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Prox1 Suppresses Proliferation and Drug Resistance of Retinoblastoma Cells via Targeting Notch1

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Current Medical Science 2024年第1期44卷 223-231页

作者： hong-li zhang Na li lin DONG hong-xia MA Mo-chi YANG Department of Ophthalmology General Hospital of Ningxia Medical UniversityYinchuan 750004China

Objective Retinoblastoma(RB)is a prevalent type of eye cancer in *** homeobox 1(Prox1)is a homeobox transcriptional repressor and downstream target of the proneural gene that is relevant in lymphatic,hepatocyte,pancreatic,heart,lens,retinal,and cancer *** goal of this study was to investigate the role of Prox1 in RB cell proliferation and drug resistance,as well as to explore the underlying Notch1 *** Human RB cell lines(SO-RB50 and Y79)and a primary human retinal microvascular endothelial cell line(ACBRI-181)were used in this *** expression of Prox1 and Notch1 mRNA and protein in RB cells was detected using quantitative real time-polymerase chain reaction(RT-qPCR)and Western *** proliferation was assessed after Prox1 overexpression using the Cell Counting Kit-8 and the MTS ***-resistant cell lines(SO-RB50/vincristine)were generated and treated with Prox1 to investigate the role of Prox1 in drug *** employed pcDNA-Notch1 to overexpress Notch1 to confirm the role of Notch1 in the protective function of ***,a xenograft model was constructed to assess the effect of Prox1 on RB in *** Prox1 was significantly downregulated in RB *** of Prox1 effectively decreased RB cell growth while increasing the sensitivity of drug-resistant cells to ***1 was involved in Prox1’s regulatory ***1 was identified as a target gene of Prox1,which was found to be upregulated in RB cells and repressed by increased Prox1 *** pcDNA-Notch1 was transfected,the effect of Prox1 overexpression on RB was ***,by downregulating Notch1,Prox1 overexpression slowed tumor development and increased vincristine sensitivity in *** These data show that Prox1 decreased RB cell proliferation and drug resistance by targeting Notch1,implying that Prox1 could be a potential therapeutic target for RB.

关键词： Proxl Notch1 retinoblastoma cells proliferation drug resistance

Effects of an Engineered Anti-HER2 Antibody chA21 on Invasion of Human Ovarian Carcinoma Cell In Vitro

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Chinese Journal of Cancer Research 2011年第2期23卷 147-152页

作者： Yi Gao Qiang Wu Zheng-sheng Wu Gui-hong zhang An-li zhang Department of Pathology Anhui Medical University Hefei 230032 China

Objective： HER-2 plays an important role in the development and progression of ovarian carcinoma. A number of monoclonal antibodies （MAbs） and engineered antibody fragments （such as scFvs） against the subdomain II or IV of HER-2 extracellular domain （ECD） have been developed. We investigated the effect of chA21, an engineered anti-HER-2 antibody that bind primarily to subdomain I, on ovarian carcinoma cell invasion in vitro, and explored its possible mechanisms. Methods： Growth inhibition of SK-OV-3 cells was assessed using a Methyl thiazolyl tetrazolium （MTT） assay. The invasion ability of SK-OV-3 was determined by a Transwell invasion assay. The expression of matrix metalloproteinase-2 （MMP-2） and its tissue inhibitors （TIMP-2） was detected by immunocytochemical staining, and the expression of p38 and the phosphorylation of p38 were assayed by both immunocytochemistry and Western blot. Results： After treatment with chA21, the invasion of human ovarian cancer SK-OV-3 cells was inhibited in dose- and time-dependent manners. Simultaneously the expression of p38, phospho-p38, MMP-2 and the MMP-2/TIMP-2 ratio decreased, while TIMP-2 expression increased. Additionally, the decrease in phospho-p38 was much greater than that of p38. Conclusion： chA21 may inhibit SK-OV-3 cell invasion via the signal transduction pathway involving MMP-2, TIMP-2, p38 and the activation of p38MAPK.

关键词： HER-2 Ovarian neoplasms Invasion p38 mitogen-activated protein kinase Matrix metalloproteinase-2 Tissue inhibitor of matrix metalloproteinase-2

Kinetic Study of SiO_2/S Coating Deposition by APCVD

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Journal of Materials Science & Technology 2007年第4期23卷 535-540页

作者： Jianxin ZHOU hong XU li zhang Jinglei liU Xuegui QI Bo PENG State Key Laboratory of Chemical Engineering East China University of Science and Technology Shanghai 200237 China

