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Molecular mechanism of the inhibition effect of Celecoxib on corneal collagen degradation in three dimensions

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International Journal of Ophthalmology(English edition) 2012年第4期5卷 434-439页

作者： Wen-Song Zhang, hong-yan zhou Department of Ophthalmology the Second Hospital of Jilin University Changchun 130041 Jilin Province China Department of Ophthalmology China-Japan Union Hospital of Jilin University Changchun 130033 Jilin Province China

AIM: To clarify the molecular mechanism of Celecoxib on corneal collagen degradation and corneal ulcer. METHODS: Rabbit corneal fibroblasts were harvested and suspended in serum-free MEM. Type I collagen, DMEM, collagen reconstitution buffer and corneal fibroblast suspension were mixed on ice. The resultant mixture solidify in an incubator, after which test reagents and plasminogen was overlaid and the cultures were returned to the incubator. The supernatants from collagen gel incubations were collected and the amount of hydroxyproline in the hydrolysate was measured. Immunoblot analysis of MMP1, 3 and TIMP1, 2 was performed. MMP2, 9 was detected by the method of Gelatin zymography. Cytotoxicity Assay was measured. RESULTS: Celecoxib inhibited corneal collagen degradation in a dose and time manner; Celecoxib inhibited the IL-1 beta induced increases in proMMP1, 2, 3, 9 and active MMP1, 2, 3, 9 in a concentration-depended manner. Celecoxib can also inhibit the IL-1 beta induced increases in the TIMP1, 2. CONCLUSION: Celecoxib can inhibit corneal collagen degradation induced by IL-1 beta, this effect is the consequence of the reduction of MMP1, 2, 3, 9 and TIMP1, 2. The results of the present study provide new insight into Celecoxib in cornela ulcer treatment.

关键词： Celecoxib corneal ulcer IL-1β collagen degradation

Role of corneal collagen fibrils in corneal disorders and related pathological conditions

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 803-811页

作者： hong-yan zhou yan Cao Jie Wu Wen-Song Zhang Department of Ophthalmology China-Japan Union Hospital of Jilin University Department of Ophthalmology the Second Hospital of Jilin University

The cornea is a soft tissue located at the front of the eye with the principal function of transmitting and refracting light rays to precisely sense visual information. Corneal shape, refraction, and stromal stiffness are to a large part determined by corneal fibrils, the arrangements of which define the corneal cells and their functional behaviour. However, the modality and alignment of native corneal collagen lamellae are altered in various corneal pathological states such as infection, injury, keratoconus, corneal scar formation, and keratoprosthesis. Furthermore, corneal recuperation after corneal pathological change is dependent on the balance of corneal collagen degradation and contraction. A thorough understanding of the characteristics of corneal collagen is thus necessary to develop viable therapies using the outcome of strategies using engineered corneas. In this review, we discuss the composition and distribution of corneal collagens as well as their degradation and contraction, and address the current status of corneal tissue engineering and the progress of corneal cross-linking.

关键词： cornea collagen fibril collagen degradation collagen contraction tissue engineering

A Contact Energy Function Considering Residue Hydrophobic Environment and Its Application in Protein Fold Recognition

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Genomics, Proteomics & Bioinformatics 2005年第4期3卷 218-224页

作者： Mo-Jie Duan yan-hong zhou Hubei Bioinformatics and Molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan 430074 China.

The three-dimensional （3D） structure prediction of proteins ：is an important task in bioinformatics. Finding energy functions that can better represent residue-residue and residue-solvent interactions is a crucial way to improve the prediction accu- racy. The widely used contact energy functions mostly only consider the contact frequency between different types of residues; however, we find that the contact frequency also relates to the residue hydrophobic environment. Accordingly, we present an improved contact energy function to integrate the two factors, which can reflect the influence of hydrophobic interaction on the stabilization of protein 3D structure more effectively. Furthermore, a fold recognition （threading） approach based on this energy function is developed. The testing results obtained with 20 randomly selected proteins demonstrate that, compared with common contact energy functions, the proposed energy function can improve the accuracy of the fold template prediction from 20% to 50%, and can also improve the accuracy of the sequence-template alignment from 35% to 65%.

