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Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging

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Science China Life Sciences 2025年

作者： Zhen-Nan Pan Hao-lin Zhang Kun-Huan Zhang Jia-Qian ju Jing-Cai Liu Shao-Chen Sun College of Animal Science and Technology Nanjing Agricultural University Reproductive Medicine Centre The Affiliated Yantai Yuhuangding Hospital of Qingdao University

Mitochondrial Rho-GTPase 1 (MIRO1) is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis. In present study, we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging. We found that MIRO1 expressed in mouse and porcine oocytes, and its expression decreased in aged mice. MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes, which could be rescued by exogenous MIRO1supplementation. Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins, and MIRO1 regulated motor protein Dynein for microtubule-organizing centers (MTOCs) dynamics at germinal vesicle (GV) stage, which determined meiotic resumption. Furthermore, we found that MIRO1 regulated Aurora A and kinesin family member 11 (KIF11) for meiotic spindle assembly in oocytes. Besides, MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1 (DRP1),Parkin and lysosomal-associated membrane protein 2 (LAMP2) for mitochondrial dynamics and mitophagy during oocyte meiosis. Taken together, our results suggested that MIRO1 played pivotal roles in meiotic resumption, spindle assembly and mitochondrial function in mouse and porcine oocytes, and its insufficiency might contribute to the oocyte maturation defects during aging.

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Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： lin-Yan Huang Yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang ju-Yun Ma Peng-Peng Li Hai-Wei Pu Xin-Yian Guo Jian-Gang Shen Su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

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表面活性剂提高磺胺嘧啶片溶出度研究

河北大学学报（自然科学版） 2005年第5期25卷 498-502页

作者：庞秀言席改卿孙汉文鞠红林河北大学化学与环境科学学院河北保定071002 邯郸师范专科学校化学系河北邯郸056004

研究了不同质量分数的单甘酯(0.623%,1.278%,2.017%,2.12%)、吐温-80(0.378%,0.764%,1.046%,1.162%)和OP_10(0.306%,0.505%,1.059%,1.115%)对磺胺嘧啶片溶出情况的影响.结果表明在添加浓度范围内,单甘酯、吐温-80和OP_10对片剂的崩解和溶出均具有明显的加速作用;按药典要求,可以用于磺胺嘧啶片剂生产的吐温-80的质量分数在1.162%以上,而OP_10的质量分数在1.059%以上.

关键词：溶出度磺胺嘧啶单甘酯吐温-80 OP-10

Characterization of mutations in gyrA, gyrB and parC gene associated with Fluoroquinolones-resistant Mycoplasma gallisepticum selected in Vivo

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Characterization of mutations in gyrA, gyrB and parC gene as...

Altered 3D genome reorganization mediates precocious myeloid differentiation of aged hematopoietic stem cells in inflammation

中国畜牧兽医学会兽医药理毒理学分会第九次学术讨论会

作者： JIANG hong-Xia, lin ju-Chun, CHEN Ji-Rong, WANG Xiu-Mei, CHEN Zhang-Liu, ZENG Zhen-ling Guangdong Provincial Key laboratory for Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642

M. gallisepticum reference strain PG31 was purchased from the Institute of Animal Drug Control of China (Beijing, China). Seven clinical isolates of M. gallisepticum were derived from trachea swabs taken from chronic respiratory disease suspicious chickens and identified by serum agglutination test. The minimum inhibitory concentration (MIC) of enrofloxacin were determined by the standard method of two-fold dilution of antibiotics in test *** DNA of M.

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Science China Life Sciences 2025年

作者： Xinyu Cui Yu Dong Qiang Zhan Yixin Huang Qianshu Zhu Zihao Zhang Guang Yang Liping Wang Shijun Shen Jia Zhao Zhiyi lin Jiatong Sun Zhongqu Su Yihan Xiao Chuyu Zhang Yuwei Liang Lu Shen Lichen Ji Xuguang Zhang Jiqing Yin hong Wang Zhiyang Chen Zhenyu ju Cizhong Jiang Rongrong Le Shaorong Gao Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital Frontier Science Center for Stem Cells School of Life Sciences and Technology Tongji University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration of Ministry of Education Orthopaedic Department of Tongji Hospital School of Life Sciences and Technology Tongji University Key Laboratory of Regenerative Medicine of Ministry of Education Institute of Aging and Regenerative Medicine Department of Developmental & Regenerative Medicine College of Life Science and Technology Jinan University Shandong Key Laboratory of Animal Bioengineering and Disease Prevention College of Animal Science and Technology Shandong Agricultural University Department of Joint Surgery Shanghai East Hospital Tongji University School of Medicine Shanghai Institute of Nutrition and Health Chinese Academy of Sciences

Inflammation is a driving force of hematopoietic stem cells(HSCs) aging, causing irreversible exhaustion of functional HSCs. However, the underlying mechanism of HSCs erosion by inflammatory insult remains poorly understood. Here, we find that transient LPS exposure primes aged HSCs to undergo accelerated differentiation at the expense of self-renewal, leading to depletion of HSCs. Meanwhile, the central regulator nuclear factor kappa B(NF-κB) mediating functional impairment by inflammation insult induces differential transcriptional response in aged HSCs compared with young HSCs, with precocious activation of myeloid lineage genes. Altered compartmentalization and chromatin loop formation are associated with aging-related differential transcriptional response in HSCs upon lipopolysaccharide(LPS)stimulation. Mechanistically, enhancer and promoter regions of myeloid lineage genes in aged HSCs are more accessible and display more rapid and prominent CTCF occupancy upon LPS stimulation. Our study provides comprehensive resources for the three-dimensional(3D)genome structure of HSCs and sheds light into the ordered genome organization and the associated transcriptome signature underlying HSCs aging.

