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Enhancing m6A modification in the motor cortex facilitates corticospinal tract remodeling after spinal cord injury

Neural Regeneration Research 2025年第6期 1749-1763页

作者： Tian Qin Yuxin jin Yiming Qin Feifei Yuan hongbin Lu Jianzhong Hu Yong Cao Chengjun Li Department of Spine Surgery and Orthopedics Xiangya Hospital Central South University Key Laboratory of Organ Injury Aging and Regenerative Medicine of Hunan Province National Clinical Research Center for Geriatric Disorders Xiangya Hospital Central South University Department of Sports Medicine Xiangya Hospital Central South University

Spinal cord injury typically causes corticospinal tract disruption. Although the disrupted corticospinal tract can self-regenerate to a certain degree, the underlying mechanism of this process is still unclear. N6-methyladenosine（m6A） modifications are the most common form of epigenetic regulation at the RNA level and play an essential role in biological processes. However, whether m6A modifications participate in corticospinal tract regeneration after spinal cord injury remains unknown. We found that expression of methyltransferase 14 protein（METTL14） in the locomotor cortex was high after spinal cord injury and accompanied by elevated m6A levels. Knockdown of Mettl14 in the locomotor cortex was not favorable for corticospinal tract regeneration and neurological recovery after spinal cord injury. Through bioinformatics analysis and methylated RNA immunoprecipitation-quantitative polymerase chain reaction, we found that METTL14 regulated Trib2 expression in an m6A-regulated manner, thereby activating the mitogen-activated protein kinase pathway and promoting corticospinal tract regeneration. Finally, we administered syringin, a stabilizer of METTL14, using molecular docking. Results confirmed that syringin can promote corticospinal tract regeneration and facilitate neurological recovery by stabilizing METTL14. Findings from this study reveal that m6A modification is involved in the regulation of corticospinal tract regeneration after spinal cord injury.

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The second polar body contributes to the fate asymmetry in the mouse embryo

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National Science Review 2022年第7期9卷 32-42页

作者： hongbin jin Yang Han Huasong Wang J.Xiao He Li Weimin Shen Lin Zhang Luxi Chen Shunji Jia Ping Yuan Hui Chen Anming Meng Laboratory of Molecular Developmental Biology State Key Laboratory of Membrane BiologyTsinghua-Peking Center for Life Sciences School of Life SciencesTsinghua University Center for Reproductive Genetics and Reproductive Medicine Sun Yat-sen Memorial HospitalSun Yat-sen University Guangzhou Laboratory

The polar bodies(PBs) are extruded microcells during oocyte meiosis and generally regarded as inessentials for embryonic development.Therefore,PBs have been widelyused as important materials for pre-implantation genetic diagnosis in human.Here we report that the second PB(PB2) in the mouse zygote may play roles in cell-fate specification and post-implantation development.A subset of mRNAs encoding pluripotency-related factors are enriched in PB2.Nascent proteins maybe synthesized in PB2 after fertilization and transport from PB2 to the zygote before the two-cell stage.The PB2-attached blastomere(pbB) at the two-cell stage,compared to the other blastomere(npbB),likely contributes more descendants to the inner cell mass(ICM) lineage in the blastocyst.Removal of PB2 from the zygote or transient blockage of material exchange between PB2 and the zygote by nocodazole treatment appears to cause a loss of the ICM fate bias of pbB.PB2 removal or nocodazole treatment also results in abnormal post-implantation development.Injection of PB2 lysate into pbB of PB2-removed two-cell-stage embryos may reset the cell-fate preference and rescue post-implantation development.Our data collectively suggest that PB2 would demarcate the earliest cell-fate asymmetry of the mouse zygote and be required for post-implantation development.

关键词： mouse embryo polar body cell fate asymmetry

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Evidence fusion procedure based on hybrid DSm model

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Journal of Systems Engineering and Electronics 2014年第6期25卷 959-967页

作者： Hongfei Li hongbin jin Kangsheng Tian Air Force Early Warning Academy

Dezert-Smarandache（DSm） theory, a new information fusion theory, is widely applied in image processing, multiple targets tracking identification, and other areas for its excellent processing ability of imperfect information. However, earlier research on DSm theory mainly focused on one sort of questions. An evidence fusion procedure is proposed based on the hybrid DSm model to compensate for a lack of research on the entire information procedure of DSm theory. This paper analyzes the evidence fusion procedure, as well as correlative node input and output information. Key steps and detailed procedures of evidence fusion are also discussed. Finally, an experiment illustrates the efficiency of the proposed evidence fusion procedure.

