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World Journal of Clinical Cases 2025年第3期13卷 42-49页

作者： Na Li yue-yue Ren Ying Tang Qi Yang Tian-Tian Meng Song Li Jing Zhang Stomatological Hospital and College Anhui Medical UniversityHefei 230032Anhui ProvinceChina Department of Endodontics The Affiliated Stomatological Hospital of Xuzhou Medical UniversityXuzhou 221000Jiangsu ProvinceChina

BACKGROUND Complicated crown–root fracture (CRF) involves severe injury to the crown, root,and pulp, and may be accompanied by multiple root fractures. The loss of a toothhas lifelong consequences for children and teenagers, but the maintenance of pulphealth and the calcific healing of multiple root fractures are rarely reported in *** SUMMARY This case reports healing of a permanent tooth with complicated crown–root andadditional root fractures, in which pulp health was maintained. A 10-year-old girlfell and fractured the root of her maxillary left central incisor at the cervical *** the coronal fragment was repositioned, the tooth was splinted until thetooth was no longer mobile, 2 years later. Eight years after treatment, the toothhas remained asymptomatic with vital pulp and localized gingival ***-beam computed tomography revealed not only calcified healing of the CRFbut also spontaneous healing in an additional undiagnosed root fracture. Thefracture line on the enamel could not be healed by hard tissue and formed agroove in the cervical crown. It was speculated that the groove was related to thelocalized gingival *** This case provides a clinical perspective of the treatment of a tooth with acomplicated CRF and an additional root fracture.

关键词： Complicated crown-root fracture Multiple root fracture Spontaneous healing Cone-beam computed tomography Long-term follow-up Case report

Experimental Studies on Sloshing Mitigation Using Dual Perforated Floating Plates in A Rectangular Tank

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China Ocean Engineering 2021年第2期35卷 301-307页

作者： yu yue-min College of Civil Engineering and Architecture Hainan UniversityHaikou 570228China State Key Laboratory of Marine Resource Utilization in South China Sea Hainan UniversityHaikou 570228China

The performance of dual perforated floating plates in a rectangular tank is investigated based on the model tests under different external excitations for different filling *** is found that dual perforated floating plates in the tank can remarkably mitigate violent resonant sloshing responses compared with the clean tank,especially when the external excitation frequency is in the vicinity of the first-order resonant ***,the parametric studies based on different filling rates and external excitation amplitudes are performed for the first-order resonant *** presence of dual perforated floating plates seldom shifts the sloshing natural ***,dual perforated floating plates change the sloshing modes from the standing-wave mode in the clean tank to the Utube mode,which can arise from the sloshing reduction to some extent.

关键词： suppressing sloshing experiment dual perforated floating plates the lowest natural frequency sloshing mode

Efficient photocatalytic reduction of CO2 to formate by a molecular noble metal-free system

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Science China Chemistry 2025年第1期 152-156页

作者： Lihua Zhang Lingjing Chen Huatian Shi yue Wei Gui Chen Tai-Chu Lau School of Environment and Civil Engineering Research Center for Eco-environmental Engineering Dongguan University of Technology Department of Chemistry and Institute of Molecular Functional Materials City University of Hong Kong

Reducing CO2to carbon-neutral solar fuels can not only alleviate the energy demand but also mitigate the greenhouse *** present,developing an efficient non-noble metal photo/electrocatalytic system for converting CO2to a specific product beyond CO is still a big ***,we report a new cobalt complex bearing 2,2′-bi-1,10-phenanthroline ligand that can photocatalytically reduce CO2to formate （HCOO-）.Turnover number （TON） for HCOO-up to 677 with 99%selectivity can be achieved using organic dyes purpurin as photosensitizer and 1,3-dimethyl-2-phenyl-2,3-dihydro-1H-benzo[d]imidazole （BIH） as sacrificial reductant in a CO2-saturated NMP/TEA （v/v,3:1） solution under visible light *** potential electrolysis at-1.41 V （***/Ag Cl） in the presence of 12 m M Et3NHBF4also produced HCOO-with Faradaic efficiency of 39%.Spectroscopic and electrochemical studies suggest that a[CoI-H]species is the key intermediate for CO2reduction.

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报废血液的初步分析

中国当代医药 2015年第17期22卷 4-7页

作者：王婧陈勇军朱明喻洪跃李长清中国医学科学院北京协和医学院输血研究所成都610052

目的初步了解国内报废血液的基本现状,为充分利用报废血资源提供参考和建议。方法首先,通过问卷发放、实地调研等形式对全国部分大型血站进行调研;其次,采用统计分析中定性与定量方法对数据进行整理与分析。结果血站报废血量占全年采血... 详细信息

