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Rapid detection of fluoroquinolone residues in aquatic products based on a gold-labeled microwell immunochromatographic assay

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Food Quality and Safety 2022年第4期6卷 597-607页

作者： Jiaxu Xiao Hongfei Yang Liangni Qin Jixiang Liang Long Li Xiaohui Fan Dapeng Peng National Reference Laboratory of Veterinary Drug Residues/Key Laboratory of Ministry of Agriculture for the Detection of Veterinary Drug Residues in Foods Huazhong Agricultural UniversityWuhanChina Wuhan Shangcheng Biotechnology Co. Ltd.WuhanChina Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityShenzhenChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Objectives:Fluoroquinolones(FQs)are widely used in aquaculture,and their residues have caused many problems threatening human health.Here,this study aims to develop a colloidal gold immunochromatographic strip based on gold-labeled microwells to screen the residues of FQs on site.Materials and methods:The Protein A Magarose Beads affinity chromatography method was adopted to purify the ascites against FQs.By using a strategy of heterologous coating antigen,different coating antigens are applied to detect FQs.The gold-labeled microwell immunochromatographic assay was used to improve the sensitivity of the test strip by the advanced reaction of antigen and antibody.Results:The antibodies were verified to be of high purity up to 99%,and the titer reached 1:1024000.The combination(enoxacin-OVA and the antibody)detected the 4 banned FQs(pefloxacin,PEF;norfloxacin,NOR;lomefloxacin,LOM;ofloxacin,OFL)with the 50%inhibiting concentration(IC50)values ranging from 1.3 to 2.1 ng/mL and cross-reactions ranging from 67.3%to 106.1%.The analysis of spiked crucian carp,silver carp,grass carp,and shrimp samples showed that the limit of detection for PEF,NOR,LOM,and OFL was 4μg/kg.A comparative study with liquid chromatography tandem mass spectrometry(LC-MS/MS)demonstrated that the assay provided an effective screening tool for the rapid detection of FQs residues.Conclusions:The results indicated that the test strip can realize full coverage recognition of the 4 banned FQs and has good accuracy,specifi-city,reproducibility,and stability;therefore,they are more suitable for rapid detection of FQs in aquatic products.

关键词： fluoroquinolones colloidal gold microwell purification residues

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Development of 3D top coal caving angle model for fully mechanized extra-thick coal seam mining

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International Journal of Mining Science and Technology 2022年第5期32卷 1145-1152页

作者： hongfei Duan Lijuan Zhao Haiyan yang Yao Zhang Hongchun Xia China University of Mining and Technology Xuzhou 221116China Jinneng Holding Group Taiyuan 030000China College of Coal Engineering Shanxi Datong UniversityDatong 037000China College of Civil and Architectural Engineering Dalian UniversityDalian 116622China

During high-intensity,fully mechanized mining of extra-thick coal seam,the top coal would cave to a certain 3D form.Based on the data collected during drilling,a 3D model of top coal caving surface space was established to determine the relationship between the location of the stope roof and the caving surface,enabling the mathematical computation of the top caving angle(φ).The drilling method was employed to measure the top caving angle on two extra-thick fully mechanized coal caving faces under the conditions of three geological structures,namely,no geological structure,igneous rock structure,and fault structure.The results show that the value of top caving angle could be accurately estimated on-site with the 9-parameter 3D top coal caving surface model built with the drilling method.This method is a novel on-site measurement that can be easily applied.Our findings reveal that the characteristics of the coal-rock in the two mining faces are different;yet their caving angles follow the ruleφ_(igneous rock structure)<φ_(no geological structure)<φ_(fault structure).Finally,through the data fitting with two indexes(the top coal uniaxial compressive strength and the top caving angle),it is found that the relationship between the two indexes satisfies an exponential decay function.

关键词： Space model Field measurement Top coal caving angle Uniaxial compressive strength Exponential decay function

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Role of temperature in tribolayers in fretting wear ofγ-TiAl alloy

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Friction 2024年第5期12卷 939-953页

作者： Yulei yang hongfei SHANG Huiping PEI Jimin XU Yi LIANG Minghui PAN School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China State Key Laboratory of Tribology in Advanced Equipment Tsinghua UniversityBeijing100084China Institute of Tribology Hefei University of TechnologyHefei230009China

