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Effects of various frequency electrical stimulation of the dorsal raphe nucleus on spontaneous firing activities in the rat subthalamic nucleus

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Neural Regeneration Research 2008年第3期3卷 276-279页

作者： hongmei Ran Dongming gao Department of Physiology Liaoning Medical University Jinzhou 121001 Liaoning Province China

BACKGROUND： Some investigations have demonstrated that exogenous 5-hydroxytryptamine increases the spontaneous firing rate of subthalamic nucleus （STN） neurons in the rat brain. OBJECTIVE： To validate the effect of electrical stimulation to the dorsal raphe nucleus （DRN） on the neuronal activities of the STN in rats, as well as analyze the differences in the effects of electrical stimulation at various frequencies. DESIGN, TIME AND SETTING： Experiments were performed from March 2007 to June 2007 in the Electrophysiology Laboratory of Liaoning Medical University with a randomized controlled animal study design. MATERIALS： Twenty-four healthy male Sprague-Dawley （SD） rats, weighing 250-350 g, were selected for this study. An A320R constant electrical stimulator was purchased from World Precision Instruments Company （USA）; a Spike 2 biological signal acquisition system was purchased from British CED Company. METHODS： Twenty-four SD rats were randomly assigned into a model group and a normal group, with 12 rats in each group. To mimic Parkinson＇s disease, rats in the model group were injected with 4μL of 6-hydroxydopamine into the right striatum, then received deep brain stimulation. Rats in the normal group received deep brain stimulation in same brain region without modeling. Electrical stimulation （width, 0.06 ms; intensity, 0.2-0.6 mA; frequency, 20-130 Hz; train duration, 5 seconds） was delivered to the DRN. MAIN OUTCOME MEASURES： The firing rates of STN neurons were observed by extracellular recording using a biological signal acquisition system. RESULTS： DRN-high-frequency stimulation （DRN-HFS） induced excitation in 59% of the STN neurons in the normal group and 50% of the STN neurons in the model group; mean firing rates increased significantly from （7.14±0.75） and （7.94 ± 0.61） Hz to （11.17 ±1.49） and （12.11 ± 1.05） Hz, respectively （P 〈 0.01）. Spontaneous firing rate increased significantly in 53% of neurons in normal rats in a frequency-dependent manner when

关键词： subthalamic nucleus dorsal raphe nucleus electrical stimulation

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Evaluation of the effects of EPS composite soil replacement on the dynamic performance of caisson structure using shaking table tests

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Earthquake Engineering and Engineering Vibration 2024年第4期23卷 829-843页

作者： gao hongmei Ji Zhanpeng Zhang Xinlei Zhang Shushan Wang Zhihua Shen Guangming Research Center of Urban Underground Space Nanjing Tech UniversityNanjing 211816China Institute of Geotechnical Engineering Nanjing Tech UniversityNanjing 211816China

The seismic performance of a caisson structure under two types of models with a saturated sandy foundation(CSS)and an expanded polystyrene(EPS)composite soil foundation(CES)are studied using shaking table *** macro phenomena of the two different foundation models are described and *** effects of the replacement of EPS composite soil on seismic-induced liquefaction of backfill and the dynamic performance of a caisson structure are evaluated in *** results show that the excess pore water pressure generation in the CES is significantly slower than that in the CSS during the *** dynamic earth pressure acting on the caisson has a triangular *** response of horizontal acceleration,displacement,settlement,and rotation angle of the caisson in the CES is smaller than that in the CSS,which means the caisson in the CES has a better seismic ***,the out-of-phase phenomenon between dynamic earth thrust and inertial force in the CES is more obvious than that in the CSS,which is beneficial to reduce the lateral force and improve the stability of the caisson structure.

