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Chromosome-level genome assembly of the glass catfish(Kryptopterus vitreolus)reveals molecular clues to its transparent phenotype

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Zoological Research 2024年第5期45卷 1027-1036页

作者： Chao Bian Rui-Han Li Zhi-Qiang Ruan Wei-Ting Chen yu Huang Li-yue Liu hong-Ling Zhou Cheong-Meng Chong Xi-Dong Mu Qiong shi Laboratory of Aquatic Genomics College of Life Sciences and OceanographyShenzhen UniversityShenzhenGuangdong 518057China Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species Ministry of Agriculture and Rural AffairsGuangdong Modern.Recreational Fisheries Engineering Technology CenterPearl River Fisheries Research InstituteChinese Academy of Fishery SciencesGuangzhouGuangdong 510380China Shenzhen Key Lab of Marine Genomics Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic AnimalsBGI Academy of Marine SciencesBGI MarineShenzhenGuangdong 518081China CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengduSichuan 610041China School of Life Sciences Jiaying UniversityMeizhouGuangdong 514015China China Zebrafish Resource Center National Aquatic Biological Resource CenterState Key Laboratory of Freshwater Ecology and BiotechnologyInstitute of HydrobiologyChinese Academy of ScienceWuhanHubei 430072China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518116China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacaoMacao 999078China Center for Aquatic Genomics College of Life SciencesNeijiang Normal UniversityNeijiangSichuan 641100China

Glass catfish(Kryptopterus vitreolus)are notable in the aquarium trade for their highly transparent body *** transparency is due to the loss of most reflective iridophores and light-absorbing melanophores in the main body,although certain black and silver pigments remain in the face and *** date,however,the molecular mechanisms underlying this transparent phenotype remain largely *** explore the genetic basis of this transparency,we constructed a chromosome-level haplotypic genome assembly for the glass catfish,encompassing 32 chromosomes and 23344 protein-coding genes,using PacBio and Hi-C sequencing technologies and standard assembly and annotation *** revealed a premature stop codon in the putative albinism-related tyrp1b gene,encoding tyrosinase-related protein 1,rendering it a nonfunctional ***,a synteny comparison with over 30 other fish species identified the loss of the endothelin-3(edn3b)gene in the glass catfish *** investigate the role of edn3b,we generated edn3b^(−/−)mutant zebrafish,which exhibited a remarkable reduction in black pigments in body surface stripes compared to wild-type *** findings indicate that edn3b loss contributes to the transparent phenotype of the glass *** high-quality chromosome-scale genome assembly and identification of key genes provide important molecular insights into the transparent phenotype of glass *** findings not only enhance our understanding of the molecular mechanisms underlying transparency in glass catfish,but also offer a valuable genetic resource for further research on pigmentation in various animal species.

关键词： Glass catfish Whole-genome sequencing edn3b−/−mutant Transparent phenotype

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Protective Graphite Coating for Two-Dimensional Carbon/Carbon Composites

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Fluid Dynamics & Materials Processing 2024年第1期20卷 97-108页

作者： Wei shi Zhengyi Li Xiaobing Xu Yingshui yu Xiaofei Ding Heng Ju School of Mechanical and Power Engineering Dalian Ocean UniversityDalian116023China School of Mechanical and Electrical Engineering Gongqing Institute of Science and TechnologyJiujiang332020China

Two-dimensional carbon/carbon(2D C/C)composites are a special class of carbon/carbon composites,generally obtained by combining resin-impregnated carbon fiber clothes,which are then cured and *** study deals with the preparation of a protective coating for these *** coating,based on graphite,was prepared by the slurry *** effect of graphite and phenolic resin powders with different weight ratios was *** results have shown that the coating slurry can fill the pores and cracks of the composite surface,thereby densifying the surface layer of the *** the increase of the graphite powder/phenolic resin weight ratio,the coating density is enhanced while the coating surface flatness decreases;moreover,the protective ability of coating against erosion first increases(from 1:3 to 2:2)and then decreases(from 2:2 to 3:1).When the weight ratio is about 1:1,the coating for 2D C/C composites exhibits the best erosion resistance,which greatly aids these materials during gas *** this case,the erosion rate is decreased by approximately 41.5%at the impact angle of 30°and 52.3%at normal impact,*** can be attributed to the ability of the coating slurry to infiltrate into the substrate,thereby bonding the fibers together and increasing the compactness of the 2D C/C composites.

