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Amniotic membrane transplantation for porous sphere orbital implant exposure

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第9期8卷 616-619页

作者： CHEN yan-hong cui hong-guang Department of Eye Center the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310015 China

Objective: This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods: A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 (5 males, 3 females; mean age 44.5 years). Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene (Medpor) orbital implants. The mean time from implantation to exposure was 1.1 months (range 0.8~2 months). All patients required surgical intervention. Results: The time of follow-up ranged from 3.0 to 28.0 months (mean 16.5 months). Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month (range 0.8~1.5 months). Conclusion: Exposed porous sphere implants were treated suc-cessfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.

关键词： Amniotic membrane transplantation Orbital implants Exposure

Novel organic dye sensitizers containing fluorenyl and biphenyl moieties for solar cells

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Chinese Chemical Letters 2017年第2期28卷 285-292页

作者： yan Xie Liang Han Cheng-Sheng Ge yan-hong cui Jian-Rong Gao College of Chemical Engineering Zhejiang University of Technology College of Chemistry and Materials Engineering Quzhou University

Three novel triarylamine dyes（AFL1-AFL3） containing fluorenyl and the biphenyl moieties have been designed and synthesized for application in dye-sensitized solar *** light-harvesting capabilities and photovoltaic performance of these dyes were investigated systematically through comparison of different π-*** dye with a furan linker exhibited a higher open-circuit voltage（VOC） and monochromatic incident photon-to-current conversion efficiency（IPCE） compared to thiophene and benzene ***,AFL3 containing a furan linker exhibited the maximum overall conversion efficiency of 5.81%（VOC = 760 mV,JSC = 11.36 mA cm^-2 and ff=0.68） under standard global AM 1.5 G solar condition.

关键词： Dye-sensitized solar cells Triarylamine Fluorenyl Biphenyl Furan bridge Photovoltaic performance

Identification of injury type using somatosensory and motor evoked potentials in a rat spinal cord injury model

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Neural Regeneration Research 2023年第2期18卷 422-427页

作者： Rong Li Han-Lei Li hong-yan cui Yong-Can Huang Yong Hu Department of Orthopedics and Traumatology The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong ProvinveChina Department of Neurosurgery Neuroscience CenterIntegrated Hospital of Traditional Chinese MedicineSouthern Medical UniversityGuangzhouGuangdong ProvinveChina Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinChina Shenzhen Engineering Laboratory of Orthopedic Regenerative Technologies Department of Spine SurgeryPeking University Shenzhen HospitalShenzhenGuangdong ProvinveChina Department of Orthopedics and Traumatology The University of Hong KongPokfulamHong Kong Special Administrative RegionChina

The spinal cord is at risk of injury during spinal *** intraoperative spinal co rd injury is identified early,irreve rsible impairment or loss of neurological function can be *** types of spinal cord injury result in damage to diffe rent spinal cord regions,which may cause diffe rent somatosensory and motor evoked potential signal res *** this study,we examined electrophysiological and histopathological changes between contusion,distra ction,and dislocation spinal cord injuries in a rat *** found that contusion led to the most severe dorsal white matter injury and caused considerable attenuation of both somatosensory and motor evoked *** resulted in loss of myelinated axons in the lateral region of the injured spinal cord along the rostrocaudal *** amplitude of attenuation in motor evoked potential responses caused by dislocation was greater than that caused by *** distraction injury,extracellular spaces were slightly but not significantly enlarged;somatosensory evoked potential res ponses slightly decreased and motor evoked potential responses were *** analysis showed that histological and electrophysiological findings we re significantly correlated and related to injury *** rative monitoring of both somatosensory and motor evoked potentials has the potential to identify iatrogenic spinal cord injury type during surgery.

关键词： contusion injury dislocation injury distraction injury electropnysiology heterogeneity histopathology injury mechanism motor evoked potential somatosensory evoked potential spinal cord injury

A translational study of somatosensory evoked potential time–frequency components in rats,goats,and humans

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Neural Regeneration Research 2021年第11期16卷 2269-2275页

作者： hong-yan cui Yi-Xin Wu Rong Li Guang-Sheng Li Yong Hu Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinChina Department of Orthopaedics and Traumatology The University of Hong KongHong Kong Special Administrative RegionChina Spinal Division Department of OrthopaedicsAffiliated Hospital of Guangdong Medical UniversityGuangdong ProvinceChina Department of Orthopaedics and Traumatology The University of Hong Kong-Shenzhen HospitalShenzhenGuangdong ProvinceChina

Somatosensory evoked potentials(SEPs)have been widely used to assess neurological function in clinical practice.A good understanding of the association between SEP signals and neurological function is helpful for precise diagnosis of impairment *** studies on SEPs have been reported in animal ***,few studies have reported the relationships between SEP waveforms in animals and those in *** this study,we collected normal SEP waveforms and decomposed them into specific time–frequency components(TFCs).Our results showed three stable TFC distribution regions in intact goats and rats and in *** we induced spinal cord injury in the animal models,a greater number of small TFC distribution regions were observed in the injured goat and rat groups than in the normal ***,there were significant correlations(Phong Kong/Hospital Authority hong Kong West Cluster(approval *** 05-312 T/975)on December 5,*** experiments were approved by the Committee on the Use of Live Animals in Teaching and Research of Li Ka Shing Faculty of Medicine of the University of hong Kong(approval *** 2912-12)on January 28,*** experiments were approved by the Animal Ethics Committee of Affiliated Hospital of Guangdong Medical University(approval ***2002132)on March 5,2018.

