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Melatonin protects liver from intestine ischemia reperfusion injury in rats

World Journal of Gastroenterology 2008年第48期14卷 7392-7396页

作者： Jian-Yi Li hong-zhuan yin Xi Gu Yong Zhou Wen-Hai Zhang Yi-Min Qin The Fifth Department of General Surgery Shengjing Affiliated Hospital of China Medical University Shenyang 110004 Liaoning Province China The Second Department of General Surgery Shengjing Affiliated Hospital of China Medical University Shenyang 110004 Liaoning Province China The Sixth Department of General Surgery Shengjing Affiliated Hospital of China Medical University Shenyang 110004 Liaoning Province China Department of General Surgery The First People's Hospital of Shenyang Shenyang 110004 Liaoning Province China

AIM: To investigate the protective effect of melatonin on liver after intestinal ischemia-reperfusion injury in rats. METHODS: One hundred and fifty male Wistar rats, weighing 190-210 g, aged 7 wk, were randomly divided into melatonin exposure group, alcohol solvent control group and normal saline control group. Rats in the melatonin exposure group received intraperitoneal (IP) melatonin (20 mg/kg) 30 min before intestinal ischemia-reperfusion (IR), rats in the alcohol solvent control group received the same concentration and volume of alcohol, and rats in the normal saline control group received the same volume of normal saline. Serum samples were collected from each group 0.5, 1, 6, 12, and 24 h after intestinal IR. Levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured with an auto-biochemical analyzer. Serum TNF-α was tested by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) in liver was detected by colorimetric assay. Pathological changes in liver and immunohistochemical straining of ICAM-1 were observed under an optical microscope. RESULTS: The levels of ALT measured at various time points after intestinal IR in the melatonin exposure group were significantly lower than those in the other two control groups (P < 0.05). The serum AST levels 12 and 24 h after intestinal IR and the ICAM-1 levels (%) 6, 12 and 24 h after intestinal IR in the melatonin exposure group were also significantly lower than those in the other two control groups (P < 0.05). CONCLUSION: Exotic melatonin can inhibit the activity of ALT, AST and TNF-α, decrease the accumulation of MDA, and depress the expression of ICAM-1 in liver after intestinal IR injury, thus improving the liver function.

关键词： Melatonin Intestinal ischemia-reperfusion injury Liver TNF-α

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Nutrition support in surgical patients with colorectal cancer

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World Journal of Gastroenterology 2011年第13期17卷 1779-1786页

作者： Yang Chen Bao-Lin Liu Bin Shang Ai-Shan Chen Shi-Qing Liu Wei Sun hong-zhuan yin Jian-Qiao yin Qi su Department of Surgery Shengjing Hospital China Medical UniversityShenyang 110004 Liaoning Province China

AIM: To review the application of nutrition support in patients after surgery for colorectal cancer, and to propose appropriate nutrition strategies. METHODS: A total of 202 consecutive surgical patients admitted to our hospital with a diagnosis of colon cancer or rectal cancer from January 2010 to July 2010, meeting the requirements of Nutrition Risk Screening 2002, were enrolled in our study. Laboratory tests were performed to analyze the nutrition status of each patient, and the clinical outcome variables, including postoperative complications, hospital stay, cost of hospitalization and postoperative outcome, were analyzed. RESULTS: The "non-risk" patients who did not receive postoperative nutrition support had a higher rate of postoperative complications than patients who received postoperative nutrition support (2.40 ± 1.51 vs 1.23 ± 0.60, P = 0.000), and had a longer postoperative hospital stay (23.00 ± 15.84 d vs 15.27 ± 5.89 d, P = 0.009). There was higher cost of hospitalization for patients who received preoperative total parenteral nutrition (TPN)than for patients who did not receive preoperative TPN (62 713.50 ± 5070.66 RMB Yuan vs 43178.00 ± 3596.68 RMB Yuan, P = 0.014). Applying postoperative enteral nutrition significantly shortened postoperative fasting time (5.16 ± 1.21 d vs 6.40 ± 1.84 d, P = 0.001) and postoperative hospital stay (11.92 ± 4.34 d vs 15.77 ± 6.03 d, P = 0.002). The patients who received postoperative TPN for no less than 7 d had increased serum glucose levels (7.59 ± 3.57 mmol/L vs 6.48 ± 1.32 mmol/L, P = 0.006) and cost of hospitalization (47 724.14 ± 16 945.17 Yuan vs 38 598.73 ± 8349.79 Yuan, P = 0.000). The patients who received postoperative omega-3 fatty acids had a higher rate of postoperative complications than the patients who did not (1.33 ± 0.64 vs 1.13 ± 0.49, P = 0.041). High level of serum glucose was associated with a high risk of postoperative complications of infection. CONCLUSION: Appropriate and moderate nutrition