关键词： EPS composite soil foundation Caisson-type quay wall shaking table test phase difference rotation angle

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Creep behavior of EPS composite soil

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Science China(Technological Sciences) 2012年第11期55卷 3070-3080页

作者： gao hongmei CHEN YuMin LIU HanLong LIU JinYuan CHU Jian Institute of Geotechnical Engineering Nanjing University of Technology Nanjing 210009 China Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering Hohai University Nanjing 210098 China Geotechnical Research Institute Hohai University Nanjing 210098 China Department of Civil Engineering Ryerson University 350 Victoria Street Toronto ONM5B2k3 Canada School of Civil and Environmental Engineering Nanyang Technological University Singapore 639798 Singapore

EPS composite soil is one type of premixed lightweight fills studied by numerous researchers, However, one aspect that has not been fully understood is the creep behaviors which may have significant effect on the design and application of EPS com- posite soil. In this paper, the results of a series of oedometer creep tests and triaxial undrained creep tests on EPS composite soil were presented. Four main influencing factors were identified and their effects on the creep behaviors of EPS composite soil were studied. Three well established creep models, namely, Findley model, Singh ＆ Mitchell model, and Mesri model, were used to simulate the creep behavior of EPS composite soil. This study shows that the Findley creep model fits the test re- suits the best. A semi-empirical creep model was also proposed to model the creep behavior under axisymmetric conditions. In this model, the creep strain was divided into instant and viscous elastic strain as well as instant and viscous plastic strain which were simulated by element models and empirical equations, respectively. It was shown that the proposed creep model was able to precisely predict the creep strain of EPS composite soil.

关键词： lightweight fill creep model empirical model EPS composite soil oedometer creep test triaxial undrained creep test

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Curing mechanism of furan resin modified with different agents and their thermal strength

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China Foundry 2011年第2期8卷 161-165页

作者： Huang Renhe gao hongmei Tang Yaoji Liu Qingyun School of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao 26510 China

The curing mechanism of furfuryl alcohol and urea-formaldehyde furan resins was investigated using infrared spectroscopy(IR) *** curing productions of urea-formaldehyde furan resins modified with different agents(***,polyester polyol,phenol and acetone) and the productions of incomplete curing were characterized by differential thermal analysis(DTA) and thermal gravity analysis(TG).The results indicate that except for polyester polyol,the other modifiers have little effect on the thermal strength of urea-formaldehyde furan ***,the thermal strength can be improved at a temperature of higher than 550℃.

关键词： no-bake furan resin modification thermal gravity analysis (TG) curing mechanism thermal strength

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Oxygen-vacancy-rich TiO_(2)enables highly active and durable water electrolysis of urchin-like RuO_(2)catalyst

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Science China(Technological Sciences) 2022年第10期65卷 2317-2324页

作者： gao hongmei XIAO MeiLing LI GuoQiang gao LiQin MENG QingLei LUO ZhaoYan LUO ErGui LIU ChangPeng JIN Zhao GE JunJie XING Wei State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China Laboratory of Advanced Power Sources Jilin Province Key Laboratory of Low Carbon Chemical Power SourcesChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China.

The material innovation is prerequisite to accelerating sluggish oxygen evolution reaction(OER)kinetics,thus promoting the realization of hydrogen energy ***,we develop an oxygen-vacancy-rich TiO_(2)supported RuO_(2)catalyst(RuO_(2)@r-TiO_(2))towards improved OER activity and *** oxygen vacancy on TiO_(2)not only supplies electrons to produce lower valence Ru,but also provides sufficient anchoring site for the deposition of RuO_(2)*** that,it can generate strong electronic interaction between TiO_(2)and supported RuO_(2),and thereby tailors the intermediates’adsorption energy on the RuO_(2)*** a result,the derived RuO_(2)@r-TiO_(2)catalyst exhibits superior OER activity and stability with the overpotential of 211 mV at a current density of 10 mA cm^(−2)and negligible activity degradation after 6 h operation,outperforming the non-oxygen-vacancy counterpart(223.3 mV,12.75%activity loss)and RuO_(2)catalyst(234.6 mV,42.86%activity loss).

