacity of 2606 mg/g toward CR. Notably, the adsorption efficiency of [C4mim]Cl-Eu-MOF for CR via nano/microscale stacking can be directly demonstrated by TEM. In-depth understanding of CR removal by [C4mim]Cl-Eu-MOF nano/microrods was also supported by FTIR, Raman spectroscopy and zeta potential analyses.

关键词： Lanthanide metal-organic framework Nano/microrod Ionic liquid Congo red Adsorption

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基于在线学习RBF神经网络的故障预报

南京航空航天大学学报 2007年第2期39卷 249-252页

作者：黄红梅胡寿松南京航空航天大学自动化学院南京210016

提出了一种基于在线学习神经网络的故障预报方法。该方法在网络设计过程中结合了“添加”准则和基于对网络输出贡献相对较小的“剪枝”准则。“添加”过程中利用隐层的最大输出判断神经元的活跃性;“剪枝”过程中加入了滑动窗口,避免了... 详细信息

提出了一种基于在线学习神经网络的故障预报方法。该方法在网络设计过程中结合了“添加”准则和基于对网络输出贡献相对较小的“剪枝”准则。“添加”过程中利用隐层的最大输出判断神经元的活跃性;“剪枝”过程中加入了滑动窗口,避免了误“剪枝”。同时,调整过程只对输出响应比较大的神经元进行,大大减少了计算量,提高了实时性。仿真结果表明,利用该算法能够对一类带时变参数的非线性系统进行故障预报。

关键词：故障预报 RBF神经网络在线学习算法时变参数非线性系统

“大创”环境下高校信息素养培养模式优化研究

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图书馆建设 2019年第2期 142-147页

作者：黄红梅大连外国语大学图书馆参考咨询部

通过创新素养实践案例分析,定位大学生创新创业训练计划环境下高校信息素养培养模式,提出运用整体性思考方法培养学生创新思维、利用网络信息的运用和转化提升学生创新意识、依托系统方法提升学生创新能力、借助新媒体技术训练学生创新... 详细信息

通过创新素养实践案例分析,定位大学生创新创业训练计划环境下高校信息素养培养模式,提出运用整体性思考方法培养学生创新思维、利用网络信息的运用和转化提升学生创新意识、依托系统方法提升学生创新能力、借助新媒体技术训练学生创新技能的优化策略。

关键词：创新创业信息素养创新素养

Study on In vitro Regeneration System of Melon

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Agricultural Biotechnology 2012年第1期1卷 23-26页

作者： Yan ZHAO Qingbo LIU Chunmei REN Shanshan MA hongmei huang College of Bioscience and Biotechnology Hunan Agricultural University

[ Objective] This study aimed to optimize the in vitro regeneration system of melon. [ Method] Melon variety Nanxiang91023 was selected as experi- mental materials, with the cotyledons and hypocotyls as explants, different types and concentrations of growth regulators were supplemented at different stages of tis- sue culture, to explore the simple and effective medium formula for regeneration of melon. [ Result] MS ＋ 1.5 mg/L of 6-BA ＋0.2 mg/L of LAA was the optimal medium for induction and proliferation of callus; MS ＋ 1.0 mg/L of 6-BA ＋ 0. 2 mg/L of 2, 4-D was the optimal medium for differentiation of adventitious buds; MS ＋ 1.0 mg/L of ZT ＋ 0.2 mg/L of LAA was the optimal medium for rooting of seedlings. On the basis of above conditions, melon seedlings had high roofing rate and strong roots. [ Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.

关键词： Melon Regeneration system Hypocotyls Cotyledons

信息素养教学设计实践研究——由教育部高校图工委信息素养培训研讨会引发的思考

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图书馆学研究 2020年第15期 17-22页

作者：黄红梅大连外国语大学图书馆

2019年6月教育部高校图工委召开信息素养培训研讨会。研讨会22个案例充分展示信息素养教学设计实践情况,教学设计理念、教学设计方法和教学工具特征鲜明;22个案例同时揭示信息素养教学设计课程知识整合、思维与知识情景联结、外显知识... 详细信息

2019年6月教育部高校图工委召开信息素养培训研讨会。研讨会22个案例充分展示信息素养教学设计实践情况,教学设计理念、教学设计方法和教学工具特征鲜明;22个案例同时揭示信息素养教学设计课程知识整合、思维与知识情景联结、外显知识与内隐知识相结合的实践模式,为信息素养教学设计构建合理知识体系提供启发。

关键词：信息素养教学设计设计理念教学工具