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Journal of Environmental Sciences 2019年第11期31卷 168-176页

作者： Jianhua Guo Changzhou Yan Zhuanxi Luo hongda fang Shugang Hu Yinglan Cao Key Laboratory of Urban Environment and Health Institute of Urban Environment Chinese Academy of Sciences Xiamen 361021 China University of Chinese Academy of Sciences Beijing 100049 China College of Food and Biological Engineering Jimei University Xiamen 361021 China College of Chemical and Environmental Engineering Shandong University of Science and Technology Qingdao 266590 China

Cadmium (Cd) and arsenic (As) are two of the most toxic ***,the chemical behaviors of these two elements are different,making it challenging to utilize a single adsorbent with high adsorption capacity for both Cd(Ⅱ) and As(Ⅴ) *** solve this problem,we synthesized HA/Fe-Mn oxides-loaded biochar (HFMB),a novel ternary material,to perform this task,wherein scanning electron microscopy (SEM) combined with EDS (SEM-EDS) was used to characterize its morphological and physicochemical *** maximum adsorption capacity of HFMB was 67.11 mg/g for Cd(Ⅱ) and 35.59 mg/g for As(Ⅴ),which is much higher compared to pristine biochar (11.06 mg/g,0 mg/g for Cd(Ⅱ) and As(Ⅴ),respectively).The adsorption characteristics were investigated by adsorption kinetics and the effects of the ionic strength and pH of solutions.X-ray photoelectron spectroscopy (XPS) and Fourier-transform infrared spectroscopy (FT-IR) revealed that chelation and deposition were the adsorption mechanisms that bound Cd(Ⅱ) to HFMB,while ligand exchange was the adsorption mechanism that bound As(Ⅴ).

关键词： Humic acid Fe-Mn oxides Biochar Adsorption Cd(Ⅱ) As(Ⅴ)

Characterization of the chemical fungicides-responsive and bacterial pathogen-preventing Bacillus licheniformis in rice spikelet

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Food Quality and Safety 2023年第2期7卷 256-266页

作者： Chengfang Zhan Mengchen Wu hongda fang Xiaoyu Liu Jiuyue Pan Xiaoyan Fan Mengcen Wang Haruna Matsumoto State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects Zhejiang UniversityHangzhouChina Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of Pesticide and Environmental ToxicologyCollege of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina Zhejiang Laboratory for Systems&Precision Medicine Zhejiang University Medical CenterHangzhouChina School of Medicine Zhejiang UniversityHangzhouChina College of Plant Protection Hunan Agricultural UniversityHunanChina Plant Genetics TUM School of Life SciencesTechnical University of MunichFreisingGermany Global Education Program for AgriScience Frontiers Graduate School of AgricultureHokkaido UniversitySapporoJapan

Seed-borne bacterial pathogens cause severe yield loss and biotoxin contamination in rice,leading to increasing concern on the global food supply and environmental *** native microbes play an important role in defending against diseases,but their actions are often influ-enced by the chemical fungicides applied in the ***,Bacillus licheniformis mmj was isolated from rice spikelet,which uniquely showed not only fungicide-responsiveness but also broad-spectrum antimicrobial activity against major rice bacterial pathogens including Xanthomonas oryzae ***,Burkholderia plantari and Burkholderia *** understand the hallmark underlying the environmental adaptation and anti-microbial activity of *** mmj,the genome sequence was determined by SMRT and subjected to bioinformatics *** sequence analysis enabled the identification of a set of antimicrobial-resistance and antibacterial activity genes together with an array of harsh environment-adaptive ***,*** mmj metabolites were analyzed with gas chromatography coupled to triple quadrupole mass spectrometry,and the volatile components that were linked with the antimicrobial activity were preliminarily ***,the present findings reveal the genomic and metabolic landscapes underlying fungicide-responsive ***,which offers a new opportunity to design harsh environment-adaptive biopesticides to cope with prevalent bacterial phytopathogens.

