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Neural Regeneration Research 2025年第7期20卷 1883-1899页

作者： Mingyu Zhang Chunyu Xiang Renrui Niu Xiaodong He Wenqi luo Wanguo Liu Rui Gu Department of Orthopedic Surgery China-Japan Union Hospital of Jilin UniversityChangchunJilin ProvinceChina

Various nanoparticle-based drug delivery systems for the treatment of neurological disorders have been widely ***,their inability to cross the blood–brain barrier hampers the clinical translation of these therapeutic *** are nanoparticles composed of lipid bilayers,which can effectively encapsulate drugs and improve drug delivery across the blood–brain barrier and into brain tissue through their targeting and ***,they can potentially treat traumatic and nontraumatic central nervous system *** this review,we outlined the common properties and preparation methods of liposomes,including thin-film hydration,reverse-phase evaporation,solvent injection techniques,detergent removal methods,and microfluidics ***,we comprehensively discussed the current applications of liposomes in central nervous system diseases,such as Alzheimer's disease,Parkinson's disease,Huntington's disease,amyotrophic lateral sclerosis,traumatic brain injury,spinal cord injury,and brain *** studies related to liposomes are still in the laboratory stage and have not yet entered clinical ***,their application as drug delivery systems in clinical practice faces challenges such as drug stability,targeting efficiency,and ***,we proposed development strategies related to liposomes to further promote their development in neurological disease research.

关键词： Alzheimer's disease amyotrophic lateral sclerosis brain tumors central nervous system Huntington's disease liposome drug delivery neurological disorders Parkinson's disease spinal cord injury traumatic brain injury

Perfluoropentane-based oxygen-loaded nanodroplets reduce microglial activation through metabolic reprogramming

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Neural Regeneration Research 2025年第4期20卷 1178-1191页

作者： Wanxian luo Chuanhui Xu Linxi Li Yunxiang Ji Yezhong Wang Yingjia Li Yongyi Ye Department of Medicine Ultrasonics Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Institute of Neuroscience Department of NeurosurgeryThe Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong ProvinceChina

Microglia,the primary immune cells within the brain,have gained recognition as a promising therapeutic target for managing neurodegenerative diseases within the central nervous system,including Parkinson’s *** perfluorocarbon droplets have been reported to not only possess a high oxygen-carrying capacity,but also exhibit remarkable anti-inflammatory ***,the role of perfluoropentane in microglia-mediated central inflammatory reactions remains poorly *** this study,we developed perfluoropentane-based oxygen-loaded nanodroplets(PFP-OLNDs)and found that pretreatment with these droplets suppressed the lipopolysaccharide-induced activation of M1-type microglia in vitro and in vivo,and suppressed microglial activation in a mouse model of Parkinson’s *** suppression led to a reduction in the inflammatory response,oxidative stress,and cell migration capacity in ***,the neurotoxic effects were mitigated,which alleviated neuronal ***,ultrahigh-performance liquid chromatography–tandem mass spectrometry showed that the anti-inflammatory effects of PFP-OLNDs mainly resulted from the modulation of microglial metabolic *** further showed that PFP-OLNDs regulated microglial metabolic reprogramming through the AKT-mTOR-HIF-1α***,our findings suggest that the novel PFP-OLNDs constructed in this study alleviate microglia-mediated central inflammatory reactions through metabolic reprogramming.

关键词： metabolic reprogramming microglia microglial migration nanotherapy neurodegenerative diseases neuroinflammation oxygen-loaded nanodroplets Parkinson’s disease perfluoropentane ultra-performance liquid chromatography–mass spectrometry

Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

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Neural Regeneration Research 2025年第5期20卷 1364-1376页

作者： Ronglin Han Lanxin luo Caiyan Wei Yaru Qiao Jiming Xie Xianchao Pan Juan Xing Department of Pathophysiology School of Basic Medical SciencesSouthwest Medical UniversityLuzhouSichuan ProvinceChina Department of Medicinal Chemistry School of PharmacySouthwest Medical UniversityLuzhouSichuan ProvinceChina

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

关键词： alginate axon growth biomaterials extracellular matrix neural repair neurons neuroregeneration polyacrylamide polydimethylsiloxane stiffness

平肝潜阳药物对偏头痛肝阳上亢证患者的临床疗效及其对血淋巴细胞蛋白质表达的影响

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中西医结合学报 2009年第1期7卷 25-33页

作者：钟广伟李炜罗艳红陈国林易振佳胡建军张莺尹耀慧张乐中南大学湘雅医院中西医结合研究所湖南长沙410008 中南大学湘雅医院急诊科湖南长沙410008 中南大学湘雅医院神经内科湖南长沙410008