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Finite element modeling of consolidation process of Si C fiber-reinforced titanium matrix composites via matrix-coated fiber method

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Rare Metals 2015年第12期34卷 844-850页

作者： Xiang-hong Xu Yan-Qing Yang Xian Luo lin Qin ju-hong Lou Qing Sun Shazhou Polytechnic Institute of Technology State Key Laboratory of Solidification Processing Northwestern Polytechnical University

The consolidation process of SiCf/Ti-6Al-4V composites by matrix-coated fiber (MCF) method via hot pressing was investigated using finite element modeling (FEM). By analyzing the elastic–plastic contact deformation of the representative aligned coated fibers, the consolidation maps delineating the time–temperature–pressure relationship for full densification were constructed. Both the flow coefficient and the contact area coefficient used to describe the contact deformation were calculated according to the model. In addition, the effect of fiber content on matrix stress distribution was analyzed. The results show that fiber content is a significant factor that influences the densification process. Higher fiber content will lower the consolidation rate.

关键词： Titanium matrix composites Consolidation Finite element modeling Matrix-coated fiber

Semiclassical Calculations of Recurrence Spectra for Lithium Atoms in Parallel Electric and Magnetic Fields

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Chinese Physics Letters 2002年第1期19卷 29-31页

作者： lin Sheng-Lu Zhang Qiu-ju Zhao Ke Song Xiao-hong Zhang Yan-Hui Department of Physics Shandong Normal University Ji'nan 250014

By using the region-splitting consistent and iterative method, we calculate the recurrence spectra of lithium atoms in parallel strong external electric and magnetic fields, and obtain the novel resonance structure in the photoabsorption spectrum above the ionization threshold with a constant scaled electric field at = 0.036, and a scaled energy at ε = 0.58 and ε = 0.006, respectively. The results are compared with those of hydrogen obtained by using standard closed orbit theory. It is demonstrated that the core-scattered effects exhibited in combination recurrence play a great role.

关键词： Electric fields

Experimental study on the detonation process of a pulse detonation engine with ionized seeds

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Defence Technology（防务技术） 2020年第1期16卷 178-187页

作者： ling lin Shun-an Hu Yan-bin Hu Guang-ju Xu hong-yu Jiao Chun-sheng Weng School of Automotive Engineering Changshu Institute of TechnologySuzhou215500China National Key Laboratory of Transient Physics Nanjing University of Science and TechnologyNanjing210094China

An experimental platform of a pulse detonation engine(PDE) was established to study the effect of different K_2 CO_3 ionized seed mass contents on the detonation *** pressure and ion concentration were detected in the detonation process of the PDE with different contents of ionized *** initiation process of the PDE at different ignition frequencies was *** results show that the gas conductivity in the detonation process increased by adding ionized seeds to the PDE tube,and the conductivity increased with the increase in ionized seed mass *** the increase in ionized seed mass content,the range of the conductivity *** PDE was successfully ignited and formed a stable detonation wave at ignition frequencies of 5 Hz and 10 Hz,and the peak pressure of the stable detonation with the ignition frequency of 5 Hz was 17% higher than that with an ignition frequency of 10 *** detonation wave intensity was weake ned and dege nerated to a shock wave that propagated in the tube without the fuel filled at the ignition frequency of 20 Hz.

关键词： Pulse detonation engine Ionized seeds Detonation process Ignition frequency

Multiple factors influencing high-purity indium electrolytic refining

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Chinese Journal of Chemical Engineering 2024年第7期71卷 148-160页

作者： hong-Qiang Fan Fei Li hong-Xing Zheng Wu-ji Pan Mei-Zhen Wu Yashar Behnamian ju-Bo Peng Dong-Hai lin State Key Laboratory of Advanced Special Steel&School of Materials Science and Engineering Shanghai UniversityShanghai 200444China Shanghai Engineering Research Center for Integrated Circuits and Advanced Display Materials Shanghai UniversityShanghai 200444China Research&Development Center Yunnan Tin Group(Holding)Limited CompanyKunming 650032China Department of Chemical and Materials Engineering University of AlbertaEdmonton T6G 2V4AlbertaCanada School of Energy and Materials Shanghai Key Laboratory of Engineering Materials Application and EvaluationShanghai Polytechnic UniversityShanghai 201209China

The effects of various contaminants in the electrolytic refinement of indium were investigated using a glow discharge mass spectrometer(GDMS).The effects of several factors such as the indium ion(In3+)concentration,the sodium chloride(NaCl)concentration,the current density,the gelatin concentration,the pH,and the electrode distance,were *** variations in impurity levels concerning gelatin concentration were *** the gelatin and In3+concentration were moderately positively correlated with the Pb *** Sb concentration was associated positively with the NaCl concentration,while the Ti concentration had an adverse correlation with the NaCl *** Bi element content was positively linked to the electrode *** the current density increased,Cu,Pb,and Bi impurities initially rose and then eventually ***,a critical current density of 45 A·m^(-2) was identified in this behavior.

关键词： High-purity indium Electrolysis Multiple factors Electrochemistry Purification