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致敏小鼠骨髓间充质干细胞体外培养及多向分化功能的测定

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中国病理生理杂志 2010年第6期26卷 1203-1207页

作者：洪冬玲吴燕峰许吕宏方建培中山大学附属第二医院儿科广东广州510120 中山大学附属第二医院生物治疗中心广东广州510120

目的:评价异基因脾细胞输注致敏的小鼠骨髓源性间充质干细胞(MSCs)的体外培养生长能力及其多向分化功能。方法:应用贴壁培养法体外培养间充质干细胞,流式检测其表面标志以及检测其成骨、成脂和成肌多向分化状况;结果:致敏小鼠骨髓源性M... 详细信息

目的:评价异基因脾细胞输注致敏的小鼠骨髓源性间充质干细胞(MSCs)的体外培养生长能力及其多向分化功能。方法:应用贴壁培养法体外培养间充质干细胞,流式检测其表面标志以及检测其成骨、成脂和成肌多向分化状况;结果:致敏小鼠骨髓源性MSCs与非致敏小鼠骨髓源性MSCs比较,形态学无差异且均表达CD29+、CD105+、CD44+和Sca-1+;CD34-、CD11b-;同时在相应的诱导条件下具有向成骨、成脂、成肌多向分化的能力。结论:异基因脾细胞输注致敏的小鼠,其骨髓源性MSCs的形态学和功能与正常小鼠的MSCs比较评估未见异常。

关键词：致敏小鼠间充质干细胞细胞分化

骨髓间充质干细胞对致敏小鼠淋巴细胞增殖的影响及其作用方式

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中国实验血液学杂志 2011年第5期19卷 1209-1213页

作者：洪冬玲吴燕峰许吕宏方建培中山大学孙逸仙纪念医院儿科广东广州510120 广东省人民医院儿科中山大学孙逸仙纪念医院生物治疗中心广东广州510120

本研究探讨骨髓间充质干细胞(mesenchymal stem cells,MSC)在体外对致敏小鼠淋巴细胞增殖的影响及其作用方式,以便进一步了解MSC对致敏小鼠影响的可能机制。建立致敏模型后,将应用贴壁培养法体外培养的正常小鼠骨髓MSC或其培养上清液作... 详细信息

本研究探讨骨髓间充质干细胞(mesenchymal stem cells,MSC)在体外对致敏小鼠淋巴细胞增殖的影响及其作用方式,以便进一步了解MSC对致敏小鼠影响的可能机制。建立致敏模型后,将应用贴壁培养法体外培养的正常小鼠骨髓MSC或其培养上清液作为免疫细胞或免疫因子,致敏小鼠的淋巴细胞作为效应细胞,用植物血凝素(phytohemagglutinin,PHA)刺激淋巴细胞增殖,在体外应用MTT法检测两者的混合淋巴细胞增殖反应。结果表明,正常小鼠骨髓MSC在体外能很好地抑制致敏小鼠脾淋巴细胞增殖,同时在MSC培养上清液也能观察到类似现象,而且随着两者细胞比例或浓度比的增大,抑制作用有所加大,当两者比例为1∶1时抑制作用最大(有明显的统计学意义)。结论:MSC能在体外明显抑制致敏小鼠脾淋巴细胞增殖,该作用可以通过细胞与细胞(MSC和淋巴细胞)直接接触抑制,也可通过细胞与细胞的间接作用(如MSC的培养上清液与淋巴细胞)方式发挥作用。

关键词：骨髓间充质干细胞致敏小鼠混合淋巴细胞反应

Induced pluripotent stem cell-related approaches to generate dopaminergic neurons for Parkinson's disease

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Neural Regeneration Research 2025年第11期20卷 3193-3206页

作者： ling-Xiao Yi Hui Ren Woon Genevieve Saw Li Zeng Eng King Tan Zhi dong Zhou National Neuroscience Institute Singapore Nanyang Technology University Singapore Department of Neurology Singapore General HospitalSingapore Signature Research Program in Neuroscience and Behavioral Disorders Duke-NUS Medical SchoolSingapore

The progressive loss of dopaminergic neurons in affected patient brains is one of the pathological features of Parkinson's disease,the second most common human neurodegenerative *** the detailed pathogenesis accounting for dopaminergic neuron degeneration in Parkinson's disease is still unclear,the advancement of stem cell approaches has shown promise for Parkinson's disease research and *** induced pluripotent stem cells have been commonly used to generate dopaminergic neurons,which has provided valuable insights to improve our understanding of Parkinson's disease pathogenesis and contributed to anti-Parkinson's disease *** current review discusses the practical approaches and potential applications of induced pluripotent stem cell techniques for generating and differentiating dopaminergic neurons from induced pluripotent stem *** benefits of induced pluripotent stem cell-based research are *** dopaminergic neuron differentiation protocols from induced pluripotent stem cells are *** emerging three-dimension-based brain organoid models compared with conventional two-dimensional cell culture are ***,limitations,challenges,and future directions of induced pluripotent stem cell–based approaches are analyzed and proposed,which will be significant to the future application of induced pluripotent stem cell-related techniques for Parkinson's disease.

