检索结果-南通市图书馆

Transcriptome analysis reveals tumor antigen and immune subtypes of melanoma

在线全文

学校读者我要写书评

暂无评论

Oncology Research 2023年第3期31卷 389-403页

作者： JIAHENG XIE MENGMENG ou PAN YU DAN WU QIKAI TANG YUAN CAO JING HANG LU YIN TINGhong xiang MING WANG JINGPING SHI Department of Burn and Plastic Surgery The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China Department of Rheumatology and Immunology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008China Department of Neurosurgery The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China Fourth School of Clinical Medicine Nanjing Medical UniversityNanjing210029China Department of Ultrasound in Medicine The First Affiliated Hospital of Nanjing Medical UniversityJiangsu Province HospitalNanjing210029China

Purpose:To screen potential tumor antigens for melanoma vaccine development and identify different immune subtypes.Methods:Transcriptional data(HTSEQ-FPKM)and clinical information of a 472 Melanoma cohort GDC TCGA Melanoma(SKCM)were downloaded from the UCSC XENA website(http://xena.***.edu/).Subsequently,transcriptome data and clinical information of 210 melanoma cohort GSE65904 were downloaded from Gene Expression Omnibus(GEO),a large global public database.All the transcriptome expression data matrices were log2 transformed for subsequent analysis.GEPIA,TIMER,and IMMPORT databases are also used for analysis.Cell function experiments were performed to validate the role of the IDO1 gene in melanoma cell line A375.Results:our study provides potential tumor antigens for vaccine development in melanoma patients:GZMB,GBP4,CD79A,APOBEC3F,IDO1,JCHAIN,LAG3,PLA2G2D,XCL2.In addition,we divide melanoma patients into two immune subtypes that have significant differences in tumor immunity and may have different responses to vaccination.In view of the unclear role of IDO1 in melanoma,we selected IDO1 for cell assay validation.Cell function assay showed that IDO1 was significantly overexpressed in the melanoma A375 cell line.After IDO1 knockdown,the activity,invasion,migration and healing ability of A375 cell lines were significantly decreased.Conclusion:our study could provide a reference for the development of vaccines for melanoma patients.

关键词： Melanoma mRNA vaccine Immune microenvironment Immunotherapy Bioinformatics analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

进口再生橡胶的固体废物属性鉴别方法探讨

中国口岸科学技术 2024年第4期6卷 34-40页

作者：蒋一昕袁敏朱海欧吴璟张彰崔祥慧张桂珍南京海关工业产品检测中心南京210019 南京金检检验有限公司南京210019

为保证进口再生橡胶原料质量,防止硫化橡胶废碎料等属于我国禁止进口的固体废物以再生橡胶原料名义进口,因此需对进口再生橡胶原料实施固体废物属性鉴别。目前针对进口再生橡胶的固体废物属性鉴别尚无统一的标准方法。本文通过外观检查... 详细信息

为保证进口再生橡胶原料质量,防止硫化橡胶废碎料等属于我国禁止进口的固体废物以再生橡胶原料名义进口,因此需对进口再生橡胶原料实施固体废物属性鉴别。目前针对进口再生橡胶的固体废物属性鉴别尚无统一的标准方法。本文通过外观检查、红外光谱分析、裂解气相色谱-质谱和再生橡胶的性能指标测试等手段,考察进口再生橡胶原料的均匀程度,确定回收橡胶的种类,测试其性能参数,结合生产工艺和原料来源等因素进行综合判断,为准确鉴别再生橡胶固体废物属性提供了参考依据。

关键词：再生橡胶固体废物属性鉴别

Instant Edit Propagation on Images Based on Bilateral Grid

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2019年第8期61卷 643-656页

作者： Feng Li Chaofeng ou Yan Gui Lingyun xiang Hunan Provincial Key Laboratory of Intelligent Processing of Big Data on Transportation Changsha University of Science and TechnologyChangsha410114China School of Computer and Communication Engineering Changsha University of Science and TechnologyChangsha410114China School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore639798Singapore

The ability to quickly and intuitively edit digital content has become increasingly important in our everyday life.However,existing edit propagation methods for editing digital images are typically based on optimization with high computational cost for large inputs.Moreover,existing edit propagation methods are generally inefficient and highly time-consuming.Accordingly,to improve edit efficiency,this paper proposes a novel edit propagation method using a bilateral grid,which can achieve instant propagation of sparse image edits.Firstly,given an input image with user interactions,we resample each of its pixels into a regularly sampled bilateral grid,which facilitates efficient mapping from an image to the bilateral space.As a result,all pixels with the same feature information(color,coordinates)are clustered to the same grid,which can achieve the goal of reducing both the amount of image data processing and the cost of calculation.We then reformulate the propagation as a function of the interpolation problem in bilateral space,which is solved very efficiently using radial basis functions.Experimental results show that our method improves the efficiency of color editing,making it faster than existing edit approaches,and results in excellent edited images with high quality.

