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Triterpenoid saponin W3 from Anemone flaccida Suppresses Osteoclast Differentiation through Preventing the Activation of MAPKs/NF-kB/c-Fos/NFATc1 Cascades

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Triterpenoid saponin W3 from Anemone flaccida Suppresses Ost...

第十四届全国中药和天然药物学术研讨会

作者： Xiangying Kong Yue Yang Wenbin Wu hongye wan Xiaohui Su Shiwei Jia Na Lin China Academy of Chinese Medical Sciences

＜正＞Excessive bone resorption by osteoclasts within inflamed joints is the most specific hallmark of rheumatoid arthritis（RA）.And one of the ultimate goals in managing RA is to prevent or control joint ***,inhibit...

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Acupuncture and Moxibustion-like thermal stimulation activate cutaneous and systematic hypothalamic-pituitary-adrenal axis in rats

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Acupuncture and Moxibustion-like thermal stimulation activat...

新时代新思维新跨越新发展——2019中国针灸学会...

作者： Wei He Ying Li XiaoNing Zhang hongye wan QingQuan Yu XiangHong Jing Bing Zhu Institute of Acupuncture and Moxibustion China Academy of Chinese Medical Sciences

Background: The skin neuroendocrine system, named as skin-brain axis plays a role in regulation of local and global homeostasis, which can be activated by physical stimulation such as Ultraviolet B(UVB). As physical treating methods, acupuncture and moxibustion often induce comprehensive responses of the body function, which assists the body in returning to homeostasis. In the present study, we investigated the response of cutaneous and systematic hypothalamic-pituitary-adrenal(HPA) axis to electroacupuncture(EA) and moxibustion like thermal stimulation. Methods: Male Sprague Dawley rats were divided into two groups: ST 36 acupoint group and LI 4 acupoint group. Rats in each group were then divided into four subgroups: Control group, Electroacupuncutre(EA) group, 37°C stimulation group,43.5°C stimulation group. Bilateral ST 36 or LI 4 acupoints were chosen as the location for EAor thermal stimulation respectively. Electroacupuncture and moxibustion-like 43.5°C thermal stimulation were delivered at bilateral ST 36 acupoints or LI 4 *** techniques were used to observe cutaneous neurohormone expression of corticotropin-releasing factor(CRF), adrenocorticotropic hormone(ACTH), and glucocorticoid receptor(GR) at the acupoints at the acupoints. Concentrations of plasma and cutaneous CRH, ACTH, Corticosterone(CORT) were determined by enzyme linked immunosorbent assay or enzyme immunoassay. Results: Immunofluorescent staining of cutaneous CRF, ACTH and GR at the acupoints showed that CRF mainly expressed in cutaneous keratinocytes, hair bulb keratinocytes, ACTH mainly expressed in cutaneous hair bulb keratinocytes(Fig. 3). GR mainly expressed in keratinocytes and hair follicles(Fig. 4).Cutaneous levels of CRF, ACTH and CORT increased(P<0.01, P<0.05, P<0.01 vs Ctrl) after EA at the ST 36 acupoints, and cutaneous levels of CORT also increased(P<0.01, vs Ctrl or37°C) after 43.5 °C thermal stimulation at the ST 36 acupoints. Cutaneous levels of CORT

关键词： Acupuncture Moxibustion hypothalamic-pituitary-adrenal skin-brain axis

The Neuroprotective Effect of Buyang Huanwu Decoction on Rat Ischemic/Reperfusion Brain Damage by Promoting Migration of Neural Stem Cells

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The Neuroprotective Effect of Buyang Huanwu Decoction on Rat...

第十四届全国中药和天然药物学术研讨会

作者： Xiangying Kong Xiaohui Su Jia Zhu Jianzhu wang hongye wan Li Li Na Lin Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences wangJing Hospital of China Academy of Chinese Medical Sciences

Buyang Huanwu Decoction(BYHWD)is a classic formula widely used for treating stroke-induced disability,the highest morbidity of neurological disorders in ***,the mechanism of its neuroprotection has not been fully *** reports indicated that it may promote growth and differentiation of neural stem cells(NSCs).The present

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Wu-tou Decotion Alleviates CFA-induced Inflammatory Pain in Mice:Roles of TRPV1,TRPA1 and TRPM8 ion Channels

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Wu-tou Decotion Alleviates CFA-induced Inflammatory Pain in ...

