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Against all odds:The road to success in the development of human immune reconstitution mice

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Animal Models and Experimental Medicine 2024年第4期7卷 460-470页

作者： Yixiao Bin Jing Ren Haowei Zhang Tianjiao Zhang Peijuan Liu Zhiqian Xin Haijiao Yang zhuan Feng Zhinan chen Hai Zhang School of Basic Medical Sciences Shaanxi University of Chinese MedicineXianyangChina Department of Cell Biology National Translational Science Center for Molecular MedicineFourth Military Medical UniversityXi'anChina State Key Laboratory of New Targets Discovery and Drug Development for Major Diseases Fourth Military Medical UniversityXi'anChina Department of Occupational&Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment School of Public HealthFourth Military Medical UniversityXi'anChina

The mouse genome has a high degree of homology with the human genome,and its physiological,biochemical,and developmental regulation mechanisms are similar to those of humans;therefore,mice are widely used as experimental ***,it is undeniable that interspecies differences between humans and mice can lead to experimental *** differences in the immune system have become an impor-tant factor limiting current immunological *** application of immunodefi-cient mice provides a possible solution to these *** transplanting human immune cells or tissues,such as peripheral blood mononuclear cells or hematopoietic stem cells,into immunodeficient mice,a human immune system can be reconstituted in the mouse body,and the engrafted immune cells can elicit human-specific immune *** have been actively exploring the development and differen-tiation conditions of host recipient animals and grafts in order to achieve better im-mune *** genetic engineering methods,immunodeficient mice can be further modified to provide a favorable developmental and differentiation microenvironment for the *** initially only being able to reconstruct single T lymphocyte lineages,it is now possible to reconstruct lymphoid and myeloid cells,providing important research tools for immunology-related *** this review,we compare the differences in immune systems of humans and mice,describe the devel-opment history of human immune reconstitution from the perspectives of immuno-deficient mice and grafts,and discuss the latest advances in enhancing the efficiency of human immune cell reconstitution,aiming to provide important references for im-munological related researches.

关键词： hematopoietic stem cell human immune reconstitution immune response immunodeficient mice peripheral blood mononuclear cell transplantation

PMS777 attenuates LPS-induced acute neuroinflammation

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PMS777 attenuates LPS-induced acute neuroinflammation

Endoplasmic reticulum-targeted delivery of celastrol and PD-L1 siRNA for reinforcing immunogenic cell death and potentiating cancer immunotherapy

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Jin-Jia HU,Juan LI,Zhang WEI,Bi-Yun SHAO,Ding-Wen LONG,hong-zhuan chen~* Department of Anatomy and Pharmacology,Shanghai Jiaotong University,Shanghai 200025,China

The proinflammagen lipopolysaccharide(LPS) injected into the forth ventricle could induce acute neuroinflammation in the whole brain of *** current study determined whether the new compound PMS777—a platelet-activating factor receptor(PAFR) antagonist and acetylcholinesterase(AChE) inhibitor could provide significant neuroprotection against the cytotoxic effects of LPS-induced *** LPS infusions impared recognition, decreased the number of AChE positive cells,and increased the number of OX-42 immunoreactive microglia and GFAP immunoreactive cells in hippocampus,cortex and basal *** LPS infusions also impaired organelle of protein *** administration of PMS777(i.P.) significantly protected against the recognition impairment,attenuated the cytotoxic effects of the acute inflammatory processes upon cholinergic cells,microglia,astrocytes and ultrastructure of hippocampal *** we report that the cytotoxic effects of acute neuroinflammation may involve PAF releasing and loss of cholinergic neurons,leading to the neuronal dysfunction and spatial memory *** dual PAFR AChE inhibitors could provide excellent neuroprotection from the cytotoxic effects induced by LPS.

关键词： neuroinflammation spatial memory acetylcholinesterase platelet-activating factor ultrasturcture

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3643-3660页

作者： Jie Wang Zilong Zhang Yan Zhuo zhuan Zhang Rongrong chen Li Liang Xiaohe Jiang Di Nie Chang Liu Zhiwen Zou Xiang Li Jiaxin Li Bingqi Wang Rui Wang Yong Gan Miaorong Yu School of Pharmacy Shanghai University of Traditional Chinese MedicineShanghai 201203China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Jiangxi Medical College Nanchang UniversityNanchang 330006China School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China University of Chinese Academy of Sciences Beijing 100049China NMPA Key Laboratory for Quality Research and Evaluation of Pharmaceutical Excipients National Institutes for Food and Drug ControlBeijing 100050China