关键词： Nutritional support Nutrition assessment Colorectal cancer Surgery Prognosis

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侧方淋巴结清扫在国内低位直肠癌治疗中应用的Meta分析

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世界华人消化杂志 2016年第21期24卷 3270-3280页

作者：朱明达殷红专苏琪中国医科大学附属盛京医院结直肠肛门病外科辽宁省沈阳市110004

目的:评价侧方淋巴结清扫在国内低位直肠癌治疗中应用价值.方法:运用M e t a分析的方法对我国2005-01/2015-09公开发表的有关侧方淋巴结清扫在低位直肠癌中应用的11篇文献资料进行综合分析.结果:侧方清扫组手术时间明显长于未清扫组,合... 详细信息

目的:评价侧方淋巴结清扫在国内低位直肠癌治疗中应用价值.方法:运用M e t a分析的方法对我国2005-01/2015-09公开发表的有关侧方淋巴结清扫在低位直肠癌中应用的11篇文献资料进行综合分析.结果:侧方清扫组手术时间明显长于未清扫组,合并加权均数差(weighted mean d i ff e r e n c e,W M D)为47.79 m i n,且差异有统计学意义(P<0.00001).侧方清扫组手术失血量高于未清扫组,合并加权均数差为27.84 m L,且差异有统计学意义(P<0.0001).侧方淋巴结清扫组5年生存率高于未清扫组(59.8%vs 51.3%),差异具有统计学意义P<0.05(P=0.02).侧方淋巴结清扫组三年生存率明显高于未清扫组(81.6%vs63.5%),差异具有统计学意义(P<0.00001).侧方淋巴结清扫组的局部复发率明显低于未清扫组(8.4%vs 16.9%),差异具有统计学意义(P=0.0003).Ⅰ期及ⅡA期行侧方淋巴结清扫对于局部复发的影响差异无统计学意义(PⅠ期=0.96,PⅡA期=0.05).Ⅲ期低位直肠癌侧方淋巴结清扫组局部复发率明显低于未清扫组(14.5%vs 22.1%),P<0.05(P=0.01)差异具有统计学意义.结论:我国低位直肠癌患者行侧方淋巴结清扫能有效的延长患者的5年生存率及3年生存率,降低局部复发率(尤其是Ⅲ期低位直肠癌),改善患者预后,但会增加手术时间及术中出血量.

关键词：直肠癌侧方淋巴结复发率生存率

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超声刀联合弧形切割吻合器在低位直肠癌超低前切除术中的应用

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世界华人消化杂志 2009年第22期17卷 2326-2328页

作者：李英苏琪殷红专张光辉中国医科大学附属盛京医院第一微创外科胆道外科辽宁省沈阳市110004

目的:探讨超声刀联合弧形切割吻合器在低位直肠癌超低前切除术中的应用效果.方法:回顾性分析我院2005-07/2009-02收治的行直肠癌超低前切除术32例的临床资料.其中,采用超声刀联合弧形切割吻合器12例,采用电刀联合线形吻合器20例.观察2... 详细信息

目的:探讨超声刀联合弧形切割吻合器在低位直肠癌超低前切除术中的应用效果.方法:回顾性分析我院2005-07/2009-02收治的行直肠癌超低前切除术32例的临床资料.其中,采用超声刀联合弧形切割吻合器12例,采用电刀联合线形吻合器20例.观察2组患者的手术成功率、手术时间、术中出血量、术后前3d引流量、术后恢复时间以及术后并发症的差异.结果:超声刀联合弧形切割吻合器组较电刀联合线形吻合器组手术成功率显著提高(P<0.05),而手术时间(U=12.5,W=90.5,P<0.05)、术中出血量(t=-4.968,P<0.05)、术后前3d引流量(t=-6.507,P<0.05)、引流时间(t=-3.888,P<0.05)和恢复时间(U=27.0,W=105.0,P<0.05)方面均显著减少.结论:超声刀联合弧形切割吻合器在直肠癌超低前切除术中应用效果显著.

