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Ganoderma Lucidum Spore Oil enhances the effect of cyclophosphamide via inhibiting programmed death-1 and prolongs the survival of H22 tumor-bearing mice

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Journal of Traditional Chinese Medicine 2024年第4期 652-659页

作者： JIANG Zhaojian cai hongfei YUAN Cheng CAO Lin XU Wendong HAN Yaming ZHANG Qin LI Jing WANG Qin LIU Juyan Guangzhou HanFang Pharmaceutical Company Limited National Engineering Research Center of Pharmaceutical Processing Technology of Traditional Chinese Medicine and Drug InnovationGuangdong Provincial Key Laboratory of Medicinal Lipids Zhongshan Medical College Sun Yat-sen University Guangzhou Pharmaceutical Holdings Limited National Engineering Research Center of Pharmaceutical Processing Technology of Traditional Chinese Medicine and Drug Innovation

OBJECTIVE: To investigate the effect of Ganoderma Lucidum Spore Oil(GLSO) on the tumor growth and survival of H22 tumor-bearing mice treated with cyclophosphamide(CTX), and explore the underlying mechanism.METHODS: Allograft H22 hepatocellular carcinoma mouse model was applied to investigate the effect of GLSO on the tumor growth and survival of animals, and Kaplan-Meier survival analysis was used to analyze the life span. Plasma biochemical examination was used to determine the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST), urea(UREA) and creatinine(CRE). Western blot analysis was performed to detect Programmed Death-1(PD-1), Programmed Death Ligand 1(PD-L1), Janus Kinase 2(JAK2),phosphorylated Signal Transducer and Activator of Transcription 3(p-STAT3), and Signal Transducer and Activator of Transcription 3(STAT3) expression. RESULTS: GLSO increased the anti-tumor effect of CTX and prolonged the survival of H22 tumor-bearing mice treated with CTX. Meanwhile, GLSO increased the thymus index and showed no obvious toxicity to liver functions of animals. GLSO also decreased the level of UREA in H22 tumor-bearing mice treated with CTX. Furthermore, GLSO could inhibit the expression of PD-1 in spleen, which was independent of JAK2 expression and STAT3 phosphorylation. However, GLSO did not affect the expression of PD-L1, JAK2, and p-STAT3 in tumor tissue. CONCLUSION: GLSO could strengthen the anti-tumor effect of CTX and prolong the life span of H22 tumorbearing mice, while the underlying mechanism might be relevant to the amelioration effect of thymus function and inhibition of PD-1 expression in spleen. Furthermore, these findings implied the promising role of GLSO in combination with CTX to extend the survival of patients in clinical chemotherapy of hepatocellular carcinoma.

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Sciatic nerve repair using adhesive bonding and a modified conduit

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Neural Regeneration Research 2014年第6期9卷 594-601页

作者： Xiangdang Liang hongfei cai Yongyu Hao Geng Sun Yaoyao Song Wen Chen Department of Orthopedics General Hospital of Chinese PLA Wei Zikeng Clinic of General Armament Department of Chinese PLA Department of Orthopedics 252 Hospital of Chinese PLA

When repairing nerves with adhesives, most researchers place glue directly on the nerve stumps, but this method does not fix the nerve ends well and allows glue to easily invade the nerve ends. In this study, we established a rat model of completely transected sciatic nerve injury and re- paired it using a modified 1 cm-length conduit with inner diameter of 1.5 mm. Each end of the cylindrical conduit contains a short linear channel, while the enclosed central tube protects the nerve ends well Nerves were repaired with 2-octyl-cyanoacrylate and suture, which complement the function of the modified conduit. The results demonstrated that for the same conduit, the av- erage operation time using the adhesive method was much shorter than with the suture method. No significant differences were found between the two groups in sciatic function index, motor evoked potential latency, motor evoked potential amplitude, muscular recovery rate, number of medullated nerve fibers, axon diameter, or medullary sheath thickness. Thus, the adhesive method for repairing nerves using a modified conduit is feasible and effective, and reduces the operation time while providing an equivalent repair effect.