第十四届全国中药和天然药物学术研讨会

作者： Chao wang Chunfang Liu Ying Xu hongye wan Yue Yang Danhua wang Danni Sun Na Lin China Academy of Chinese Medical Sciences

＜正＞Wu-tou decoction（WTD）,a traditional Chinese medicine（TCM）formula,which has been used for more than a thousand years to treat rheumatic arthritis,rheumatoid arthritis,pain of ***,there is no report about its antinociceptive ***,In a CFA-induced pain modle in mice,we explored the knowledge on the antinociceptive properties of WTD and showed that

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Anti-angiogenic effect of triptolide in rheumatoid arthritis by targeting angiogenic cascade

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Anti-angiogenic effect of triptolide in rheumatoid arthritis...

第十四届全国中药和天然药物学术研讨会

作者： Xiangying KONG Yanqiong ZHANG Chunfang LJU Wei GUO Xiangbin LI Xiaohui SU hongye wan Yanqun SUN Na LIN Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences

Rheumatoid arthritis(RA) is characterized by a pre-vascular seriously inflammatory phase,followed by a vascular phase with high increase in vessel *** angiogenesis has been considered as an essential event in perpetuating inflammatory and immune responses,as well as supporting pannus growth and development of RA,inhibition of angiogenesis has been proposed as a novel therapeutic strategy for ***,a diterpenoid triepoxide from Tripterygium wilfardii Hook F,has been extensively used in treatment of RA *** also acts as a small molecule inhibitor of tumor angiogenesis in several cancer ***,it is unclear whether triptolide possesses an anti-angiogenic effect in *** address this problem,we firstly constructed collagen-induced arthritis(CIA)model using DA rats by the injection of bovine type II ***,CIA rats were treated with triptolide(11-45 ug/kg/day) starting on the day 1 after first *** arthritis scores(P<0.05) and the arthritis incidence(P<0.05) of inflamed joints were both significantly decreased in triptolide-treated CIA rats compared to vehicle-treated CIA *** interestingly,doses of 115ug/kg triptolide could markedly reduce the capillaries,small,medium and large vessel density in synovial membrane tissues of inflamed joints(all P<0.05).Moreover,triptolide inhibited matrigel-induced cell adhesion of HFLS-RA and *** also disrupted tube formation of HUVEC on matrigel and suppressed the VEGF-induced chemotactic migration of HFLS-RA and HUVEC,***,triptolide significantly reduced the expression of angiogenic activators including TNF-α,IL-17,VEGF,VEGFR,Ang-1,Ang-2 and Tie2,as well as suppressed the IL1-β-induced phosphorylated of ERK,p38 and JNK at protein *** conclusion,our data suggest for the first time that triptolide may possess anti-angiogenic effect in RA both in vivo and in vitro assay systems by downregulating angiogenic activators and inhibiting the activation of

关键词： triptolide rheumatoid arthritis angiogenesis angiogenic activator mitogen-activated protein kinase

弗氏完全佐剂肌肉注射诱发炎性痛敏及免疫应答的转录组测序分析

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中国比较医学杂志 2022年第6期32卷 31-40页

作者：石惊涛张晓宁曹婉萦何伟刘一寒王睿宿杨帅万红叶曲正阳景向红王晓宇中国中医科学院针灸研究所北京100700 北京理工大学北京100081

目的观察弗氏完全佐剂(complete Freund’s adjuvant,CFA)肌肉注射后1~15 d的炎性反应,明确免疫应答反应的发生,通过分析转录组学的差异,为肌肉炎性反应及其诱发的痛觉敏化研究提供重要的参考。方法90只Wistar大鼠随机分为正常组和CFA... 详细信息