The formation of tribolayers may play significant influences on fretting wear.At elevated temperature,the adhesion among wear debris and the increased diffusion rate facilitate the formation of tribolayers.However,the intensification of oxidation at elevated temperature and the low diffusion rate in oxides may play an adverse role.The present study aims to investigate the role of temperature in tribolayers in fretting wear using aγ-TiAl alloy.Scanning electron microscope,energy dispersive spectrometer,Raman spectrometer,transmission electron microscope and nanoindentation were utilized to investigate the wear debris,tribolayers,and wear scars.The fretting tests showed that,compared with that at room temperature(RT)and 350℃,significant reduction in wear rate and decrease in the fluctuation of friction coefficient occurred at 550 and 750℃.It was further revealed that when temperature raised from room temperature(RT)to 750℃,the oxidation of the wear debris increased slightly and the diffusion coefficients increased prominently,which facilities the formation of well tribo-sintered tribolayers.The well tribo-sintered tribolayers presented homogenous structure,nanocrystalline grains with excellent mechanical properties,and resulted in the improvement in the fretting wear resistance of theγ-TiAl alloy at 550 and 750℃.

关键词： tribolayer temperature fretting wear γ-TiAl alloy

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Overview of Planar Magnetics for High-frequency Resonant Converters

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Chinese Journal of Electrical Engineering 2022年第4期8卷 61-78页

作者： Yue Liu Yufeng Song Dingfan Hu yang Li Zuoqian Zhang hongfei Wu Department of Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing 211106China

With the continuous development of power supplies toward miniaturization,light weights,and high levels of integration,research on high-frequency resonant conversion based on planar magnetics is becoming extensive.Combining the soft-switching characteristics of resonant converters with those of wide bandgap devices,the switching frequency can be increase to the MHz range,and the power density of the entire system can be improved considerably.However,higher switching frequencies impose new requirements for the structural design,loss distribution,and common mode(CM)noise suppression of passive magnetic components.Herein,a thorough survey of the-state-of-the-art of planar magnetics in high-frequency resonant converters is conducted.Printed circuit board winding-based planar magnetics,magnetic integration,and power-loss optimization strategies are summarized in detail.Suppression methods for CM noise in high-frequency planar magnetics are also clarified and discussed.An insight view into the future development of planar magnetics for high-frequency resonant converters is presented.

关键词： Planar magnetics resonant converter matrix transformer magnetic integration PCB winding loss model loss measurement CM noise suppression

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Solvent-Free Synthesis of Self-Healable and Recyclable Crosslinked Polyurethane Based on Dynamic Oxime-Urethane Bonds

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Chinese Journal of Polymer Science 2023年第11期41卷 1725-1732,I0007页

作者： Yuepeng Wang Lei yang Luzhi Zhang hongfei Huang Bo Qian Shijia Gu Zhengwei You State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Institute of Functional MaterialsCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620China

Polyurethane is widely used for its versatility in design and range of performance.Self-healing and recyclable dynamic polyurethane networks have attracted extensive attention due to their potential to extend service life and ensure safety in use,as well as to promote sustainable use of resources.Developing green and environment-friendly methods to obtain this material is an interesting and challenging task,as the majority of current dynamic polyurethane networks utilize the solution polymerization method.The use of solvents makes the processes complicated,harmful to environment,and increase the cost.Poly(oxime-urethanes)(POUs)are emerging dynamic polyurethanes and show great potential in diverse fields,such as biomaterials,hot melt adhesives,and flexible electronics.In this study,we utilized the solubility properties of dimethylglyoxime in raw material poly(ethylene glycol)to prepare POUs through bulk polymerization for the first time.This method is simple,convenient and cost-efficient.Simultaneously,copper ion coordination improves POUs strength and dynamic properties,with mechanical strength up from 0.54 MPa to 1.03 MPa and self-healing recovery rate up from 85.5%to 91.8%,and activation energy down from 119.6 k J/mol to 95.4 k J/mol.To demonstrate the application of this technology,self-healing and stretchable circuits are constructed from this dynamic polyurethane network.