目的初步了解国内报废血液的基本现状,为充分利用报废血资源提供参考和建议。方法首先,通过问卷发放、实地调研等形式对全国部分大型血站进行调研;其次,采用统计分析中定性与定量方法对数据进行整理与分析。结果血站报废血量占全年采血量的比例平均为5.4%;血液报废原因主要为丙氨酸氨基转移酶(ALT)不合格、脂血;约53.85%的血站对报废血液进行了少量利用,其余血站对其销毁处理。在调研的基础之上,构建了报废血液的监控流程图。结论得到的相关分析结论,对国内报废血液的总体情况有了一个大致的了解,为进一步合理、有效、充分地利用报废血液资源提供了一定的研究基础。

关键词：报废血液问卷调研报废原因报废处置

t(4;11)translocation in hyperdiploid de novo adult acute myeloid leukemia:A case report

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World Journal of Clinical Cases 2022年第32期10卷 11980-11986页

作者： Min-yu Zhang yue Zhao Ji-hong Zhang Hematology Laboratory Shengjing Hospital of China Medical UniversityShenyang 110022Liaoning ProvinceChina

BACKGROUND MLL gene rearrangement is a common genetic abnormality of acute myeloid leukemia(AML),which predicts poor prognosis and is important in clinical *** rearrangement involves many chromosomes,among which,t(4;11)translocation is rare in *** present case was t(4;11)AML,accompanied by a hyperdiploid *** cases have not been reported *** SUMMARY An adult male with self-reported symptoms of fatigue,febrility and hyperleukocytosis was diagnosed with AML by morphology and confirmed by immunophenotype ***,chromosomal and fluorescence in situ hybridization(FISH)analysis showed a hyperdiploid karyotype with t(4;11)translocation and MLL rearrangement,and a negative MLL–AF4 fusion gene *** patient died of respiratory and circulatory failure 5 days after *** t(4;11)AML with hyperdiploid karyotype has not been *** this case,t(4;11)was only detected by karyotype analysis and FISH,suggesting their importance in MLL rearrangement detection.

关键词： Acute myeloid leukemia MLL gene rearrangement Translocation t(4 11) Hyperdiploid FISH Case report

基于图论分析对轻度创伤性颅脑损伤后睡眠障碍患者的脑结构网络改变的研究

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临床放射学杂志 2025年第1期44卷 47-52页

作者：俞越杨禹李洋蔡玉姣王扬李月峰苏辉江苏大学医学院镇江212013 江苏大学附属人民医院镇江212001 扬州大学第三临床医学院 225600

目的探究轻度创伤性颅脑损伤患者脑结构网络的改变与未来睡眠障碍的关联。方法前瞻性搜集轻度创伤性颅脑损伤患者198例(mTBI组)及与之匹配的健康对照者60名(HC组)。以出院3个月后的匹兹堡睡眠质量指数量表(PSQI)为评估手段,将轻度创伤... 详细信息

目的探究轻度创伤性颅脑损伤患者脑结构网络的改变与未来睡眠障碍的关联。方法前瞻性搜集轻度创伤性颅脑损伤患者198例(mTBI组)及与之匹配的健康对照者60名(HC组)。以出院3个月后的匹兹堡睡眠质量指数量表(PSQI)为评估手段,将轻度创伤性颅脑损伤患者分为睡眠障碍组(mTBI-SD组)和无睡眠障碍组(mTBI-ND组)。利用方差分析比较3组受试者脑结构网络属性。构建Logistic回归模型探究脑结构网络属性与未来睡眠障碍发病风险的关联。采用Pearson相关分析探究脑结构网络属性与PSQI评分的相关性。结果3组受试者脑结构网络属性存在显著的组间差异。与mTBI-ND组相比,mTBI-SD组表现出较高的特征路径长度,较低的左前扣带回和旁扣带回节点效率。Logistic回归模型显示特征路径长度(OR特征路径长度=0.74,95%CI:0.63~0.94,P<0.001)、左前扣带和旁扣带回的节点效率(OR ACG.L=0.70,95%CI:0.65~0.80,P<0.001)可以显著预测mTBI-SD的发生,受试者工作特征曲线下面积分别为0.75、0.74。相关分析结果显示mTBI-SD组的特征路径长度(r=-0.41,P<0.01)、左前扣带和旁扣带回的节点效率(r=-0.50,P<0.01)与发病后3个月的PSQI评分相关。结论mTBI显著改变患者的脑结构网络连接属性,存在特征路径长度、左前扣带回和旁扣带回的节点效率改变的患者更容易在未来发生睡眠障碍。

关键词：轻度创伤性颅脑损伤睡眠障碍结构网络图论分析

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急性白血病p16基因的研究(英文)

中国实验血液学杂志 1998年第4期6卷 269-272页

作者：陈燕张红宇乐蓓蓓李慧玉同济医科大学附属协和医院血液病研究所武汉430022

为研究p16抑癌基因在急性白血病中的变化,用ABC法研究了61例急性白血病细胞表面p16抗原的表达和用多重PCR法研究了51例急性白血病p16基因的结构缺陷。结果发现白血病患者p16抗原的表达明显低于正常人(P0.05)。在30例ALL中仅发现4例p16... 详细信息