关键词： oxygen evolution reaction oxygen vacancy electronic interaction stability

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Expression of calbindin D28K in substantia nigra of model rats with Parkinson disease

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Neural Regeneration Research 2006年第2期1卷 121-125页

作者： Dianshuai gao hongmei Liu Yanxia Ding Hongjun Wang Yanqiang Wang Department of Neurobiology Xuzhou Medical CollegeXuzhou 221002 JiangsuProvince China

BACKGROUND： Previous researches suggested that expression level of calbindin D28K mRNA decreased in substantia nigra （SN） of model rats with Parkinson disease （PD）, and this might be related to the decrease of anti-degeneration potentials of dopaminergic neurons. OBJECTIVE： To observe expression changes of calbindin D28K in SN dopaminergic neurons during their degeneration and death in midbrain of PD model rats. DESIGN： Completely randomized grouping design SETTING： Department of Neurobiology, Xuzhou Medical College MATERIALS＂ A total of 92 healthy male SD rats, with the age of 3 months, weighing 200-250 g, were selected from Experimental Animal Center of Xuzhou Medical College [certification： SCXK （su） 2003-0003]. Calbindin D28K（CB）, tyroxine hydroxylase （TH）, ABC kit, 6-hydroxydopamine （6-OHDA） and Nissl dyes were provided by Sigma Company, and sheep serum was provided by Beijing Zhongshan Company. METHODS： The experiment was carried out in the Neurobiological Center of Xuzhou Medical College from October 2003 to October 2004. ① With lot method, rats were divided into blank control group （n=28）, experimental control group （n=-28） and experimental group （n=36）. Rats in experimental group were injected with 6-OHDA at right corpus striatum for PD modeling; rats in experimental control group were injected with saline at the same site; rats in blank control group did not give any injections.② On the 7^th, 14^th, 21^st and 28^th days, SN segments on right midbrain from every 5 rats in experimental group were fixed, embedded with paraffin and cut into successively coronary pieces. Rats in other two groups were treated with the same methods and then stained with Nissl to show neuronal form. Meanwhile, CB and TH antibodies staining with immunohistochemistry were used to show CB containing dopaminergic neurons and dopaminergic neurons, and cells were calculated and observed under optic microscope. ③ On the 14^th and 28^th days, every 4 rats in experimental group and eve

关键词： Figure Expression of calbindin D28K in substantia nigra of model rats with Parkinson disease

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Effects of ex situ conservation on diversity and function of the gut microbiota of the Tibetan wild ass(Equus kiang)

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Integrative Zoology 2023年第6期18卷 1089-1104页

作者： hongmei gao Feng JIANG Jingjie ZHANG Xiangwen CHI Pengfei SONG Bin LI Zhenyuan CAI Tongzuo ZHANG Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina Qinghai Provincial Key Laboratory of Animal Ecological Genomics XiningChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina Department of Student Affairs Qinghai UniversityXiningChina

Ex situ conservation is the main method for the protection of endangered *** explore the effect of ex situ conservation on the gut microbiota of the kiang(Equus kiang),metagenomic sequencing combined with bioinformatics analysis was used to investigate the composition and function of the gut microbiota of the *** results showed that ex situ conservation not only protected wildlife,but also affected the composition and function of gut microbiota,as well as the health of *** the zoo,the ratio of the relative abundance of Firmicutes to that of Bacteroidetes(F/B)is higher,clusters of potentially pathogenic bacteria(such as Catonella,Catonella,and Mycoplasma)are more numerous,the abundance of resistance genes is higher,and the abundance of metabolic functions is *** dynamic changes of the gut microbiota also played an important role in the nutritional absorption,energy metabolism,and environmental adaptation of the *** the rearing environment and increasing food diversity play important roles for increasing the diversity of gut microbiota,reducing the spread of potentially pathogenic bacteria,and reducing *** the wild,especially in winter and in food-deficient areas,food supplementation can enhance the gut microbial homeostasis of wild animals and reduce the impact of *** depth studies of the gut microbial function of wildlife have important implications for improving ex situ conservation.