关键词： animal models chronic compression latency somatosensory evoked potentials spinal cord injury time-frequency analysis time-frequency components translational study

A virtual network mapping algorithm based on integer programming

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第12期14卷 899-908页

作者： Bo LU Jian-ya CHEN hong-yan cui Tao HUANG Yun-jie LIU Beijing Key Laboratory of Network System Architecture and Convergence Beijing University of Posts and Telecommunications

The virtual network(VN) embedding/mapping problem is recognized as an essential question of network virtualization. The VN embedding problem is a major challenge in this field. Its target is to efficiently map the virtual nodes and virtual links onto the substrate network resources. Previous research focused on designing heuristic-based algorithms or attempting two-stage solutions by solving node mapping in the first stage and link mapping in the second stage. In this study, we propose a new VN embedding algorithm based on integer programming. We build a model of an augmented substrate graph, and formulate the VN embedding problem as an integer program with an objective function and some constraints. A factor of topology-awareness is added to the objective function. The VN embedding problem is solved in one stage. Simulation results show that our algorithm greatly enhances the acceptance ratio, and increases the revenue/cost(R/C) ratio and the revenue while decreasing the cost of the VN embedding problem.

关键词： Virtual network embedding Integer programming Topology-awareness Network virtualization

Modeling deterministic echo state network with loop reservoir

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Journal of Zhejiang University-Science C(Computers and Electronics) 2012年第9期13卷 689-701页

作者： Xiao-chuan SUN hong-yan cui Ren-ping LIU Jian-ya CHEN Yun-jie LIU Beijing Key Laboratory of Network System Architecture and Convergence Beijing University of Posts and Telecommunications Beijing 100876China ICT Centre Commonwealth Scientific and lndustrial Research OrganizationSydney 1710Austraiia

Echo state network (ESN), which efficiently models nonlinear dynamic systems, has been proposed as a special form of recurrent neural network. However, most of the proposed ESNs consist of complex reservoir structures, leading to excessive computational cost. Recently, minimum complexity ESNs were proposed and proved to exhibit high performance and low computational cost. In this paper, we propose a simple deterministic ESN with a loop reservoir, i.e., an ESN with an adjacent-feedback loop reservoir. The novel reservoir is constructed by introducing regular adjacent feedback based on the simplest loop reservoir. Only a single free parameter is tuned, which considerably simplifies the ESN construction. The combination of a simplified reservoir and fewer free parameters provides superior prediction performance. In the benchmark datasets and real-world tasks, our scheme obtains higher prediction accuracy with relatively low complexity, compared to the classic ESN and the minimum complexity ESN. Furthermore, we prove that all the linear ESNs with the simplest loop reservoir possess the same memory capacity, arbitrarily converging to the optimal value.

关键词： Echo state networks Loop reservoir structure Memory capacity

Effects of different concentrations of nicotinamide on hematopoietic stem cells cultured in vitro

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World Journal of Stem Cells 2024年第2期16卷 163-175页

作者： yan Ren yan-Ni cui hong-Wei Wang The Second Clinical Medical College Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology The Second Hospital of Shanxi Medical UniversityTaiyuan 030001Shanxi ProvinceChina Department of Hematology Key Laboratory of Molecular Diagnosis and Treatment of Blood Diseases in Shanxi ProvinceTaiyuan 030001Shanxi ProvinceChina

BACKGROUND In vitro expansion to increase numbers of hematopoietic stem cells(HSCs)in cord blood could improve clinical efficacy of this vital ***(NAM)can promote HSC expansion ex vivo,but its effect on hematopoietic stem and progenitor cells(HSPCs,CD34^(+)CD38)and functional subtypes of HSCs-shortterm repopulating HSCs(ST-HSCs,CD34^(+)CD38CD45RACD49f^(+))and long-term repopulating HSCs(LT-HSCs,CD34^(+)CD38CD45RACD49f^(+)CD90^(+))is not yet *** a sirtuin 1(SIRT1)inhibitor,NAM participates in regulating cell adhesion,polarity,migration,proliferation,and ***,SIRT1 exhibits dual effects by promoting or inhibiting differentiation in different tissues or *** propose that the concentration of NAM may influence proliferation,differentiation,and SIRT1 signaling of *** To evaluate the effects and underlying mechanisms of action of different concentrations of NAM on HSC proliferation and *** CD34^(+)cells were purified from umbilical cord blood using MacsCD34 beads,and cultured for 10-12 d in a serum-free medium supplemented with cytokines,with different concentrations of NAM added according to experimental *** cytometry was used to detect phenotype,cell cycle distribution,and apoptosis of the cultured ***-time polymerase chain reaction was used to detect the transcription levels of target genes encoding stemness-related factors,che mokines,components of hypoxia pathways,and antioxidant ***-dihydro-fluorescein diacetate probes were used to evaluate intracellular production of reactive oxygen species(ROS).Determination of the effect of different culture conditions on the balance of cytokine by cytometric bead *** Compared with the control group,the proportion and expansion folds of HSPCs(CD34^(+)CD38)incubated with 5 mmol/L or 10 mmol/L NAM were significantly increased(all P<0.05).The ST-HSCs ratio and fold expansion of the 5 mmol/L NAM group were significantly