关键词：直肠癌超低前切除术超声刀弧形切割吻合器手术效果

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荧光定量PCR检测CK20在直肠癌外周血中表达的意义

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世界华人消化杂志 2005年第24期13卷 2892-2895页

作者：殷红专韩霞苏琪吴硕东刘恩卿中国医科大学附属盛京医院普外科辽宁省沈阳市110004

目的:分析外周血中CK20基因产物的表达与直肠癌Dukes分期和组织学分型的关系,探讨用FQ-PCR检测CK20来预测直肠癌微转移以及判断患者预后的价值.方法:采集47例直肠癌患者外周血标本、10例良性肠道病患者及10例健康志愿者外周血标本.用FQ-... 详细信息

目的:分析外周血中CK20基因产物的表达与直肠癌Dukes分期和组织学分型的关系,探讨用FQ-PCR检测CK20来预测直肠癌微转移以及判断患者预后的价值.方法:采集47例直肠癌患者外周血标本、10例良性肠道病患者及10例健康志愿者外周血标本.用FQ-PCR检测外周血CK20的表达情况.结果:10例良性肠道病患者及10例健康志愿者外周血中均检测不到CK20的表达,与直肠癌组有明显差异(72.34%,34/47)(χ2=37.434,P<0.01).47例直肠癌患者外周血中CK20的表达量与Dukes分期呈正相关(r=0.680,P<0.01).结论:直肠癌外周血中CK20的表达量与肿瘤分期有关,并且分期越晚CK20的表达量越多,用CK20FQ-PCR检测癌转移较常规病理组织学敏感,对直肠癌患者病情的判定、疗效评价及恢复情况具有重要临床意义.

关键词：荧光定量PCR 直肠癌 CK20

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Protective effect of ischemic preconditioning on hepatic ischemia-reperfusion injury by advancing the expressive phase of survivin in rats

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Hepatobiliary & Pancreatic Diseases International 2008年第6期7卷 615-620页

作者： Li, Jian-Yi Gu, Xi yin, hong-zhuan Zhou, Yong Zhang, Wen-Hai Qin, Yi-Min China Med Univ Shengjing Hosp Dept Gen Surg Shenyang 110004 Peoples R China Shenyang First Peoples Hosp Dept Gen Surg Shenyang 110044 Peoples R China

BACKGROUND: Survivin is a new and important gene in the regulation of apoptosis. It is very important to explore the effect of the expression of survivin protein caused by ischemia-reperfusion (IR) injury. The effect of IR injury caused by ischemic preconditioning (IP) on the liver in rats and the relation between the protective effect of IP and the expression of survivin are unclear. METHODS: One hundred and fifty male Wistar rats (weighing 190-210 g, aged 6-7 weeks) were divided into three groups at random: ischemic preconditioning (IP), ischemia-reperfusion (IR) and sham-operation (SO). Sample specimens were collected from each group at 6, 12, 24, 48, and 72 hours after reperfusion. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by an automatic biochemical analyzer. Malondialdehyde (MDA) in liver tissue was measured. Pathological changes in the liver and immunohistochemical staining for survivin were determined with an optical microscope. RESULTS: The ALT levels in the IP and IR groups after reperfusion at each time were higher than those in the SO group (P<0.05), whereas after reperfusion for 6 and 12 hours, the ALT levels in the IP group were lower than those in the IR group (P<0.05). The AST levels in all IP and IR groups were higher than those in the SO group (P<0.05), whereas after reperfusion for 12, 24, 48 and 72 hours, the AST levels in the IP group were lower than those in the IR group (P<0.05). The MDA concentrations after reperfusion in the IP group were lower than those in the IR group (P<0.05), though the MDA concentrations in the IP and IR groups increased in contrast to those in the SO group after reperfusion at each time (P<0.05). After reperfusion for 12, 24, 48 and 72 hours, the number of survivin-positive cells was larger in the IP and IR groups than in the SO group (P<0.05). After reperfusion for 12, 24, and 48 hours the number of survivin-positive cells in the IP group increased compared with that in t

关键词： ischemia-reperfusion injury ischemic preconditioning survivin protein

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SYNTHESES OF TETRAHEDRAL METAL CLUSTER COMPOUNDS

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Chinese Chemical Letters 1992年第7期3卷 551-554页

作者： Yuan Qi yin hong Sui SUN zhuan Yun ZHAO Lanzhou Institute of Chemical Physics Chinese Academy of SciencesLanzhou 730000 Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032

