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Screening for a new Streptomyces strain capable of efficient keratin degradation

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Journal of Environmental Sciences 2007年第9期19卷 1125-1128页

作者： CHAO Ya-peng XIE Fu-hong yang Jing lu Jing-hua QIAN Shi-jun State Key Laboratories of Transducer Technology Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China.

Keratinous wastes could be degraded by some microorganisms in nature. Native human foot skin （NHFS） was used as sole nitrogen source to screen microorganisms with keratin-degrading capability. From approximately 200 strains, a strain of Streptomyces sp. strain No. 16 was found to possess the strongest keratinolytic activity, and the total activity in the culture was 110 KU/ml with specific activity of 2870 KU/mg protein （KU： keratinase unit）. Substrate specificity test indicated that the crude keratinase could degrade keratin azure, human hair, cock feathers and collagen. The optimal pH of the crude keratinase ranged from 7.5 to 10 and the temperature ranged from 40℃ to 55℃. Metal chelating agent ethylenediamine tetraacetic acid obviously stimulated the keratinolytic activity but suppressed the proteolytic activity. To our knowledge, this is the first report on specific induction of keratinases by NHFS from an actinomycete. Moreover, excellent characteristics of its crude keratinase may lead to the potential application in waste treatment and recovery, poultry and leather industry, medicine, and cosmetic development.

关键词： keratinolytic capability Streptomyces sp. native human foot skin (NHFS) keratinase substrate specificity

KRAS Exon 3 and PTEN Exon 7 Mutations in Small-cell lung Cancer

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Current Medical Science 2019年第3期39卷 379-384页

作者： Lei LEI Zhi-ming JIANG Cheng-hui LI hong-yang lu Zhejiang Key Laboratory of Diagnosis & Treatment Technology on Thoracic Oncology (lung and Esophagus) Zhejiang Cancer Hospital Hangzhou 310022China Department of Medical Oncology Zhejiang Cancer Hospital Hangzhou 310022China

Small cell lung cancer (SCLC) is recognized as one of the most aggressive and fatal malignant tumors. No significant improvement has been made to prolong the survival of SCLC patients. This study aimed to examine the mutation status of K-Ras (KRAS) and phosphatase and tensin homolog (PTEN) in SCLC patients in order to identify potential therapeutic targets for SCLC. Nineteen primary SCLC tumor specimens were enrolled in the study. Direct sequencing was perfonned to detect the mutations of KRAS exon 3 and PTEN exon 7 in the specimens. Kaplan- Meier and Cox regression analysis was performed to determine the overall survival (OS) of these SCLC patients. KRAS exon 3 mutation was found in 4 (21%) SCLC patients, and PTEN exon 7 mutation in only 1 (5%) SCLC patient. Kaplan Meier analysis showed that clinical stage and brain metastasis were significantly associated with OS (both P0.05). Cox proportional hazards regression model indicated that extensive stage of disease was the only independent negative prognostic factor for OS in SCLC patients. In conclusion, KRAS exon 3 and PTEN exon 7 mutations had no significant impact on OS of SCLC patients. Further study is still necessary to validate the molecular profiles of SCLC.

关键词： gene Ras small cell lung carcinoma prognosis

Expression of ΔDNMT3B Variants and Its Association with Estrogen/Progestogen Receptor Status in Breast Cancer

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Chinese Journal of Cancer Research 2009年第3期21卷 229-234页

作者： Ning-hong Liu lu yang Ming-lei Zhuo Jie Wang Shu-hang Wang Jun Zhao Hua Bai Key Laboratory of Carcinogenesis and Translational Research （Ministry of Education） Department of Thoracic Medical Oncology Peking University School of Oncology Beijing Cancer Hospital ＆ Institute Beijing 100142 China

