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共载吲哚菁绿和Nrf2-siRNA的多功能纳米粒子对抗口腔鳞状细胞癌的体外作用研究

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口腔医学研究 2024年第5期40卷 422-428页

作者：付凯钰常云汉史恩宇史澍睿天津市口腔医院儿童口腔科、南开大学医学院天津300041 天津市口腔功能重建重点实验室天津300041 天津医科大学口腔医院综合科天津300070 天津市口腔软硬组织修复再生重点实验室天津300070

目的:构建共载吲哚菁绿(indo cyanine green,ICG)和核因子E2相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)-短干扰RNA(small interfering RNA,siRNA)的多功能纳米载药粒子,并探究其联合光热疗法与抗氧化抑制作用增效的光动力疗法协同对抗口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)的体外作用与机制。方法:利用超声乳化法和静电力吸附作用制备基于聚氨基酯/聚乳酸-羟基乙酸共聚物且共载ICG和Nrf2-siRNA的纳米粒子PPI-siRNA。表征PPI-siRNA纳米粒子的形貌、粒径大小和分布、表面电位及其载药情况;评价PPI-siRNA纳米粒子对ICG和Nrf2-siRNA的胞内递送以及Nrf2-siRNA逃逸溶酶体吞噬的性能;通过检测舌鳞状细胞癌细胞SCC-25随激光照射的升温效应、热休克蛋白60表达和活性氧生成水平考察PPI-siRNA纳米粒子的光热和光动力性能;考察细胞内Nrf2及其调控的下游基因谷氨酸-半胱氨酸连接酶修饰亚基和谷氨酸-半胱氨酸连接酶的表达水平,从而探究Nrf2-siRNA助力光动力效应的作用机制;运用噻唑蓝比色法考察PPI-siRNA联合激光照射对SCC-25的细胞毒性作用。结果:PPI-siRNA纳米粒子呈形貌规则的球状结构,粒径约为180 nm,并成功共载ICG与Nrf2-siRNA;PPI-siRNA纳米粒子可以高效递送ICG和Nrf2-siRNA入胞,且可以确保Nrf2-siRNA逃逸溶酶体吞噬,从而发挥基因沉默作用;PPI-siRNA纳米粒子具备良好的光热和光动力性能,Nrf2-siRNA可以通过下调抗氧化蛋白和基因的表达显著提升光学疗法的抗肿瘤效率。结论:PPI-siRNA纳米粒子可以联合光热疗法与基因沉默作用增效的光动力疗法并有效抑制SCC-25的体外生长,在OSCC的临床治疗方面具有广阔的应用前景。

关键词：口腔鳞状细胞癌纳米粒子光热/光动力疗法基因沉默抗氧化抑制效应

肺部感染致脓毒症患者脑功能障碍的危险因素分析

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医药论坛杂志 2024年第13期45卷 1345-1348页

作者：王泽帅赵世龙刘秋红郑州大学第一附属医院呼吸与危重症医学科郑州450052

目的探讨肺部感染致脓毒症患者脑功能障碍的危险因素。方法选取2020年3月—2022年9月郑州大学第一附属医院收治的肺部感染致脓毒症患者142例,根据是否发生脓毒症所致脑功能障碍(sepsis induced brain dysfunction,SIBD)分为SIBD组77例与... 详细信息

目的探讨肺部感染致脓毒症患者脑功能障碍的危险因素。方法选取2020年3月—2022年9月郑州大学第一附属医院收治的肺部感染致脓毒症患者142例,根据是否发生脓毒症所致脑功能障碍(sepsis induced brain dysfunction,SIBD)分为SIBD组77例与非SIBD组65例,比较两组一般资料与实验室指标,采用logsitic回归分析SIBD的独立危险因素。结果SIBD组脓毒症休克、机械通气、急性呼吸窘迫综合征患者比例以及ICU住院时间、SOFA评分、APACHEII评分高于非SIBD组(P<0.05);SIBD组血红蛋白低于非SIBD组,尿素氮、血肌酐、凝血酶原国际比值、降钙素原、S100β蛋白、神经元特异性烯醇化酶高于非SIBD组(P<0.05);SOFA评分(OR=1.583,95%CI:1.234~5.281)、GCS评分(OR=2.181,95%CI:2.358~9.731)、S100β蛋白(OR=1.324,95%CI:1.253~4.732)水平是肺部感染致脓毒症患者发生SIBD的独立危险因素(P<0.05)。结论SOFA评分、GCS评分、S100β蛋白水平是预测SIBD的主要危险因素,加强上述指标的动态监测,可能是预防SIBD发生、发展的有效措施。

关键词：肺部感染脓毒症脑功能障碍临床特征危险因素

国产脊髓电刺激系统在小尾寒羊植入的长期安全性和组织相容性研究

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中国疼痛医学杂志 2024年第8期30卷 568-577页

