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Journal of Integrative Agriculture 2024年第9期23卷 3238-3242页

作者： Xueliang Zhao Yongqiang Miao Hongmei Chen honghu shan Juan Wang Yang Wang Jianzhong Shen Zengqi Yang National Key Laboratory of Veterinary Public Health Security College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China College of Veterinary Medicine Northwest A&F UniversityYangling 712100China College of Food Science and Engineering Northwest A&F UniversityYangling 712100China

Moellerella wisconsensis is proposed for a member of the family Enterobacteriaceae previously designated as Enteric Group *** 1984,the majority of the bacterial isolates(6/8,75%)had been identified in Wisconsin,USA(Hickman-Brenner et al.2021).Thus,this bacterial was named *** the passage of nearly four decades since its discovery,limited research has been conducted on this *** may be attributed to the misidentification of *** as Escherichia coli(Stock et al.2003).Currently,the presence of these microorganisms has been identified in both humans and animals,leading to the development of various clinical symptoms such as diarrhea,urinary tract infections,and bacteremia(Aller et al.2009;Leroy et al.2016).

关键词： Brenner passage

Establishment of a humanized mouse model using steady-state peripheral blood-derived hematopoietic stem and progenitor cells facilitates screening of cancer-targeted T-cell repertoires

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Cancer Innovation 2024年第3期3卷 1-21页

作者： Yulin Xu Wei shan Qian Luo Meng Zhang Dawei Huo Yijin Chen honghu Li Yishan Ye Xiaohong Yu Yi Luo He Huang Bone Marrow Transplantation Center The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina Liangzhu Laboratory Zhejiang UniversityHangzhouChina Institute of Hematology Zhejiang UniversityHangzhouChina Zhejiang Province Engineering Research Center for Stem Cell and Immunity Therapy HangzhouChina School of Medicine Zhejiang UniversityHangzhouChina

Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast ***,approaches to obtain cancer-reactive TCR-T cells have been ***:Here,we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune *** steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized *** human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and *** fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 ***,human T cells were processed for TCRβ-chain(TRB)sequencing *** the repertoires had been constructed,features such as the fraction,diversity,and immune signature were ***:The results demonstrated an increase in diversity and clonality of T cells after *** preferential usage and features of TRBV,TRBJ,and the V–J combination were also *** stress also induced highly clonal Science and Technology,Grant/Award Number:2021C03010;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004 *** burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized ***:We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T *** platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells.

关键词： cancer-targeted T-cell receptor T(TCR-T)cells circulating hematopoietic stem and progenitor cells(HSPCs) humanized mouse model steady-state peripheral blood T-cell receptorβ-chain(TRB) three-dimensional culture

Effects of typical karst tourist town on the Lijiang River water: from heavy metals to N-nitrosamines

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Journal of Earth Science 2024年

作者： Yuanyu shan Yuru Su Xinli Xing Bingquan Zhu honghu Zeng Yuan Zhang Junyu Li Xiaoyu Yan Wenwen Chen Benjian Li College of Environmental Science and Engineering， Guilin University of Technology School of Environmental Studies， China University of Geosciences State Key Laboratory of Biogeology and Environmental Geology， China University of Geosciences College of arts， Guilin University of Technology College of arts and communication， China University of Geosciences 430070 China

N-nitrosamines are nitrogenous organic pollutants with high carcinogenicity to human. Heavy metal pollution in aquatic system is currently a major environmental problem. Tourism in typical karst areas may influence the environment through wastewater discharge. However， it remains unclear whether these activities would cause water pollution. To evaluate the impact of tourism activities in typical tourist towns， this study investigated the occurrence and sources of 18 N-nitrosamines， eight heavy metals （HMs）， and dissolved inorganic nitrogen （DIN） in the Lijiang River and its tributaries. Seven N-nitrosamines were detected as the dominant compounds in N-nitroso-N-ethylaniline （NEA， average of 24.8 ng/L） and N-nitroso-l-proline （NPRO， 20.4 ng/L）， while especially low concentrations of HMs （Mn， Cu， As， Ni， Cr， Co， Pb， and Cd） and DIN (NH4+-N， NO2--N， and NO3--N) were observed. The most important influencing factors were seasonal factors， followed by land-use type and mainstem and tributary factors. Principal component analysis revealed that in the city with developed tourism and relatively less heavy industry， aquaculture and agricultural wastewater were identified as the primary sources of N-nitrosamines. Meanwhile， domestic sewage and agricultural wastewater were found to be the main sources of HMs and DIN， respectively. The ecological risk assessments for all pollutants are relatively low （< 1）. Therefore， N-nitrosamines， HMs， and DIN pollution caused by tourist activities pose a negligible risk to the Lijiang River.