The prospect of employing chemoimmunotherapy targeted towards the endoplasmic reticulum(ER)presents an opportunity to amplify the synergistic effects of chemotherapy and *** this study,we initially validated celastrol(CEL)as an inducer of immunogenic cell death(ICD)by promoting ER stress and autophagy in colorectal cancer(CRC)***,an ER-targeted strategy was posited,involving the codelivery of CEL with PD-L1 small interfering RNAs(siRNA)using KDEL peptide-modified exosomes derived from milk(KME),to enhance chemoimmunotherapy *** findings demonstrate the efficient transportation of KME to the ER via the Golgi-to-ER *** to their non-targeting counterparts,KME exhibited a significant augmentation of the CEL-induced ICD ***,it facilitated the release of danger signaling molecules(DAMPs),thereby stimulating the antigen-presenting function of dendritic cells and promoting the infiltration of T cells into the ***,the ER-targeted delivery of PD-L1 siRNA resulted in the downregulation of both intracellular and membrane PD-L1 protein expression,consequently fostering the proliferation and activity of CD8^(+)T ***,the ER-targeted formulation exhibited enhanced anti-tumor efficacy and provoked anti-tumor immune responses against orthotopic colorectal tumors in ***,a robust ER-targeted delivery strategy provides an encouraging approach for achieving potent cancer chemoimmunotherapy.

关键词： Chemoimmunotherapy Targeted drug delivery Endoplasmic reticulum Endoplasmic reticulum stress Immunogenic cell death siRNA Chemoimmunotherapy Targeted drug delivery Endoplasmic reticulum Endoplasmic reticulum stress Immunogenic cell death siRNA

Expert consensus on irrigation and intracanal medication in root canal therapy

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International Journal of Oral Science 2024年第1期16卷 26-35页

作者： Xiaoying Zou Xin Zheng Yuhong Liang chengfei Zhang Bing Fan Jingping Liang Junqi Ling zhuan Bian Qing Yu Benxiang Hou Zhi chen Xi Wei Lihong Qiu Wenxia chen Wenxi He Xin Xu Liuyan Meng chen Zhang Liming chen Shuli Deng Yayan Lei Xiaoli Xie Xiaoyan Wang Jinhua Yu Jin Zhao Song Shen Xuedong Zhou Lin Yue Department of Cariology and Endodontology Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental MaterialsBeijingChina Center of Stomatology Peking University HospitalBeijingChina State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Emergency Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental MaterialsBeijingChina Restorative Dental Sciences EndodonticsFaculty of DentistryThe University of Hong KongPok Fu LamHong Kong SARChina State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration Key Laboratory of Oral Biomedicine Ministry of EducationHubei Key Laboratory of StomatologySchool&Hospital of StomatologyWuhan UniversityWuhanChina Department of Endodontics Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Clinical Research Center for Oral DiseasesNational Center for StomatologyShanghai Key Laboratory of StomatologyShanghaiChina Department of Operative Dentistry and Endodontics Hospital of StomatologyGuanghua School of StomatologySun Yat-Sen University&Guangdong Provincial Key Laboratory of StomatologyGuangzhouChina State Key Laboratory of Oral&Maxillofacial Reconstruction and R

Chemical cleaning and disinfection are crucial steps for eliminating infection in root canal treatment. However, irrigant selection or irrigation procedures are far from clear. The vapor lock effect in the apical region has yet to be solved, impeding irrigation efficacy and resulting in residual infections and compromised treatment outcomes.

关键词： treatment irrigation Expert

Propofol attenuates oxidative stress-induced PC12 cell injury via p38 MAP kinase dependent pathway

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Acta Pharmacologica Sinica 2007年第8期28卷 1123-1128页

作者： Xing-jun WU~2 Yong-jun ZHENG~3 Yong-yao CUI~2 Liang ZHU~2 Yang LU~(2,4) hong-zhuan chen~(2,4) ~2Department of Pharmacy Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China ~3Department of Anesthesiology,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200001,China Department of Pharmacy Shanghai Jiao Tong University School ofMedicineShanghai 200025China Department of Anesthesiology Renji HospitalShanghai Jiao TongUniversity School of MedicineShanghai 200001China

Aim:To investigate the neuroprotective effect of propofol and its intracellularmechanism on neurons in ***:Cell viability was determined with 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyl tetrazolium bromide *** celldeath was determined by Hoechst 33258 staining and a fluorescence-activatedcell *** caspase-3 activity was measured by fluorometric ***-activated protein（MAP）kinase phosphorylation was detected with ***:The pretreatment of rat pheochromocytoma cell line PC 12 withpropofol（1-10μmol/L）resulted in a significant recovery from hydrogen peroxide（H2O2）-induced cell death and the inhibition of H2O2induced caspase-3 activationand PC12 cell *** inhibited the H2O2-induced p38 MAP kinase,but not c-Jun N-terminal kinase or extracellular signal-regulated kinase 1 and ***:Propofol might attenuate H2O2-induced PC12 cell deaththrough the inhibition of signaling pathways mediated by the p38 MAP kinase.

