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Effects of biochar produced from distiller grains on agronomic performances of sorghum(Sorghum bicolor L.) and greenhouse gas emissions from soil

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Pedosphere 2024年第5期34卷 951-959页

作者： hongjun YAng Suyi ZHAng Jingyu HU Jianguo HUAng Zonghua AO Xiaoqin WAng Zhe LI Xuepin LIAO College of Resources and Environment Southwest University National Engineering Research Center of Solid-State Brewing Luzhou Laojiao Co.Ltd. College of Biomass Science and Engineering Sichuan University

Aromatic liquor breweries produce massive distiller grains containing a high percentage of rice husks that necessitate harmless treatment and resource utilization. These husk-rich distiller grains can be pyrolyzed in the Ni-based catalyst system at a relatively low temperature（480?C） into combustible gas,which is used in liquor distillation, and biochar（BDG） with high mineral nutrients and good surface properties. A 3-year field experiment（2018–2020） was established to understand the effects of BDG on sorghum agronomic performances and greenhouse gas emissions from the soil. The results showed that BDG had higher mineral nutrient（N, P, K, and S） contents, larger cation exch.nge capacity, and better surface structure than those prepared using the traditional method at 400 and 600?C. Compared with sole chemical fertilizer（CF）, the combination of CF and BDG（CF+BDG） increased sorghum nutrient（N, P, and K） uptake, yield, fertilizer use efficiency, and economic benefit. Cumulative CO2emission from the soil ch.nged little between with and without BDG,indicating the microbial stability of BDG. The effective adsorption of NH3or NH4+by BDG upon N application may reduce N loss through NH3and N2O emissions and increase the efficiency of fertilizer N use. Cumulative ch4emission ranged from 32.45 to 44.86 g ha-1, which could be overlooked as a greenhouse gas in the sorghum field. Moreover, CF+BDG significantly decreased NH3and N2O emissions for the production of each unit of sorghum grains and the CO2emission from the land for the production of a certain amount of sorghum grains. Therefore, CF+BDG exhibited better agronomic and environmental performances in sorghum cultivation.

关键词： CO2 emission economic benefit environmental performance fertilizer use efficiency N2O emission NH3 emission nutrient uptake yield

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The adsorption and dissociation of multilayer ch_3OH on TiO_2(110)

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Science china chemistry 2015年第4期58卷 614-619页

作者： Ruimin Wang hongjun Fan State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical Physics

The adsorption and dissociation of ch3OH on TiO2 （110） were studied using density functional theory methods. Our results suggest that ch3OH molecules can adsorb up to 3/4 ML （1 ML=5.2× 10^14 molecules/cm2） coverage at five-coordinated titanium （Tisc） sites to form the first layer. In the second layer, the ch3OH is adsorbed at bridge-bonded oxygen, and from the third layer, the ch3OH molecules form a hydrogen-bonded network with each other. The theoretical results show that dissociation of multilayer adsorbed methanol to aldehyde occurs through a stepwise pathway, with easy O-H bond dissociation and rate-determining C-H bond dissociation. The dissociation barriers for 8 or 12 ch3OH molecules on TiO2 are higher than that for low coverage by 0.15-0.21 eV; this suggests that the dissociation of multilayer adsorbed ch3OH is harder.

关键词： adsorption dissociation multilayer ch3OH TiO2

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基于外膜蛋白的鼻气管鸟杆菌抗体间接ELISA检测方法的建立

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中国畜牧兽医 2024年第10期51卷 4420-4428页

作者：徐浩钧刘颖梅晨徐彤利凯王宏俊北京市农林科学院畜牧兽医研究所北京100097 河北北方学院张家口075000

【目的】建立鼻气管鸟杆菌(Ornithobacterium rhinotracheale,ORT)血清抗体的ELISA检测方法。【方法】利用原核表达方法克隆表达鼻气管鸟杆菌外膜蛋白OR02,经Western blotting鉴定其免疫原性。以纯化产物作为抗原包被于固相载体,采用棋... 详细信息