目的:观察平肝潜阳药物对偏头痛肝阳上亢证患者的临床疗效和对血淋巴细胞蛋白质表达的影响,为深入研究偏头痛肝阳上亢证病理生理机制及平肝潜阳法治疗作用的分子机制奠定基础。方法:32例偏头痛肝阳上亢证患者随机分为治疗组和对照组。... 详细信息

目的:观察平肝潜阳药物对偏头痛肝阳上亢证患者的临床疗效和对血淋巴细胞蛋白质表达的影响,为深入研究偏头痛肝阳上亢证病理生理机制及平肝潜阳法治疗作用的分子机制奠定基础。方法:32例偏头痛肝阳上亢证患者随机分为治疗组和对照组。治疗组口服平肝潜阳方,对照组口服氟桂利嗪片剂。治疗2个疗程。观察患者治疗前后的中医症状积分、止痛疗效和不良反应;采用双向凝胶电泳、基质辅助激光解吸电离-飞行时间质谱法分离和鉴定偏头痛患者外周血淋巴细胞和正常人之间差异蛋白表达,并观察应用平肝潜阳方干预后,各组差异蛋白质表达水平的变化。结果:平肝潜阳方有明显止痛效果(87.5%),与氟桂利嗪片(75.0%)比较,差异有统计学意义(P<0.01);以改善眩晕、烦躁易怒和面部烘热症状更为明显(P<0.05)。两组中医症状积分均降低(P<0.01),其中以治疗组更为明显(P<0.01),治疗组未出现明显不良反应。正常人、平肝潜阳方治疗组偏头痛肝阳上亢证患者治疗前和治疗后血淋巴细胞凝胶蛋白质点数依次为534±42、552±54和529±55。平肝潜阳方治疗组偏头痛肝阳上亢证患者与正常人比较有6个蛋白质表达下调,14个蛋白质表达上调;平肝潜阳方治疗组偏头痛肝阳上亢证患者治疗前后比较发现表达下调的6个蛋白质中有4个表达增强,而表达上调的14个蛋白质中有11个表达降低。共得到12个肽质量指纹图谱,2个无质谱结果,搜索到10个具有统计学意义的蛋白质,主要有通道蛋白、抗氧化蛋白、细胞信号转导蛋白、代谢相关蛋白和免疫相关蛋白等。结论:平肝潜阳中药复方有良好的止痛作用,能改善中医症状;可能通过调节部分血淋巴细胞蛋白质的表达治疗偏头痛肝阳上亢证。

关键词：平肝潜阳肝阳上亢蛋白质组学偏头痛随机对照试验

Optimized finite difference iterative scheme based on POD technique for 2D viscoelastic wave equation

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Applied Mathematics and Mechanics(English Edition) 2017年第12期38卷 1721-1732页

作者： hong XIA Zhendong luo School of Control and Computer Engineering North China Electric Power UniversityBeijing 102206China School of Mathematics and Physics North China Electric Power UniversityBeijing 102206China

This study develops an optimized finite difference iterative （OFDI） scheme for the two-dimensional （2D） viscoelastic wave equation. The OFDI scheme is obtained using a proper orthogonal decomposition （POD） method. It has sufficiently high accuracy with very few unknowns for the 2D viscoelastic wave equation. Existence, stability, and convergence of the OFDI solutions are analyzed. Numerical simulations verify efficiency and feasibility of the proposed scheme.

关键词： optimized finite difference iterative (OFDI) scheme viscoelastic wave equation proper orthogonal decomposition (POD) existence stability convergence numericalsimulation

超声波、电场和光催化协同降解有机污染物的研究现状

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四川环境 2005年第1期24卷 65-67,118页

作者：汤红妍罗亚田徐昌伟武汉理工大学资源与环境工程学院武汉430070

以自由基反应为主的高级氧化工艺是处理有机污染物的新型技术,具有良好的拓展和应用环境。超声氧化、电化学氧化工艺和紫外光催化工艺都属于高级氧化工艺,许多研究者发现单独使用这些工艺降解有机污染物效果不够理想,更有效的方法是联... 详细信息

以自由基反应为主的高级氧化工艺是处理有机污染物的新型技术,具有良好的拓展和应用环境。超声氧化、电化学氧化工艺和紫外光催化工艺都属于高级氧化工艺,许多研究者发现单独使用这些工艺降解有机污染物效果不够理想,更有效的方法是联合使用。本文系统地介绍了超声波和光催化、光催化和电场、超声波和电场协同降解有机污染物的机理和研究现状。