关键词： nerve regeneration nerve repair adhesive anastomosis cyanoacrylate nerve conduits sciatic nerve electrophysiology muscle recovery the International Technology Cooperation Program neural regeneration

Ganoderma lucidum spore oil(GLSO),a novel antioxidant,extends the average life span in Drosophila melanogaster

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Food Science and Human Wellness 2021年第1期10卷 38-44页

作者： Yi Zhang hongfei cai Zhu Tao Cheng Yuan Zhaojian Jiang Juyan Liu Hiroshi Kurihara Wendong Xu Institute of Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Postdoctoral Research Station of Guangzhou Pharmaceutical Holdings Limited Guangdong 510130China Institute of National Engineering Research Center for Modernization of Extraction and Separation Process of Traditional Chinese Medicine Hanfang Pharmaceutical Co.LtdGuangdong 510240China Guangzhou Pharmaceutical Holdings Ltd Guangdong 510130China Institute of Traditional Chinese Medicine and Natural Products Jinan University601Huangpu Avenue WestGuangzhou510632China

In ancient China,Ganoderma lucidum was believed to be a medical fungus that could increase lifespan.Recently,pharmacologic studies have found that polysaccharide peptides and triterpenoids extracted from Ganoderma lucidum have various physiological effects as active compounds.However,the effects of spore oil isolated from Ganoderma lucidum remains unknown.In this study,the biological effects of Ganoderma lucidum spore oil(GLSO)were evaluated using a Drosophila melanogaster model.Compared with untreated groups,groups treated with GLSO had significantly longer average and maximum lifespan in both normal conditions and under oxidative stress.The activities of various antioxidant enzymes were measured to determine the antioxidant effect of GLSO.GLSO treatment markedly enhanced total superoxide dismutase(SOD)and catalase(CAT)activity and decreased levels of malondialdehyde(MDA).Further,we found dose-dependent increases in the mRNA expression of Cu,Zn-SOD,Mn-SOD,and CAT in GLSO-treated groups.These results suggest that GLSO may effectively eliminate free radicals and extend lifespan in Drosophila.Future work should investigate the value of GLSO as a functional food for the prevention of aging in larger animal models.

关键词： Ganoderma lucidum spore oil Antioxidant activity Drosophila melanogaster Anti-aging

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光伏直流配电故障诊断及火灾风险评估技术

全球能源互联网 2024年第1期7卷 46-54页

作者：宋振宇邢晨李明张文煜才鸿飞乔颖姚芳国网冀北张家口风光储输新能源有限公司河北省张家口市075000 清华大学电机工程与应用电子技术系北京市海淀区100084 河北工业大学电气工程学院天津市北辰区300130

长期服役的光伏发电系统直流配电故障概率高、火灾风险及危害大,严重影响光伏电站的运行安全、发电与出力能力,并对电站运维提出故障与火灾精准检测的技术挑战。为此,从生产现场直流配电柜故障分析入手,揭示接触不良、绝缘劣化、故障电... 详细信息

长期服役的光伏发电系统直流配电故障概率高、火灾风险及危害大,严重影响光伏电站的运行安全、发电与出力能力,并对电站运维提出故障与火灾精准检测的技术挑战。为此,从生产现场直流配电柜故障分析入手,揭示接触不良、绝缘劣化、故障电弧、电气火灾的演变机理;分析国内外故障电弧诊断与火灾检测技术的理论成果应对在运电站电弧火灾的有效性,提出故障电弧预诊断与火灾检测技术的应用解决方案。研究结果表明,提出的故障电弧诊断与火灾检测技术应用方案,弥补直流继电保护对串联电弧故障无法保护的不足,能够有效诊断故障电弧并检测烟雾和高温,避免火灾及其扩大事故的发生。

关键词：光伏火灾直流配电柜故障机理电弧检测技术

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青少年百草枯中毒后肺移植1例并文献复习

器官移植 2024年第1期15卷 112-117页

作者：孟凡杰张岩蔡宏飞孟凡宇王瑞崔有斌陈静瑜李洋吉林大学白求恩第一医院胸外科长春130021 南京医科大学附属无锡人民医院肺移植中心

目的总结百草枯中毒患者中毒后移植时机以及相关处理措施对预后的影响。方法回顾性分析1例百草枯中毒行双肺移植术治疗患者的临床资料,总结分析该例患者的临床表现、辅助检查以及诊治经过。结果1例17岁青少年在摄入25%百草枯20~30 mL后... 详细信息

