检索结果-南通市图书馆

Sodium butyrate activates HMGCS2 to promote ketone body production through SIRT5-mediated desuccinylation

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2023年第2期17卷 339-351页

作者： Yanhong Xu Xiaotong Ye Yang Zhou Xinyu Cao Shiqiao Peng Yue Peng Xiaoying Zhang Yili Sun Haowen Jiang Wenying Huang hongkai lian Jiajun Yang Jia Li Jianping Ye Neurology Department Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 201306China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Shanghai Diabetes Institute Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai 200233China School of Physical Education Jiangxi Normal UniversityNanchang 330022China Metabolic Disease Research Center Zhengzhou University Affiliated Zhengzhou Central HospitalZhengzhou 450007China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Center for Advanced Medicine College of MedicineZhengzhou UniversityZhengzhou 450007China

Ketone bodies have beneficial metabolic activities,and the induction of plasma ketone bodies is a health promotion strategy.Dietary supplementation of sodium butyrate(SB)is an effective approach in the induction of plasma ketone bodies.However,the cellular and molecular mechanisms are unknown.In this study,SB was found to enhance the catalytic activity of 3-hydroxy-3-methylglutaryl-CoA synthase 2(HMGCS2),a rate-limiting enzyme in ketogenesis,to promote ketone body production in hepatocytes.SB administrated by gavage or intraperitoneal injection significantly induced bloodβ-hydroxybutyrate(BHB)in mice.BHB production was induced in the primary hepatocytes by SB.Protein succinylation was altered by SB in the liver tissues with down-regulation in 58 proteins and up-regulation in 26 proteins in the proteomics analysis.However,the alteration was mostly observed in mitochondrial proteins with 41%down-and 65%up-regulation,respectively.Succinylation status of HMGCS2 protein was altered by a reduction at two sites(K221 and K358)without a change in the protein level.The SB effect was significantly reduced by a SIRT5 inhibitor and in Sirt5-KO mice.The data suggests that SB activated HMGCS2 through SIRT5-mediated desuccinylation for ketone body production by the liver.The effect was not associated with an elevation in NAD+/NADH ratio according to our metabolomics analysis.The data provide a novel molecular mechanism for SB activity in the induction of ketone body production.

关键词： sodium butyrate succinylation HMGCS2 ketogenesis SIRT5

人口老龄化背景下某市医疗机构建筑无障碍水平调查研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

现代医院 2024年第2期24卷 211-214页

作者：连鸿凯侯战旗王建明周钰棨郑州大学附属郑州中心医院河南郑州450007

目的为当前老龄化时代和改善老年人的无障碍就医环境提供科学参考。方法2022年12月—2023年2月期间采用随机抽样法,抽取某市10家医疗机构的375名人员作为调查对象,发放自制的《医疗机构患者就医感受综合评分表(无障碍设施类)》调查问卷... 详细信息

目的为当前老龄化时代和改善老年人的无障碍就医环境提供科学参考。方法2022年12月—2023年2月期间采用随机抽样法,抽取某市10家医疗机构的375名人员作为调查对象,发放自制的《医疗机构患者就医感受综合评分表(无障碍设施类)》调查问卷375份,获得有效问卷356份并进行了信效度检验。问卷设定6个无障碍水平调查指标,每个指标设1至10分,对建筑无障碍水平进行问卷调查,收集的资料采用数据秩和比法和四分图模型进行统计分析。结果某市医疗机构的建筑无障碍平均得分为35.86分,多个指标处于中等水平,提示无障碍水平有待进一步提高。同时,不同医疗机构的经济运营状况对无障碍建设有一定影响,无障碍建设还与当地政府建设规划、工作人员的无障碍建筑意识等因素有关。结论为了应对老龄化挑战,提高老年人就医质量,降低社保成本,某市医疗机构可采取停车位、厕所等方面的无障碍改造,提升无障碍AI技术应用,打造智慧化就医场景等措施;政府需加快完善配套政策法规,加大无障碍设施改造方面的支持力度;高等院校、科研院所等可与医疗机构协同创新,产、学、研、用一体化发展,促进成果转化。

关键词：老年人抽样调查无障碍建筑数据秩和比四分图模型社保成本

Revisiting thermal comfort and thermal sensation

在线全文

学校读者我要写书评

暂无评论

Building Simulation 2024年第2期17卷 185-188页

作者： Zhiwei lian Department of Architecture Shanghai Jiao Tong UniversityShanghai200240China

The thermal environment is closely intertwined with comfort,health,efficiency,and building energy consumption.Thermal Comfort stands as a crucial concept for evaluating the thermal environment,yet the prevailing evaluation metric is Thermal Sensation.The correspondence between genuine Thermal Comfort and Thermal Sensation is influenced by a variety of environmental factors.