Objective： Our previous study has showed that △DNMT3B is the predominant form of DNMT3B in non-small cell lung cancer （NSCLC）. In this study, we aimed to explore the expression patterns of the △DNMT3B variants in breast cancer and to identify whether the pattern was similar to that in NSCLC or not and its clinical significance. Methods： Expression of seven △DNMT3B variants in 59 breast cancer and the corresponding normal tissue was measured using RT-PCR. The correlations between the expressions of △DNMT3B variants and the clinical parameters including ER/PR status, clincopathologic feature and survivals were analyzed. Results： There were significant differences in the expression ratios of △DNMT3B1-7 variants between breast cancer tissues and normal tissues （P〈0.001）. The positive ratio of △DNMT3B1-7 variants were 66%, 71%, 17%, 51%, 76.2%, 50% and 61% in tumor tissue, respectively; while 16%, 8.4%, 3.38%, 3.38%, 11.8%, 13.5% and 5.08% in the corresponding normal tissue, which was different from the pattern of △DNMT3B1-7 expression in NSCLC （62%, 76%, 2.5%, 46%, 18%, 27% and 16% in tumor tissue, respectively; while 18%, 11%, 0%, 3.3%, 0%, 0% and 0% in normal lung tissue, respectively; P〈0.0001）. Expressions of △DNMT3B2, 3B4 and 3B7 were higher in the patients with negative estrogen receptor （ER） than those with positive estrogen receptor （P=0.035, P=0.0141 and P=0.0219, respectively）. △DNMT3B7 expression was higher for the patients with negative progestogen receptor （PR） compared to those with positive progestogen receptor （P=0.0379）. Expression ratio of △DNMT3B5 in stage Ⅲ tumors is lower than that in stage Ⅰ/Ⅱ ones （P= 0.041）. But we did not find any relation between the △DNMT3B variants and the patients＇ survival. Conclusion： The pattern of △DNMT3B variants in breast cancer is different from that in NSCLC. Expressions of △DNMT3B2, 3B4 and 3B7 are associated with estrogen receptors status. While △DNMT3B7 is associated with progestogen receptor. No relation

关键词： △DNMT3B variants RT-PCR Breast cancer Estrogen receptor Progestogen receptor

A two-stage parametric subspace model for efficient contrast-preserving decolorization

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Frontiers of Information Technology & Electronic Engineering 2017年第11期18卷 1874-1882页

作者： hong-yang lu Qie-gen LIU Yu-hao WANG Xiao-hua DENG Department of Electronic Information Engineering Nanchang University Nanchang 330031 China Institute of Space Science and Technology Nanchang University Nanchang 330031 China

The RGB2GRAY conversion model is the most popular and classical tool for image decolorization. A recent study showed that adapting the three weighting parameters in this first-order linear model with a discrete searching solver has a great potential in its c6nversion ability. In this paper, we present a two-step strategy to efficiently extend the parameter searching solver to a two-order multivariance polynomial model, as a sum of three subspaces. We show that the first subspace in the two-order model is the most important and the second one can be seen as a refinement. In the first stage of our model, the gradient correlation similarity （Gcs） measure is used on the first subspace to obtain an immediate grayed image. Then, Gcs is applied again to select the optimal result from the immettiate grayed image plus the second subspace-induced candidate images. Experimental results show the advantages of the proposed approach in terms of quantitative evaluation, qualitative evaluation, and algorithm complexity.

关键词： Color-to-gray conversion Subspace modeling Two-order polynomial model Gradient correlation similarity Discrete searching

Distribution and mapping of temperate savanna in the sandy lands of eastern China

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Science China Earth Sciences 2025年第1期68卷 128-141页

作者： Xiaoya LI Feng WANG Tao DUAN Kaijie yang Bin yang Chunmei WANG Xin TIAN Qi lu Institute of Ecological Conservation and Restoration Institute of Desertification Studies Chinese Academy of Forestry Institute of Desertification Institute of Desertification Studies Chinese Academy of Forestry Institute of Great Green Wall Satellite Application Centre for Ecology and Environment MEE Institute of Microelectronics Chinese Academy of Sciences Aerospace Information Research Institute Chinese Academy of Sciences Institute of Forest Resource Information Techniques Chinese Academy of Forestry