关键词： Polyurethane Dynamic covalent bonds Oxime-urethane bonds Self-healing Recycle

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Caterpillar-like 3D graphene nanoscrolls@CNTs hybrids decorated with Co-doped MoSe_(2)nanosheets for electrocatalytic hydrogen evolution

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Journal of Materials Science & Technology 2023年第5期136卷 43-53页

作者： Hao Luo hongfei Gao Xudong Zhang Fan yang Chen Liu Kewei Xu Dagang Guo State Key Laboratory for Mechanical Behavior of Materials School of Materials Science and EngineeringXi’an Jiaotong UniversityXi’an 710049China Center for High Performance Computing Network Information CenterXi’an Jiaotong UniversityXi’an 710049China

Hydrogen is a clean and flexible energy carrier that has the promising to satisfy urgent demands of the energy crisis and environmental protection.Electrochemical hydrogen evolution reaction(HER),a critical half-reaction in water splitting,is one of the greenest and most common methods to obtain high-purity hydrogen.Designing preeminent activity and stability electrocatalysts for hydrogen precipitation reac-tion(HER)to reduce energy consumption is of great essential.3D carbon-based materials have attracted widespread concern as the potential scaffolds of highly active and durable electrocatalysts for HER.To boost the HER activity and prolong the lifespan of electrocatalysts,multifarious 3D carbon architectures make an appearance to be engineered for accelerating electronic/mass transfer and maximizing the expo-sure of active sites.Herein,we designed and fabricated high-performance electrocatalysts based on a spe-cial caterpillar-like 3D graphene nanoscrolls@CNTs(GNS@CNTs)scaffold decorated with Co-doped MoSe_(2)nanosheets for HER.In the caterpillar-like hierarchical structure,CNTs were seamlessly co-bonded and dilated the interlayer and outer spacing of GNS through CVD growth technology,and nickel nanoparticles were covered by the CNTs tips.Taking advantage of the plentiful hierarchical pore,larger specific surface area,and higher chemical stability of the caterpillar-like structure,the catalysts exhibited enhanced elec-trocatalytic properties than some existing data reported.Density functional theory calculations showed that the encapsulated nickel nanoparticle could tune the electronic structure of the outer anchored Co-doped MoSe_(2)and optimize itsG of H∗adsorption by electron traversing effect and doping effect.These indicate that caterpillar-like GNS@CNT is an ideal scaffold f or anchoring actives substance and is suit-able for high-efficient HER.This study provides new insights for designing hierarchical carbon composite nanostructures for catalysts,sensors,energy materials,and other applications.

关键词： Electron traversing effect 3D graphene Graphene nanoscrolls CNT MoSe_(2),Hydrogen evolution reaction

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Development and Field Application of Phosphogypsum-Based Soil Subgrade Stabilizers

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Journal of Renewable Materials 2022年第8期10卷 2247-2261页

作者： hongfei Yue Aiguo Fang Sudong Hua Zenghuan Gu Yu Jia Cheng yang College of Materials Science and Engineering Nanjing Tech UniversityNanjing211800China China Construction Installaction Engineering Co. Ltd.Nanjing210023China Jiangsu Subote New Material Co. Ltd.Nanjing211103China Sinoma Construction Co. Ltd.Suzhou215300China

A phosphogypsum-based subgrade stabilizer(PBSS)was formulated using industrial by-product phosphogypsum(PG),mixed with slag and calcium-silicon-rich active material(GSR).The active powder(AP)was used to modify PBSS,and PBSS-AP was obtained.PBSS and PBSS-AP were each mixed with 10%silty soil,and cement and lime(CAL:5%lime+2%cement)were used as the traditional material for comparative experiments.Samples were cured under standard conditions,and tested for unconfined compressive strength(UCS),water stability,volume expansion,and leachate,to explore the stabilization effect of the three solidified materials on silty soil.The results showed that the comprehensive performance of sility soil mixed with 12%PBSS-AP was the best.The CaO,SiO_(2)and Al_(2)O_(3)provided by PG,Slag and GSR will react with water to form a stable C-S-H gel,which is conducive to stabilizing the soil.Field application results showed that the compaction exceeded 95%,the deflection was 144.9 mm,and UCS was 2.5 MPa after 28 days.These findings indicated that PBSS-AP is an effective stabilizer for subgrade soils.

关键词： Phosphogypsum solidified material unconfined compressive strength water stability volume expansion leachate

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Heat Management System for Long-term Continuous Observation of Electronic Instrumentation in Deep Earth

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Acta Geologica Sinica(English Edition) 2021年第S01期95卷 88-90页

作者： WANG Yanzhang yang hongfei ZHANG Jie SHI Jiaqing National Geophysical Exploration Equipment Engineering Research Center(Jilin University) Changchun 130026China Key Laboratory of Geophysical Exploration Equipment Ministry of Education(Jilin University)Changchun 130026China Key Laboratory of Earth Detection Technology and Equipment Ministry of Land and Resources(Jilin University)Changchun 130026China College of Instrumentation&Electrical Engineering Jilin UniversityChangchun130061China

Deep penetration into the Earth’s interior and direct monitoring of weak changes in physical fields and their cumulative processes and effects in the deep Earth can enhance the identification of deep Earth targets and deepen the degree of knowledge of the details of the deep Earth structure and deep processes(Moskvitch,2014),which is important for promoting the development of Earth system science.