关键词： thermal Comfort prevailing

Modeling and Simulation of Lithium Vacuum Evaporation Process Using COMSOL Multiphysics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Thermal Science 2024年第1期33卷 86-100页

作者： lian Xuexin ZHONG Dawen Beijing Key Laboratory of Passive Nuclear Power Safety and Technology North China Electric Power UniversityBeijing 102206China

Based on the COMSOL Multiphysics simulation software,this study carried out modeling and numerical simulation for the evaporation process of liquid metal lithium in the vacuum free molecular flow state.The motion of lithium atoms in the evaporation process was analyzed through a succession of studies.Based on the available experimental values of the saturated vapor pressure of liquid metal lithium,the relationship between saturated vapor pressure and temperature of liquid lithium in the range of 600 K-900 K was obtained.A two-dimensional symmetric model(3.5 mm×20 mm) was established to simulate the transient evaporation process of liquid lithium at wall temperatures of 750 K,780 K,800 K,810 K,825 K,and 850 K,respectively.The effects of temperature,the evaporation coefficient,back pressure,and length-to-diameter ratio on the evaporation process were studied;the variation trends and reasons of the molecular flux and the pressure during the evaporation process were analyzed.At the same time,the evaporation process under variable wall temperature conditions was simulated.This research made the evaporation process of liquid lithium in vacuum molecular flow clearer,and provided theoretical support for the space reactor and nuclear fusion related fields.

关键词： free molecular flow saturated vapor pressure vacuum evaporation liquid lithium metal

Contrasting physical mechanisms of yellowfin tuna fluctuations between the western and eastern Indian Ocean

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2024年第3期42卷 960-971页

作者： Peng lian Le GAO Key Laboratory of Ocean Observation and Forecasting and Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Ocean Dynamics and Climate Laoshan LaboratoryQingdao 266061China

As an economically critical pelagic migratory species,yellowfin tuna(Thunnus albacores,YFT)is very sensible to physical and environmental conditions,such as sea surface temperature(SST),ocean heat content(OHC),and the mixed layer depth(MLD).We investigated the impact of SST,OHC,and MLD on fluctuations of YFT catch in the western/eastern Indian Ocean using the long time series of 63-year environmental and YFT datasets.We found that the impact of SST on YFT was heavily overestimated in the past,and MLD plays a more critical role in the YFT catch fluctuation.When the MLD deepens(>34.8 m),SST was more influential in predicting the catches of YFT than OHC in the western Indian Ocean,and OHC was more critical to YFT than SST in the eastern Indian Ocean.However,when the MLD shallows(<34.8 m),MLD was more vital to predict the catch per unit effort(CPUE)of YFT than SST/OHC in the western.After 2000,there was an asynchronous pattern of YFT CPUE induced by higher frequency variations and ocean hiatus of SST/OHC signals in the western and eastern Indian Oceans basins.The impact of the subsurface hiatus may induce the decrease of YFT in the eastern Indian Ocean.The above findings clarified a non-stationary relationship between the environmental factors and catches of YFT and provided new insights into variations in YFT abundance.

关键词： yellowfin tuna Indian Ocean sea surface temperature(SST) ocean heat content(OHC) mixed layer depth(MLD) hiatus

Impacts of central-Pacific El Niño and physical drivers on eastern Pacific bigeye tuna

在线全文

学校读者我要写书评

暂无评论

Journal of Oceanology and Limnology 2024年第3期42卷 972-987页

Bigeye tuna Thunnus obesus is an important migratory species that forages deeply,and El Niño events highly influence its distribution in the eastern Pacific Ocean.While sea surface temperature is widely recognized as the main factor affecting bigeye tuna(BET)distribution during El Niño events,the roles of different types of El Niño and subsurface oceanic signals,such as ocean heat content and mixed layer depth,remain unclear.We conducted A spatial-temporal analysis to investigate the relationship among BET distribution,El Niño events,and the underlying oceanic signals to address this knowledge gap.We used monthly purse seine fisheries data of BET in the eastern tropical Pacific Ocean(ETPO)from 1994 to 2012 and extracted the central-Pacific El Niño(CPEN)indices based on Niño 3 and Niño 4indexes.Furthermore,we employed Explainable Artificial Intelligence(XAI)models to identify the main patterns and feature importance of the six environmental variables and used information flow analysis to determine the causality between the selected factors and BET distribution.Finally,we analyzed Argo datasets to calculate the vertical,horizontal,and zonal mean temperature differences during CPEN and normal years to clarify the oceanic thermodynamic structure differences between the two types of years.Our findings reveal that BET distribution during the CPEN years is mainly driven by advection feedback of subsurface warmer thermal signals and vertically warmer habitats in the CPEN domain area,especially in high-yield fishing areas.The high frequency of CPEN events will likely lead to the westward shift of fisheries centers.

关键词： bigeye tuna central-Pacific El Niño(CPEN) ocean heat content Argo explainable artificial intelligence(XAI) information flow

Emerging materials and transistors for integrated circuits

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第3期11卷 7-8页

作者： Ming Liu lian-Mao Peng Lab of Nanofabrication and Novel Device Integration Institute of Microelectronics Chinese Academy of Sciences Key Laboratory for the Physics and Chemistry of Nanodevices and Center for Carbon-based Electronics School of Electronics Peking University

The device technology for integrated circuits(ICs) has received a great deal of attention in the past decade since the mainstream silicon-based complementary metal-oxide-semiconductor(CMOS) field-effect transistor(FET) for modern ICs had been encountering development bottlenecks due to the combination of power constraints, physics, cost and fabrication processes.