关键词： Carbon/carbon composites graphite coating microstructure erosion resistance

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Preparation of Environmentally Friendly Urea-Hexanediamine-Glyoxal(HUG)Resin Wood Adhesive

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Journal of Renewable Materials 2024年第2期12卷 235-244页

作者： Qianyu Zhang shi Chen Long Cao hong Lei Antonio Pizzi Xuedong Xi Guanben Du yunnan Key Laboratory of Wood Adhesives and Glue Products College of Material Science and EngineeringSouthwest Forestry UniversityKunming650224China International Joint Research Center for Biomass Materials Southwest Forestry UniversityKunming650224China College of Chemical and Materials Engineering Zhejiang A&F UniversityHangzhou311300China LERMAB University of LorraineEpinal88000France

Using non-toxic,low-volatile glyoxal to completely replace formaldehyde for preparing urea-glyoxal(UG)resin adhesive is a hot research topic that could be of great interest for the wood ***,urea-glyoxal(UG)resins prepared by just using glyoxal instead of formaldehyde usually yields a lower degree of *** results in a poorer bonding performance and water resistance of UG resins.A good solution is to pre-react urea to preform polyurea molecules presenting already a certain degree of polymerization,and then to condense these with glyoxal to obtain a novel UG ***,in this present work,the urea was reacted with hexamethylene diamine to form a polyurea named HU,and then this was used to react it with different amounts of glyoxal to synthesize hexamethylenediamine-urea-glyoxal(HUG)polycondensation resins,and to use this for bonding *** results show that the glyoxal can well react with HU polyuria via addition and schiff base reaction,and also the HUG resin exhibits excellent bonding strength and water *** shear strength of the plywood bonded with this HUG at 160°C hot press temperature as high as 1.93 MPa,2.16 MPa and 1.61 MPa,respectively,which meets the requirement of the China national standard GB/T 9846-2015(≥0.7 MPa),and can be a good choice as a wood adhesive for industrial application.

关键词： Urea-glyoxal resin hexamethylene diamine wood adhesives water resistance

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Infrared Fault Detection Method for Dense Electrolytic Bath Polar Plate Based on YOLOv5s

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Computers, Materials & Continua 2024年第9期80卷 4859-4874页

作者： Huiling yu Yanqiu Hang Shen shi Kangning Wu Yizhuo Zhang Software Engineering Department of Computer ScienceChangzhou UniversityChangzhou213146China Electrical Engineering Department of Computer ScienceChangzhou UniversityChangzhou213146China

Electrolysis tanks are used to smeltmetals based on electrochemical principles,and the short-circuiting of the pole plates in the tanks in the production process will lead to high temperatures,thus affecting normal *** at the problems of time-consuming and poor accuracy of existing infrared methods for high-temperature detection of dense pole plates in electrolysis tanks,an infrared dense pole plate anomalous target detection network YOLOv5-RMF based on You Only Look Once version 5(YOLOv5)is ***,we modified the Real-Time Enhanced Super-Resolution Generative Adversarial Network(Real-ESRGAN)by changing the U-shaped network(U-Net)to Attention U-Net,to preprocess the images;secondly,we propose a new Focus module that introduces the Marr operator,which can provide more boundary information for the network;again,because Complete Intersection over Union(CIOU)cannot accommodate target borders that are increasing and decreasing,replace CIOU with Extended Intersection over Union(EIOU),while the loss function is changed to Focal and Efficient IOU(Focal-EIOU)due to the different difficulty of sample *** the homemade dataset,the precision of our method is 94%,the recall is 70.8%,and the map@.5 is 83.6%,which is an improvement of 1.3%in precision,9.7%in recall,and 7%in map@.5 over the original *** algorithm can meet the needs of electrolysis tank pole plate abnormal temperature detection,which can lay a technical foundation for improving production efficiency and reducing production waste.

