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Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics

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Chinese Chemical Letters 2022年第5期33卷 2701-2704页

作者： Shihui Qiu Chuanjie Shen Xiaoyu jian Yunxing Lu Zhaoduo Tong Zhenhua Wu hongju Mao jianlong Zhao State Key State Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Tumor heterogeneity plays a critical role in the determination of appropriate anticancer *** cir-culating tumor cells(CTCs)contain all tumor-related information,the genetic changes on CTCs could help us choose the appropriate treatments for different ***-base mutations are very common in tumor genetic changes which may result in drug ***,we introduce a single-cell mutation de-tection platform based on droplet *** platform integrates cell capsulation,cell lysis,poly-merase chain reaction(PCR)and the observation *** droplets’generation speed is over 6000 per minute and more than 600 cells could be encapsulated in one *** verify the performance of our platform in practical use,we performed the mutation analysis of 4 kinds of cells with our platform and noted that the genetic status of each single cell was clearly ***,these results agreed with those from direct *** with other forms of single-cell mutation detection techniques,our platform has high throughput,short experimental time and less experimental operations.

关键词： Single cell CTCs Point mutation EGFR Microfluidics

Multiple exosome RNA analysis methods for lung cancer diagnosis through integrated on-chip microfluidic system

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Chinese Chemical Letters 2022年第6期33卷 3188-3192页

作者： Yunxing Lu Zhaoduo Tong Zhenhua Wu Xiaoyu jian Lin Zhou Shihui Qiu Chuanjie Shen Hao Yin hongju Mao State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Exosomes are now raising focus as a prospective biomarker for cancer diagnostics and prognosis owing to its unique bio-origin and *** take part in cellular communication and receptor mediation and transfer their cargos(e.g.,proteins,m RNA and DNA).Quantitative analysis of tumor-related nucleic acid mutations can be a potential method to cancer diagnosis and prognosis in early *** we present an integrated microfluidic system for exosome on-chip isolation and lung cancer RNA analysis through droplet digital PCR(dd PCR).Gradient dilution experiments show great linearity over a large concentration range with R^(2)=*** the system,four cell lines and two mutation targets were parallelly detected for mutation *** experiments demonstrated mutation heterogeneity and the results were agree with cell *** results proved our integrated microfluidic system as a promising means for early cancer diagnosis and prognosis in the era of liquid biopsy.

关键词： Exosome Integrated processing RNA analysis Microfluidics Droplet digital PCR

A 3D bio-printed spheroids based perfusion in vitro liver on chip for drug toxicity assays

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Chinese Chemical Letters 2022年第6期33卷 3167-3171页

作者： Tian Tian Yuhan Ho Chen Chen Hebin Sun Janan Hui Panhui Yang Yuqing Ge Ting Liu jian Yang hongju Mao Institute of Microsystem and Information Technology(SIMIT) Chinese Academy of Sciences(CAS)Shanghai 200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Shanghai Industrial Technology Research Institute(SITRI) Shanghai 201800China School of Stomatology Dalian Medical UniversityDalian 116044China State Key Laboratory of component-based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China Institute of Traditional Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China

Liver is the foremost organ of human being for drug metabolism,and it played a significant role in toxicity evaluation of *** a liver model in vitro can accelerate the process of the drug screening and new drug research and *** provide a 3D printing based hepatic sinusoid-on-a-chip microdevice that reconstitutes organ-level liver functions to create a drug screening model of toxicity evaluation on *** microfluidic device,which recapitulates the hepatic sinusoid microenvironment,consists of PET polyporous membranes which mimic the perisinusoidal space,and experience fluid flow to mimic the hepatic arterial *** PET membrane was used to separate the hepatocyte and *** endotheliocyte was cultured on the downside of the membrane and the hepatocyte were 3D seeded on the membrane via the 3D *** device was used to reproduce the in vitro liver model for drug toxicity *** expression of several biomarkers of liver was compared with the monoculture and 2D cultured conditions,and the results reveal that this organ-on-a-chip microdevice mimics the drug hepatoxicity that has not been possible by 2D cell-based and animal models,providing a useful platform for screening the drugs and developing an effective therapy in hepatopathy.

关键词： Liver-on-chip Drug screening Drug toxicity 3D-printing 3D-culture

Rapid Synthesis and Photoluminescence Properties of Eu-doped ZnO Nanoneedles via Facile Hydrothermal Method

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Chemical Research in Chinese Universities 2014年第4期30卷 538-542页

作者： LANG Jihui WANG Jiaying ZHANG Qi XU Songsong HAN Qiang ZHANG Yan ZHAI hongju CAO jian YAN Yongsheng YANG Jinghai Scholl of Chemistry and Chemical Engineering Jiangsu University Zhenjiang 212013 P. R. China Institute of Condensed State Physics Key Laboratory of Functional Materials Physics and Chemistry Ministry of Education Jilin Normal University Siping 136000 P. R. China

Eu-doped ZnO nanoneedles with different doping concentrations were prepared via the facile hydrothermal *** crystal structure,morphology and photoluminescence property of the ZnO nanoneedles were characterized by X-ray diffraction（XRD）,scanning electron microscopy（SEM）,X-ray photoelectron spectroscopy（XPS）,photoluminescence spectroscopy（PL） and Raman *** results show that the europium ions are incorporated into the crystal lattice of ZnO matrix in trivalent *** nanoneedles are 2-3 μm in length and 100 nm in the tip *** and Raman measurements indicate that higher Eu^3＋ doping concentration may destroy the crystallization of the nanoneedles and decrease the ratio of IUV/IDLE,which is mainly due to the more defects in the doped ZnO *** the characteristic red emissions of Eu^3＋ ions are found by the PL spectroscopy with the Eu^3＋doping concentration increasing,which are attributed to the ^5D0→^7F0,^5D0→^7F1 and ^5D0→^7F2 transitions.