关键词： Hematopoietic stem cells Nicotinamide Concentration Proliferation Differentiation Sirtuin 1

Utility of somatosensory and motor-evoked potentials in reflecting gross and fine motor functions after unilateral cervical spinal cord contusion injury

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Neural Regeneration Research 2021年第7期16卷 1323-1330页

作者： Rong Li Zu-Cheng Huang hong-yan cui Zhi-Ping Huang Jun-Hao Liu Qing-An Zhu Yong Hu Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical CollegeTianjinChina Department of Spine Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Orthopedics and Traumatology The University of Hong KongHong Kong Special Administrative RegionChina Department of Orthopedics and Traumatology The Hong Kong University Shenzhen HospitalShenzhenGuangdong ProvinceChina

Fine motor skills are thought to rely on the integrity of ascending sensory pathways in the spinal dorsal column as well as descending motor pathways that have a neocortical ***, the neurophysiological processes underlying communication between the somatosensory and motor pathways that regulate fine motor skills during spontaneous recovery after spinal cord contusion injury remain ***, we established a rat model of cervical hemicontusive injury using C5 laminectomy followed by contusional displacement of 1.2 mm(mild injury) or 2.0 mm(severe injury) to the C5 spinal *** recordings were performed on the brachial muscles up to 12 weeks after injury to investigate the mechanisms by which spinal cord pathways participate in motor *** spinal cord contusion injury, the amplitudes of somatosensory and motor-evoked potentials were reduced, and the latencies were *** forelimb open field locomotion test, grooming test, rearing test and Montoya staircase test revealed improvement in *** increasing time after injury, the amplitudes of somatosensory and motor-evoked potentials in rats with mild spinal cord injury increased gradually, and the latencies gradually *** comparison, the recovery times of somatosensory and motor-evoked potential amplitudes and latencies were longer, and the recovery of motor function was delayed in rats with severe spinal cord *** analysis revealed that somatosensoryevoked potential and motor-evoked potential parameters were correlated with gross and fine motor function in rats with mild spinal cord contusion *** contrast, only somatosensory-evoked potential amplitude was correlated with fine motor skills in rats with severe spinal cord *** results show that changes in both somatosensory and motor-evoked potentials can reflect the changes in gross and fine motor functions after mild spinal cord contusion injury, and that the change in somatosen

关键词： central nervous system motor-evoked potential motor function regeneration repair somatosensory-evoked potential spinal cord spinal cord injury

The Messenger RNA and Long Non-coding RNA Expression Profiles in Ectopic and Eutopic Endometrium Provide Novel Insights into Endometriosis

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Reproductive and Developmental Medicine 2019年第1期3卷 11-17页

作者： Song-Ping Liu Xin Tian hong-yan cui Qiong Zhang Ke-Qin Hua Department of Gynecology Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 200090China Department of Obstetrics and Gynecology Zhenjiang Maternal and Child Health HospitalZhenjiang 212001China

Objective:To establish the messenger RNA(mRNA)and long non-coding RNA(lncRNA)expression profiles in ectopic and eutopic endometrium and provide novel insights into ***:The mRNA and lncRNA expression profiles were tested using high-throughput sequencing technology in ectopic and eutopic endometrium with endometriosis and normal endometrium without *** potential targeted lncRNAs were annotated by analyzing the correlation between lncRNA and mRNA expression to better understand the pathogenesis of ***:In ectopic compared with normal endometrium,a total of 2,188 mRNAs and 1,200 lncRNAs were differentially expressed with a fold-change(FC)≥*** eutopic compared with normal endometrium,a total of 2,324 mRNAs and 695 lncRNAs were differentially expressed with an FC≥*** ectopic compared with eutopic endometrium,a total of 2,223 mRNAs and 511 lncRNAs were differentially expressed with an FC≥*** analysis indicated that the differentially expressed mRNAs were enriched in the biological processes and signaling pathways involved in *** addition,we constructed a gene coexpression network based on the dysregulated lncRNAs in both ectopic endometrium and eutopic endometrium,combined with their coexpressed mRNAs to simulate the complex ***:This study describes the first-to-integrate analysis of the differential expression profiles of mRNAs and lncRNAs,including analyses between ectopic and normal endometrium,eutopic and normal endometrium,and ectopic and eutopic endometrium,which provides new insights to investigate the pathogenesis of endometriosis and explore novel diagnostic biomarkers and therapeutic targets.

关键词： Endometriosis Gene Profile Long Non-coding RNA Messenger RNA RNA Sequencing