关键词：高级氧化工艺联用技术有机污染物

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福氏志贺群7因子血清免疫方法的探讨

微生物学免疫学进展 2011年第2期39卷 12-14页

作者：金红燕罗广席亮杨福军陈国怀兰州生物制品研究所兰州730046

为了提高福氏志贺菌免疫的群7因子的血清效价和优化免疫方法,以两种免疫原成分,按两种浓度、剂量免疫动物后定期采集血清。用凝集试验检测血清抗体效价,比较新法和传统方法免疫家兔体内的抗体水平。结果显示,传统方法免疫的家兔所产生... 详细信息

为了提高福氏志贺菌免疫的群7因子的血清效价和优化免疫方法,以两种免疫原成分,按两种浓度、剂量免疫动物后定期采集血清。用凝集试验检测血清抗体效价,比较新法和传统方法免疫家兔体内的抗体水平。结果显示,传统方法免疫的家兔所产生的抗体1∶1280,而新法免疫中高剂量组可使福氏志贺群7因子血清效价达到1∶2560的水平,完全可以用于常规生产。

关键词：福氏志贺群7因子免疫抗原血清效价

Neurons derived from human-induced pluripotent stem cells express mu and kappa opioid receptors

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Neural Regeneration Research 2021年第4期16卷 653-658页

作者： Zhi-Hai Ju Xuan Liang Yao-Yao Ren luo-Wa Shu yan-hong yan Xu Cui Department of Anesthesiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina

Neuroprotection studies have shown that induced pluripotent stem(iPS)cells have the possibility to transform neuroprotection *** the present study,iPS cells were generated from human renal epithelial cells and were then differentiated into *** in the iPScell group were maintained in stem cell *** contrast,cells in the iPS-neuron group were first maintained in neural induction medium and expansion medium containing ROCK inhibitors,and then cultivated in neuronal differentiation medium and neuronal maturation medium to induce the neural stem cells to differentiate into *** expression of relevant markers was compared at different stages of *** staining revealed that cells in the iPS-neuron group expressed the neural stem cell markers SOX1 and nestin on day 11 of induction,and neuronal markers TUBB3 and NeuN on day 21 of *** chain reaction results demonstrated that,compared with the iPS-cell group,TUBB3 gene expression in the iPS-neuron group was increased *** research revealed that,compared with the iPS-cell group,the gene expression and immunoreactivity of mu opioid receptor in the iPS-neuron group were significantly increased(38.3-fold and 5.7-fold,respectively),but those of kappa opioid receptor had only a slight change(1.33-fold and 1.57-fold increases,respectively).Together,these data indicate that human iPS cells can be induced into mu opioid receptor-and kappa opioid receptor-expressing neurons,and that they may be useful to simulate human opioid receptor function in vitro and explore the underlying mechanisms of human conditions.

关键词： brain cells factor in vivo neural differentiation protein stem cells stroke

Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods

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Petroleum Science 2023年第6期20卷 3394-3399页

作者： Yunyang Wan hongMei Mu Na luo JianPing yang yan Tian Ning hong HaiLiang Dong National Key Laboratory of Petroleum Resources and Engineering Research Centre for Geomicrobial Resources and ApplicationBeijing Key Laboratory of Petroleum Pollution and ControlUnconventional Petroleum Research InstituteChina University of PetroleumBeijing102249China Drilling and Production Engineering Technology Department of PetroChina Liaohe Oilfield Company Panjin124010LiaoningChina Center for Geomicrobiology and Biogeochemistry Research State Key Laboratory of Biology and Environmental GeologyChina University of GeosciencesBeijing100083China

DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.

关键词： Heavy oil DNA concentration DNA purity Spectrophotometry qPCR

The attenuation of oscillatory thermo-capillary convection in the oxide melt by a transverse magnetic field

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Science China(Physics,Mechanics & Astronomy) 2007年第4期50卷 519-524页

作者： JIN WeiQing AI Fei hong Yong luo HaoSu LIU yan PAN Xiuhong Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050China

The effect of a transverse magnetic field on the oscillatory thermocapillary con- vection in the NaBi(WO4)2 melt was studied by using the in-situ observation system. The oscillation was attenuated when the 60 mT magnetic field was applied, as shown by the decrease in the amplitude and the frequency. Furthermore, the os- cillation under smaller temperature difference was stabilized after the magnetic field was applied. The magnetic effect could be due to the Lorentz force generated by the interaction between motional ions and the vertical magnetic field. The ionic conductivities were measured to demonstrate the effect of the magnetic field. The solid ionic electrical conductivity increases with the temperature rise, and the melt ionic electrical conductivity was measured to be about 2.0×10-4 -1cm-1. Experi- mental results manifest that the effect of the magnetic field on anions and cations in the melt makes the flow change to the direction normal to the applied field, so the flow is more orderly and the oscillation is suppressed.

关键词： oscillatory thermocapillary convection high-temperature melt in-situ observation magnetic field