目的观察弗氏完全佐剂(complete Freund’s adjuvant,CFA)肌肉注射后1~15 d的炎性反应,明确免疫应答反应的发生,通过分析转录组学的差异,为肌肉炎性反应及其诱发的痛觉敏化研究提供重要的参考。方法90只Wistar大鼠随机分为正常组和CFA注射后第1天(MD1)、第3天(MD3)、第5天(MD5)、第7天(MD7)、第9天(MD9)、第11天(MD11)、第13天(MD13)和第15天(MD15)模型组。在模型组大鼠左侧股二头肌肌腹注入200μL的CFA,观察各组大鼠左侧足底的机械痛阈值和热痛阈值变化;采用电化学发光法检测各组大鼠肌肉组织和外周血中炎性因子的含量;使用HE染色对正常组、MD3和MD13组大鼠注射侧局部肌肉组织进行形态学观察;采用基因组学的方法,分析这两组大鼠差异基因的表达。结果(1)股二头肌肌肉注射CFA可诱发同侧足底持续的机械痛敏和热痛敏。(2)CFA肌肉注射后24 h后,外周血和局部组织中IL-6、IL-1β和TNF-α的含量明显升高,局部组织中促炎因子含量在注射后第13天再次明显升高。(3)在CFA注射后第3天局部组织以中性粒细胞浸润为主,在CFA注射后第13天则以单核细胞浸润为主。(4)与MD3组相比,MD13组大鼠局部组织中与免疫反应相关的差异表达基因上调,其中涉及到Th细胞的分化、细胞因子受体相互作用等与适应性免疫相关的生物学通路。(5)CFA注射后局部组织中痛敏相关基因的表达水平升高,包括Ccl3、Cxcl1、Cxcl2、Cxcl9、Cxcl10等基因。结论CFA的注射可有效刺激固有免疫和适应性免疫的发生,诱导炎性痛敏,其中不同趋化因子家族基因在不同的免疫应答中发挥了重要的作用。

关键词：弗氏完全佐剂肌肉炎性痛细胞因子固有免疫反应适应性免疫反应转录组测序

电针对乳腺癌小鼠抑瘤作用的时间窗及其免疫学机制的初步观察

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世界中医药 2022年第21期17卷 3039-3044页

作者：万红叶张知云张晓宁于清泉孙小月何伟王晓宇阚宇景向红中国中医科学院针灸研究所北京100700 福建中医药大学针灸学院福州350122

目的:观察肿瘤不同发展阶段电针干预对乳腺癌荷瘤小鼠肿瘤生长速度的影响,明确电针“足三里”抑瘤的最佳干预时间窗,并初步探讨电针抑瘤的免疫学机制。方法:BALB/c小鼠随机分为正常组、模型组、阳性药组、电针1组、电针2组、电针3组和电... 详细信息

目的:观察肿瘤不同发展阶段电针干预对乳腺癌荷瘤小鼠肿瘤生长速度的影响,明确电针“足三里”抑瘤的最佳干预时间窗,并初步探讨电针抑瘤的免疫学机制。方法:BALB/c小鼠随机分为正常组、模型组、阳性药组、电针1组、电针2组、电针3组和电针4组。原位接种4T1-luc细胞制备乳腺癌小鼠模型。对各电针组小鼠双侧“足三里”行电针干预(0.1 mA,2/15 Hz,30 min),阳性药组腹腔注射吉西他滨。选取正常组、模型组、电针3组、电针4组和阳性药组进行重复验证实验。采用游标卡尺和活体成像系统评估肿瘤尺寸;流式细胞术检测外周血抗肿瘤免疫细胞CD8^(+)T和自然杀伤细胞比例。结果:第16天、19天和22天,阳性药组、电针4组和电针3组小鼠肿瘤尺寸较模型组明显减小(P<0.05或P<0.01),且持续至观察结束。电针4组和阳性药组小鼠肿瘤重量较模型组明显减轻(P<0.05或P<0.01)。重复验证实验表明电针4组较电针3组抑瘤作用强,但差异无统计学意义。且电针3组和电针4组均可提高荷瘤小鼠外周血中CD8^(+)T和自然杀伤细胞比例,以电针4组作用更加明显。结论:成瘤后电针干预的抑瘤效果最好,且电针“足三里”可能通过提高抗肿瘤免疫应答减缓乳腺癌荷瘤小鼠肿瘤的生长速度。