关键词： Instant edit propagation bilateral grid radial basis function image editing

Population attributable risks of cigarette smoking for deaths of all causes, all cancers and other chronic diseases among adults aged 40-74 years in urban Shanghai, China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Cancer Research 2015年第1期27卷 59-65页

作者： Ying-Ying Wang Wei Zhang hong-Lan Li Jing Gao Yu-Ting Tan Yu-Tang Gao Xiao-ou Shu Wei Zheng Yong-Bing xiang School of Public Health Fudan University Shanghai Cancer Institute Renji Hospital Shanghai Jiaotong University School of Medicine Division of Epidemiology Department of Medicine Vanderbilt Epidemiology Center Vanderbilt-Ingram Cancer Center Vanderbilt University School of Medicine

Objective： To evaluate the population attributable risks （PARs） between cigarette smoking and deaths of all causes, all cancers, lung cancer and other chronic diseases in urban Shanghai. Methods： In total, 61,480 men aged 40-74 years from 2002 to 2006 and 74,941 women aged 40-70 years from 1997 to 2000 were recruited to undergo baseline surveys in urban Shanghai, with response rates of 74.0% and 92.3%, respectively. A Cox proportional hazards regression model was used to estimate relative risks （RRs） and 95% confidence intervals （95% CIs） of deaths associated with cigarette smoking. PARs and 95 % CIs for deaths were estimated from smoking exposure rates and the estimated RRs. Results： Cigarette smoking was responsible for 23.9% （95% CI： 19.4-28.3%） and 2.4% （95% Ch 1.6- 3.2%） of all deaths in men and women, respectively, in our study population. Respiratory disease had the highest PAR in men [37.5% （95% CI： 21.5-51.6%）], followed by cancer [31.3% （95% Ch 24.6-37.7%）] and cardiovascular disease （CVD） [24.1% （95% CI： 16.7-31.2%）]. While the top three PARs were 12.7% （95% CI： 6.1-19.3%）, 4.0% （95% CI： 2.4-5.6%）, and 1.1% （95% CI： 0.0-2.3%）, for respiratory disease, CVD, and cancer, respectively in women. For deaths of lung cancer, the PAR of smoking was 68.4% （95% CI： 58.2- 76.5%） in men. Conclusions： In urban Shanghai, 23.9% and 2.4% of all deaths in men and women could have been prevented if no people had smoked in the area. Effective control programs against cigarette smoking should be strongly advocated to reduce the increasing smoking-related death burden.

关键词： Population attributable risk （PAR） smoking mortality cohort study all causes death cancer death lung cancer

不同低温时间无水保活对中国圆田螺存活、营养组成、消化酶活性及抗氧化指标的影响

在线全文

学校读者我要写书评

暂无评论

水产学报 2024年第6期48卷 194-204页

作者：周康奇韦孜娜李哲林勇黄姻杜雪松覃俊奇陈忠欧红霞徐俊龙项桂德潘贤辉广西壮族自治区水产科学研究院广西水产遗传育种与健康养殖重点实验室广西水产良种海南南繁基地广西钦州农业学校防城港职业技术学院

为改进中国圆田螺保存技术，促进螺蛳粉产业可持续发展。本研究利用初始体重为(21.71±2.70) g的健康中国圆田螺1 500只，探讨了低温(4°C)无水条件下0、14、28、42和56 d等不同时间中国圆田螺存活、形体指标、一般营养组成、脂肪酸、... 详细信息