作者：章沿锋蔡世宏柳成江李云泽彭志友冯智英浙江大学医学院附属第一医院疼痛科杭州310003 台州市中心医院(台州学院附属医院)疼痛科台州318000

目的:验证国产脊髓电刺激(spinal cord stimulation,SCS)系统的可操作性、长期安全性和组织相容性。方法:成年雄性小尾寒羊9只,其中3只为短期植入组(S组)即SCS电极植入2周;另6只为长期植入组(L组)即SCS电极和脉冲发生器植入长达26周。... 详细信息

目的:验证国产脊髓电刺激(spinal cord stimulation,SCS)系统的可操作性、长期安全性和组织相容性。方法:成年雄性小尾寒羊9只,其中3只为短期植入组(S组)即SCS电极植入2周;另6只为长期植入组(L组)即SCS电极和脉冲发生器植入长达26周。观察术前(T_(0))、术后1周(T_(1))、2周(T_(2))、4周(T_(3))、8周(T_(4))、12周(T_(5))、20周(T_(6))、26周(T_(7))实验羊的行为学改变、实验室检查、设备电阻抗值、高频低频刺激情况、电极移位等,S组和L组分别于术后2周和26周取SCS周围组织行苏木精-伊红染色行病理性检查。结果:S组和L组植入SCS后运动正常。与术前相比,S组与L组的血常规、肝肾功能和凝血功能无明显变化(P>0.05)。但与术前相比,L组实验羊血糖水平在术后2、4、8、12周均明显降低(P<0.05)。术中及术后阻抗值正常,电极未见移位。刺激节段脊髓形态结构正常,未见明显坏死、水肿、缺血、炎症等病理改变。结论:国产植入式SCS系统可操作性良好,长期植入后具有良好的生物相容性和安全性,符合临床应用需求。

关键词：脊髓电刺激生物安全性组织相容性小尾寒羊

The relationship between physical activity in early pregnancy and hypertensive disorders of pregnancy: a cohort study in Chinese women

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World Journal of Emergency Medicine 2023年第3期14卷 204-208页

作者： Qian Lu shi-jiao Yan Huan-jun Chen Xiong-fei Pan Yi-xiang Ye xing-yue Song Ri-xing Wang Chuan-zhu Lyu International School of Public Health and One Health Hainan Medical UniversityHaikou 570100China Research Unit of Island Emergency Medicine Chinese Academy of Medical SciencesHainan Medical UniversityHaikou 570100China Section of Epidemiology and Population Health Ministry of Education Key Laboratory of Birth Defects and Related Diseases of Women and ChildrenWest China Second University Hospital&West China Biomedical Big Data CenterWest China HospitalSichuan UniversityShuangliu Institute of Women's and Children's HealthShuangliu Maternal and Child Health HospitalChengdu 610041China Department of Epidemiology and Biostatistics School of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430000China Department of Emergency Hainan Clinical Research Center for Acute and Critical Diseasesthe Second Affi liated Hospital of Hainan Medical UniversityHaikou 570100China Emergency Medicine Center Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Ministry of Education Key Laboratory of Emergency and Trauma Hainan Medical UniversityHaikou 570100China

BACKGROUND:We aimed to examine prospective associations between diff erent intensities and diff erent types of physical activity(PA)in early pregnancy and hypertensive disorders of pregnancy(HDP)among Chinese ***:A total of 6,820 pregnant women from the Tongji-Shuangliu Birth Cohort were included in this *** pregnancy physical activity questionnaire(PPAQ)was used to assess PA,including household/caregiving,occupational,sports/exercise,and transportation activities in the first trimester of *** diagnosis of HDP was collected,including gestational hypertension(GH)and preeclampsia(PE).Data were analyzed by unconditional multivariate logistic regression,and the odds ratio(OR)and 95%confi dence interval(CI)were ***:A total of 178(2.6%)of the 6,820 women were diagnosed with HDP,of which 126(1.8%)were GH and 52(0.8%)were ***,we found no association between PA in early pregnancy and PE.A trend toward lower risk was found only among women with GH and among those with higher levels of moderate-to-vigorous intensity physical activity(MVPA)(adjusted OR 0.54,95%CI 0.31–0.96).No association was observed between PA and HDP in early pregnancy,regardless of diff erent intensities or types of ***:MVPA in the first trimester is an influencing factor of *** pregnant women to engage in MVPA in the fi rst trimester may help to prevent GH.