Savannas are distributed globally across various *** perform a significant socioeconomic role in regulating net primary productivity and the terrestrial carbon *** to the high spatial heterogeneity induced by the mixed distribution of trees and grasses,current global land-cover classification maps often classify savannas as forests or grasslands Temperate savannas in China cover a vast area,yet their spatial patterns and extent remain poorly *** study developed a novel remote sensing temperate savannas mapping method by integrating very high-resolution （VHR） unmanned aerial vehicle （UAV） and GF-6 satellite imagery based on the vegetation structure characteristics of temperate savannas using the FAO definition of *** and validation datasets were collected from VHR UAV imagery covering the main temperate savannah types with a ground area of 1818 *** fractional woody vegetation cover （FWVC） and fractional herbaceous vegetation cover （FHVC） in temperate savannas were estimated by integrating Classification and Regression Tree and Random Forest *** current distribution of temperate savannas in the Horqin and Otindag Sandy Lands of Eastern China was mapped to a unit area of 0.5 *** root-mean-square error （RMSE） values for the FWVC and FHVC models were 10.69% and 11.67%,and the R2values were 0.66 and 0.63,*** assessment indicated that the new temperate savanna map had high identification accuracy,with a Kappa coefficient of 0.70,overall accuracy of 0.88,and average accuracy of *** savannas in the sandy lands of eastern China were primarily distributed in the northern Horqin Sandy Land the central and eastern regions of the Otindag Sandy Land,and the transition zone from the southern slopes of the Greater Khingan Mountains to the plains,covering a total area of 3.27×10～6 *** main savannah types were elm,oak/maple,and shrub *** savannas were widely distributed in the Otindag Sandy

关键词： Temperate savanna UAV imagery GF-6 Fractional woody vegetation cover Fractional herbaceous vegetation cover Machine learning

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Electrocatalytic selective synthesis and tandem utilization of hydroxylamine

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Science China Chemistry 2025年

作者： Yuxuan lu Hengquan yang Engineering Research Center of Ministry of Education for Fine Chemicals School of Chemistry and Chemical Engineering Shanxi Key Laboratory of Green Catalytic Synthesis of Coal-based High Value Chemicals Shanxi University

The transformation of renewable nitrogen feedstock to nitrogenous chemicals plays a significant role in our modern society,considering the crucial impact of nitrogenous chemicals on life sciences,biomedicine,agriculture,and *** （NH2OH）,a typical nucleophile,has been widely served as the nitrogenous feedstock to produce nitrogenous *** NH2OH synthesis methods rely on harsh reaction conditions,leading to an unfavorable environmental *** contrast,nitric oxide electroreduction promises to produce NH2OH powered by renewable *** achievements have revealed the possibility of this process,but have also identified significant *** activity and selectivity are fundamentally limited by competing hydrogenation reactions,while the performance evaluation with high accuracy and reproducibility is ***,this review aims to provide insight into the mechanism of NH2OH electrosynthesis,especially regarding understanding reaction pathways and the design principle towards efficient ***,the performance evaluation parameters and standard measurement methods for NH2OH synthesis are summarized to screen efficient ***,the prospects and emerging opportunities to synthesize nitrogen-containing organic compounds by tandem reaction are *** review aims to provide a guide for evaluating and exploring efficient electrocatalytic systems for NH2OH production and utilization technology.

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冻融交替对土壤CO_2及N_2O释放效应的研究进展

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土壤 2010年第4期42卷 519-525页

作者：杨红露秦纪洪孙辉四川大学环境科学与工程系成都610065 成都大学城乡建设学院环境工程系成都610106

在秋冬交替和冬春交替时期高纬度地区和高海拔生态系统表层土壤常有冻融交替频繁发生。由于冻融交替作用通过改变土壤水热性质而对土壤物理、化学、生物学特性产生效应。冻结通常导致土壤团聚体破裂、微生物细胞及细根死亡,释放出活性... 详细信息

在秋冬交替和冬春交替时期高纬度地区和高海拔生态系统表层土壤常有冻融交替频繁发生。由于冻融交替作用通过改变土壤水热性质而对土壤物理、化学、生物学特性产生效应。冻结通常导致土壤团聚体破裂、微生物细胞及细根死亡,释放出活性较高的有机物,增强随后融解的土壤的反硝化和呼吸活性,从而影响土壤生物、生物化学过程以及生物地化循环。已有对苔原、泰加林等北极和亚北极生态系统的研究表明,土壤冻融交替次数、冻融极端温度、土壤水分、土壤团聚体结构变化等对CO2和N2O的释放通量影响较为显著,一般在冻融的最初几个循环温室气体排放会增加,随后会降至一个较为稳定的水平。目前,冻融循环变化背景下的温室气体排放研究主要是针对北方高纬度地区,而且对冻融交替影响土壤温室气体排放的机理研究也不够。我国面积广大的青藏高原高海拔地带在全球增温背景下,轻微增温会导致季节性冻土表层冻融交替次数增加,甚至冻土季节消失,加强全球增温背景下我国高山亚高山季节性冻土生态系统效应和过程研究,特别是土壤暖化导致的温室气体排放变化通量和变化机理的研究,对揭示全球变化的区域效应以及高海拔生态系统的管理都具有重要作用。