关键词： Dezert-Smarandache（DSm） theory,evidence fusion procedure,hybrid DSm model,information fusion

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基于BERT的双特征融合注意力的方面情感分析模型

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计算机科学与探索 2024年第1期18卷 205-216页

作者：李锦夏鸿斌刘渊江南大学人工智能与计算机学院江苏无锡214122 江苏省媒体设计与软件技术重点实验室江苏无锡214122

方面情感分析旨在预测句子或文档中一个特定方面的情感极性,现阶段大部分的研究都是使用注意力机制对上下文进行建模。然而,目前情感分类模型在使用BERT模型计算表征之间的依赖关系抽取特征时,大多未根据不同的语境背景考虑上下文信息,... 详细信息

方面情感分析旨在预测句子或文档中一个特定方面的情感极性,现阶段大部分的研究都是使用注意力机制对上下文进行建模。然而,目前情感分类模型在使用BERT模型计算表征之间的依赖关系抽取特征时,大多未根据不同的语境背景考虑上下文信息,导致建模后的特征缺乏上下文的语境信息。同时,方面词的重要性未能得到充分的重视而影响模型整体分类的性能。针对上述问题,提出双特征融合注意力方面情感分析模型(DFLGA-BERT),分别设计了局部与全局的特征抽取模块,充分捕捉方面词和上下文的语义关联。并将一种改进的“准”注意力添加到DFLGA-BERT的全局特征抽取器中,使模型学习在注意力的融合中使用减性注意力以削弱噪声产生的负面影响。基于条件层规泛化(CLN)设计了局部特征和全局特征的特征融合结构来更好地融合局部和全局特征。在SentiHood和SemEval 2014 Task 4数据集上进行了实验,实验结果表明,与基线模型相比该模型在融入了上下文语境特征后取得了较明显的性能提升。

关键词：情感分析自然语言理解准注意力机制上下文注意

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Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023 edition)

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Chinese Herbal Medicines 2024年第1期16卷 3-12页

作者： Qing Zhao Tong Wang hongbin Wang Peng Cao Chengyu Jiang Hongzhi Qiao Lihua Peng Xingdong Lin Yunyao Jiang Honglei jin Huantian Zhang Shengpeng Wang Yang Wang Ying Wang Xi Chen Junbing Fan Bo Li Geng Li Bifeng Liu Zhiyang Li Suhua Qi Mingzhen Zhang Jianjian Zheng Jiuyao Zhou Lei Zheng Kewei Zhao Guangdong Engineering Research Center of Chinese Herbal Vesicles The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Institute of Life and Health Engineering the First Affiliated HospitalJinan UniversityGuangzhou 510630China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210046China Institute of Basic Medicine Chinese Academy of Medical SciencesBeijing 100005China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310030China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Department of Bone and Joint Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou 510630China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAomen 999078China Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai 200233China School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515China School of Life Sciences Nanjing UniversityNanjing 210008China Nanfang Hospital Southern Medical UniversityGuangzhou 510515China Laboratory Animal Center Guangzhou University of Chinese MedicineGuangzhou 510405China College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital Nanjing 210008China School of Medical and Technology Xuzhou Medical UniversityXuzhou 221004China School of Basic Medical Sciences Xi’an Jiaotong UniversityXi’an 710065China The First Affiliated Hospital of Wenzhou Medical University WenZhou 325035China

To promote the development of extracellular vesicles of herbal medicine especially the establishment of standardization,led by the National Expert Committee on Research and Application of Chinese Herbal Vesicles,research experts in the field of herbal medicine and extracellular vesicles were invited nationwide with the support of the Expert Committee on Research and Application of Chinese Herbal Vesicles,Professional Committee on Extracellular Vesicle Research and Application,Chinese Society of Research Hospitals and the Guangdong Engineering Research Center of Chinese Herbal Vesicles.Based on the collation of relevant literature,we have adopted the Delphi method,the consensus meeting method combined with the nominal group method to form a discussion draft of“Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023)”.The first draft was discussed in online and offline meetings on October 12,14,November 2,2022 and April and May 2023 on the current status of research,nomenclature,isolation methods,quality standards and research applications of extracellular vesicles of Chinese herbal medicines,and 13 consensus opinions were finally formed.At the Third Academic Conference on Research and Application of Chinese Herbal Vesicles,held on May 26,2023,Kewei Zhao,convenor of the consensus,presented and read the consensus to the experts of the Expert Committee on Research and Application of Chinese Herbal Vesicles.The consensus highlights the characteristics and advantages of Chinese medicine,inherits the essence,and keeps the righteousness and innovation,aiming to provide a reference for colleagues engaged in research and application of Chinese herbal vesicles at home and abroad,decode the mystery behind Chinese herbal vesicles together,establish a safe,effective and controllable accurate Chinese herbal vesicle prevention and treatment system,and build a bridge for Chinese medicine to the world.

