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The Role of Propeptide in the Refolding of Human Group IB Phospholipase A2

Acta Biochimica et Biophysica Sinica 2004年第9期36卷 583-588页

作者： hong-qiang cheng1 and gen-jun xu1,2.institute of biochemistry and cell biology,{2.,{3., shanghai 2.003., China 2.ollege of Life Science, Zhejiang University of sciences, Hangzhou 3.003., China institute of biochemistry and {2.{3. Shanghai Institutes for Biological Sciences Chinese Academy of Sciences College of Life Science Zhejiang University of Sciences

Human group IB phospholipase A2.(IB-PLA2. and its zymogen (proIB-PLA2. were purified from E. coli. Refolding was carried out by diluting the denatured forms of both IB-PLA2.and proIB-PLA2. with renaturation buffer in which the disulfide bonds were completely reduced. The refolding yield of proIB-PLA2.was increased by about 50% over that of the mature enzyme. The refolding of IB-PLA2.usually produced aggregates under normal conditions, as determined by light scattering. In addition, the unfolding experiments showed that the mature enzyme was more stable than the proenzyme toward denaturants in the presence of DTT. Results suggested that the N-terminal sequence rather than its conformation of human proIB-PLA2.played an important role in the refolding process.

关键词： human group IB phospholipase A2.(IB-PLA2. refolding first disulfide bond propeptide

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Opposite Sex Contact and Isolation: A Novel Depression/Anxiety Model

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Neuroscience Bulletin 2016年第1期32卷 92-98页

作者： Liu Yang Li-jun Shi Bei Tang Qiu-Qin Han Jin Yu gen-{2. Wu Yu-Qiu Zhang institute of biochemistry and {2.{3. Shanghai Institutes for Biological Sciences Chinese Academy of Sciences institute of Neurobiology Institutes of Brain Science and State Key Laboratory of Medical NeurobiologyCollaborative Innovation Center for Brain Science Liver Cancer institute Zhongshan Hospital Shanghai Medical College Fudan University Department of Integrative Medicine and Neurobiology Shanghai Medical College Fudan University

To mimic human mood disorders, traditional chronic stresses and social defeat stress have been developed and widely applied. However, these active stresses do not mimic the emotional flaws induced by stresses, and their input levels vary greatly. Also, emotional stresses resulting from social unobtainability remain largely elusive due to the lack of useful animal models. In this study, we developed a mouse model named ‘‘opposite sex contact and isolation’’(OSCI) and found that OSCI induced significant social avoidance, anhedonia, and anxiety. These behavioral defects developed differently after 7 days of OSCI. The social avoidance behavior was self-curable while anxiety gradually worsened but was alleviated by re-pairing with the same female partner. Corresponding to the behavior changes, the plasma corticosterone and phosphorylated c AMP response element binding protein levels were decreased in the nucleus accumbens of the mice that experienced isolation. Together,this study has developed a novel strategy for depression/anxiety modeling and shows that OSCI may be a useful tool for studying the lovelorn/lovesick type of depression.

关键词： Social isolation Depression Anxiety Opposite sex contact Social stress Lovelorn

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Diagnosis and Treatment Guidelines for Mesenchymal Stem cell Therapy for Coronavirus Disease 2.19(Beijing,2.2.)

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Infectious Diseases & Immunity 2021年第2期1卷 68-73页

作者： Ruonan xu Lei Shi Wei-Fen Xie Zhe xu Fan-Ping Meng {2.-Liang Fu Xin Yuan Lei Huang Ming Shi Yonggang Li Yuanyuan Li Chen Yao Yu Zhang Z{3.min Liu Chenyan Gao Zhaohui Wu Shufang Meng Weidong Han Charlie Xiang Gui-qiang Wang Taisheng Li Xiaoying Wang Yunxia Sun Zunyou Wu Wen{3. Zhang Chunhua Zhao Yu Hu Tao cheng Yuquan Wei Qi Zhou Fu-Sheng Wang Department of Infectious Diseases Fifth Medical Center of Chinese PLA General HospitalNational Clinical Research Center for Infectious DiseasesBeijing 100039China Department of Gastroenterology Changzheng Hospital of the Second Military Medical UniversityShanghai 200001China Department of Medical Statistics Peking University First HospitalBeijing 100191China National Industrial Base for Stem cell Engineering Products Tianjin 300384China Department of Cardiac Surgery Shanghai East HospitalTongji UniversityShanghai 310000China Office of Clinical Evaluation of biological Products Center for Drug EvaluationChina Food and Drug AdministrationBeijing 100053China China Medicinal Biotech Association Beijing 100022China National cell Collection and Research Center Institute for Biological Product ControlNational Institutes for Food and Drug ControlTiantanXiliDongcheng DistrictBeijing 100050China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseasesthe First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003China Department of Infectious Diseases and the Center for Liver Diseases Peking University First HospitalBeijing 100034China Department of Infectious Diseases Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijing 100730China Radiology Department Peking University First HospitalBeijing 100034China JOINN Laboratories(Beijing) Beijing 100176China chinese Center for Disease Control and Prevention Beijing 102206China Department of Infectious Diseases Huashan HospitalShanghai Medical CollegeFudan UniversityShanghai 200040China CAS Key Laboratory of Microbial Physiological and Metabolic Engineering State Key Laboratory of Microbial ResourcesInstitute of MicrobiologyChinese Academy of SciencesBeijing 100730China Union Hospital Tongji Medical CollegeHuazh

The coronavirus disease 2.19(COVID-19)can be caused by severe acute respiratory syndrome coronavirus 2.SARS-CoV-2.infection,and has led to millions of deaths among more than 100 million infected people around the world according to the declaration from World Health *** immune response of both the innate and adaptive immune systems is subsistent on COVID-19 patients,of which the degree are associated with disease severity,lung damage and long term functional *** treatment options have included antiretroviral drugs,anti-inflammatory factors,antibodies,immune checkpoint inhibitors,and convalescent plasma *** recently,mesenchymal stem {3.(MSC)therapy has been explored for the management and control of COVID-19,particularly with the aim of preventing or at least mitigating respiratory *** the safety and efficacy of stem {3. therapy have been validated in multiple phase I–III clinical trials,to date,no standardized stem {3. preparation,administration dosage or interval,product QA/QC testing,storage,transportation,or disposal protocols have been *** present paper proposes a systematic methodology that addresses all the foregoing process steps and evaluation criteria for the efficacious and safe administration of MSCs in the treatment of patients infected with COVID-19.

关键词： COVID-19 Mesenchymal stem cell Treatment

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