关键词： Infrared polar plate fault detection YOLOv5 Real-ESRGAN Marr boundary detection operator Focal-EIoU loss

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Traceable ring signature schemes based on SM2 digital signature algorithm and its applications in the data sharing scheme

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Frontiers of Computer Science 2024年第2期18卷 251-253页

作者： Yongxin ZHANG hong LEI Bin WANG Qinghao WANG Ning LU Wenbo shi Bangdao CHEN Qiuling yuE R&D Department SSC Holding CompanyLtd.Chengmai 571924China School of Cyberspace Security(School of Cryptography) Hainan UniversityHaikou 570228China College of Computer Science and Engineering Northeastern UniversityShenyang 110819China School of Computer Science and Technology Xidian UniversityXi'an 710071China Blockchain Research Group Oxford-Hainan Blockchain Research InstituteChengmai 571924China

1 Introduction Identity privacy concerns hinder data sharing by casting doubt on the safeguarding of personal information,eroding trust,and impeding the willingness of individuals and organizations to exchange their data[1,2].The traceable ring signatures(TRSs)addresses the contradiction between identity privacy and regulation[3],no scheme has been developed thus far that is based on SM2,the Chinese cryptographic public key algorithm standard,without relying on centralized trust.

关键词： SM2 signature scheme

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Late-onset Cholestasis with Paucity of Portal Area Secondary 􀁗􀁒􀀃􀀫􀀱􀀩􀀔􀈕􀀃􀀧􀁈􀁉􀁌􀁆􀁌􀁈􀁑􀁆􀁜􀀃􀁌􀁑􀀃􀀤􀁇􀁘􀁏􀁗􀁋􀁒􀁒􀁇􀀝􀀃􀀤􀀃􀀦􀁄􀁖􀁈􀀃􀀵􀁈􀁓􀁒􀁕􀁗

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Journal of Clinical and Translational Hepatology 2024年第3期12卷 327-331页

作者： Xuemei Zhang Kun Liu Xiaona Lu Wenlan Zheng Jia shi shihan yu Hai Feng Zhuo yu Department of Hepatopathy Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiChina Department of Pathology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiChina Institute of Infectious Disease Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiChina

Hepatocyte nuclear factor 1β(HNF1β)is essential for biliary development,while its genetic defect triggers the dysplasia of interlobular bile ducts,leading to life-threatening hepatitis and *** date,this disorder has mainly been docu-mented in ***,we report a case of cholestasis in an adult patient caused by a de novo HNF1β mutation.A liver biopsy revealed remarkable shrinkage of the portal area accompanied by a decrease or absence of interlobular bile ducts,veins,and arteries in the portal *** case showed that an HNF1β defect could induce late-onset cholestasis with paucity of the portal area in adulthood.

关键词： HNF1β Cholestasis Adult patient Paucity of the portal area Renal cysts Case report

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Strong light–matter interactions based on excitons and the abnormal all-dielectric anapole mode with both large field enhancement and low loss

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Photonics Research 2024年第4期12卷 854-864页

作者： YAN-HUI DENG yu-WEI LU HOU-JIAO ZHANG Zhong-hong shi ZHANG-KAI ZHOU XUE-HUA WANG State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-sen UniversityGuangzhou 510275China Quantum Science Center of Guangdong–hong Kong–Macao Greater Bay Area(Guangdong) Shenzhen 518045China

The room temperature strong coupling between the photonic modes of micro/nanocavities and quantum emitters(QEs) can bring about promising advantages for fundamental and applied *** the electric fields(EFs) by using plasmonic modes and reducing their losses by applying dielectric nanocavities are widely employed approaches to achieve room temperature strong ***,ideal photonic modes with both large EFs and low loss have been ***,we propose the abnormal anapole mode (AAM),showing both a strong EF enhancement of~70-fold (comparable to plasmonic modes) and a low loss of 34 meV,which is much smaller than previous records of isolated all-dielectric *** realizing strong coupling,we further show that by replacing the normal anapole mode with the AAM,the lasing threshold of the AAM-coupled QEs can be reduced by one order of magnitude,implying a vital step toward on-chip integration of nanophotonic devices.