关键词： Chinese herbal medicine extracellular vesicles research application consensus

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基于神经-肌骨-外骨骼耦合仿真框架的平地和上坡行走动力学分析

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力学学报 2024年第3期56卷 817-831页

作者：靳葳刘佳奇张琦炜方虹斌复旦大学智能机器人研究院上海200433 复旦大学义乌研究院浙江义乌322000 同济大学航空航天与力学学院上海200092

评估人体穿戴外骨骼后的运动性能是指导外骨骼硬件迭代和控制策略优化的重要依据.与传统的基于实验的评估相比,基于人-外骨骼耦合系统动力学仿真的评估避免了平台搭建和实地测试、实验数据分析处理的巨大人力和时间成本.此外,如将人体... 详细信息

评估人体穿戴外骨骼后的运动性能是指导外骨骼硬件迭代和控制策略优化的重要依据.与传统的基于实验的评估相比,基于人-外骨骼耦合系统动力学仿真的评估避免了平台搭建和实地测试、实验数据分析处理的巨大人力和时间成本.此外,如将人体神经肌骨动力学纳入人-外骨骼耦合系统,动力学仿真还能够生成肌肉激活、肌肉力和关节力矩等实验中难以测量的生理学和生物力学信息,从而可以从肌肉发力的层面评估不同环境下穿戴外骨骼对人体运动的影响.本研究以人体肌骨-髋关节外骨骼耦合系统为研究对象,基于考虑人体神经肌骨动力学、足地接触和人-外骨骼交互作用的完整神经-肌骨-外骨骼耦合正向动力学仿真框架,对系统在平地和上坡(5.71°,10%)两种场景以0.8 m/s步速行走的动力学进行了比较研究.通过给定模型中的神经肌肉反射参数,综合考虑系统运动状态、肌肉收缩动力学、肌骨运动学、肌肉代谢、不同环境下的地反力和人机交互力等因素,并采用协方差矩阵自适应进化策略进行分阶段多目标优化,可以在不需要实验数据输入的条件下,高效完成全系统的动力学仿真.计算结果表明,所提仿真框架不仅能够生成包括肌肉激活、肌肉力、关节力矩和关节角度在内的完整生理学和生物力学信号,还能够反映和解释不同肌肉的激活模式随步行环境的适应性变化.此外还在与仿真情况一致的步行条件下进行了人体穿戴外骨骼行走测试,并进一步将肌肉激活数据与测得的肌电信号对比.结果表明,仿真与实验的肌肉激活结果保持了较高的相关性,并呈现出相对于地形坡度基本一致的变化规律;在上坡行走时,腘绳肌、臀大肌、髂腰肌和比目鱼肌的肌肉激活程度的RMS值均显著高于平地行走的情况,而股直肌与胫骨前肌的肌肉激活程度的RMS值则有所下降.上述结果表明,提出的动力学仿真框架可以在无实验数据输入的条件下有效且准确地捕捉在不同地形中行走时肌肉激活模式的定性差异.本研究为人体肌骨-外骨骼耦合系统在多场景下的行走仿真与性能评估提供了新的途径,也为外骨骼设计与控制策略的优化提供了理论参考和仿真分析方法.

关键词：下肢动力外骨骼动力学建模人机动力学肌肉骨骼模型肌肉激活

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Derivation of the multi-model generalized labeled multi-Bernoulli filter:a solution to multi-target hybrid systems

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Frontiers of Information Technology & Electronic Engineering 2021年第1期22卷 79-87页

作者： Weihua WU Yichao CAI hongbin jin Mao ZHENG Xun FENG Zewen GUAN Department of Early Warning Intelligence Air Force Early Warning AcademyWuhan 430019China

In this study,we extend traditional(single-target)hybrid systems to multi-target hybrid systems with a focus on the multi-maneuvering-target tracking system.This system consists of a continuous state,a discrete and switchable state,and a discrete,time-constant,and unique state.By defining a new generalized labeled multi-Bernoulli density,we prove that it is closed under the Chapman-Kolmogorov prediction and Bayes update for multi-target hybrid systems.In other words,we provide the exact derivation of a solution to this system,i.e.,the multi-model generalized labeled multi-Bemoulli filter,which has been developed without strict proof.