关键词： Physical activity Gestational hypertension Preeclampsia Prospective cohort study

Accurate Registration of Remote Sensing Images Based on Local Optimal Transformation

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Journal of Beijing Institute of Technology 2019年第2期28卷 371-382页

作者： Bo Wang Changqing Li shi Tang Zhiqiang Zhou hong Zhao School of Automation Beijing Institute of TechnologyBeijing 100081China Beijing Urban Construction Design&Development Group Co. Ltd.Beijing 100071China Beijing Navigation Control Technology Co. Ltd.Beijing 102200China

As the basic work of image stitching and object recognition,image registration played an important part in the image processing *** previous work in registration accuracy and realtime performance progressed very slowly,especially in registrating images with line *** innovative method for image registration based on lines is proposed,it can effectively improve the accuracy and real-time performance of image *** line feature can deal with some registration problems where point feature does not *** registration process is divided into two *** first part determines the rough registration transformation relation between reference image and test *** the similarity degree among different transformation and modified nonmaximum suppression(MNMS)algorithms are obtained,which produce local optimal solution to optimize the rough registration *** final optimal registration relation can be obtained from two registration parts according to the match *** experimental results show that the proposed method makes a more accurate registration relation and performs better in real-time situation.

关键词： initial registration relationship accurate registration relationship similarity degree local optimal transformation modified non-maximum suppression(MNMS)algorithm

Nonlinear optical properties of CsPbCl_(x)Br_(3-x)nanocrystals embedded glass

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Photonics Research 2021年第9期9卷 1767-1774页

作者： CHENJING QUAN XIAO xing SIHAO HUANG MENGFEIFEI JIN TONGCHAO shi ZEYU ZHANG WEIDONG XIANG ZHANSHAN WANG YUXIN LENG School of Physics Science and Engineering Tongji UniversityShanghai 200092China State Key Laboratory of High Field Laser Physics and CAS Center for Excellence in Ultra-intense Laser Science Shanghai Institute of Optics and Fine Mechanics(SIOM)Chinese Academy of Sciences(CAS)Shanghai 201800China College of Chemistry and Materials Engineering Wenzhou UniversityWenzhou 325035China Hangzhou Institute for Advanced Study University of Chinese Academy of SciencesHangzhou 310024China School of Physics and Electronics Shandong Normal UniversityJinan 250014China

All-inorganic perovskite has attracted significant attention due to its excellent nonlinear optical *** and low-toxic perovskite materials have great application prospects in optoelectronic ***,we study the nonlinear optical properties of CsPbCl_(x)Br_(3-x)(x=1,1.5,2)nanocrystals(NCs)glass by open-aperture *** is found that the two-(2 PA)and three-photon absorption(3 PA)intensity can be adjusted by the treatment temperature and the ratio of halide *** perovskite NCs glass treated at a high temperature has better crystallinity,resulting in stronger nonlinear absorption *** addition,the value of the 2 PA parameter of CsPbCl_(1.5)Br_(1.5)NCs glasses decreases when the incident pump intensity increases,which is ascribed to the saturation of 2 PA and population ***,the research results show that the 2 PA coefficient(0.127 cm GW-1)and 3 PA coefficient(1.21×10^(-5)cm^(3)GW-2)of CsPbCl_(1)Br_(2)NCs glass with high Br anion content are larger than those of CsPbCl_(2)Br_(1)and CsPbCl_(1.5)Br_(1.5)NCs *** is mainly due to the greater influence of Br anions on the symmetry of the perovskite structure,which leads to the redistribution of delocalized *** revealed adjustable nonlinear optical properties of perovskite NCs glass are essential for developing stable and high-performance nonlinear optical devices.

关键词： glass perovskite glasses

ROLE OF CASPASE-3 IN ACUTE LIGHT DAMAGE TO RETINA OF RATS

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Chinese Medical Sciences Journal 2007年第1期22卷 44-48页

作者： Xiao Wang shi-xing Hu Wei Li Shao-chun Lin Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 Laboratory Animal Center Guangzhou University of Traditional Medicine Guangzhou 510405

Objective To investigate the role of Caspase-3 in retinal damage caused by hght exposure in rats. Methods Light injury to retina was induced by persistent exposure to illumination （intensity ： 30 000 ± 50 lux） of operating microscope for 30 minutes in the right eyes of Sprague-Dawley rats. The pathological changes of retina were observed under optical and electron microscopies at different time points, which were 6 hours, 1,3,7, and 15 days after the hght exposure. Apoptosis of retinal cells was analyzed by flow cytometry. The activity of Caspase-3 was evaluated by using the Caspase-3 assay kit. At the same time, the expression of Caspase-3 protease was determined with Western blot analysis. Results The examination results of optical and transmission electron microscopes showed that edema of inner and outer segments of the retina, especially the chondriosome inside the inner segment, became obvious 6 hours after the light exposure. The change was deteriorated along with the increasing time. The structures of the discoidal valve dissociated in the outer segment simultaneously. Disorderly arranged nuclei, karyopycnosis, and thinning in the outer nuclear layer were observed. The retinal pigment epithelium almost disappeared during the later stage. The staining results of Annexin-V combined with PI demonstrated that the proportion of apoptotic cells increased with time. The proportion between 7th day （82.7%） and 15th day （80.4%）, however, showed no significant difference. Caspase-3 became remarkably active with the lapse of time, which increased from 0.02 at 6th hour to the peak of 9. 8 at 7th day before it started to descend. The Western blot detected a expression of the active form of Caspase-3 at 7th day and 15th day. Conclusion Apoptosis of photoreceptor cells is markedly involved in the light damage and Caspase-3 protease may play an important role in the apoptotic process of the retina after light exposure in rats.