关键词： Bacillus licheniformis fungicide adaptation antimicrobial activity genomics metabolites rice production

Early surveillance of rice bakanae disease using deep learning and hyperspectral imaging

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aBIOTECH 2024年第3期5卷 281-297页

作者： Sishi Chen Xuqi Lu hongda fang Anand Babu Perumal Ruyue Li Lei Feng Mengcen Wang Yufei Liu College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China State Key Laboratory of Rice Biology and Breeding Zhejiang UniversityHangzhou 310058China Computational Modeling and Nanoscale Processing Unit National Institute of Food TechnologyEntrepreneurship and Management-ThanjavurMinistry of Food Processing IndustriesThanjavur 613005India College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310058China Global Education Program for AgriScience Frontiers Graduate School of AgricultureHokkaido UniversitySapporo 060-8589Japan

Bakanae disease,caused by Fusarium fujikuroi,poses a significant threat to rice production and has been observed in most rice-growing *** disease symptoms caused by different pathogens may vary,including elongated and weak stems,slender and yellow leaves,and dwarfism,as *** disease is likely to cause necrosis of diseased seedlings,and it may cause a large area of infection in the field through the transmission of ***,early disease surveillance plays a crucial role in securing rice *** monitoring methods are both time-consuming and labor-intensive and cannot be broadly *** this study,a combination of hyperspectral imaging technology and deep learning algorithms were used to achieve in situ detection of rice seedlings infected with bakanae *** data were obtained on the 9th,15th,and 21st day after rice infection to explore the physiological and biochemical performance,which helps to deepen the research on the disease *** data were obtained over these same periods of infection,and a deep learning model,named Rice Bakanae Disease-Visual Geometry Group(RBD-VGG),was established by leveraging hyperspectral imaging technology and deep learning *** on this model,an average accuracy of 92.2%was achieved on the 21st day of *** also achieved an accuracy of 79.4%as early as the 9th *** characteristic wavelengths were extracted to increase the feasibility of using portable spectral equipment for field ***,the model offers an efficient and non-destructive surveillance methodology for monitoring bakanae disease,thereby providing an efficient avenue for disease prevention and control.

关键词： Bakanae disease Hyperspectral imaging Deep learning Early surveillance Disease monitoring

Specific biotests to assess eco-toxicity of biodegradable polymer materials in soil

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Journal of Environmental Sciences 2021年第7期33卷 150-162页

作者： Haiyan Chen Fei Wang Huilun Chen hongda fang Weiying Feng Yuan Wei FanFan Wang HaiLei Su YiDong Mi Min Zhou XinRu Li Serena Doni Andrea Corti State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental ScienceBeijing 10012China School of Energy&Environmental Engineering University of Science and Technology BeijingBeijing 100083China Water Treatment Engineering Research Center of Jimei University Fujian 361021China School of Space and Environment Beihang UniversityBeijing 100191China Beijing Advanced Innovation Center for Big Data-Based Precision Medicine Beihang UniversityBeijing 100191China Eco-toxicology Laboratory National Research Council(CNR)-Institute of Ecosystem Study(ISE)Via Moruzzi 1Pisa 56124Italy INSTM Unit Department of Chemistry and Industrial ChemistryUniversity of Pisavia Risorgimento 3556126Italy BIOlabvia Vecchia Livornese 1291 Loc.S.Piero a Grado(Pi)Pisa 56122Italy

Eco-toxicity investigation of polymer materials was considered extremely necessary for their potential menace,which was widely use as mulching materials in *** this study,polyethylene(PE),polystyrene(PS)and synthetic biomaterials-Ecoflex and cellulose were applying into soil cultivated with two potential indicator plants species:oat(A v ena sati v a)and red radish(Raphanus sativum).Variety of chemical,biochemical parameters and enzyme activity in soil were proved as effective approach to evaluate polymers phytotoxicity in plant-soil *** F-value of biomass,pH,heavy metal and electoral conductivity of Raphanus behaved significant different from *** analysis results indicated biodegradation was fast in PE than PS,besides,heavy metals were dramatically decrease in the end implied the plant absorption may help decrease heavy metal *** increase value at T2 of Dehydrogenase activity(0.84 higher than average value for Avena&0.91 higher for Raphanus),Metabolic Index(3.12 higher than average value for Avena&3.81 higher for Raphanus)means during soil enzyme activity was promoted by biodegradation for its heterotrophic organisms’energy transportation was *** analysis was carried on Biplot PC1(24.2%of the total variance),PC2(23.2%of the total variance),versus PC3(22.8%of the total variance),which indicated phosphatase activity and metabolic index was significant correlated,and high correlation of ammonium and protease ***,the effects were more evident in Raphanus treatments than in Avena,suggesting the higher sensitivity of Raphanus to polymers treatment,which indicate biodegradation of polymers in Raphanus treatment has produced intermediate phytotoxic compounds.