关键词： dopaminergic neurons induced pluripotent stem cells Parkinson's disease stem cell approaches

Low-temperature chlorination roasting technology for the simultaneous recovery of valuable metals from spent LiCoO_(2)cathode material

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 80-91页

作者： Junjie Shi Changle Hou Jingjing dong dong Chen Jianzhong Li Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

With the continuous increase in the disposal volume of spent lithium-ion batteries(LIBs),properly recycling spent LIBs has become essential for the advancement of the circular *** study presents a systematic analysis of the chlorination roasting kinetics and proposes a new two-step chlorination roasting process that integrates thermodynamics for the recycling of LIB cathode *** activation energy for the chloride reaction was 88.41 kJ/mol according to thermogravimetric analysis–derivative thermogravimetry data obtained by using model-free,model-fitting,and Z(α)function(αis conversion rate).Results indicated that the reaction was dominated by the first-order(F1)model when the conversion rate was less than or equal to 0.5 and shifted to the second-order(F2)model when the conversion rate exceeded *** conditions were determined by thoroughly investigating the effects of roasting temperature,roasting time,and the mass ratio of NH_(4)Cl to LiCoO_(2).Under the optimal conditions,namely 400℃,20 min,and NH_(4)Cl/LiCoO_(2)mass ratio of 3:1,the leaching efficiency of Li and Co reached 99.43% and 99.05%,*** of the roasted products revealed that valuable metals in LiCoO_(2)transformed into CoCl_(2) and ***,the reaction mechanism was elucidated,providing insights for the establishment of a novel low-temperature chlorination roasting technology based on a crystal structure *** technology can guide the development of LIB recycling processes with low energy consumption,low secondary pollution,high recovery efficiency,and high added value.

关键词： spent lithium-ion battery thermodynamics chlorination roasting kinetics circular economy

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FAK基因沉默诱导白血病细胞凋亡

中国病理生理杂志 2010年第7期26卷 1352-1355页

作者：许吕宏方建培 Le Yi 翁文骏洪冬玲中山大学孙逸仙纪念医院儿科广东广州510120 哈佛医学院波士顿儿童医院美国波士顿MA02115

目的:本研究靶向黏着斑激酶(FAK)基因,构建FAK-shRNA慢病毒载体,并研究FAK基因沉默对白血病细胞生长的影响。方法:化学合成人FAK基因的shRNA序列,应用分子生物学方法将该FAK shRNA序列插入含荧光素GFP慢病毒质粒。该FAK shRNA慢病毒载... 详细信息

目的:本研究靶向黏着斑激酶(FAK)基因,构建FAK-shRNA慢病毒载体,并研究FAK基因沉默对白血病细胞生长的影响。方法:化学合成人FAK基因的shRNA序列,应用分子生物学方法将该FAK shRNA序列插入含荧光素GFP慢病毒质粒。该FAK shRNA慢病毒载体在体外包装、浓缩,然后转染至人白血病细胞株。应用逆转录聚合酶链反应及蛋白质印迹方法检测FAK基因表达水平,予Annexin V标记检测细胞凋亡的情况。结果:经核苷酸序列分析提示FAK shRNA正确插入慢病毒质粒,该病毒载体在白血病细胞株的转染率为10%-25%。与对照组相比(无FAK shRNA的慢病毒载体),FAK shRNA慢病毒载体在细胞FAK mRNA及蛋白水平的抑制率分别为40%和70%。凋亡实验表明,对照组与FAK shRNA慢病毒载体组的Annexin V阳性率分别为(4.19±0.36)%和(8.48±0.58)%,两者差异明显(P<0.05)。结论:我们成功构建FAK shRNA慢病毒载体,该载体能抑制FAK基因表达并诱导白血病细胞凋亡。

关键词：黏着斑激酶基因沉默白血病细胞凋亡 REH细胞

Enhanced solid-electrolyte interface efficiency for practically viable hydrogen-air fuel cell systems

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Journal of Energy Chemistry 2025年第1期100卷 356-368页

作者： Venkitesan Sakthivel dong Jin Yoo Graduate School Department of Energy Storage/Conversion Engineering (BK21 FOUR) Hydrogen and Fuel Cell Research Center Jeonbuk National University Jeonju Department of Life Science Jeonbuk National University

Proton exchange membrane fuel cells （PEMFCs） provide an appealing sustainable energy system,with the solid-electrolyte membrane playing a crucial role in its overall ***,sulfonated poly（1,4-phenylene ether-ether sulfone）(SPEES),an aromatic hydrocarbon polymer,has garnered considerable attention as an alternative to Nafion ***,the long-term durability and stability of SPEES present a significant *** this context,we introduce a potential solution in the form of an additive,specifically a core–shell-based amine-functionalized iron titanate （A–Fe2TiO5）,which holds promise for improving the lifetime,proton conductivity,and power density of SPEES in *** modified SPEES/A–Fe2TiO5composite membranes exhibited notable characteristics,including high water uptake,enhanced thermomechanical stability,and oxidative ***,the SPEES membrane loaded with 1.2 wt%of A–Fe2TiO5demonstrates a maximum proton conductivity of 155 mS cm-1,a twofold increase compared to the SPEES membrane,at 80°C under 100%relative humidity （RH）.Furthermore,the 1.2 wt%of A–Fe2TiO5/SPEES composite membranes exhibited a maximum power density of 397.37 mW cm-2and a current density of 1148 mA cm-2at 60°C under 100%RH,with an opencircuit voltage decay of 0.05 m V/h during 103 h of continuous *** study offers significant insights into the development and understanding of innovative SPEES nanocomposite membranes for PEMFC applications.