关键词： propofol oxidative stress PC12 cells apoptosis p38 MAP kinase

Expert consensus on difficulty assessment of endodontic therapy

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International Journal of Oral Science 2024年第1期16卷 15-25页

作者： Dingming Huang Xiaoyan Wang Jingping Liang Junqi Ling zhuan Bian Qing Yu Benxiang Hou Xinmei chen Jiyao Li Ling Ye Lei cheng Xin Xu Tao Hu hongkun Wu Bin Guo Qin Su Zhi chen Lihong Qiu Wenxia chen Xi Wei Zhengwei Huang Jinhua Yu Zhengmei Lin Qi Zhang Deqin Yang Jin Zhao Shuang Pan Jian Yang Jiayuan Wu Yihuai Pan Xiaoli Xie Shuli Deng Xiaojing Huang Lan Zhang Lin Yue Xuedong Zhou State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Operative Dentistry and Endodontics West China Hospital of StomatologySichuan UniversityChengduChina Department of Cariology and Endodontology Peking University School and Hospital of Stomatology&National Center of Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of Stomatology&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental MaterialsBeijingChina Department of Endodontics Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Clinical Research Center for Oral DiseasesNational Center for StomatologyShanghai Key Laboratory of StomatologyShanghaiChina Department of Operative Dentistry and Endodontics Hospital of StomatologyGuanghuaSchool of StomatologySun Yat-Sen University&Guangdong Provincial Key Laboratory of StomatologyGuangzhouChina The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School and Hospital of StomatologyWuhan UniversityWuhanChina Department of Operative Dentistry&Endodontics School of StomatologyThe Fourth Military Medical UniversityXi’anChina Department of Endodontics Beijing Stomatological HospitalSchool of StomatologyCapital Medical UniversityBeijingChina State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Preventive Dentistry West China Hospital of StomatologySichuan UniversityChengduChina State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Geriatric dentistry West China Hospital of Stomatology

Endodontic diseases are a kind of chronic infectious oral disease. Common endodontic treatment concepts are based on the removal of inflamed or necrotic pulp tissue and the replacement by gutta-percha. However, it is very essential for endodontic treatment to debride the root canal system and prevent the root canal system from bacterial reinfection after root canal therapy(RCT). Recent research, encompassing bacterial etiology and advanced imaging techniques, contributes to our understanding of the root canal system’s anatomy intricacies and the technique sensitivity of RCT. Success in RCT hinges on factors like patients, infection severity, root canal anatomy, and treatment techniques. Therefore, improving disease management is a key issue to combat endodontic diseases and cure periapical lesions. The clinical difficulty assessment system of RCT is established based on patient conditions, tooth conditions, root canal configuration, and root canal needing retreatment, and emphasizes pre-treatment risk assessment for optimal outcomes. The findings suggest that the presence of risk factors may correlate with the challenge of achieving the high standard required for RCT. These insights contribute not only to improve education but also aid practitioners in treatment planning and referral decision-making within the field of endodontics.

关键词： anatomy difficulty treatment

The PARF Antagonist AChE Inhibitor PMS777 Attenuates LPS-Induced Acute Neuroinflammation

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Neuroscience & Medicine（神经系统科学与医药） 2011年第4期

作者： Jin-Jia Hu Juan Li Zhang Wei hong-Yu Gu Bi-Yun Shao Ding Wen Long hong-zhuan chen

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新矿物(U-Th)/He年代学的研究进展

地球科学进展 2024年第4期39卷 357-373页

作者：陈玉柳丁汝鑫孙转卢胜城广东省地球动力作用与地质灾害重点实验室中山大学地球科学与工程学院广东珠海519082 广东省地质过程与矿产资源探查重点实验室广东珠海519082 中国石油辽河油田勘探开发研究院辽宁盘锦124010

