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Protective Effect of Silibinin on Lipopolysaccharide-Induced Endotoxemia by Inhibiting Caspase-11-Dependent Cell Pyroptosis

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Chinese Journal of Integrative Medicine 2024年第10期30卷 917-926页

作者： OU Jin-ying LIU Shan-hong TANG Dong-kai shi Ling-zhu YAN Li-jun HUANG Jing-yan ZOU Li-fang QUAN Jing-yu YOU Yan-ting CHEN yu-yao yu Lin-zhong LU Zi-bin Third Level Research Laboratory of State Administration of Traditional Chinese Medicine School of Traditional Chinese MedicineSouthern Medical UniversityGuangzhou510515China Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics Guangzhou510515China School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou510515China

Objective:To explore the protective effect and the underlying mechanism of silibinin(SIB),one of the active compounds from Silybum marianum(L.)Gaertn in ***:Mouse peritoneal macrophage were isolated via intraperitoneally injection of BALB/c mice with thioglycolate *** viability was assessed using the cell counting kit-8,while cytotoxicity was determined through lactate dehydrogenase cytotoxicity *** protein expressions of interleukin(IL)-1α,IL-1β,and IL-18 were determined by enzyme-linked immunosorbent *** lipopolysaccharide(LPS)levels were measured by employing both the limulus amoebocyte lysate assay and flow ***,proximity ligation assay was employed for the LPS and caspase-11 *** were divided into 4 groups:the control,LPS,high-dose-SIB(100 mg/kg),and low-dose-SIB(100 mg/kg)groups(n=8).Zebrafish were divided into 4 groups:the control,LPS,high-dose-SIB(200μmol/L),and low-dose-SIB(100μmol/L)groups(n=30 for survival experiment and n=10 for gene expression analysis).The expression of caspase-11,gasdermin D(GSDMD),and N-GSDMD was determined by Western blot and the expressions of caspy2,gsdmeb,and IL-1βwere detected using quantitative real-time *** observation was performed through hematoxylineosin staining,and protein levels in bronchoalveolar lavage fluid were quantified using the bicinchoninicacid protein ***:SIB noticeably decreased caspase-11 and GSDMD-mediated pyroptosis and suppressed the secretion of IL-1α,IL-1β,and IL-18 induced by LPS(P<0.05).Moreover,SIB inhibited the translocation of LPS into the cytoplasm and the binding of caspase-11 and intracellular LPS(P<0.05).SIB also attenuated the expression of caspase-11 and N-terminal fragments of GSDMD,inhibited the relative cytokines,prolonged the survival time,and up-regulated the survival rate in the endotoxemia models(P<0.05).Conclusions:SIB can inhibit pyroptosis in the LPS-mediated endotoxemia model,at l

关键词： silibinin caspase-11 endotoxemia pyroptosis inflammation

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Immunomodulatory Function of Pien Tze Huang in T Cell-Mediated Anti-tumor Activity against B16-F10,MC38 and Hep1-6 Tumor Models

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Chinese Journal of Integrative Medicine 2024年第4期30卷 348-358页

作者： FU yu-bing LIU Chen-feng WANG Jin-jia JI Xiao-lin TANG Rong-han LIAO Kun-yu CHEN Ling-yue hong Ya-zhen FAN Bin-bin WANG shi-cong LIU Wen-Hsien State Key Laboratory of Cellular Stress Biology School of Life SciencesFaculty of Medicine and Life ScienceXiamen UniversityXiamenFujian Province(361102)China Fujian Pien Tze Huang Enterprise Key Laboratory of Natural Medicine Research and Development ZhangzhouFujian Province(363000)China

Objective To investigate the anti-tumor effects of Pien Tze Huang(PZH)in mouse models of B16–F10 melanoma,MC38 colorectal cancer,Hep1-6 hepatocellular carcinoma and chemically induced hepatocellular carcinoma *** Various tumor models,including B16–F10,MC38 and Hep1-6 tumor hypodermic inoculation models,B16–F10 and Hep1-6 pulmonary metastasis models,Hep1-6 orthotopic implantation model,and chemically induced hepatocellular carcinoma model,were utilized to evaluate the anti-tumor function of *** growth was assessed by measuring tumor size and weight of solid tumors isolated from C57BL/6 *** cell proliferation and death of tumor cells in vitro,as well as T cell activation markers,cytokine production and immune checkpoints analysis,single-cell suspensions were prepared from mouse spleen,lymph nodes,and tumors after PZH *** PZH demonstrated significant therapeutic efficacy in inhibiting tumor growth(P0.05).Nevertheless,PZH exhibited a remarkable ability to enhance T cell activation and the production of interferon gamma,tumor necrosis factor alpha,and interleukin 2 in CD4^(+)T cells in vitro(P<0.01 or P<0.05).Importantly,PZH substantially inhibited T cell exhaustion and boosted cytokine production by tumor-infiltrating CD8^(+)T cells(P<0.01 or P<0.05).Conclusion This study has confirmed a novel immunomodulatory function of PZH in T cell-mediated anti-tumor immunity,indicating that PZH holds promise as a potential therapeutic agent for cancer treatment.