目的总结百草枯中毒患者中毒后移植时机以及相关处理措施对预后的影响。方法回顾性分析1例百草枯中毒行双肺移植术治疗患者的临床资料,总结分析该例患者的临床表现、辅助检查以及诊治经过。结果1例17岁青少年在摄入25%百草枯20~30 mL后出现恶心、呕吐、咳嗽伴全身乏力入院。患者经对症支持治疗后,氧饱和情况无改善,肺部纤维化持续进展,遂在体外膜肺氧合(ECMO)辅助下行序贯双侧肺移植。经术后康复治疗,并积极防治并发症,患者于术后50 d出院。结论百草枯中毒后的移植时机可选择在肝肾功能开始恢复时,围手术期主动、有针对性地预防潜在致病性细菌感染,以及早期康复训练有助于改善肺移植受者的预后。

关键词：肺移植百草枯中毒肺纤维化呼吸衰竭体外膜肺氧合感染过客淋巴细胞综合征支气管狭窄

不同干燥方式对灵芝孢子粉中灵芝孢子油过氧化值的影响

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广东化工 2024年第1期51卷 28-30页

作者：蔡鸿飞张琴关伟键杨阳袁诚许文东广州白云山汉方现代药业有限公司中药制药过程技术与新药创制国家工程研究中心广东省药用脂质重点实验室广东广州510240 广药汉方(珠海横琴)药业有限公司广东珠海519000 西藏广药汉方灵芝产业有限公司西藏林芝860000

目的:比较不同干燥方式对灵芝孢子粉中灵芝孢子油过氧化值的影响。方法:以西藏灵芝孢子粉为例,采用不同的干燥方式将灵芝孢子粉干燥至合格,然后进行破壁,用超临界CO_(2)萃取法提取灵芝孢子油,检测灵芝孢子油的过氧化值,并比较不同干燥... 详细信息

目的:比较不同干燥方式对灵芝孢子粉中灵芝孢子油过氧化值的影响。方法:以西藏灵芝孢子粉为例,采用不同的干燥方式将灵芝孢子粉干燥至合格,然后进行破壁,用超临界CO_(2)萃取法提取灵芝孢子油,检测灵芝孢子油的过氧化值,并比较不同干燥方式对灵芝孢子油的过氧化值的影响。结果:微波干燥和真空干燥方式得到的灵芝孢子油过氧化值均合格,分别为0.13%,0.14%;热风干燥和晾晒干燥方式得到的灵芝孢子油过氧化值不合格,分别为0.16%、0.17%。结论:水分变化率可能是灵芝孢子粉干燥过程中的重要影响因素,微波干燥方式水分变化率远高于其他方式,干燥效率高,耗能少,且不影响孢子油过氧化值,适合干燥大规模样品;其它干燥方式相对比较费时耗能,且灵芝孢子粉存在变质的风险。

关键词：灵芝孢子粉干燥方式灵芝孢子油过氧化值

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传统特色藏药藏荆芥(萨都那保)的研究进展

山东化工 2024年第10期53卷 121-124,127页

作者：毛禹康张琴蔡鸿飞兰小中李康强杨阳许文东中药制药过程技术与新药创制国家工程研究中心广东广州510240 广州白云山汉方现代药业有限公司广东广州510240 西藏农牧学院西藏林芝860000

藏荆芥作为民族特色明显、应用历史悠久的西藏地区独有药材,含萜类、酯类、酮类等多种成分,传统应用于癫痫抽搐、神昏惊厥等症,现代研究表明其具有抗炎、镇痛、心脑血管保护、肾保护等广泛药理活性,开发潜力显著。然而,目前藏荆芥的研... 详细信息

藏荆芥作为民族特色明显、应用历史悠久的西藏地区独有药材,含萜类、酯类、酮类等多种成分,传统应用于癫痫抽搐、神昏惊厥等症,现代研究表明其具有抗炎、镇痛、心脑血管保护、肾保护等广泛药理活性,开发潜力显著。然而,目前藏荆芥的研究开发受限于地理位置、药材资源等多种因素。本文查阅、分析并归纳藏荆芥相关文献和专著,对藏荆芥的药材资源、化学成分、药理活性及质量控制等相关研究进行总结和综述,并对后续研究方向进行展望,旨在为藏荆芥的进一步研究开发提供参考,积极促进民族医药发展。