自20世纪末(U-Th)/He定年技术诞生以来,(U-Th)/He定年在地质学等领域发挥着越来越重要的作用,尤其是磷灰石及锆石的(U-Th)/He定年技术得到了广泛应用,然而这两种矿物在自然界中的分布相对有限,极大地限制了(U-Th)/He年代学的发展。近年... 详细信息

自20世纪末(U-Th)/He定年技术诞生以来,(U-Th)/He定年在地质学等领域发挥着越来越重要的作用,尤其是磷灰石及锆石的(U-Th)/He定年技术得到了广泛应用,然而这两种矿物在自然界中的分布相对有限,极大地限制了(U-Th)/He年代学的发展。近年来,随着He扩散动力学研究的不断深入和分析测试技术的提高,其他矿物的(U-Th)/He定年技术也得到了长足进步,理论与技术方法也日臻完善,为(U-Th)/He年代学开辟了新的应用领域。此外,不同类型的矿物记录了不同的地质信息,通过结合多种矿物的(U-Th)/He定年数据,将增进对地质过程的理解。基于(U-Th)/He定年新理论和新技术的发展,总结了赤铁矿、针铁矿、磁铁矿、碳酸盐矿物、牙形石、萤石、钙钛矿、尖晶石、金红石以及石榴子石等矿物的(U-Th)/He定年,重点介绍了相对成熟的赤铁矿、针铁矿、磁铁矿、碳酸盐矿物以及牙形石的(U-Th)/He定年的研究进展。目前,这些新矿物的(U-Th)/He定年在矿床学、沉积学、构造地质学、地球动力学和环境科学等领域得到了初步应用,尤其在约束成矿时代、古环境古气候重建、洋壳蚀变和俯冲剥露过程、热液活动、断裂变形以及古地震研究等方面将发挥关键作用。然而,这些新矿物的(U-Th)/He年代学仍存在不少问题,限制了这些方法的广泛推广与应用,比如多扩散域行为、辐射损伤和化学成分对He扩散的影响、加热脱气过程中母体同位素的挥发以及(U-Th)/He体系的开放行为等,这些因素将导致(U-Th)/He年龄数据表现出较大的分散性。因此,未来需要进一步研究He在这些矿物中的扩散行为,改善实验方法并提高仪器测试精度,以确保(U-Th)/He定年数据的准确性,为理解地质过程提供更加可靠的时间框架。

关键词： (U-Th)/He定年赤铁矿针铁矿磁铁矿碳酸盐矿物牙形石

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铜藻多酚的体外抗炎和降血糖活性

食品科学 2024年第3期45卷 68-75页

作者：何袅袅李孟昱蔡树芸施丽君陈伟珠陈晖洪专张怡张怡评自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心福建厦门361005 福建农林大学食品科学学院福建福州350001 福建省海岛资源生态监测与保护利用重点实验室福建厦门361005

利用大孔吸附树脂纯化铜藻多酚粗提液,并研究纯化后铜藻多酚的体外抗炎和降血糖活性。利用脂多糖诱导RAW 264.7巨噬细胞建立体外炎症模型,结果发现,铜藻多酚降低炎症介质NO含量的最佳质量浓度为40μg/m L。与模型组相比,30μg/mL的铜藻... 详细信息

利用大孔吸附树脂纯化铜藻多酚粗提液,并研究纯化后铜藻多酚的体外抗炎和降血糖活性。利用脂多糖诱导RAW 264.7巨噬细胞建立体外炎症模型,结果发现,铜藻多酚降低炎症介质NO含量的最佳质量浓度为40μg/m L。与模型组相比,30μg/mL的铜藻多酚溶液可使白细胞介素(interleukin,IL)-6、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和IL-1β 3种炎症因子的mRNA水平显著下调,对炎症因子TNF-α的蛋白表达水平有显著抑制作用,但是对炎症因子IL-1β蛋白表达水平抑制作用不显著,铜藻多酚对炎症因子的抑制作用与浓度呈正相关;铜藻多酚对α-葡萄糖苷酶的半数抑制浓度为5.96μg/mL,根据双倒数曲线发现铜藻多酚对α-葡萄糖苷酶的竞争性抑制常数Kic值为0.06μg/mL,非竞争性抑制常数Kiu值为6.68μg/mL,说明铜藻多酚对α-葡萄糖苷酶的抑制类型是非竞争性大于竞争性的混合可逆性抑制。以上结果表明铜藻多酚具有较好的抗炎和降血糖活性,可以作为一种天然的原料,应用于食品、保健品和化妆品等领域。

关键词：铜藻多酚抗炎活性 α-葡萄糖苷酶活性抑制动力学