关键词： ex situ conservation metagenome potential pathogenic bacteria resistance genes Tibetan wild ass

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Estimation of inbreeding and identification of regions under heavy selection based on runs of homozygosity in a Large White pig population

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Journal of Animal Science and Biotechnology 2020年第4期11卷 965-974页

作者： Liangyu Shi Ligang Wang Jiaxin Liu Tianyu Deng Hua Yan Longchao Zhang Xin Liu hongmei gao Xinhua Hou Lixian Wang Fuping Zhao Key Laboratory of Animal Genetics Breeding and Reproduction(poultry)of Ministry of AgricutureInstitute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China

Background: Runs of homozygosity(ROHs) are homozygous segments of the genome where the two haplotypes inherited from the parents are identical. The current availability of genotypes for a very large number of single nucleotide polymorphisms(SNPs) is leading to more accurate characterization of ROHs in the whole genome. Here,we investigated the occurrence and distribution of ROHs in 3,692 Large White pigs and compared estimates of inbreeding coefficients calculated based on ROHs(FROH), homozygosity(FHOM), genomic relationship matrix(FGRM)and pedigree(FPED). Furthermore, we identified genomic regions with high ROH frequencies and annotated their candidate ***: In total, 176,182 ROHs were identified from 3,569 animals, and all individuals displayed at least one ROH longer than 1 Mb. The ROHs identified were unevenly distributed on the autosomes. The highest and lowest coverages of Sus scrofa chromosomes(SSC) by ROH were on SSC14 and SSC13, respectively. The highest pairwise correlation among the different inbreeding coefficient estimates was 0.95 between FROH_totaland FHOM, while the lowest was-0.083 between FGRMand FPED. The correlations between FPEDand FROHusing four classes of ROH lengths ranged from 0.18 to 0.37 and increased with increasing ROH length, except for ROH > 10 Mb. Twelve ROH islands were located on four chromosomes(SSC1, 4, 6 and 14). These ROH islands harboured genes associated with reproduction, muscular development, fat deposition and adaptation, such as SIRT1, MYPN, SETDB1 and ***: FROHcan be used to accurately assess individual inbreeding levels compared to other inbreeding coefficient estimators. In the absence of pedigree records, FROHcan provide an alternative to inbreeding *** findings can be used not only to effectively increase the response to selection by appropriately managing the rate of inbreeding and minimizing the negative effects of inbreeding depression but also to help detect genomic regions with an

关键词： Candidate genes Inbreeding coefficients Runs of homozygosity Sus scrofa

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Genome-wide identification of RNA editing in seven porcine tissues by matched DNA and RNA high-throughput sequencing

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Journal of Animal Science and Biotechnology 2019年第2期10卷 339-352页

作者： Yuebo Zhang Longchao Zhang Jingwei Yue Xia Wei Ligang Wang Xin Liu hongmei gao Xinhua Hou Fuping Zhao Hua Yan Lixian Wang Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction Ministry of Agriculture Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China

Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still ***: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue ***, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the ***: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine.

关键词： ADAR A-to-G High-throughput sequencing RNA editing Swine

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Geochemical characteristics of Early Cretaceous source rocks in Boli Basin

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Global Geology 2006年第1期9卷 49-53,79页

作者： hongmei gao Fuhong gao Fu Fan Yueqiao Zhang College of Earth Sciences Jilin University Changchun 130061 Jilin China Research Institute of Petroleum Exploration and Development Beijing 100083 China

The Early Cretaceous deposits are composed of important source rocks in Boli Basin. The types of the source rocks include black mudstones and coal (with carbonaceous mudstone). By the organic geochemical analysis methods, the authors discussed the organic petrological characters, abundance of organic matter, degree of maturity and the type of source rocks. The main micro-component of black mudstone is exinite or vitrinite, and the content of vitrinite is high in coal. The weathering of the outcrop is very serious. The abundance of organic matter in source rock reaches the poor to better rank. The major kerogens in mudstone are type-Ⅲ, type-Ⅱ 2 and some type-Ⅱ 1; the organic type of coal is type-Ⅲ. The thermal evolution of the source rocks in every structural unit is very different, from low-maturity to over-maturity. The depositional environment is reductive, which is good for the preservation of organic matter. The organic matter in source rocks is mainly from aquatic organisms and terrigenous input.

关键词： source rock micro-component organic geochemistry weathering

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