关键词： ZnO nanoneedle Hydrothermal method Europium ion Crystal structure Photoluminescence property

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利用齐次距离构造最优码（英文）

中国科学技术大学学报 2015年第7期45卷 588-593页

作者：丁健李红菊安徽新华学院公课部

利用R(pm,k)=Fpm[u]/上任意长度的(1+λu)常循环码的挠码得到了R(pm,k)上任意长度的(1+λu)常循环码的齐次距离的界,并确定了R(pm,k)上某些(1+λu)常循环码的齐次距离的准确值,其中λ是R(pm,k)上的单位.此外,定义了从RN(pm,k)(Homogene... 详细信息

利用R(pm,k)=Fpm[u]/上任意长度的(1+λu)常循环码的挠码得到了R(pm,k)上任意长度的(1+λu)常循环码的齐次距离的界,并确定了R(pm,k)上某些(1+λu)常循环码的齐次距离的准确值,其中λ是R(pm,k)上的单位.此外,定义了从RN(pm,k)(Homogeneous距离)到Fpm(k-1)Npm(Hamming距离)的一个新的保距Gray映射,得到R(pm,k)上任意长度的线性(1+λu)常循环码的Gray像是Fpm上的线性码,构造了F2、F3和F4上的一些最优线性码.

关键词：最优码常循环码 Homogeneous距离 Gray映射

环F_2+uF_2+u^2F_2上的(1+u)常循环码

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中国科学技术大学学报 2015年第1期45卷 40-47页

作者：丁健李红菊安徽新华学院公课部安徽合肥230088

基于(xn-1)在F2[x]上的分解,研究了环R=F2+uF2+u2 F2上任意长度的(1+u)常循环码的秩和极小生成元集,定义了环R到F42的一个新的Gray映射,确定了环R上任意长度的(1+u)常循环码的Gray象的结构及Gray象的生成多项式,得到了一些最优的二元线... 详细信息

基于(xn-1)在F2[x]上的分解,研究了环R=F2+uF2+u2 F2上任意长度的(1+u)常循环码的秩和极小生成元集,定义了环R到F42的一个新的Gray映射,确定了环R上任意长度的(1+u)常循环码的Gray象的结构及Gray象的生成多项式,得到了一些最优的二元线性循环码.

关键词：常循环码秩极小生成元集 Gray映射

环F_(p^m)[u]/上一类常循环码的Gray像（英文）

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中国科学技术大学学报 2017年第7期47卷 610-620页

作者：丁健李红菊安徽新华学院通识教育部安徽合肥230088

令R_((p^m,k))=F_(p^m)[u]/,其中p^(j-1)+1≤k≤p^j、u^k=0、p为正素数、j为正整数.定义了从R_((p^m,k))到F_(p^m)^(p^j)的一个新的Gray映射,得到了环R_((p^m,k))上码长为任意长度N的线性(1+u+…+u^(k-1))常循环码的Gray像是F_(p^m)上长... 详细信息

关键词：线性码循环码常循环码 Gray映射最优码

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基于农田景观安全格局的土地整理项目规划

农业工程学报 2009年第5期25卷 217-222页

作者：李红举林坚阎红梅北京大学城市与环境学院北京100871 南水北调建设委员会办公室设计管理中心北京100053

在土地整理活动中,如何在保证增加耕地数量、提高耕地质量的前提下,通过有效的技术手段,因地制宜地建立农田景观安全格局,是土地整理亟待探讨的命题。该文以北京市密云县高岭镇土地整理项目为例,应用以保障水体安全为核心,分析确定了样... 详细信息

在土地整理活动中,如何在保证增加耕地数量、提高耕地质量的前提下,通过有效的技术手段,因地制宜地建立农田景观安全格局,是土地整理亟待探讨的命题。该文以北京市密云县高岭镇土地整理项目为例,应用以保障水体安全为核心,分析确定了样本区的土地整理主导方向和分区,在此基础上提出生物护坎、污染源隔离、河道生态防护等一系列工程技术并予以试验。结果表明,构建以水体安全为核心的生态基础设施,是实现农田景观安全格局的主要途径。

关键词：土地整理项目规划农田景观安全格局北京市

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一种构造GC常重量DNA码的方法

电子与信息学报 2019年第10期41卷 2423-2427页

作者：梁静李红菊赵凤丁健安徽新华学院通识教育部合肥230088 福建师范大学数学与信息学院福州350117

GC重量是DNA码的一个重要参数,如何构造满足GC常重量约束的DNA码是一个有趣的问题。该文通过在DNA码与四元码之间建立一个双射,将构造满足GC常重量约束的DNA码转化为构造GC常重量四元码。通过代数的方法,构造了3类满足GC常重量约束的DN... 详细信息

GC重量是DNA码的一个重要参数,如何构造满足GC常重量约束的DNA码是一个有趣的问题。该文通过在DNA码与四元码之间建立一个双射,将构造满足GC常重量约束的DNA码转化为构造GC常重量四元码。通过代数的方法,构造了3类满足GC常重量约束的DNA码。

关键词：四元码 DNA码 GC重量