【目的】建立鼻气管鸟杆菌(Ornithobacterium rhinotracheale,ORT)血清抗体的ELISA检测方法。【方法】利用原核表达方法克隆表达鼻气管鸟杆菌外膜蛋白OR02,经Western blotting鉴定其免疫原性。以纯化产物作为抗原包被于固相载体,采用棋盘滴定法确定抗原包被浓度和血清稀释度。同时,优化ELISA反应条件,利用鼻气管鸟杆菌阴性血清确定该检测方法的阴阳临界值判定标准。通过检测其他病原阳性血清评估该方法的特异性,检测不同比例稀释的鼻气管鸟杆菌阳性血清以评估其灵敏性,并与商品化试剂盒进行比较,评估该ELISA方法的符合率。【结果】重组蛋白OR02以包涵体形式表达,分子质量约为58 ku,与预期相符。Western blotting显示,OR02重组蛋白能与鼻气管鸟杆菌阳性血清发生特异性结合,具有良好的免疫反应性。以纯化的OR02蛋白作为包被抗原,确定最佳抗原包被浓度为2 ng/μL,血清样本的稀释度为1∶50,孵育时间为30 min,酶标二抗的稀释度为1∶5000,孵育时间为30 min,最佳底物显色条件为在避光条件下孵育15 min。阴阳临界值的判定标准为:当待检血清D_(450 nm)值≥0.266时判定为阳性,当待检血清D_(450 nm)值<0.266时判定为阴性。用该方法检测副鸡禽杆菌(Apg)、新城疫病毒(NDV)、鸡传染性法氏囊病毒(IBDV)等单因子阳性鸡血清均无交叉反应。将抗体滴度为1∶64的鼻气管鸟杆菌阳性鸡血清以1∶800稀释仍能检测到阳性结果。与商业试剂盒对相同临床样本的检测结果的符合率为91.25%。【结论】本研究利用重组OR02蛋白建立了一种检测鼻气管鸟杆菌血清抗体的间接ELISA方法,该方法具有良好的特异性和灵敏性,且与商业试剂盒的符合率较高,可作为鸡血清鼻气管鸟杆菌抗体的检测工具。

关键词：鼻气管鸟杆菌外膜蛋白间接ELISA 特异性灵敏性

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Aspen Adsorption在气体吸附过程模拟方面的应用

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化学工业与工程 2024年第2期41卷 86-93页

作者：张妍李洪峻董志明杨丽程景才杨超中国科学院过程工程研究所石油化工分子转化与反应工程全国重点实验室北京100190 中国科学院大学化学工程学院北京100049 32181部队西安710032

Aspen Adsorption是一款吸附模拟软件,常用于气体吸附分离过程的模拟计算,对操作条件优化、设计优化、吸附材料性能评价等方面具有一定的指导作用。简要介绍了Aspen Adsorption软件的应用、模型参数和各种衡算方程,总结了其在穿透曲线... 详细信息

Aspen Adsorption是一款吸附模拟软件,常用于气体吸附分离过程的模拟计算,对操作条件优化、设计优化、吸附材料性能评价等方面具有一定的指导作用。简要介绍了Aspen Adsorption软件的应用、模型参数和各种衡算方程,总结了其在穿透曲线计算方面的模拟计算和应用,归纳了利用循环控制器Cycle Organizer针对各种类型的变压吸附PSA(Pressure Swing Adsorption)和变温吸附TSA(Temperature Swing Adsorption)过程进行模拟的情况。此外,针对Aspen Adsorption与其他软件结合使用的案例也进行了简要介绍。最后对Aspen Adsorption的未来发展进行了展望。

关键词：吸附 Aspen Adsorption 模拟应用

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The total column densities and fractional abundances of ch_3CN,ch_3OH,HCOOch_3 and^(13)CS in W51 cores

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Science china(Physics,Mechanics & Astronomy) 2005年第2期48卷 158-167页

作者： PEI chunchuan MA hongjun ZEng Qin LI Bo Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China

We present interferometric observations of the W51 region at 2.7 mm and 3.3 mm with the Berkeley-Illinois-Maryland Association Array. The lines of ch3CN (J = 6 – 5, K = 0,1,2,3,4), ch3OH (15-2 ?151 E), HCOOch3-A (71,6 – 61,5), HCOOch3 -E (71,6 – 61,5) and 13CS (2-1) as well as the 2.7 mm continuum toward W51 have been observed. The derived total beam-averaged column densities NT and fractional abundances of ch3CN, ch3OH, HCOOch3 and 13CS in W51 cores have been obtained.

关键词： massive star formation, interstellar molecular lines, column density, fractional abundance.

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Distribution of platelet-derived growth factor-alpha receptor expressing oligodendrocyte precursor cells in the adult rat brain

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Neural Regeneration Research 2009年第12期4卷 1093-1098页

作者： hongjun Yu Jun Fei Xue Luo Zhongxiang Yao Department of Rehabilitation Southwest Hospital Third Military Medical University Chongqing 400038 China Department of Histology and Embryology Third Military Medical University Chongqing 400038 China Trauma Center of Daping Hospital Third Military Medical University Chongqing 400038 China