The carbyne compound [Br(CO)_2(Py)_2Mo(≡CC_6H_5)] (Py=pyridine) (1a) reacts with Co_2 (CO)_8, Fe_2(CO)_9 and Mn_2 (CO)_(10) to give tetrahedral tri-metal cluster compounds Co_2Mo(μ_3-CC_6H_5)Br(CO)_8(Py)_2 (2), Fe_2Mo(μ_3-CC_6H_5) Br(CO)_9(Py)_2 (3) and Mn_2Mo(μ_3-CC_6H_5)Br(CO)_(10) (Py)_2 (4) respectively. Tri-metal cluster compound Co_2Mo(μ_3-CC_6H_5)Br(CO)_8-(bipy) (bipy=α,α'dipyridyl) (5) is prepared in a similar reaction sequence from [Br(CO)_2(bipy)Mo(≡CC_6H_5)] (1b) and Co_2(CO)_8. IR, ~1H and ^(13)C NMR spectral data of these compounds are reported and discussed. The crystal structure of compound (5) has been determined by X-ray diffraction.

关键词： CO Br Hs SYNTHESES OF TETRAHEDRAL METAL CLUSTER COMPOUNDS Co Ho

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2-D/2-D heterostructured biomimetic enzyme by interfacial assembling Mn_(3)(PO_(4))_(2) and MXene as a flexible platform for realtime sensitive sensing cell superoxide

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Nano Research 2021年第3期14卷 879-886页

作者： Shen Fei Zhao Fang Xin Hu zhuan zhuan Shi Jing Jing Fu Yue Chen Fang yin Dai Chun Xian Guo Chang Ming Li Institute of Materials Science and Devices Suzhou University of Science and TechnologySuzhou215009China Institute for Clean Energy and Advanced Materials Southwest UniversityChongqing400715China State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing400715China Institute for Advanced Cross-field Science&College of Life Science Qingdao UniversityQingdao200671China

It is critical for fabricating flexible biosensors with both high sensitivity and good selectivity to realize real-time monitoring superoxide anion(O_(2)^(·−)),a specific reactive oxygen species that plays critical roles in various biological *** work delicately designs a Mn_(3)(PO_(4))_(2)/MXene heterostructured biomimetic enzyme by assembling two-dimensional(2-D)Mn_(3)(PO_(4))_(2) nanosheets with biomimetic activity and 2-D MXene nanosheets with high conductivity and abundant functional *** 2-D nature of the two components with strong interfacial interaction synergistically enables the heterostructure an excellent flexibility with retained 100%of the response when to reach a bending angle up to 180°,and 96%of the response after 100 bending/relaxing *** is found that the surface charge state of the heterostructure promotes the adsorption of O_(2)^(·−),while the high-energy active site improves electrochemical oxidation of O_(2)^(·−).The Mn_(3)(PO_(4))_(2)/MXene as a sensing platform towards O2•−achieves a high sensitivity of 64.93µA·µM^(−1)·cm^(−2),a wide detection range of 5.75 nM to 25.93µM,and a low detection limit of 1.63 ***,the flexible heterostructured sensing platform realizes real-time monitoring of O_(2)^(·−)in live cell assays,offering a promising flexible biosensor towards exploring various biological processes.

关键词： two-dimensional(2-D)nanosheets assembled heterostructure interface flexible biosensor real-time monitoring

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Impact of mTOR gene polymorphisms and gene-tea interaction on susceptibility to tuberculosis

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World Journal of Clinical Cases 2020年第19期8卷 4320-4330页

作者： Mian Wang Shu-Juan Ma Xin-yin Wu Xian Zhang Julius Abesig Zheng-Hui Xiao Xin Huang Hai-Peng Yan Jing Wang Meng-Shi Chen hong-zhuan Tan Hunan Provincial Key Laboratory of Clinical Epidemiology Xiangya School of Public HealthCentral South UniversityChangsha 410078Hunan ProvinceChina Department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangsha 410078Hunan ProvinceChina Department of Occupational and Environmental Hygiene Xiangya School of Public HealthCentral South UniversityChangsha 410078Hunan ProvinceChina Hunan Provincial Key Laboratory of Pediatric Emergency Hunan Children's HospitalChangsha 410007Hunan ProvinceChina Department of Epidemiology and Health Statistics Hunan Normal UniversityChangsha 410008Hunan ProvinceChina