关键词：电针足三里乳腺癌时间窗 CD8+T细胞自然杀伤细胞抗肿瘤免疫

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基于生态系统服务视角的云南生态茶产业内涵、实践与路径

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热带农业科学 2024年第3期44卷 109-114页

作者：伏成秀张庆付利波袁媛万思琦朱红业云南省农业科学院农业环境资源研究所云南昆明650205 云南省农业科学院农业经济与信息研究所云南昆明650205

生态茶产业是我国生态农业体系重要组成部分,是云南省茶叶高质量发展和茶叶强省发展的目标和必然选择。基于生态系统服务的视角,云南省生态茶产业具有供给生态优质茶产品、发挥高值生态服务效益、传承弘扬民族茶文化和支撑产业兴旺农民... 详细信息

生态茶产业是我国生态农业体系重要组成部分,是云南省茶叶高质量发展和茶叶强省发展的目标和必然选择。基于生态系统服务的视角,云南省生态茶产业具有供给生态优质茶产品、发挥高值生态服务效益、传承弘扬民族茶文化和支撑产业兴旺农民增收的生态农业内涵特征。梳理和剖析云南省茶产业生态系统服务功能价值的实践与挑战,从构建现代生态茶叶生产体系、系统推进生态茶园建设、打好民族茶文化牌和高质量发展生态茶产业方面,为云南生态茶产业高质量发展和多元生态系统服务价值实现提供思路和方法。

关键词：生态茶产业生态系统服务茶叶强省云南

电针对结直肠扩张刺激模型大鼠内脏伤害性痛反应的影响

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中医杂志 2019年第10期60卷 869-875页

作者：胡嘉同王晓宇何伟宿杨帅张晓宁万红叶李志刚北京中医药大学北京市100029 中国中医科学院针灸研究所

目的探讨不同强度、不同穴位电针刺激对结直肠扩张刺激引起的大鼠内脏伤害性痛反应的影响及其可能机制。方法 SD大鼠54只,分别进行电生理实验(30只)和形态学实验(24只)。电生理实验中先选出6只大鼠用于结直肠扩张(CRD)刺激诱导腹直肌肌... 详细信息

目的探讨不同强度、不同穴位电针刺激对结直肠扩张刺激引起的大鼠内脏伤害性痛反应的影响及其可能机制。方法 SD大鼠54只,分别进行电生理实验(30只)和形态学实验(24只)。电生理实验中先选出6只大鼠用于结直肠扩张(CRD)刺激诱导腹直肌肌电发生阈值观察,其余大鼠随机分为足三里组、天枢组和曲池组,每组8只,麻醉状态下给予CRD刺激造成大鼠内脏伤害性疼痛,采用1 mA和3 mA电流强度电针刺激大鼠"足三里""天枢""曲池",持续时间140 s,记录其对腹直肌肌电活动的曲线下面积、频率、振幅。形态学实验大鼠随机分为正常组、模型组、1 mA足三里组、1 mA天枢组、1 mA曲池组、3 mA足三里组、3 mA天枢组、3 mA曲池组,每组3只,电针刺激持续时间30 min,采用免疫组化方法观察不同强度不同穴位电针刺激对CRD刺激造成孤束核c-fos阳性神经元表达的影响。结果 60 mmHg的CRD可诱发大鼠内脏痛,表现为腹直肌肌电的曲线下面积、频率和振幅明显增加,与CRD前相比差异有统计学意义(P <0. 01),故将其选为CRD刺激值。1 mA电针"天枢"可以显著改善伤害性刺激引起的腹直肌肌电的发放(P <0. 05),c-fos阳性神经元在延髓孤束核表达的数量和密度均明显减少(P <0. 05)。而1 mA电针"足三里"和"曲池",对CRD引起的腹直肌肌电发放没有显著改善。3 mA电针"足三里"和"天枢",均可以明显抑制CRD引起的腹直肌肌电的发放(P <0. 01),并减少CRD造成c-fos在孤束核的表达增加(P <0. 05)。结论电针"天枢"和"足三里"均可以显著改善CRD引起的伤害性痛反应,减少c-fos阳性神经元在大鼠延髓孤束核的表达,但同节段"天枢"抑制内脏痛所需的刺激量较小。

关键词：内脏痛刺激强度电针孤束核 c-fos