关键词： protein structure prediction fold recognition contact ,energy hydrophobic environment

Updating magnetic resonance imaging of small bowel:Imaging protocols and clinical indications

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World Journal of Gastroenterology 2008年第21期14卷 3403-3409页

作者： Jiong Zhu Jian-Rong Xu hong-Xia Gong yan zhou Department of Radiology Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127 China

High soft tissue contrast resolution,acquisition of multi-planar images and the possibility to obtain functional information make magnetic resonance an interesting imaging technique to evaluate the small bowel *** absence of ionizing radiation is an important feature of magnetic resonance imaging(MRI) examinations because inflammatory diseases such as Crohn's disease(CD) are studied most frequently,which are prevalent among children and young ***,using modern equipment and a rigorous technical approach,can offer detailed morphologic information and functional data on the small *** article discusses the MRI protocols for small bowel and the MR imaging findings of small bowel diseases,such as CD and small bowel neoplasms.

关键词： Magnetic resonance imaging Small bowel Crohn's Neoplasm

Discovery of Balmer broad absorption lines in the quasar LBQS 1206+1052

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Research in Astronomy and Astrophysics 2012年第4期12卷 369-382页

作者： Tuo Ji Ting-Gui Wang hong-yan zhou Hui-Yuan Wang Key Laboratory for Research in Galaxies and Cosmology University of Science and Technology of China （USTC） Hefei 230026 China Center for Astrophysics USTC Hefei 230026 China Polar Research Institute of China Shanghai 200136 China

We report the discovery of Balmer broad absorption lines （BALs） in the quasar LBQS 1206＋1052 and present a detailed analysis of the peculiar absorption line spectrum. Besides the Mg II λλ2796, 2803 doublet, BALs are also detected in the He I＊ multiplet at λλ2946, 3189, 3889 A arising from the metastable helium 2 3 S level, and in Hα and Hβ from the excited hydrogen H I＊ n = 2 level, which are rarely seen in quasar spectra. We identify two components in the BAL troughs of v ～ 2000 km s 1 width： One component shows an identical profile in H I＊, He I＊ and Mg II with its centroid blueshifted by v c ≈ 726 km s-1 . The other component is detected in He I＊ and Mg II with v c ≈ 1412 km s-1 . We estimate the column densities of H I＊, He I＊, and Mg II, and compare them with possible level population mechanisms. Our results favor the scenario that the Balmer BALs originate in a partially ionized region with a column density of N H ～ 10 21 10 22 cm-2 for an electron density of n e ～ 10 6 10 8 cm-3 via Lyα resonant scattering pumping. The harsh conditions needed may help to explain the rarity of Balmer absorption line systems in quasar spectra. With an i-band PSF magnitude of 16.50, LBQS 1206＋1052 is the brightest Balmer-BAL quasar ever reported. Its high brightness and unique spectral properties make LBQS 1206＋1052 a promising candidate for followup high-resolution spectroscopy, multi-band observations, and long-term monitoring.

关键词： galaxies active -- quasar absorption lines -- quasar individual (LBQS1206+1052)

Sn-MOFs/G composite materials for long cycling performance lithium ion batteries

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Rare Metals 2022年第5期41卷 1504-1511页

作者： Jian-Guo Zhao hong-yan zhou Zhuan Hu Ya-Wen Wu hong Jia Xian-Ming Liu School of Physics and Electronic Information Luoyang Normal UniversityLuoyang 471022China Henan Key Laboratory of Electromagnetic Transformation and Detection Luoyang Normal UniversityLuoyang 471022China School of Physics South China University of TechnologyGuangzhou 510640China School of Physics School of Physics and ElectronicsHunan UniversityChangsha 410082China School of Chemistry and Chemical Engineering Luoyang Normal UniversityLuoyang 471022China

Metal-organic frameworks(MOFs)are considered as one of the most important anode materials because of their controllable preparation,large specific surface area and controllable ***,low electronic conductivity causes u... 详细信息

关键词： lithium capacity cycling

Review of clinical and basic approaches of fungal keratitis

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International Journal of Ophthalmology(English edition) 2016年第11期9卷 1676-1683页