为研究p16抑癌基因在急性白血病中的变化,用ABC法研究了61例急性白血病细胞表面p16抗原的表达和用多重PCR法研究了51例急性白血病p16基因的结构缺陷。结果发现白血病患者p16抗原的表达明显低于正常人(P0.05)。在30例ALL中仅发现4例p16基因第二外显子纯合子缺失,在21例AML未发现pl6基因的结构变化,说明p16基因的表达缺陷是急性白血病发生和发展中的主要变化,而结构异常并不是其演变中的必需分子事件。

关键词：急性白血病 p16基因基因缺失

用于肉毒毒素活性测定的特异性绿色荧光融合蛋白的制备

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中国生物制品学杂志 2017年第9期30卷 901-905页

作者：吉元刚张国利李泽鸿岳玉环吴广谋田园刘雨玲李玉洁赵鑫付玉和王冬冬张培培侯天全徐艳玲马洪园军事医学科学院军事兽医研究所吉林长春130122 吉林农业大学生命科学学院吉林长春130118

目的制备含有7种血清型肉毒毒素切割位点(SNAP25-VAMP)的特异性绿色荧光融合蛋白,在***中诱导表达,纯化后用于肉毒毒素活性测定。方法根据SNARE蛋白复合物中SNAPE25和VAMP基因序列及各血清型肉毒毒素(Bo NTs)的切割位点,设计合成含有SNA... 详细信息

目的制备含有7种血清型肉毒毒素切割位点(SNAP25-VAMP)的特异性绿色荧光融合蛋白,在***中诱导表达,纯化后用于肉毒毒素活性测定。方法根据SNARE蛋白复合物中SNAPE25和VAMP基因序列及各血清型肉毒毒素(Bo NTs)的切割位点,设计合成含有SNAREs中突触小体(SNAP25)和突触小泡膜蛋白(VAMP)的Bam HⅠ-HIS6-SNAP62-Eco RⅠ-VAMP57C-TAA-HindⅢ(以下简称为SV)基因,连接至p ET-28a-GFP载体上,构建重组质粒p ET-28a-GFP-SV,转化感受态*** BL21(DE3),IPTG诱导表达后,经DEAE阴离子交换层析、Cu2+金属螯合层析、Q阴离子交换层析3步纯化,BCA法测定纯化产物的蛋白浓度,ELISA法检测B型肉毒毒素轻链蛋白(Bo NT/BL)活性。结果构建的质粒p ET-28a-GFP-SV经双酶切及测序鉴定证明构建正确,表达的GFP-SV融合蛋白相对分子量约40 000,主要以可溶形式存在,蛋白浓度为18.4μg/μl,纯度可达70%以上。利用该融合蛋白的荧光强弱变化可测定Bo NT/BL活性大小,同时也可测定其抗体中和活性大小。结论制备的含有SV的特异性绿色荧光融合蛋白在***中获得了高效表达,为后续各型肉毒毒素活性检测及抗体中和毒素活性检测奠定了基础。

关键词： SNAP25-VAMP 绿色荧光蛋白肉毒毒素活性血清型

The microbiota in spider mite feces potentially reflects intestinal bacterial communities in the host

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Insect Science 2020年第5期27卷 859-868页

作者： yu-Xi Zhu Zhang-Rong Song yue-Ling Song Dian-Shu Zhao Xiao-yue hong Department of Entomology Nanjing Agricultural UniversityNanjingChina

Microorganisms prov ide many physiological functions to herbivorous *** mites(genus Tetranychus)are important agricultural pests throughout the world;however,the composition of the spider mite microbial community,especially gut micro-biome,remains ***,we investigated the bacterial community in five spider mite species and their associated feces by deep sequencing of the 16S rRNA *** composi-tion of the bacterial community was significantly different among the five prevalent spider mite species,and some bacterial symbionts showed host-species ***,the abundance of the bacterial community in spider mite feces was significantly higher than that in the corresponding spider mite ***,Flavobacterium was detected in all samples,and represent a"core microbiome".Remarkably,the maternally inherited endosymbiont Wolbachia was detected in both spider mite and ***,these results offer insight into the complex community of symbionts in spider mites,and give a new direction for future studies.

关键词： bacterial community fces 16S rRNA spider mite Wolbachia

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稀土复合氧化物燃烧催化剂的性能及表征

稀土 2000年第5期21卷 13-15页

作者：胡瑞生王红宇沈岳年赵明辉内蒙古大学化学系内蒙古呼和浩特010021 内蒙古武警指挥学校内蒙古呼和浩特010062

浸渍法制备了稀土型燃烧催化剂 ,采用多晶 X射线衍射 (XRD)、程序升温还原 (TPR)、差热热重分析(DTA- TG)、X射线光电子能谱 (XPS)

关键词：稀土复合氧化物负载型燃烧催化剂结构性能