关键词：

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一例羔羊链球菌性肺炎病例的实验室诊断

黑龙江畜牧兽医 2023年第6期 93-96,143页

作者：党如意白新栋郭晓凡单鸿鹄吴克杨增岐西北农林科技大学动物医学院陕西杨凌712100

2022年3—4月份,陕西关中某奶山羊养殖场暴发呼吸系统疾病,导致约150只奶山羊发病死亡。为了明确羔羊发病原因,试验对5只病死羔羊进行了剖检,并采集其肺脏、胸腔积液和心包积液进行细菌分离鉴定、耐药性检测及致病性试验。结果表明:死... 详细信息

2022年3—4月份,陕西关中某奶山羊养殖场暴发呼吸系统疾病,导致约150只奶山羊发病死亡。为了明确羔羊发病原因,试验对5只病死羔羊进行了剖检,并采集其肺脏、胸腔积液和心包积液进行细菌分离鉴定、耐药性检测及致病性试验。结果表明:死亡羔羊病变主要在肺部,剖检可见肺脏水肿,心叶、尖叶、膈叶有典型的红色肝变区,切面有化脓灶,且肺脏与胸壁粘连。5只羔羊肺脏中均分离到羊链球菌,大肠杆菌和海藻糖比伯斯坦菌分别在2只羔羊肺脏中分离到,结合肺脏病变初步判断此次羔羊肺炎由羊链球菌引起,或伴发有其他细菌感染。羊链球菌分离株对替米考星、恩诺沙星、链霉素等抗生素均耐药,对阿莫西林、苯唑西林、复方阿莫西林等抗生素敏感。将分离的羊链球菌菌株给小鼠腹腔注射后,小鼠2~6 h内主要表现被毛凌乱、扎堆,48 h内未出现死亡,但剖检发现攻菌小鼠肺脏均出现不同程度出血。说明该养殖场关中奶山羊羔羊主要感染了羊链球菌,同时合并感染了大肠杆菌和海藻糖比伯斯坦菌。

关键词：关中奶山羊肺炎链球菌实验室诊断药敏试验致病性试验

Efficient expansion of rare human circulating hematopoietic stem/progenitor cells in steady-state blood using a polypeptide-forming 3D culture

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Protein & Cell 2022年第11期13卷 808-824页

作者： Yulin Xu Xiangjun Zeng Mingming Zhang Binsheng Wang Xin Guo Wei shan Shuyang Cai Qian Luo honghu Li Xia Li Xue Li Hao Zhang Limengmeng Wang Yu Lin Lizhen Liu Yanwei Li Meng Zhang Xiaohong Yu Pengxu Qian He Huang Bone Marrow Transplantation Center First Affiliated HospitalZhejiang University School of MedicineHangzhou 310012China Liangzhu Laboratory Zhejiang University Medical CenterHangzhou 311121China Institute of Hematology Zhejiang UniversityHangzhou 310012China Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy Hangzhou 310012China Core Facilities Zhejiang University School of MedicineHangzhou 310012China Dr.Li Dak Sum&Yip Yio Chin Center for Stem Cell and Regenerative Medicine Zhejiang UniversityHangzhou 310012China

Although widely applied in treating hematopoietic malignancies,transplantation of hematopoietic stem/progenitor cells(HSPCs)is impeded by HSPC *** circulating HSPCs(cHSPCs)in steady-state blood could be used as an alternative source remains largely *** we develop a three-dimensional culture system(3DCS)including arginine,glycine,aspartate,and a series of ***-day culture of peripheral blood mononuclear cells(PBMNCs)in 3DCS led to 125-and 70-fold increase of the frequency and number of CD34+***,3DCS-expanded cHSPCs exhibited the similar reconstitution rate com-pared to CD34+HSPCs in bone ***,3DCS fabricated an immunomodulatory niche,secreting cytokines as TNF to support cHSPC survival and ***,3DCS could also promote the expansion of cHSPCs in patients who failed in HSPC *** 3DCS successfully expands rare cHSPCs,providing an alternative source for the HSPC therapy,particularly for the patients/donors who have failed in HSPC mobilization.