关键词：藏荆芥资源分布化学成分药理活性质量标准

HPLC法测定藏荆芥挥发油中荆芥内酯特征成分含量的方法学研究

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广东化工 2024年第2期51卷 35-37页

作者：蔡鸿飞张琴毛禹康韩亚明杨阳张圳李康强许文东广州白云山汉方现代药业有限公司中药制药过程技术与新药创制国家工程研究中心广东广州510240 广药汉方(珠海横琴)药业有限公司广东珠海519000

目的:建立HPLC法测定藏荆芥挥发油中E,Z-荆芥内酯特征成分含量的方法。方法:以十八烷基硅烷键合硅胶为填充剂(色谱柱:Supersil ODS 250 mm×4.6 mm,5μm),以乙腈-水(40∶60)为HPLC的流动相,流速为1 mL/min,色谱柱的柱温为30℃,检测波长... 详细信息

目的:建立HPLC法测定藏荆芥挥发油中E,Z-荆芥内酯特征成分含量的方法。方法:以十八烷基硅烷键合硅胶为填充剂(色谱柱:Supersil ODS 250 mm×4.6 mm,5μm),以乙腈-水(40∶60)为HPLC的流动相,流速为1 mL/min,色谱柱的柱温为30℃,检测波长为216 nm。结果:线性关系方程为y=1E+07x+287501,R2=0.9991,在0.0098~2.4405 mg/mL范围内的峰面积与进样浓度线性关系良好。精密度和重复性良好,峰面积RSD分别为0.66%、0.32%,加样回收率平均值为101.67%,24 h内样品溶液稳定。结论:首次确认藏荆芥挥发油中含有E,Z-荆芥内酯,所建立的高效液相色谱法方法简单、准确、快速,重复性强,专属性好,可应用于藏荆芥E,Z-荆芥内酯特征成分的含量测定。

关键词：藏荆芥 E,Z-荆芥内酯含量测定高效液相色谱方法学

Experimental study on new self and mutual-aiding occlusive dressing for wound

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Chinese Medical Journal 2014年第7期127卷 1321-1327页

作者： Liang Xiangdang Chen Wen Sun Geng Liu Shiluan cai hongfei Zhou Lin Orthopedics Department Chinese People's Liberation Army General Hospital Beijing 100853 China Orthopedics Department No.252 Hospital of People's Liberation Army Baoding Hebei 071000 China

Background Self and mutual-aiding occlusive dressing is a novel method to treat with the wounds in special circumstances.This study aims to prepare a new antimicrobial adhesive for the dressing and evaluate the application effects of the adhesive.Methods The main component of the new antimicrobial adhesive was 5％ triclosan / cyanoacrylate （CA） antimicrobial adhesive.The adhesive was modified with carboxylic multi-walled carbon nanotubes （MWCNTs-COOH）,multi-walled carbon nanotubes （MWCNTs）,hydrophobic nano-silica,nitrile rubber,epoxy resin and polymethyl methacrylate （PMMA） respectively.The bond strength,toughness and viscosity of the modified adhesive in different concentrations were examined to select the optimal modifying material and the best ratio to prepare the new antimicrobial adhesive according to the results.After that,the antimicrobial property of the new antimicrobial adhesive was tested by filter paper method.At last,we disposed the injury models in rats using the new antimicrobial adhesive to examine the application effects.Results In individual tests,the bond strength modification performance of 0.064％ MWCNTS-COOH is the best,the bond strength is （14.71±1.48） Mpa.8％ nano-silica shows the best toughness modification performance,the Tg is （1.10±0.24）℃.The viscosity modification performance of 8％ nano-silica is the best,the viscosity is （15 536.68±28.4） cP.However,consolidating three test results,6％ nano-silica / antimicrobial adhesive has the balanced bond strength,toughness and viscosity.Its bond strength is （14.03±1.92） Mpa,the Tg is （3.60±0.68）℃,and the viscosity is （5 278.87±31.68） cP.The inhibition zone diameter of 6％ nano-silica / antimicrobial adhesive and antimicrobial adhesive group in Day 5 is （28.61±0.91） mm versus （28.24±2.69） mm （P ＞0.05）.In animal studies,both in blood routine test and pathological section,6％ nano-silica / antimicrobial adhesive group shows lower white blood cells count than gauze bandage group （P ＜0.05）.Conclusions 6％ nano-silica has the optimal effect of bond strength

关键词： antimicrobial adhesive cyanoacrylate modification optimization nanomaterial