关键词： retina light damage Caspase-3 apoptosis rat

面向碳中和目标的中国工业部门减排路径与战略选择

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管理科学学报 2024年第4期27卷 1-20页

作者：陈诗一王畅郭越复旦大学经济学院上海200433 复旦大学国际金融学院上海200433 中国科学院地理科学与资源研究所中国科学院区域可持续发展分析与模拟重点实验室北京100101 中国科学院大学资源与环境学院北京100049

工业部门(包含电力工业)是中国碳排放量最多的国民经济部门.探寻工业部门合理有效的碳减排路径是中国碳达峰、碳中和目标顺利实现的关键.本文分解了中国2000年—2019年工业碳排放历史演变路径的驱动因素,预测了三种情景下2019年—2060... 详细信息

工业部门(包含电力工业)是中国碳排放量最多的国民经济部门.探寻工业部门合理有效的碳减排路径是中国碳达峰、碳中和目标顺利实现的关键.本文分解了中国2000年—2019年工业碳排放历史演变路径的驱动因素,预测了三种情景下2019年—2060年工业碳排放的潜在演变路径,估计了工业部门碳中和进程中所需的投资额,讨论了不同减排时点选择下投资需求的异质性.研究发现:1)规模效应和效率效应是历史演变路径中最关键的驱动因素,而化石能源清洁转化效应、化石能源内部结构效应和清洁能源替代效应的影响较小;2)非电力工业未来核心的碳减排技术路径为能源效率提升和清洁能源消费替代,电力工业未来核心的碳减排技术路径则为清洁能源发电替代;3)在当前的技术水平下,工业部门碳中和的进程中需要超过300万亿元的巨额投资,所需投资主要集中于高排放的非电力工业.本文最后提出了“以技术为核心,以市场为机制,以政策为引导”的中国工业部门实现碳中和的战略选择.

关键词：工业碳排放碳中和路径指数分解情景分析边际减排成本

A typical Riedel Shear Pattern of active faults in the Laolongwan Basin， Northeastern Tibetan Plateau

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Journal of Earth Science 2024年

作者： Kai-Xun WANG Xiao-Gang LI Wei shi Xiang-You FENG Qian YANG Jie LI Zheng-Jian XU Zhou-Jun PENG Shao-Hua XU Yi-Xue XIONG Mao LIN School of Petroleum Engineering， Chongqing University of Science and Technology Chongqing Key Laboratory of Complex Oil and Gas Field Exploration and Development， Chongqing University of Science and Technology Geophysics Research Institute of Jiangsu Oilfield Company，SINOPEC SinoProbe Laboratory， Chinese Academy of Geological Sciences Academy of Dazu Rock Carvings

Riedel shear system， consists of some different oriented faults and derivative structures， is an important pattern of tectonic activity and stress regulation， which has been widely applied to the interpretation of intracontinental deformation. The Laolongwan Basin， located in the western Haiyuan fault zone at the northeastern Tibetan Plateau， is a key area to study the Cenozoic intracontinental deformation in the northeastern plateau， which formed a complex active fault system during the Cenozoic. However， the activity of these faults and their kinematic mechanism remain unclear. In this contribution， based on detailed structural interpretation of remote sensing image， field observations and OSL dating analysis， we propose a Riedel Shear model of active fault system in the Laolongwan basin. Our observations show that this active fault system is consist of four major faults， including the left strike-slip Hasi Shan Fault and Zihong Shan Fault with thrusting characteristics， the Southern Zihong Shan thrust fault and the Mijia Shan normal fault. The fault offset and OSL dating analyses suggest that the left-lateral slip rate of the Hasi Shan Fault is ～ 2.60-3.01 mm/a since ca. 15 ka， whereas the Zihong Shan Fault is ～ 1.10-1.13 mm/a since ca. 14 ka. Fault-slip vectors analyses indicate that the active fault system related to the Riedel Shear in the Laolongwan Basin was controlled by the regional ENE-WSW compressive stress. This compression also caused the significant left-lateral strike-slip movement along the Haiyuan Fault zone at the same time， which might result from the northeastward continuous expanding of the Tibetan Plateau during the late Cenozoic.

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