关键词：冻融循环温室气体土壤暖化亚高山土壤

Nanoparticles for the treatment of spinal cord injury

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Neural Regeneration Research 2025年第6期20卷 1665-1680页

作者： Qiwei yang Di lu Jiuping Wu Fuming Liang Huayi Wang Junjie yang Ganggang Zhang Chen Wang Yanlian yang Ling Zhu Xinzhi Sun Department of Orthopedic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina CAS Key Laboratory of Standardization and Measurement for Nanotechnology CAS Key Laboratory for Biomedical Effects of Nanomaterials and NanosafetyCAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyBeijingChina University of Chinese Academy of Sciences BeijingChina Department of Neurosurgery The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina

Spinal cord injuries lead to significant loss of motor, sensory, and autonomic functions, presenting major challenges in neural regeneration. Achieving effective therapeutic concentrations at injury sites has been a slow process, partly due to the difficulty of delivering drugs effectively. Nanoparticles, with their targeted delivery capabilities, biocompatibility, and enhanced bioavailability over conventional drugs, are garnering attention for spinal cord injury treatment. This review explores the current mechanisms and shortcomings of existing treatments, highlighting the benefits and progress of nanoparticle-based approaches. We detail nanoparticle delivery methods for spinal cord injury, including local and intravenous injections, oral delivery, and biomaterial-assisted implantation, alongside strategies such as drug loading and surface modification. The discussion extends to how nanoparticles aid in reducing oxidative stress, dampening inflammation, fostering neural regeneration, and promoting angiogenesis. We summarize the use of various types of nanoparticles for treating spinal cord injuries, including metallic, polymeric, protein-based, inorganic non-metallic, and lipid nanoparticles. We also discuss the challenges faced, such as biosafety, effectiveness in humans, precise dosage control, standardization of production and characterization, immune responses, and targeted delivery in vivo. Additionally, we explore future directions, such as improving biosafety, standardizing manufacturing and characterization processes, and advancing human trials. Nanoparticles have shown considerable progress in targeted delivery and enhancing treatment efficacy for spinal cord injuries, presenting significant potential for clinical use and drug development.

关键词： antioxidants axon regeneration biocompatible materials drug carriers nanoparticles nerve regeneration neuroinflammatory diseases neuroprotection spinal cord injury stem cells

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破碎岩体注浆加固后的力学特性研究

中国公路学报 2018年第10期31卷 284-291页

作者：刘人太郑卓李术才杨红鲁山东大学岩土与结构工程研究中心山东济南250061

基于理论分析建立破碎岩体注浆加固体力学模型,通过室内试验制作加固体试样并开展单轴抗压试验,分析破碎岩体含量、破碎程度、界面粗糙程度以及注浆材料黏结性能等因素对加固体强度的影响。试验表明:注浆加固体试样在单轴压缩破坏过程中... 详细信息

基于理论分析建立破碎岩体注浆加固体力学模型,通过室内试验制作加固体试样并开展单轴抗压试验,分析破碎岩体含量、破碎程度、界面粗糙程度以及注浆材料黏结性能等因素对加固体强度的影响。试验表明:注浆加固体试样在单轴压缩破坏过程中,其内部石块基本保持完整性,破坏主要产生在交界面以及注浆材料内部;当V_(BP)较大时,在碎石体的阻隔作用下,破裂面无法一次性产生,且多为剪切型破坏;当V_(BP)较小时,压缩破坏过程中直接形成了上下贯通的破裂面,且多为拉断型破坏;对于未加固的破碎岩体,其强度随V_(BP)增加而减小,且强度离散程度较大;加固后其强度随V_(BP)的增大而增大,整体强度大幅提高,同时呈现出明显的线性相关性;加固体强度随碎石数量的增加而减小,二者的线性相关性不显著;加固体强度随着注浆材料黏结强度的增加而增加,随交界面体密度的增加而减小;交界面体密度与黏结强度相互影响,总体强度是二者耦合作用的结果。基于加固体力学模型以及单轴抗压试验数据提出了加固体强度计算公式,该公式考虑了破碎岩体含量、破碎程度、界面粗糙程度以及注浆材料黏结性能等因素对加固体强度的影响。通过与实际试验数据比较,证明了该公式可以较好地对破碎岩体加固强度进行预测。

关键词：隧道工程注浆加固强度室内试验破碎岩体数据拟合