关键词： Avena sativa Raphanus sativum Polymer materials Biodegradation Eco-toxicity Phytotoxicity

Gut pathobiome mediates behavioral and developmental disorders in biotoxin-exposed amphibians

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Environmental Science and Ecotechnology 2024年第5期21卷 208-218页

作者： Qianqian Pan Tianxing Lv Haorong Xu hongda fang Meng Li Jiaping Zhu Yue Wang Xiaoyan Fan Ping Xu Xiuguo Wang Qiangwei Wang Haruna Matsumoto Mengcen Wang Ministry of Agricultural and Rural Affairs Laboratory of Molecular Biology of Crop Pathogens and Insects Zhejiang UniversityHangzhou310058China Institute of Pesticide and Environmental Toxicology College of Agriculture and BiotechnologyZhejiang UniversityHangzhou310058China Institution of Tea Science Zhejiang UniversityHangzhou310058China The Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdao266101China Global Education Program for AgriScience Frontiers Graduate School of AgricultureHokkaido UniversitySapporoJapan

Emerging evidence suggests a link between alterations in the gut microbiome and adverse health outcomes in the hosts exposed to environmental ***,the causal relationships and underlying mechanisms remain largely *** we show that exposure to biotoxins can affect gut pathobiome assembly in amphibians,which in turn triggers the toxicity of exogenous *** used Xenopus laevis as a model in this *** exposed to tropolone demonstrated notable developmental impairments and increased locomotor activity,with a reduction in total length by 4.37%e22.48%and an increase in swimming speed by 49.96%e84.83%.Fusobacterium and Cetobacterium are predominant taxa in the gut pathobiome of tropolone-exposed *** tropolone-induced developmental and behavioral disorders in the host were mediated by assembly of the gut pathobiome,leading to transcriptome *** study not only advances our understanding of the intricate interactions between environmental pollutants,the gut pathobiome,and host health but also emphasizes the potential of the gut pathobiome in mediating the toxicological effects of environmental contaminants.

关键词： Gut microbiome Pathobiome Biotoxin pollution Developmental toxicity Behavioral disorders

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大亚湾表层沉积物中磷的形态分布特征

中山大学学报（自然科学版） 2010年第6期49卷 126-131页

作者：何桐谢健余汉生方宏达国家海洋局南海海洋工程勘察与环境研究院广东广州510300 国家海洋局南海环境监测中心广东广州510300

沉积物中不同形态的磷具有不同的生物有效性和地球化学行为,它们的含量和分布特征包含着许多环境地球化学信息。因此,对沉积物中磷的地球化学形态的研究具有重要的环境地球化学意义。采用SEDEX法将大亚湾表层沉积物中不同形态的磷分离开... 详细信息

沉积物中不同形态的磷具有不同的生物有效性和地球化学行为,它们的含量和分布特征包含着许多环境地球化学信息。因此,对沉积物中磷的地球化学形态的研究具有重要的环境地球化学意义。采用SEDEX法将大亚湾表层沉积物中不同形态的磷分离开,继而分析表层沉积物中不同形态磷的含量、分布特征及其影响因素。结果表明,表层沉积物中总磷的含量较高,平均高达341.87 mg/kg;无机磷所占的比例很高,平均达85.11%,平均含量为290.81 mg/kg;有机磷的平均含量为51.05 mg/kg。表层沉积物中的碎屑态磷是无机磷的主要赋存形态,平均占无机磷的47.67%;其次为自生磷灰石及钙结合态磷,平均占无机磷的34.79%;铁结合态磷和吸附态磷分别仅占无机磷中的10.23%和7.31%。其中,潜在的生物有效性磷(包括Ads-P、Fe-P和OP)平均占总磷的29.40%。在空间分布上,各站间不同形态磷的含量差异与污染物的陆源输入、沉积物性质等因素有关。

关键词：磷形态分布特征生物有效性磷表层沉积物大亚湾

大亚湾表层沉积物间隙水与上覆水中营养盐分布特征

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环境科学学报 2008年第11期28卷 2361-2368页

作者：何桐谢健余汉生方宏达高全洲中山大学地理科学与规划学院广州510275 国家海洋局南海海洋工程勘察与环境研究院广州510300 国家海洋局南海环境监测中心广州510300

通过2007年4月和8月2次对大亚湾内13个站位的采样分析,探讨了表层沉积物间隙水和上覆水中营养盐含量的时空分布特征,估算了沉积物-海水界面营养盐的扩散通量,并对间隙水和上覆水中的营养盐含量进行了相关性分析.结果表明,大部分表层沉... 详细信息