关键词： Pien Tze Huang metastasis anti-tumor immunity T cell activation T cell exhaustion immune checkpoints Chinese medicine

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Robust tantalum tuned perovskite oxygen electrode for reversible protonic ceramic electrochemical cells

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Rare Metals 2024年第7期43卷 3055-3065页

作者： yu-shi Feng yu Shen Fang Wang Chuan Tian Qin-Yi Hu Qian Duan Jing-Wei Li Di Bao School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun 130022China Zhongshan Institute of Changchun University of Science and Technology Zhongshan528437China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun 130022China Key Laboratory of Preparation and Applications of Environmental Friendly Materials Ministry of EducationJilin Normal UniversityChangchun 130103China

Perovskite oxides with diverse composition and structure have exhibited grand advances in boosting the oxygen reduction and evolution reaction(ORR/OER),which are essential for the reversible protonic ceramic electrochemical cell(R-PCEC)toward the sustainable hydrogen production and ***,enhancement of their activity and stability remains ***,we develop the Ta-regulated BaCo_(0.7)Fe_(0.3)O_(3-δ)perovskite oxygen electrode(Ba(Co_(0.7)Fe_(0.3))_(1-x)Ta_xO_(3-δ))with abundant oxygen defects and achieve the simultaneous enhancement in the electrocatalytic activity and stability toward ORR and ***-fabricated R-PCEC with(Ba(Co_(0.7)Fe_(0.3))_(0.9)Ta_(0.1)O_(3-δ))(BCFT10)oxygen electrode performs high power density of 1.47 W·cm^(-2)at 650℃in fuel cell mode,and the current density is up to-2.11 A·cm^(-2)at 1.4 V at 650℃in electrolysis mode,as well as the good stability in both the fuel cell and electrolysis ***,the cell also demonstrates a stable cycling operation between fuel cell and electrolysis mode,suggesting a great potential of BCFT10 as oxygen electrode material for R-PCECs.

关键词： Reversible protonic ceramic electrochemical cells Perovskite oxide Oxygen electrode Ta element doping Oxygen defects

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Displacement-pressure biparametrically regulated softness sensory system for intraocular pressure monitoring

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National Science Review 2024年第6期11卷 159-168页

作者： yu Cheng Yifei Zhan Fangyi Guan Junli shi Jingxiao Wang Yi Sun Muhammad Zubair Cunjiang yu Chuan Fei Guo Department of Materials Science and Engineering Southern University of Science and Technology Department of Engineering Science and Mechanics Pennsylvania State University Department of Biomedical Engineering Pennsylvania State University Department of Materials Science and Engineering Materials Research Institute Pennsylvania State University

High intraocular pressure(IOP) is one of the high-risk pathogenic factors of glaucoma. Existing methods of IOP measurement are based on the direct interaction with the cornea. Commercial ophthalmic tonometers based on snapshot measurements are expensive, bulky, and their operation requires trained *** contact lenses are easy to use, but they may block vision and cause infection. Here, we report a sensory system for IOP assessment that uses a soft indentor with two asymmetrically deployed iontronic flexible pressure sensors to interact with the eyelid-eyeball in an eye-closed situation. Inspired by human fingertip assessment of softness, the sensory system extracts displacement-pressure information for soft evaluation, achieving high accuracy IOP monitoring(>96%). We further design and custom-make a portable and wearable ophthalmic tonometer based on the sensory system and demonstrate its high efficacy in IOP screening. This sensory system paves a way towards cost-effective, robust, and reliable IOP monitoring.

关键词： intraocular pressure tonometer deep learning softness iontronic sensor

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Engineering self-catabolic DNAzyme nanospheres for synergistic anticancer therapy

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Science China Chemistry 2024年第7期67卷 2412-2422页

作者： yu Chen yu Guo Jiaoli Wang Ruiting Liu Xiaohai Yang Kemin Wang Ying Pu Hui shi Jin Huang Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringKey Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan ProvinceHunan UniversityChangsha 410082China National Clinical Research Center for Geriatric Disorders Department of GeriatricsXiangya HospitalCentral South UniversityChangsha 410008China