关键词： hematopoietic stem/progenitor cell transplantation peripheral blood mononuclear cells expansion mobilization three-dimensional culture selfrenewal and multilineage differentiation

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厚顶煤巷道顶帮整体锚固支护体系研究与应用

煤炭科学技术 2018年第4期46卷 93-99页

作者：单仁亮鲍永生原鸿鹄中国矿业大学(北京)力学与建筑工程学院北京100083 大同煤矿集团有限责任公司马脊梁矿山西大同037027

针对深部应力作用及常规支护条件下厚顶煤矩形断面巷道围岩变形量大的问题,分析主要原因是巷道肩部围岩裂隙发育,顶帮锚固体相互独立,支护结构松散。从厚顶煤巷道围岩失稳破坏机理出发,采用FLAC3D数值计算,对顶帮整体锚固支护体系及传... 详细信息

针对深部应力作用及常规支护条件下厚顶煤矩形断面巷道围岩变形量大的问题,分析主要原因是巷道肩部围岩裂隙发育,顶帮锚固体相互独立,支护结构松散。从厚顶煤巷道围岩失稳破坏机理出发,采用FLAC3D数值计算,对顶帮整体锚固支护体系及传统锚固支护的支护预应力场分布特征及巷道围岩变形破坏规律进行了对比分析。结果表明,顶帮整体锚固支护体系能够改善肩部围岩为挤压式传力机制,抑制肩角处围岩剪切裂隙的产生和发育,增强顶帮围岩在空间上的相互支撑作用,有效提高围岩自承能力,防止厚顶煤巷道发生冒顶、片帮等非线性大变形现象。现场应用表明,采用顶帮整体锚固支护体系后,巷道顶板最大下沉量为50 mm,两帮移近量最大为100 mm,支护效果良好。

关键词：厚顶煤巷道整体锚固支护体系围岩稳定性破坏机理

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裂隙红砂岩冻胀力特性试验研究

煤炭学报 2019年第6期44卷 1742-1752页

作者：单仁亮白瑶孙鹏飞吴永鑫隋顺猛原鸿鹄中国矿业大学(北京)力学与建筑工程学院

裂隙岩体冻胀现象在富水岩层冻结工程中普遍存在,冻胀力易使得岩体裂隙尖端应力集中而产生新的微裂纹,微裂纹扩展贯通导致岩体发生不同程度破坏。为揭示裂隙岩体冻融损伤演化机制,探究以冻胀力驱动下的岩体裂隙扩展规律,利用超薄型电阻... 详细信息

裂隙岩体冻胀现象在富水岩层冻结工程中普遍存在,冻胀力易使得岩体裂隙尖端应力集中而产生新的微裂纹,微裂纹扩展贯通导致岩体发生不同程度破坏。为揭示裂隙岩体冻融损伤演化机制,探究以冻胀力驱动下的岩体裂隙扩展规律,利用超薄型电阻式压力传感器、温度传感器、低温恒温箱组成的冻胀力测试系统对不同冻结速率、不同裂隙尺寸、倾角以及不同边界条件下红砂岩贯通裂隙中的冻胀力进行测试,分析了贯通裂隙中冻胀力演化规律、冻胀劣化机制以及冻胀破坏模式。结果表明:含贯通裂隙试样典型冻胀力演化过程分为初始冻结阶段、迅速上升阶段、卸荷阶段、维持阶段、消散阶段;冻胀作用使得贯通裂隙端部附近出现明显裂纹扩展现象,试样整体呈竖向张拉破坏;贯通裂隙最大冻胀力随隙宽呈单指数函数增长、与温度成线性正相关;冻结过程中,试样边界条件对裂隙冻胀力影响显著,刚性约束边界条件下最大冻胀力达到1.27 MPa,融化过程中,不同边界条件对应不同冻胀力跌落现象;低温条件下贯通裂隙红砂岩冻胀力变化特征与冻胀过程中声发射能量变化规律一致,充分验证了冻胀力对贯通裂隙岩体的损伤劣化作用。研究成果可为裂隙岩体冻胀力理论计算与数值分析、冻融损伤机制、冰-岩耦合特性以及煤矿立井冻结壁设计等提供参考。

关键词：冻结壁红砂岩裂隙产状冻胀力冻胀扩展

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国内外煤巷支护技术研究进展

岩石力学与工程学报 2019年第12期38卷 2377-2403页

作者：单仁亮彭杨皓孔祥松肖禹航原鸿鹄黄博郑赟中国矿业大学(北京)力学与建筑工程学院北京100083 安徽工程大学建筑工程学院安徽芜湖241000 华北科技学院安全工程学院河北三河065201

简要总结我国煤巷支护领域现阶段的部分主要成果,同时阐述国外煤巷支护技术研究现状。国内煤巷支护技术近些年主要是围绕锚杆支护而开发的多种单一或组合支护系统,但是煤巷支护现场不断涌出了的新问题;国外煤巷支护系统具有多样性,为我... 详细信息