通过2007年4月和8月2次对大亚湾内13个站位的采样分析,探讨了表层沉积物间隙水和上覆水中营养盐含量的时空分布特征,估算了沉积物-海水界面营养盐的扩散通量,并对间隙水和上覆水中的营养盐含量进行了相关性分析.结果表明,大部分表层沉积物间隙水中营养盐含量远高于上覆水,但两者不具有相同的含量分布趋势.PO43--P、SiO23--Si、NH4+-N、NO2--N和NO3--N在沉积物-海水界面的平均扩散通量分别为9.22、444.99、13.49、20.71、8.99μmol.m-.2d-1.相关性分析表明,营养盐在间隙水和上覆水中的含量均无明显相关性,说明间隙水中赋存营养盐的浓度并不是其上覆水中营养盐含量的决定因素.

关键词：间隙水上覆水营养盐扩散通量大亚湾

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轻症急性胰腺炎的临床诊治

中国药物经济学 2015年第4期10卷 140-141页

作者：方大鑫方宏达苏州九龙医院江苏苏州215000

目的探讨轻症急性胰腺炎的临床诊治。方法收集2011年6月至2014年8月我院收治的100例轻症急性胰腺炎患者资料,按随机数字表法将患者分为试验组和对照组,各50例。对照组患者给予综合治疗,试验组患者在此基础上应用乌司他丁治疗。比较两组... 详细信息

目的探讨轻症急性胰腺炎的临床诊治。方法收集2011年6月至2014年8月我院收治的100例轻症急性胰腺炎患者资料,按随机数字表法将患者分为试验组和对照组,各50例。对照组患者给予综合治疗,试验组患者在此基础上应用乌司他丁治疗。比较两组患者的治疗效果。结果两组患者均治愈出院,且试验组患者的体温恢复正常时间、淀粉酶恢复正常时间、住院时间均明显少于对照组,差异均有统计学意义(均P<0.05)。结论在综合治疗的基础上应用乌司他丁治疗轻症急性胰腺炎,效果较好。

关键词：轻症急性胰腺炎乌司他丁临床诊治

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阿莫西林结合果胶铋治疗胃炎患者临床分析

中外医疗 2015年第20期34卷 115-116页

作者：方大鑫方宏达苏州九龙医院江苏苏州215000 南通大学医学院消化内科江苏南通226001

目的探讨阿莫西林结合果胶铋治疗胃炎患者的临床效果。方法随机选取了该院2011年10月—2013年10月期间来治疗的60例胃炎患者患者作为研究对象,利用计算机软件把60例患者随机分成两组,观察组和对照组,每组分别30例患者。给予对照组克拉... 详细信息

目的探讨阿莫西林结合果胶铋治疗胃炎患者的临床效果。方法随机选取了该院2011年10月—2013年10月期间来治疗的60例胃炎患者患者作为研究对象,利用计算机软件把60例患者随机分成两组,观察组和对照组,每组分别30例患者。给予对照组克拉霉素结合果胶铋治疗,观察组使用阿莫西林结合果胶铋治疗,比较观察两组治疗效果。结果观察组治疗有效率为93.3%,对照组治疗有效率为76.6%,观察组治疗效果明显优于对照组。两组差异有统计学意义(P<0.05)。结论使用阿莫西林结合果胶铋治疗胃炎患者临床效果好,值得临床推广。

关键词：阿莫西林果胶铋胃炎患者临床分析

The Remote Sensing of Sargassum on Daya Bay

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遥感信息 1990年第A2期12卷 25-27页

作者： Li Tiefang (Center for Remote Sensing, Zhongshan University) Liu Huai, fang hongda (South China Sea Marine Environmental Monitoring Center, SOA) Dong Yuguo (Guangzhou Institute of New Techniques in Geology, Academic Sinica)

There are a lots of sargassum growing on the sea area of Daya Bay. The sargassum is a type of big algae with body length about 1～2 meters and the longest about 5～6 meters. The sargassum begins to grow in Fall of every year, breaks in April or May of the next year and floats away with current. It is the major brigade material to the cooling system pipe of Daya Bay Nuclear Power Station. So, it’s needed to investigate its culture regularity, distribution, productions and floating quantity toward the cooling system pipe on the Bay. As the Bay area is very wide, it’s quite difficult to determine the distribution range and the total production only depended upon on site investigation. Remote sensing investigation, is a very effective method.

关键词：二衬