作者： Jie Wu Wen-Song Zhang Jing Zhao hong-yan zhou Department of Ophthalmology China-Japan Union Hospital of Jilin University Changchun 130033 Jilin Province China Department of Ophthalmology the Second Hospital of Jilin University Changchun 130000 Jilin Province China

Fungal keratitis（FK） is a serious disease which can cause blindness. This review has current information about the pathogenesis, limitations of traditional diagnosis and therapeutic strategies, immune recognition and the diagnosis and therapy of FK. The information of this summary was reviewed regularly and updated as what we need in the diagnosis and therapy of FK nowadays.

关键词： fungal keratitis pathogenesis diagnosis treatment

Effect of ligand troglitazone on peroxisome proliferator-activated receptor γ expression and cellular growth in human colon cancer cells

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World Journal of Gastroenterology 2006年第45期12卷 7263-7270页

作者： Mei Ming Jie-Ping Yu Xiang-Zhi Meng yan-hong zhou hong-Gang Yu He-Sheng kuo Department of Gastroenterology Renmin Hospital of Wuhan University Wuhan 430060 Hubei Province China Department of Pathophysiology and Pathology Medical College of Wuhan University Wuhan 430071 Hubei Province China

AIM： To investigate the effect of troglitazone on peroxisome proliferator-activated receptor γ （PPARγ） expression and cellular growth in human colon cancer HCT-116 and HCT-15 cells and to explore the related molecular mechanism.METHODS： Human colon cancer HCT-116 and HCT-15 cells cultured in vitro were treated with troglitazone. Reverse transcription-polymerase chain reaction （RT-PCR） and Western blot were employed to detect the effect of troglitazone on PPARy expression. The proliferative activity was determined by MTT assay, cell cycle and apoptosis were detected by flow cytometry. Apoptosisrelated genes, cell cycle regulatory genes and p53 were examined by RT-PCR and Western blot respectively. RESULTS： The expression of PPARy in colon cancer HCT-116 and HCT-15 cells was up-regulated by troglitazone. Troglitazone inhibited proliferation, induced apoptosis and cell cycle G1 arrest in colon cancer cells. Troglitazone induced p53 expression in HCT-116 cells, but not in HCT-15 cells. The down-regulation of survivin and bcl-2 was found in both cell lines and up-regulation of bax was found only in HCT-116 cells, being consistent with growth inhibition in HCT-116 cells but not in HCT-15 cells. Troglitazone increased expression of p21^WAF1/CIP1 （p21）, p27^KIP1 （p27） and reduced cyclin D1 in HCT-116 cells while only a minor decrease of cyclin D1 was found in HCT-15 cells. CONCLUSION： Troglitazone is an inductor of PPARγ in colon cancer cells and inhibits PPARγ-dependently proliferation, which may attribute to cell cycle G1 arrest and apoptosis in colon cancer cells. Troglitazone may induce p53-independent apoptosis and p53- dependent expression of p21 and p27. Depending on cell background, different activation pathways may exist in colon cancer cells.

关键词： Peroxisome proliferator-activated receptor γ Colon cancer Troglitazone Cellular growth Cell cycle Apoptosis

Prediction and Classification of Human G-protein Coupled Receptors Based on Support Vector Machines

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Genomics, Proteomics & Bioinformatics 2005年第4期3卷 242-246页

作者： Yun-Fei Wang Huan Chen yan-hong zhou Hubei Bioinformatics and Molecular Imaging Key Laboratory Huazhong University of Science and Technology Wuhan 430074 China.

A computational system for the prediction and classification of human G-protein coupled receptors （GPCRs） has been developed based on the support vector machine （SVM） method and protein sequence information. The feature vectors used to develop the SVM prediction models consist of statistically significant features selected from single amino acid, dipeptide, and tripeptide compositions of protein sequences. Furthermore, the length distribution difference between GPCRs and non-GPCRs has also been exploited to improve the prediction performance. The testing results with annotated human protein sequences demonstrate that this system can get good performance for both prediction and classification of human GPCRs.

关键词： GPCR prediction classification SVM