简要总结我国煤巷支护领域现阶段的部分主要成果,同时阐述国外煤巷支护技术研究现状。国内煤巷支护技术近些年主要是围绕锚杆支护而开发的多种单一或组合支护系统,但是煤巷支护现场不断涌出了的新问题;国外煤巷支护系统具有多样性,为我国煤巷支护理论、装备及技术研究的进一步完善、多元化发展尤其是千米深井煤巷围岩控制带来了有益的启发。笔者综合采用理论分析、模型试验、数值模拟及现场试验等多种研究方法对煤巷支护深入研究,提出煤巷强帮强角支护理论与技术、纵向梁复合式支护技术、协同支护技术、抗剪锚管索支护技术,实现了真正意义上的"锚杆锚索一体化(协同)支护"。此外,基于研制的动压巷道物理模型试验装置,改进了煤巷支护模拟技术,然后讨论了每项技术的创新点、适用条件及意义、存在的不足及改进方向。最后,基于上述研究成果,提出了我国煤巷支护技术发展趋势与建议,未来煤巷支护将采用多种主动支护工艺相结合或主被动支护相结合等多元化方法,并逐步向智能支护方向发展。

关键词：采矿工程煤巷支护强帮强角协同支护抗剪锚管索动压巷道技术进展智能支护

群体感应抑制剂对费氏弧菌生物荧光随时间变化Hormesis效应的机制探究

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环境化学 2021年第5期40卷 1352-1360页

作者：赵姗曾鸿鹄孙昊宇林志芬莫凌云桂林理工大学环境科学与工程学院桂林541004 污染控制与资源化研究国家重点实验室同济大学环境科学与工程学院上海200092

群体感应抑制剂(quorum sensing inhibitor,QSIs)是作用于细菌群体感应系统的一类新型抗菌药物。目前关于QSIs引起Hormesis效应的机制探究较少。本文以费氏弧菌(Aliivibrio fischeri,***)为模式生物,测定了3种高丝氨酸内酯(N-Acyl homos... 详细信息

群体感应抑制剂(quorum sensing inhibitor,QSIs)是作用于细菌群体感应系统的一类新型抗菌药物。目前关于QSIs引起Hormesis效应的机制探究较少。本文以费氏弧菌(Aliivibrio fischeri,***)为模式生物,测定了3种高丝氨酸内酯(N-Acyl homoserine lactone,AHL)类QSIs(AHL-QSIs)对***生物荧光的影响,发现AHL-QSIs对***生物荧光产生了随时间变化的Hormesis效应。通过测定AHL-QSIs对***生长量、相关蛋白表达量及模拟发光反应的影响,推测随时间变化Hormesis的机制如下:AHL-QSIs在低浓度时可以通过增强荧光素酶的活性进而促进个体发光反应,最终刺激***的生物荧光;而当AHL-QSIs处于高浓度时,更多的AHL-QSIs不仅会通过改变细胞膜功能抑制细菌的生长,还可以与C6竞争结合LuxR影响荧光素酶的含量,最终抑制***的生物荧光;AHL-QSIs对***生物荧光的刺激作用和抑制作用会随细菌生长阶段状态的不同而发生变化,最终导致了随时间变化的Hormesis效应。研究结果可为今后AHL-QSIs的生态风险及其进一步的广泛应用提供一定的数据基础和理论依据。

关键词：群体感应抑制剂 Hormesis 群体感应系统费氏弧菌随时间变化

钢管混凝土支架作用下南关矿煤巷变形破坏规律

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矿业科学学报 2020年第2期5卷 160-168页

作者：孔祥松单仁亮肖禹航鲍甜甜原鸿鹄中国矿业大学(北京)力学与建筑工程学院北京100083

以汾西矿区南关矿煤巷为原型,依托嵌套式相似模拟实验系统建立物理相似模型,研究煤巷在钢管混凝土支架支护下围岩应力、位移分布规律和变形破坏特征。应用FLAC3D 5.0建立钢管混凝土支架巷道模型,分别模拟埋深100~1000 m的情况,分析煤巷... 详细信息

以汾西矿区南关矿煤巷为原型,依托嵌套式相似模拟实验系统建立物理相似模型,研究煤巷在钢管混凝土支架支护下围岩应力、位移分布规律和变形破坏特征。应用FLAC3D 5.0建立钢管混凝土支架巷道模型,分别模拟埋深100~1000 m的情况,分析煤巷变形破坏规律及支架受力特点。研究结果表明:钢管混凝土支架能提高巷道的径向支撑压力,加载初期保持了巷道稳定,但随着荷载加大两帮收敛量大于顶底板移近量,帮部先出现裂隙并向顶底板发展,随后帮部和顶板相继失稳,可见帮部是支护的薄弱环节。在钢管混凝土支架作用下,巷道表面应力呈锯齿状分布,说明支架对围岩存在有效支护范围,支架之间是支护的薄弱部位;支架套管的轴力和弯矩均较大,容易发生破坏,导致支架的承载力丧失。可见,控制煤巷帮部和支架套管的变形破坏,提高其强度,是保证南关矿煤巷钢管混凝土支架支护稳定性的关键。

关键词：钢管混凝土支架煤巷围岩变形帮部模型实验