关键词：

Succinylation modification:a potential therapeutic target in stroke

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第4期19卷 781-787页

作者： Jie lian Wenwu Liu Qin Hu Xiaohua Zhang Department of Neurosurgery Ren Ji HospitalShanghai Jiao Tong University School of Medicine Department of Diving and Hyperbaric Medicine Naval Medical CenterNaval Medical University

Stroke is a leading cause of mortality and disability worldwide.Ischemic cell death triggered by the compromised supply of blood oxygen and glucose is one of the major pathophysiology of strokeinduced brain injury.Impaired mitochondrial energy metabolism is observed minutes after stroke and is closely associated with the progression of neuropathology.Recently,a new type of posttranslational modification,known as lysine succinylation,has been recognized to play a significant role in mitochondrial energy metabolism after ischemia.However,the role of succinylation modification in cell metabolism after stroke and its regulation are not well understood.We aimed to review the effects of succinylation on energy metabolism,reactive oxygen species generation,and neuroinflammation,as well as Sirtuin 5 mediated desuccinylation after stroke.We also highlight the potential of targeting succinylation/desuccinylation as a promising strategy for the treatment of stroke.The succinylation level is dynamically regulated by the nonenzymatic or enzymatic transfer of a succinyl group to a protein on lysine residues and the removal of succinyl catalyzed by desuccinylases.Mounting evidence has suggested that succinylation can regulate the metabolic pathway through modulating the activity or stability of metabolic enzymes.Sirtuins,especially Sirtuin 5,are characterized for their desuccinylation activity and have been recognized as a critical regulator of metabolism through desuccinylating numerous metabolic enzymes.Imbalance between succinylation and desuccinylation has been implicated in the pathophysiology of stroke.Pharmacological agents that enhance the activity of Sirtuin 5 have been employed to promote desuccinylation and improve mitochondrial metabolism,and neuroprotective effects of these agents have been observed in experimental stroke studies.However,their therapeutic efficacy in stroke patients should be validated.

关键词： mitochondria metabolism neuroprotection sirtuin 5 stroke succinylation modification

Prediction of cell-cell communication patierns of dorsal root ganglion cells: single-cell RNA sequencing data analysis

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2024年第6期19卷 1367-1374页

作者： Yanna lian Cheng Wu Li Liu Xiangyao Li National Health Commission and Chinese Academy of Medical Sciences Key Laboratory of Medical Neurobiology Ministry of Education Frontier Science Center for Brain Research and Brain-Machine Integration School of Brain Science and Brain Medicine Zhejiang University International Institutes of Medicine the Fourth Affiliated Hospital of Zhejiang University School of Medicine Zhejiang University-University of Edinburgh Institute Zhejiang University School of Medicine Zhejiang University 4. Biomedical Sciences College of Medicine and Veterinary Medicine The University of Edinburgh Core Facilities of the School of Medicine Zhejiang University

Dorsal root ganglion neurons transmit peripheral somatic information to the central nervous system, and dorsal root ganglion neuron excitability affects pain perception. Dorsal root ganglion stimulation is a new approach for managing pain sensation. Knowledge of the cell-cell communication among dorsal root ganglion cells may help in the development of new pain and itch management strategies. Here, we used the single-cell RNA-sequencing(scRNA-seq) database to investigate intercellular communication networks among dorsal root ganglion cells. We collected scRNA-seq data from six samples from three studies, yielding data on a total of 17,766 cells. Based on genetic profiles, we identified satellite glial cells, Schwann cells, neurons, vascular endothelial cells, immune cells, fibroblasts, and vascular smooth muscle cells. Further analysis revealed that eight types of dorsal root ganglion neurons mediated proprioceptive, itch, touch, mechanical, heat, and cold sensations. Moreover, we predicted several distinct forms of intercellular communication among dorsal root ganglion cells, including cell-cell contact, secreted signals, extracellular matrix, and neurotransmitter-mediated signals. The data mining predicted that Mrgpra3-positive neurons robustly express the genes encoding the adenosine Adora2b(A2B) receptor and glial cell line-derived neurotrophic factor family receptor alpha 1(GFRα-1). Our immunohistochemistry results confirmed the coexpression of the A2B receptor and GFRα-1. Intrathecal injection of the A2B receptor antagonist PSB-603 effectively prevented histamine-induced scratching behaviour in a dose-dependent manner. Our results demonstrate the involvement of the A2B receptor in the modulation of itch sensation. Furthermore, our findings provide insight into dorsal root ganglion cell-cell communication patterns and mechanisms. Our results should contribute to the development of new strategies for the regulation of dorsal root ganglion excitability.

关键词： cell-cell communications CellChat dorsal root ganglion scRNA-Seq database