关键词： Composite membranes

An NIR-Ⅱ-emissive Ru(Ⅱ) metallacycle for efficient and safe immunotherapy

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Science China Chemistry 2025年第1期68卷 5-7页

作者： Chonglu Li Jianbo Zhan Biao dong Institute of Health Inspection and Testing Hubei Provincial Center for Disease Control and Prevention State Key Laboratory on Integrated Optoelectronics College of Electronic Science and Engineering Jilin University

Immunotherapy, as a groundbreaking cancer treatment, has gained considerable attention for its utilization of the body’s immune system to combat tumors [1]. This therapy induces tumor cell apoptosis, triggering a sustained anti-tumor immune response and effectively preventing tumor recurrence and metastasis [2]. The fundamental principle lies in inducing tumor cell death through specific treatment modalities, leading to the release of a cascade of signaling molecules and antigens, thereby mobilizing the immune system to actively identify and attack tumor cells [3].

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抗CD20单抗在致敏受者造血干细胞移植中应用的实验研究

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中国病理生理杂志 2009年第10期25卷 2040-2043页

作者：许吕宏方建培 Le Yi 洪冬玲翁文骏魏菁中山大学孙逸仙纪念医院儿科广东广州510120 哈佛医学院波士顿儿童医院美国波士顿MA02115 中山大学孙逸仙纪念医院医学研究中心广东广州510120

目的:寻找促进异基因造血干细胞在致敏受者植入的策略研究,探讨抗CD20单抗在致敏受者造血干细胞移植的作用。方法:分别于移植前第14d及第7d予BALB/c小鼠输注C57BL/6小鼠的脾细胞建立致敏模型。实验组于移植前第11d经尾静脉输注抗CD20单... 详细信息

目的:寻找促进异基因造血干细胞在致敏受者植入的策略研究,探讨抗CD20单抗在致敏受者造血干细胞移植的作用。方法:分别于移植前第14d及第7d予BALB/c小鼠输注C57BL/6小鼠的脾细胞建立致敏模型。实验组于移植前第11d经尾静脉输注抗CD20单抗(美罗华)2mg/mouse,对照组于移植前第11d予输注RPMI-1640培养液0.2mL/mouse。于第0d(移植当天)取部分小鼠分离得血清及脾细胞,并检测供者反应性抗体及CD19+B细胞;部分小鼠予[^(60)Co]致死量照射,4h后予1×107C57BL/6小鼠骨髓细胞进行移植,观察生存情况及血常规恢复情况。结果:实验组与对照组血清细胞毒性指数分别为(37.00±3.46)%和(51.80±3.49)%,差异显著(P0.05)。濒死动物血常规结果示三系减少,提示受者死于造血衰竭。结论:抗CD20单抗能杀伤受者B细胞,降低致敏程度,但实验中该单抗并不能有效促进异基因造血干细胞在致敏受者的植入。

关键词：致敏受者抗CD20单抗造血干细胞移植

An Artificial Intelligence‑Assisted Flexible and Wearable Mechanoluminescent Strain Sensor System

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Nano-Micro Letters 2025年第3期17卷 217-231页

作者： Yan dong Wenzheng An Zihu Wang dongzhi Zhang College of Control Science and Engineering China University of Petroleum(East China)Qingdao 266580People’s Republic of China

The complex wiring,bulky data collection devices,and difficulty in fast and on-site data interpretation significantly limit the practical application of flexible strain sensors as wearable *** tackle these challenges,this work develops an artificial intelligenceassisted,wireless,flexible,and wearable mechanoluminescent strain sensor system(AIFWMLS)by integration of deep learning neural network-based color data processing system(CDPS)with a sandwich-structured flexible mechanoluminescent sensor(SFLC)*** SFLC film shows remarkable and robust mechanoluminescent performance with a simple structure for easy *** CDPS system can rapidly and accurately extract and interpret the color of the SFLC film to strain values with auto-correction of errors caused by the varying color temperature,which significantly improves the accuracy of the predicted strain.A smart glove mechanoluminescent sensor system demonstrates the great potential of the AIFWMLS system in human gesture ***,the versatile SFLC film can also serve as a encryption *** integration of deep learning neural network-based artificial intelligence and SFLC film provides a promising strategy to break the“color to strain value”bottleneck that hinders the practical application of flexible colorimetric strain sensors,which could promote the development of wearable and flexible strain sensors from laboratory research to consumer markets.

关键词： Mechanoluminescent Strain sensor Flexible Deep learning Wireless