为改进中国圆田螺保存技术，促进螺蛳粉产业可持续发展。本研究利用初始体重为(21.71±2.70) g的健康中国圆田螺1 500只，探讨了低温(4°C)无水条件下0、14、28、42和56 d等不同时间中国圆田螺存活、形体指标、一般营养组成、脂肪酸、消化酶活性和抗氧化指标的变化。结果显示，4°C短期存放(14～28 d)中国圆田螺的存活率均超过93%，但是超过42 d后，存活率降至78%以下。低温28和42 d的肝重和肝体比均显著高于对照组，与对照组相比，低温56 d体重降了7.6%，而肝重显著提高。营养成分结果显示，各组间肌肉的粗蛋白、粗灰分和水分均无显著影响。而粗脂肪含量呈现上升趋势，其中28、42和56 d的粗脂肪含量显著高于对照组。糖原含量总体呈上升趋势，其中14与56 d差异显著。脂肪酸结果显示，SFA和MUFA含量总体均呈下降趋势，28～56 d均显著低于对照和14 d组；28和42 d的PUFA含量显著高于其他处理组。本研究中所有消化酶和抗氧化酶活性总体均呈现下降趋势。对照组的淀粉酶活性显著低于14 d，但显著高于42 d。除42d的胰蛋白酶外，其余处理的脂肪酶和胰蛋白酶含量显著低于对照组。42 d的谷胱甘肽过氧化氢酶显著低于其他各组，且42 d的超氧化物歧化酶、过氧化氢酶活性和丙二醛含量也均显著低于对照组。研究表明，在4°C条件下无水保活超过42 d会导致中国圆田螺出现较高的死亡率，由于42 d后体内的消化酶和抗氧化酶活性降低，内环境平衡被打破，进而导致高的死亡率。胁迫期间中国圆田螺主要以蛋白质作为能量来源，其次是大量的饱和脂肪酸与单不饱和脂肪酸作为能量物质被消耗，而多不饱和脂肪酸被更好地保留下来。本研究为了解中国圆田螺在低温无水保活不同时间下的生理生化适应对策及改善其储存运输管理提供科学依据。

关键词：中国圆田螺低温时间营养组成脂肪酸酶活性抗氧化指标

Joint effects of microwave and chromium trioxide on root tip cells of Vicia faba

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第3期7卷 221-227页

作者： QIAN Xiao-wei LUO Wei-hua ZHENG ou-xiang School of Life and EnvironmentalScience Wenzhou University Wenzhou 325003 China

The mutagenic effects of microwave and chromium trioxide (CrO3) on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO3. The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO3, in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO3 concentration. We concluded that microwave and CrO3 had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells.

关键词：蚕豆三氧化铬微波对抗效应协同效应根尖细胞

饮水铅暴露对小鼠母代及其子代海马和皮层组织形态学的影响

在线全文

学校读者我要写书评

暂无评论

毒理学杂志 2024年第2期38卷 129-134,140页

作者：韦若昆韦佩祁袁海燕易湘姜岳明区仕燕李少军广西医科大学公共卫生学院卫生毒理学教研室广西环境与健康研究重点实验室高校高发疾病预防与控制研究重点实验室广西南宁530021

目的建立经饮水染铅小鼠模型,探讨孕期、哺乳期染铅对小鼠母代和子代海马及皮层组织形态学及母代小鼠的学习记忆能力的影响。方法30只成年雌性C57BL/6J小鼠按体重随机分为正常对照组(对照组)和低、高剂量染铅(低、高剂量)组。其中对照... 详细信息

目的建立经饮水染铅小鼠模型,探讨孕期、哺乳期染铅对小鼠母代和子代海马及皮层组织形态学及母代小鼠的学习记忆能力的影响。方法30只成年雌性C57BL/6J小鼠按体重随机分为正常对照组(对照组)和低、高剂量染铅(低、高剂量)组。其中对照组自由饮用超纯水,低、高剂量组分别饮用0.5和2 g/L醋酸铅1周后合笼繁殖仔鼠,并持续染毒至其子代4周龄。采用Morris水密宫检测母代小鼠的学习与记忆能力,苏木素-伊红(HE)染色观察母代小鼠及仔鼠的海马与前额皮层的组织形态学改变。结果与对照组比较,低和高剂量组母代小鼠逃避潜伏期及高剂量组母代小鼠游泳路程延长,而低和高剂量组母代小鼠穿台次数降低(P<0.05,χ^(2)=20.381)。对照母鼠海马各区及皮层细胞染色均匀,细胞膜清晰,胞核圆润,细胞形态结构相对完整且排列良好。低剂量组母代小鼠CA3区及仔鼠海马CA1、CA2、CA3及DG区出现不同程度的细胞核固缩、碎裂,细胞结构不清等组织病理学改变。高剂量染铅可致母代小鼠及其仔鼠CA1、CA2、CA3及DG区出现不同程度的细胞核固缩、深染、碎裂,细胞收缩,结构模糊,细胞排列杂乱等组织病理学改变。低、高剂量组母代小鼠及仔鼠前额皮层出现细胞核固缩、肿胀,核仁破碎,细胞结构模糊。其损伤的程度随着染毒剂量的升高而增加。结论饮水染铅可引起母代小鼠海马及皮层神经细胞损伤,进而损伤认知功能,同时生命早期染铅可致子代小鼠海马、皮层组织形态学发生异常改变,以致神经细胞受损。