关键词： deep earth exploration scientific logging high-temperature harsh environments heat management recirculation cooling

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Targeting ASIC1a Promotes Neural Progenitor Cell Migration and Neurogenesis in Ischemic Stroke

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Research 2024年第1期2023卷 369-387页

作者： hongfei Ge Tengyuan Zhou Chao Zhang Yupeng Cun Weixiang Chen yang yang Qian Zhang Huanhuan Li Jun Zhong Xuyang Zhang Hua Feng Rong Hu Department of Neurosurgery and Key Laboratory of Neurotrauma Southwest HospitalThird Military Medical University(Army Medical University)400038 ChongqingChina Medical Research Center Southwest HospitalThird Military Medical University(Army Medical University)400038 ChongqingChina Pediatric Research Institute Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChildren’s Hospital of Chongqing Medical University400014 ChongqingChina

Cell replacement therapy using neural progenitor cells(NPCs)has been shown to be an effective treatment for ischemic stroke.However,the therapeutic effect is unsatisfactory due to the imbalanced homeostasis of the local microenvironment after ischemia.Microenvironmental acidosis is a common imbalanced homeostasis in the penumbra and could activate acid-sensing ion channels 1a(ASIC1a),a subunit of proton-gated cation channels following ischemic stroke.However,the role of ASIC1a in NPCs post-ischemia remains elusive.Here,our results indicated that ASIC1a was expressed in NPCs with channel functionality,which could be activated by extracellular acidification.Further evidence revealed that ASIC1a activation inhibited NPC migration and neurogenesis through RhoA signaling-mediated reorganization of filopodia formation,which could be primarily reversed by pharmacological or genetic disruption of ASIC1a.In vivo data showed that the knockout of the ASIC1a gene facilitated NPC migration and neurogenesis in the penumbra to improve behavioral recovery after stroke.

关键词： ASIC1a homeostasis inhibited

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Direct synthesis of tin spheres/nitrogen-doped porous carbon composite by self-formed template method for enhanced lithium storage

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Journal of Materials Science & Technology 2022年第9期104卷 88-97页

作者： Kun Liu Jia-ao Wang hongfei Zheng Xiaodong Sun Zhimo yang Jianzong Man Xinyu Wang Juncai Sun Institute of Materials and Technology Dalian Maritime UniversityDalian 116026China Department of Chemistry and the Oden Institute for Computational Engineering and Sciences The University of Texas at AustinAustinTX78712-0165USA College of Materials Xiamen UniversityXiamen 361005China

To inhibit the agglomeration of tin-based nanomaterials and simplify the complicated synthesis process,a facile and eco-friendly self-formed template method is reported to synthesize tin submicron spheres dispersed in nitrogen-doped porous carbon(Sn/NPC)by pyrolysis of a mixture of disodium stannous citrate and urea.The vital point of this strategy is the formation of Na_(2)CO_(3)templates during pyrolysis.This self-formed Na_(2)CO_(3)acts as porous templates to support the formation of NPC.The obtained NPC provides good electronic conductivity,ample defects,and more active sites.Serving as anode for Li-ion batteries,the Sn/NPC electrode obtains a stable discharge capacity of 674.1 mAh/g after 150 cycles at 0.1 A/g.Especially,a high discharge capacity of 331.2 mAh/g can be achieved after 1100 cycles at 3 A/g.Additionally,a full cell coupled with LiCoO_(2)as cathode yields a discharge capacity of 524.8 mAh/g after 150 cycles at 0.1 A/g.In-situ XRD is implemented to investigate the alloying/dealloying reaction mechanisms.Density functional theory calculation ulteriorly explicates that NPC heightens intrinsic electronic conductivity,and NPC especially pyrrolic-N and pyridinic-N doping facilitates the Li-adsorption ability.Climbing image nudged elastic band method reveals low Li~+diffusion energy barrier in presence of N atoms,which accounts for the terrific electrochemical properties of Sn/NPC electrode.

关键词： Tin submicron spheres Nitrogen-doped porous carbon Self-formed template In-situ XRD Li-ion batteries

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