DNAzyme-based gene therapy faces some challenges including cell penetration,activity limitation,and co-delivery ***-assembled DNA nanomedicine has attracted widespread attention due to its many *** is urgent to develop a universal DNA degradation strategy for precise programmable drug ***,we reported a self-catabolic DNAzyme nanospheres(SCNS),which could simultaneously achieve cell penetration,activity enhancement,and co-delivery *** SCNS were assembled through Y-DNA stepwise hybridization with each other,which were then loaded with aptamer(Apt),doxorubicin(Dox),and zinc oxide nanoparticles(ZnO NPs).The acid-triggered dissociation of ZnO NPs leads to the generation of Zn^(2+)ions cofactors for immediately self-catabolic DNAzyme *** the disassembly of the SCNS,three types of anticancer treatments would be activated,which include Zn^(2+)involved reactive oxygen species(ROS),Dox-induced chemotherapy,and DNAzyme-based gene *** experimental results show that the nanoplatform(Apt-SCNS-Dox-ZnO)has a good tumor-killing effect and minimal side *** a smart self-driven drug delivery nanoplatform,it is anticipated to displace extraordinary potential in biomedicine and bioengineering.

关键词： DNAzyme nanosphere ZnO NPs ROS Dox anticancer therapy

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Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance

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Plant Communications 2024年第6期5卷 139-153页

作者： Zheng-Wei Fu Feng Ding Bing-Lei Zhang Wen-Cheng Liu Zi-hong Huang shi-Hang Fan yu-Rui Feng Ying-Tang Lu Wei Hua Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsOil Crops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China State Key Laboratory of Hybrid Rice College of Life SciencesWuhan UniversityWuhan 430072China School of Landscape and Ecological Engineering Hebei University of EngineeringHandan 056038China State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityKaifeng 475004China Hubei hongshan Laboratory Wuhan 430070China

Climate change is resulting in more frequent and rapidly changing temperatures at both extremes that severely affect the growth and production of plants,particularly *** stress caused by high temperatures is one of the most damaging factors for ***,the role of hydrogen peroxide(H_(2)O_(2))in modulating plant thermotolerance is largely unknown,and the regulation of photorespiration essential for C3 species remains to be fully ***,we report that heat stress promotes H2O2 accumulation in chloroplasts and that H2O2 stimulates sulfenylation of the chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1(PGLP1)at cysteine 86,inhibiting its activity and promoting the accumulation of the toxic metabolite *** also demonstrate that PGLP1 has a positive function in plant thermotolerance,as PGLP1 antisense lines have greater heat sensitivity and PGLP1-overexpressing plants have higher heat-stress tolerance than the wild ***,our results demonstrate that heat-induced H2O2 in chloroplasts sulfenylates and inhibits PGLP1 to modulate plant ***,targeting CATALASE2 to chloroplasts can largely prevent the heatinduced overaccumulation of H2O2 and the sulfenylation of PGLP1,thus conferring thermotolerance without a plant growth *** findings reveal that heat-induced H2O2 in chloroplasts is important for heat-caused plant damage.

关键词： heat stress photorespiration hydrogen peroxide PGLP1 2-phosphoglycolate

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端壁合成射流对高负荷涡轮叶栅涡系结构和流动损失影响的数值研究

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推进技术 2018年第1期39卷 33-45页

作者：于洪石王志恒席光西安交通大学能源与动力工程学院陕西西安710049

为探究端壁合成射流对高负荷涡轮叶栅中涡系结构和流动损失的影响,采用非定常数值模拟方法分析了不同激励参数下合成射流对Durham叶栅流动损失的控制效果以及涡系结构和流动损失的对应关系。结果表明,合成射流减小了前缘马蹄涡和通道涡... 详细信息

为探究端壁合成射流对高负荷涡轮叶栅中涡系结构和流动损失的影响,采用非定常数值模拟方法分析了不同激励参数下合成射流对Durham叶栅流动损失的控制效果以及涡系结构和流动损失的对应关系。结果表明,合成射流减小了前缘马蹄涡和通道涡的尺度,削弱了来自相邻叶片压力面的横向涡,略微增强了壁角涡,并间接削弱了壁面涡;在无量纲幅值和频率分别为0.073和1时,控制效果最佳,总压损失系数减小约为10.72%;从控制机理上讲,合成射流加强了主流和射流下游边界层的掺混,增加了边界层动量,从而削弱了马蹄涡;合成射流影响了叶片压力面的流动分离,改变了由于分离产生低能流体的位置和范围,从而削弱了横向涡。由于漩涡的削弱,流动损失也随之减小。

关键词：合成射流激励参数主动流动控制漩涡结构流动损失

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Recent advances in droplet-based microfluidics in liquid biopsy for cancer diagnosis

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Droplet 2024年第1期3卷 1-27页

作者： Jingyu shi yu Zhang Yadi Fan Yi LiuMo Yang Department of Biomedical Engineering The Hong Kong Polytechnic UniversityHong KongChina Department of Mechanical and Automotive Engineering Royal Melbourne Institute of TechnologyMelbourneAustralia

Liquid biopsy,a noninvasive technique to obtain tumor information from body fluids,is an emerging technology for cancer diagnosis,prognosis,and monitoring,providing crucial support for the realization of precision *** main biomarkers of liquid biopsy include circulating tumor cells,circulating tumor DNA,microRNA,and circulating tumor *** liquid biopsy detection methods include flow cytometry,immunoassay,polymerase chain reaction(PCR)-based methods,and next-generation sequencing(NGS)-based methods,which are time-consuming,labor-intensive,and cannot reflect cell ***-based microfluidics with high throughput,low contamination,high sensitivity,and single-cell/single-molecule/single-exosome analysis capabilities have shown great potential in the field of liquid *** review aims to summarize the recent development in droplet-based microfluidics in liquid biopsy for cancer diagnosis.