BACKGROUND mTOR gene is a key component of the PI3K/Akt/mTOR signaling pathway,and its dysregulation is associated with various *** studies have demonstrated that tea drinking is a protective factor against tuberculosis(TB).This study was designed to explore five single nucleotide polymorphisms(SNPs)of mTOR in the Han population of China to determine how their interactions with tea drinking affect susceptibility to *** To investigate if the polymorphisms of mTOR gene and the gene-tea interaction are associated with susceptibility to *** In this case-control study,503 patients with TB and 494 healthy controls were enrolled by a stratified sampling *** cases were newly registered TB patients from the county-level centers for disease control and prevention,and the healthy controls were permanent residents from Xin'ansi Community,Changsha *** data and environmental exposure information including tea drinking were obtained from the study *** genotyped five potentially functional SNP sites(rs2295080,rs2024627,rs1057079,rs12137958,and rs7525957)of mTOR gene and assessed their associations with the risk of TB using logistic regression analysis,and marginal structural linear odds models were used to estimate the gene-environment *** The frequencies of four SNPs(rs2295080,rs2024627,rs1057079,and rs7525957)were found to be associated with susceptibility to TB(P<0.05).Genotypes GT(OR 1.334),GG(OR 2.224),and GT+GG(OR 1.403)at rs2295080;genotypes CT(OR 1.562)and CT+TT(OR 1.578)at rs2024627,genotypes CT(OR 1.597),CC(OR 2.858),and CT+CC(OR 1.682)at rs1057079;and genotypes CT(OR 1.559)and CT+CC(OR 1.568)at rs7525957 of mTOR gene were significantly more prevalent in TB patients than in healthy *** relative excess risk of interaction between the four SNPs(rs2295080,rs2024627,rs1057079,and rs7525957)of mTOR genes and tea drinking were found to be-1.5187(95%CI:-1.9826,-1.0547,P<0.05),-1.8270(95%CI:-2.3587,-

关键词： Tuberculosis mTOR Tea drinking Gene-environment interaction The relative excess risk of interaction Single nucleotide polymorphism

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Transforming growth factor-β1 in invasion and metastasis in colorectal cancer

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World Journal of Gastroenterology 2002年第4期8卷 674-678页

作者： Bin Xiong hong-yin Yuan Ming-Bo Hu Feng Zhang Zheng-zhuan Wei Ling-Ling Gong Guo-Liang Yang Department of Oncology,Affiliated Zhongnan Hospital of Wuhan University,Wuhan 430071,Hubei Province,China Department of Oncology Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430071Hubei ProvinceChina. xbxh@***

AIM：To investigate the role of TGFβ1 in invasion and metastasis in colorectal cancer by analysing TGFβ1 correlated wity depth of tumor invasion,stage and ***:Serum TGFβ1levels were determined in50patients with colorectal cancer and 30healthy volunteers using a TGFβ1 enzyme-linked immunosorbent ***β1 expression in primary and lymph node metastatic lesions were detected in 98cases of colorectal cancer by immunohistochemical staining and in situ ***:Serum levels of TGFβ1 in patients with colorectal cancer(40±18μg·L^-1)were significantly higher than those in the healthy control group(19±8μg·L^-1),P<*** levels of serum TGFβ1were found in 60%of patients with colorectal cancer when the mean+2s was used as the upper limit of the normal range(35.1μg·L^-1).Increases in serum TGFβ1 levels were significantly asociated with Dukei's stage(P<0.05),but there was no significant difference between,Duki's stage Bpatients and Dukei's stage *** the cytoplasm of cancer cells,TGFβ1 was immunostained in37.8%(37/98)of colorectal cancer,and this expression was confirmed by in situ hybridization,Among35cases of colorectal cancer with lymph node metastatic lesions,TGFβ1 positive staining was found in18(51.4%)cases of primary tumor,and 25(71.4%)cases with lymph node metastatic lesions,respectively,Of17cases with no staining in the primary lesion.7(41.2%)casesshowed TGFβ1 staining in the metastatic *** TGFβ1 levels and TGFβ1 expression in colorectal cancer tissues were correlated significantly with depth of tumor invasion,stage and metastasis,Patients in stage C-D,T3-T4and with metastasis had significantly higher TGFβ1 levels than patients in stage A-B,T1-T2and without metastasis(P<0.05).CONCLUSION:These results suggest that transforming growth factor-β1 is closely related to the invasion and metastasis of colorectal *** increased the invasive and metastatic potential of tumor by altering a tumor ***β1 ma

关键词：结直肠癌转化生长因子β1 肿瘤侵袭肿瘤转移作用机制

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