BACKGROUND： Studies have demonstrated that ng2-positive glial cells in the adult rats are predominantly located in the gray and white matter of the cerebral cortex and hippocampus. Platelet-derived growth factor-a receptor （PDGF-αR） cells are a subset of oligodendrocytes, which are not as mature as ng2-positive cells. Distribution and migration of PDGF-αR-positive cells in the rat brain remain poorly understood. OBJECTIVE： Using immunohistochemical methods, the distribution of oligodendrocyte precursor cells （PDGF-αR-positive） was analyzed in the adult rat brain. DESIGN, TIME AND SETTIng： Immunohistochemical study was performed at the Department of Histology and Embryology of the Third Military Medical University from September 2007 to September 2008. MATERIALS： Rabbit anti-PDGF-αR polyclonal antibody was purchased from Santa Cruz Biotechnology, USA. Streptomycin-avidin-biotin complex immunohistochemistry kit was purchased from Zhongshan Goldenbridge Biotechnology, china. METHODS： Whole brains from 5 healthy, adult, Wistar rats were collected for immunohistochemistry, and the mean value of PDGF-αR-expressing cells was quantified. The absolute values were translated to ranked data of high, moderate, and low grades （high grade： 10 positive cells; moderate grade： 5-9 cells; low grade：〈 5 cells in a 400 × visual field）. Based on the number of cell processes and branches, as well as the number of PDGF-αR-positive cells, in different regions, the cells were classified into three categories, i.e., type Ⅰ-Ⅲ. From type I to type Ill, the number of processes gradually increased. MAIN OUTCOME MEARSURES： The number and distribution of PDGF-αR-positive cells in different brain regions of adult rats. RESULTS： PDGF-αR-positive cells were located in the forebrain and midbrain, but not in the cerebellum or brainstem. In the olfactory bulb and hippocampus, a total of 60% PDGF-αR-positive cells were type Ⅰ and these cells were not mature as others. In the cerebral cortex, olfactory s

关键词： Platelet-derived growth factor-α receptor oligodendrocyte progenitor cells brain distribution

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Methane in soil gas and its transfer to the atmosphere in the Yakela condensed gas field in the Tarim Basin,Northwest china

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Petroleum Science 2013年第2期10卷 183-189页

作者： Tang Junhong Wang Guojian Yin Haoyong Li hongjun College of Materials & Environmental Engineering Hangzhou Dianzi University Wuxi Research Institute of Petroleum Geology Research Institute of Petroleum Exploration and ProductionSINOPEC

In this study, by analyzing ch4 concentration and 613Cch4 in soil-gas profiles, the potentials of ch4 gas transfer from ground to atmosphere were studied at four representative sectors in the Yakela condensed gas field in the Tarim Basin, Xinjiang, china. These are： 1） the oil-gas interface sector, 2） fault sector, 3） oil-water interface sector, 4） an external area. Variation in ch4 in soil-gas profiles showed that ch4 microseepage resulted from the migration of subsurface hydrocarbon from deep-buried reservoirs to the earth＇s surface. It was found that ch4 from deep-buried reservoirs could migrate upwards to the surface through faults, fissures and permeable rocks, during which some ch4 was oxidized and the unoxidized methane remained in the soil or was emitted into the atmosphere. The lowest level of ch4 at the soil-gas profile was found at the ch4 gas-phase equilibrium point at which the ch4 migration upwards from deep-buried reservoirs and the ch4 diffusion downwards from the atmosphere met. The 613Cch4 and ethane, propane in soil gas exhibited thermogenic characteristics, suggesting the occurrence of ch4 microseepage from deep-buried reservoirs. A linear correlation analysis between ch4 concentrations in soil gas and temperature, moisture, pH, Eh, Ec and particle size of soil indicated that both soil Eh and soil temperature could affect ch4 concentration in soil gas while soil pH could indirectly influence soil methanotrophic oxidation via impacting soil Eh.

关键词： Soil gas ch4 concentration carbon isotope microseepage oil reservoir

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Arrhythmogenic properties of dismantling cadherin-mediated adhesion in murine hearts

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The Journal of Biomedical Research 2010年第4期24卷 292-300页

作者： hongjun Zhu Hegui Wang Xiwen Zhang Xiaofeng Hou Kejiang Cao Jiangang Zou Department of Cardiology the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 Jiangsu Province China

Objective： To evaluate the arrhythmogenic effects of dismantling cadherin-mediated adhesion by recombinant mouse aminopeptidase N （rmAPN） in murine hearts. Methods： rmAPN was incubated with cultured neonatal rat cardiomyocytes as well as being infused in adult mice. The cell-cell connections were immunolabelled and observed by laser confocal microscopy. Disruption of the N-terminal of N-cadherin （N-cad） was detected by western blot and quantitative immunofluorescence. The risk of inducible ventricular tachyarrhythmia was evaluated in mice by an electrophysiological study. Results： Disrupted cell-cell contact was observed in cultured neonatal rat cardiomyocytes in response to 30-40 ng/μL rmAPN. Loss of the N-terminal in N-cad and altered distribution of connexin 43 （Cx43） were observed in hearts from rmAPN-infused mice. In addition, a reduction of phosphorylated Cx43 was also detected concomitant with redistribution of Cx43. Electrophysiological studies of rmAPN-infused mice showed prolonged QRS duration and increased inducibility of ventricular tachycardias. Conclusion： Disruption of N-cad by rmAPN contributes to gap junction remodeling and may elicit arrhythmogenic effects. The disorder of adherent junctions by proteolytic enzymes may play an important role in arrhythmogenic mechanisms in correlated diseases.