关键词： diagnosis biopsy fluid

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Potassium isotopic signatures of modern offshore detrital sediments from different climatic regimes and the implications

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Science China Earth Sciences 2024年第2期67卷 405-419页

作者： Jun MU Tianyu CHEN Qian yu shichao AN Jianfang CHEN Xuefa shi Weiqiang LI State Key Laboratory for Mineral Deposits Research School of Earth Sciences and EngineeringNanjing UniversityNanjing210023China Ministry of Education Key Laboratory for Coast and Island Development School of Geography and Ocean ScienceNanjing UniversityNanjing210023China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou310012China Key Laboratory of Marine Geology and Metallogeny First Institute of OceanographyMinistry of Natural ResourcesQingdao266061China

Potassium isotopes are a novel tracer for continental *** K isotope studies on chemical weathering generally targeted weathering profiles under a particular climate region,yet the effects of chemical weathering on K isotopes under different climatic backgrounds remain ***,little is known about the K isotope signatures of modern unconsolidated detrital ***,we report K isotopic data of surficial seafloor sediments from continental shelves along the east coast of China(ECC),as well as those around the tropical Hainan island in the northern South China *** ECC sediments have a relatively narrow distribution ofδ^(41)K(with reference to NIST3141a)values,which range from(-0.40±0.01)‰to(-0.57±0.04)‰,with an average of(-0.51±0.09)‰.By contrast,δ^(41)K values of Hainan offshore sediments display a larger variation,ranging from(-0.28±0.07)‰to(-0.67±0.02)‰.Theδ^(41)K values of Hainan offshore sediments exhibit negative correlations with the chemical index of alteration(CIA),Al/K,Ti/K,and total iron(FeT),which underlines the control of chemical weathering on K isotopic signatures of detritus inputs into *** also measured Mg isotope compositions for the same samples;interestingly,the variability inδ^(26)Mg of the samples is small(~0.24‰)for all ECC and Hainan offshore sediments,andδ^(26)Mg values do not show clear correlations with indexes of chemical *** study demonstrates the link between K isotopic variability of detrital sediments and climatic conditions including rainfall intensity,which indicates that K isotopes of the detrital component of marine sediments could be applied to study Earth’s climate in deep ***δ^(41)K values of the offshore detrital sediments are significantly less variable than those of pelagic marine sediments,highlighting the importance of distinguishing the effects of diagenesis and neoformation of clay minerals from continental weathering in attempts to study deep-time climate-weathering

关键词： Potassium isotopes Magnesium isotopes Chemical weathering Detrital sediments Climatic regimes Mean annual precipitation

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Preparation and properties of ZrO_(2)-strengthened porous mullite insulation materials using Y_(2)O_(3) additive

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Journal of Iron and Steel Research International 2024年第6期31卷 1514-1521页

作者： Wang-zhi yu Yi-hong shi Ping Liu Yong-wan Wu Cheng-hao Song Xian-gong Deng Xiang Ding Song-lin Ran Yi Zhang Hai-jun Zhang Hai-liang Deng Advanced Ceramics Research Center School of Materials Science and EngineeringAnhui University of TechnologyMa'anshan 243002AnhuiChina The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina

ZrO_(2)-strengthened porous mullite insulation materials were prepared by foaming technology utilizing ZrSiO_(4) and Al_(2)O_(3) as primary materials and Y_(2)O_(3) as an *** effects of Y_(2)O_(3) contents on the phase composition,microstructure,mechanical properties,and heat conductivity of the porous mullite insulation materials were investigated.A suitable Y_(2)O_(3) content could promote phase transition of monoclinic ZrO_(2)(m-ZrO_(2))to tetragonal ZrO_(2)(t-ZrO_(2)),reduce pore size,and improve the strengths of as-prepared *** cold crushing strength and bending strength of as-prepared specimens with a 119µm spherical pore size using 6 wt.%Y_(2)O_(3) were 35.2 and 13.0 MPa,respectively,with a heat conductivity being only 0.248 W/(m K).

关键词： Porous mullite insulation material Zirconium oxide Y_(2)O_(3) Mechanical property Heat conductivity

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