关键词：铅小鼠海马病理形态学

Pulse signal detection in cognitive UWB system

在线全文

学校读者我要写书评

暂无评论

The Journal of China Universities of Posts and Telecommunications 2012年第3期19卷 74-79页

作者： SONG Xiao-ou xiang Xin BI Du-yan DENG Yong Engineering College Air Force Engineering.UniversityXi'an 710038China

According to the problem of cognitive ultra wide-band （UWB） spectrum sensing, a novel UWB pulse signal detection algorithm based on cumulative sum （CUSUM） test is proposed in this paper. Based on the analysis of the existing spectrum sensing schemes for cognitive UWB system, some obvious facts are obtained that it is difficult to detect UWB pulse signal with conventional spectrum sensing schemes, due to its low average signal to noise ratio （SNR）, large bandwidth, and low duty ratio. In this paper the detection algorithm of signal distribution change, which is application of CUSUM test, is considered to be applied to cognitive UWB spectrum sensing. But CUSUM test request that the pre-change and the post-change distributions are i.i.d, which cannot be satisfied in the detection process of UWB pulse signal. Since there are two time domain descriptions on UWB pulse signal, namely one contains only noise and the other one contains pulse signal plus noise, the existing detection algorithm of signal distribution change cannot be directly applied to detect UWB pulse signal. Hence the uniform probability density function expression of UWB pulse signal is first deduced, then CUSUM test is applied to cognitive UWB spectrum sensing. The proposed algorithm is a time sequential detection algorithm, with low complexity and minimal detection delay, which is suitable to detect the low duty ratio signal. Its performance is evaluated through theoretical analysis and numerical simulations. It is shown that this algorithm outperforms the conventional energy detection algorithm and conquers SNR wall phenomenon.

关键词： cognitive UWB （CUWB） spectrum sensing pulse signal CUSUM

Formation of multifaceted nano-groove structure on rutile TiO_(2) photoanode for efficient electron-hole separation and water splitting

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2022年第2期31卷 19-25页

作者： Xiaoyi Zhan Yaling Luo Ziyu Wang Yao xiang Zheng Peng Yong Han Hui Zhang Ruotian Chen Qin Zhou hongru Peng Hao Huang Weimin Liu Xin ou Guijun Ma Fengtao Fan Fan Yang Can Li Zhi Liu State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China School of Physical Science and Technology Shanghai Tech UniversityShanghai 201210China State Key Laboratory of Catalysis Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Photoelectrochemical(PEC)water-splitting using solar energy holds great promise for the renewable energy future,and a key challenge in the development of industry viable PEC devices is the unavailability of high-efficient photoanodes.Herein,we designed a TiO_(2) model photocatalyst with nano-groove pattern and different surface orientation using low-energy Ar+irradiation and photoetching of TiO_(2),and significantly improved the intrinsic activity for PEC water oxidation.High-resolution transmission electron microscopy directly manifests that the grooves consist of highly stepped surface withsteps and well-crystallized.Transient absorption spectroscopy reveals the groove surface that allows for increased recovery lifetime,which ensures promoted electron-hole separation efficiency.Surface photovoltage directly shows the carrier separation and transportation behaviors,verified by selective photodeposition,demonstrating the groove surface on TiO_(2) contributes to electron-hole separation.This work proposes an efficient and scalable photoanode strategy,which potentially can open new opportunities for achieving efficient PEC water oxidation performance.

关键词： TiO_(2)nano-grooves Oxygen vacancies Photoetching Water oxidation