关键词： adherence junction N-cadherin gap junction ventricular tachyarrhythmia recombinant mouse aminopeptidase N

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桂枝加龙骨牡蛎汤结合针刺治疗恶性肿瘤相关性失眠的效果观察

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中华中医药学刊 2024年第7期42卷 210-213页

作者：王宏君孙亚男王晓燕张润莲王朝晖倪广林陈玺龙郑佳仪河北北方学院附属第一医院河北张家口075000 河北北方学院针推系河北张家口075000

目的探究桂枝加龙骨牡蛎汤结合针刺治疗恶性肿瘤相关性失眠(Cancer related insomnia, CRI)的效果。方法选择2022年10月—2023年1月来医院就诊的CRI患者100例,按照随机数表法,分成观察组(50例)和对照组(50例)。对照组采用常规安眠药治疗... 详细信息

目的探究桂枝加龙骨牡蛎汤结合针刺治疗恶性肿瘤相关性失眠(Cancer related insomnia, CRI)的效果。方法选择2022年10月—2023年1月来医院就诊的CRI患者100例,按照随机数表法,分成观察组(50例)和对照组(50例)。对照组采用常规安眠药治疗,观察组采用桂枝加龙骨牡蛎汤结合针刺治疗。比较两组的临床疗效、不良反应发生情况,比较两组治疗前、后的中医证候积分、匹兹堡睡眠质量指数(Pittsburgh sleep quality index, PSQI)、神经递质[5-羟吲哚乙酸(5-hydroxyindole acetic acid, 5-HIAA)、5-羟色胺(5-hydroxytryptamine, 5-HT)]含量及炎性因子[白细胞介素-4(Interleukin-4,IL-4)、白细胞介素-6(Interleukin-6,IL-6)及白细胞介素-1β(Interleukin-1β,IL-1β)]变化。结果治疗后,观察组的总有效率为94.00%(47/50),高于对照组(86.00%,43/50)(Png/mL、(22.63±6.05)ng/mL、(16.78±3.22)ng/mL,均低于对照组[(20.74±3.06)ng/mL、(28.94±5.17)ng/mL、(19.43±4.59)ng/mL](Png/mL、(14.54±3.16)ng/mL,均高于对照组[(0.53±0.08)ng/mL、(11.72±2.28)ng/mL](P<0.05);治疗期间,两组均未发生特殊不良反应。结论桂枝加龙骨牡蛎汤结合针刺治疗CRI具有良好效果,可提高患者睡眠质量,调节神经递质及炎性因子水平,具有一定安全性。

关键词：肿瘤相关性失眠桂枝加龙骨牡蛎汤针刺疗效

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Performance of highly flexible sub-cable for REBCO Cable-In-Conduit conductor at 5.8 T applied field

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Superconductivity 2022年第3期3卷 127-132页

作者： Guanyu Xiao Huan Jin chao Zhou hongjun Ma Donghu Wang Fang Liu Huajun Liu Arend Nijhuis Arnaud Devred Institute of Plasma Physics Chinese Academy of SciencesPO Box 1126HefeiAnhui 230031China University of Science and Technology of china No.96JinZhai Road Baohe DistrictHefeiAnhui 230026China University of Twente Faculty of Science and Technology7522 NB EnschedeThe Netherlands European Organization for Nuclear Research(CERN) CH-1211 GenevaSwitzerland

Due to the high current capability and excellent flexibility,High Flexible REBCO Cables(HFRC)have emerged as an important candidate for composite high‐temperature superconducting *** REBCO six around one Cable‐In‐Conduit Conductor(CICC)concept has been designed for application in the Central Solenoid(CS)coil of the china Fusion Engineering Test *** the application of fusion devices,the performance of CICC under electromagnetic(EM)loading and thermal stress is very important for reliable and economic ***,a 1.22 m long sub‐cable with HFRC design for CICC was manufactured and tested at 4.2 K in a background magnetic field up to 5.8 *** aim is to investigate the stability of the current‐carrying properties of the HFRC cable under electromagnetic and thermal cyclic *** test results show that the critical current(I_(c))of the HFRC cable reached 17.3 kA in a background magnetic field of 5.8 T at 4.2 ***,no performance degradation was observed after 24 cycles of 80 kN/m peak load with a background field of 5.8 T and 8 warm‐up‐cool‐down cycles between 77 K and room *** test results provide a good basis for the development of full‐size conductors in future magnet applications.

关键词： REBCO HFRC cable CICC Electromagnetic cycling Performance degradation

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