关键词： Multi-maneuvering-target tracking Multi-model Generalized labeled multi・Bemoulli filter Multi-target hybrid systems

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互联网工业时代企业PI战略——以小熊“萌家电”设计实践为例

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装饰 2024年第3期 106-111页

作者：蒋红斌赵妍金志强清华大学美术学院鲁迅美术学院

互联网经济要求企业更新PI设计战略。从实践角度,传统PI对产品固化的、单线的、外显化的设计规范不再适用,突显“集体意志”与“多部门协同创新”的动态PI为企业指明未来方向;从研究角度,传统PI多由设计部门制定,基于对现有产品的语义... 详细信息

互联网经济要求企业更新PI设计战略。从实践角度,传统PI对产品固化的、单线的、外显化的设计规范不再适用,突显“集体意志”与“多部门协同创新”的动态PI为企业指明未来方向;从研究角度,传统PI多由设计部门制定,基于对现有产品的语义分析和线形提取,并以量化结果构建缺乏自适应性和迭代能力的设计执行标准。动态PI的价值在于对企业有机的、全局的组织与管理能力,从宏观、中观和微观建立有原则、有法则、有要则的企业设计创新方针。以小熊电器为例,动态PI凭借对企业内外资源的有机整合,开启小熊“萌家电”时代,引领企业走出同质化、价格战的竞争“洼地”,最终为中国小家电企业量身定制多线并进、形神兼备、内外兼修的PI设计新方略。

关键词：动态PI设计企业设计战略设计组织管理家电设计

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展览馆钢结构施工技术研究

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工程设计与施工 2024年第2期6卷 62-64页

作者：靳红彬全国农业展览馆北京100125

随着建筑技术水平的提升,钢结构施工技术在建筑工程施工中发挥了越来越重要的作用,主要是因为钢结构施工工期短,安装方便,应用范围广,且承载力较高,抗震性能好,施工较为灵活。由于展览馆钢结构施工工程较为复杂,需要对钢结构施工关键点... 详细信息

随着建筑技术水平的提升,钢结构施工技术在建筑工程施工中发挥了越来越重要的作用,主要是因为钢结构施工工期短,安装方便,应用范围广,且承载力较高,抗震性能好,施工较为灵活。由于展览馆钢结构施工工程较为复杂,需要对钢结构施工关键点进行精准把控,如钢构节点对接、预制构件吊装施工、焊接施工等,保障整体施工质量的提升,保障钢结构可靠性、牢固性,减少安全事故的发生概率。论文主要对展览馆结构施工技术要点、控制策略等进行详细分析,旨在进一步提高展览馆钢结构施工质量,保障整体结构的安全可靠性运行,为展览馆功能作用的有效发挥奠定良好基础。

关键词：展览馆钢结构施工技术

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prpf4 is essential for cell survival and posterior lateral line primordium migration in zebrafish

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Journal of Genetics and Genomics 2018年第8期45卷 443-453页

作者： Yixia Wang Yanchao Han Pengfei Xu Shihui Ding Guangyuan Li hongbin jin Yaping Meng Anming Meng Shunji Jia State Key Laboratory of Membrane Biology Tsinghua-Peking Center for Life Sciences School of Life Sciences Tsinghua University

Prpf4 （pre-mRNA processing factor 4）, a key component of spliceosome, plays critical roles in pre-mRNA splicing and its mutations result in retinitis pigmentosa due to photoreceptor defects. In this study, we characterized a zebrafish prpf4t243 mutant harboring a Tol2 transposon-based gene trap cassette in the third intron of the prpf4 gene. Cells in the brain and spinal cord gradually undergo p53-dependent apoptosis after 28 hpf in prpf4t243 mutants, suggesting that a widespread function of prpf4 in neural cell survival. In addition, prpf4 is essential for survival of posterior lateral line primordial （pLLP） cells, prpf4 deficiency perturbs Fgf, Wnt/β-catenin and chemokine signaling pathways and impairs pLLP migration. RNA-Seq analysis suggests that prpf4 deficiency may impair spliceosome assembly, leading to compensatory upregulation of core spliceosomal genes and alteration of pre-mRNA splicing. Taken together, our studies uncover an essential role of prpf4 in pre-mRNA splicing, cell survival and pLLP migration.

关键词： prpf4 Splicing Apoptosisp LLP Zebrafish

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