关键词： dielectric mode coupling

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Outage Probability Analysis for D2D-Enabled Heterogeneous Cellular Networks with Exclusion Zone:A Stochastic Geometry Approach

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工程与科学中的计算机建模(英文) 2024年第1期138卷 639-661页

作者： yulei Wang Li Feng Shumin Yao hong Liang Haoxu shi yuqiang Chen School of Computer Science and Engineering Macao University of Science and TechnologyTaipaMacaoChina Department of Broadband Communication Peng Cheng LaboratoryShenzhenChina School of Artificial Intelligence Dongguan PolytechnicDongguanChina

Interference management is one of the most important issues in the device-to-device(D2D)-enabled heterogeneous cellular networks(HetCNets)due to the coexistence of massive cellular and D2D devices in which D2D devices reuse the cellular *** alleviate the interference,an efficient interference management way is to set exclusion zones around the cellular *** this paper,we adopt a stochastic geometry approach to analyze the outage probabilities of cellular and D2D users in the D2D-enabled *** main difficulties contain three aspects:1)how to model the location randomness of base stations,cellular and D2D users in practical networks;2)how to capture the randomness and interrelation of cellular and D2D transmissions due to the existence of random exclusion zones;3)how to characterize the different types of interference and their impacts on the outage probabilities of cellular and D2D *** then run extensive Monte-Carlo simulations which manifest that our theoretical model is very accurate.

关键词： Device-to-device(D2D)-enabled heterogeneous cellular networks(HetCNets) exclusion zone stochastic geometry(SG) Matérn hard-core process(MHCP)

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Natural products inspired[3+2]cycloaddition enables efficient construction of hydroxylated tetrahydrofuran acetals and concise syntheses of lignans

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Science China Chemistry 2024年第7期67卷 2306-2313页

作者： Dong-yue Cai Weiming shi Zhao-Hui Jin Zhi-Xiang yu Jin-Xin Zhao Jian-Min yue State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China Beijing National Laboratory for Molecular Sciences(BNLMS) Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of ChemistryPeking UniversityBeijing 100871China

Mimicking biosynthetic pathways of hongkonoids led to the development of a new Cu(Ⅰ)-catalyzed[3+2]cycloaddition ofα-hydroxyketone andβ-keto enol ethers,affording tetrahydrofuran acetals in a highly diastereoselective manner and 100%atom *** studies on the mechanism disclosed a concerted but asynchronous Michael addition/aldol *** the same importance,this methodology provides a practical biomimetic approach for one-step construction of the dibenzylbutyrolactol lignan backbone starting from two phenyl propane derivatives,opening up a powerful new approach for lignan synthesis,which is showcased by succinct total syntheses of two biologically important aryltetralin-type lignans,β-apopicropodophyllin and *** the mild and operationally simple conditions,the developed chemistry might have a promising prospect in potential industrial applications.

关键词： [3+2]cycloaddition transition-metal catalysis tetrahydrofuran lignans natural products

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Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

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Opto-Electronic Advances 2024年第6期7卷 41-56页

作者： Xin Xu Qin Luo Jixiang Wang Yahui Song hong Ye Xin Zhang Yi He Minxuan Sun Ruobing Zhang Guohua shi School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230026China Jiangsu Key Laboratory of Medical Optics Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of ScienceSuzhou 215163China

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view,typically around 1 *** restriction poses a challenge when attempting to simultaneously analyze various parts of a sample,such as different brain *** addition,conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in *** we present a novel mesoscopic objective lens with an impressive field of view of 8 mm,a numerical aperture of 0.5,and a working wavelength range from 400 to 1000 *** achieved a resolution of 0.74μm in fluorescent beads *** versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system,a confocal laser scanning system,and a two-photon imaging *** mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.

关键词： mesoscopic objective lens large field-of-view high resolution multi-wavelength wide-field microscopy confocal laser scanning microscopy

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