To alleviate catalytic coking on the inner surface of radiant tube for ethylene production in petrochemical plants,SiO2/S coatings were deposited on HP40 alloy specimens using dimethyldisulfide （DMDS） and tetraethoxysilane （TEOS） by atmospheric pressure chemical vapor deposition （APCVD）. A two-dimension mathematical model was made to predict the growth rate of SiO2/S coating and to study the effects of deposition parameters on the deposition rate. The results show that the predicted deposition rate is in good agreement with the experimental one. The deposition rate mainly depends on the concentrations of precursors in the total gas flow, concentrations of intermediates on the deposition surface, total gas flow rate and deposition temperature. The weight of SiO2/S coating linearly increases with the deposition time. When the gas flow rate is below 0.3 m/s, the rate-limiting step of SiO2/S coating deposition is the diffusions of ***, the surface reactions of intermediates will be the rate-limiting step after the gas flow rate is above 0.3 m/s. When the deposition temperature is below 780℃, the rate-limiting step of SiO2/S coating deposition mainly depends on the surface reactions of intermediates. When the deposition temperature is above 780℃,the rate-limiting step depends on the diffusions of intermediates. The deposition rate increases with increasing the concentrations of the intermediates. However, when the partial pressures of the intermediates reach 8 Pa,the deposition rate keeps constant.

关键词： APCVD SiO2/S coating Mathematical model HP40 steel Kinetics

Blimp-1 protein and Hans classification on prognosis of diffuse large B-cell lymphoma and their interrelation

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Chinese Journal of Cancer 2010年第9期29卷 781-786页

作者： Yan Song Zhi Cao ling li hong-Tu zhang Xun zhang Department of Pathology Cancer Institute and Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing100021 P. R. China

Background and Objective:Diffuse large B-cell lymphoma (DLBCL), the most common type of non-Hodgkin's lymphoma (NHL), is heterogeneous on molecular and clinical levels, therefore, its prognosis is difficult to *** study aimed to evaluate the value of Blimp-1 protein and Hans classification in predicting the prognosis of DLBCL and their ***:The clinical records of 136 patients with DLBCL were *** patients were followed up for 5-80 months (median, 39 months).Immunohistochemical staining for CD10, MUM1, Bcl-6, and Blimp-1 were performed on paraffin-embedded tumor tissues from the 136 *** correlations of Blimp-1 protein and Hans classification in prognosis of DLBCL and their interrelation were ***:Blimp-1 was detected in 38 (30.0%) patients, and was associated with a significantly shorter overall survival (OS) (P=0.030).Using the Hans classification based upon the expression of CD10, Bcl-6, and MUM1, 54 patients had germinal center B-cell (GCB) phenotype and 82 had non-GCB *** 5-year OS rate was 75% in the GCB group and 52% in the non-GCB group (P=0.020).The positive rate of Blimp-1 was 22.2% in the GCB group and 31.7% in the non-GCB group (P=0.329).The Cox regression multivariate analysis showed that international prognosis index (IPI) and Hans classification had independent prognostic significance, whereas Blimp-1 was not an independent prognostic ***:The patients with GCB subtype of DLBCL had better prognosis than the non-GCB *** level of Blimp-1 expression in the patients with DLBCL implies a shorter survival, but it is not associated with Hans classification.

关键词： B细胞淋巴瘤分类价值弥漫性预后飞艇蛋白发电机断路器免疫组织化学染色

Double Slag Operation Dephosphorization in BOF for Producing Low Phosphorus Steel

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Journal of Iron and Steel Research International 2009年第3期16卷 6-14页

作者： TIAN Zhi-hong li Ben-hai zhang Xiao-ming JIANG Zhong-hang Shougang Research Institute of Technology Shougang Group Beijing 100041 China

A study on the production of low phosphorus steel by double slag operation in 210 t converter was carried out. A phosphorus content of less than 0.005% （mass percent） was obtained before tapping. About 80% phosphorus could be removed by the first slag after 5 min. High Fe^3＋ content and high basicity in the first slag were in favor of dephosphorization. On the other hand,Fe3＋ content had less effect on dephosphorization during second slag treatment. In the second slag period,the fraction of dephosphorization increased with the increase of basicity up to a basicity of 6. Further increase of basicity of the second slag had very little effect on dephosphorization. The tapping temperature had great impact on dephosphorization. It was impossible to get phosphorus less that 0.005% when the tapping temperature was higher than 1 943 K. The optimum operation conditions were suggested. On the basis of these conditions,the amount of the second slag and the effect of the remaining first slag were estimated.

关键词： dephosphorization double slag low phosphorus steel converter

Enantiomeric Separation of Four Chiral Compounds Using Immobilized Cellulose 3, 5-Dimethylphenylcarbamate as Chiral Stationary Phase

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Chinese Chemical Letters 2006年第9期17卷 1213-1216页

作者： Yong Fei MING liang ZHAO hong li zhang Yan Ping SHI Yong Min li li Ren CHEN Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 The Graduate School of Chinese Academy of Sciences Beijing 100039

A new chiral stationary phase of 3, 5-dimethylphenylcarbamates of cellulose, chemically bonded to 3-aminopropylsilica gel at the 6-positions of the glucose units, was prepared. The solvent versatility of the CSP was investigated for the enantioselective separation of four pairs of enantiomers using THF and chloroform as non-standard mobile phase eluent in HPLC. The influence of temperature on the resolution was investigated.

关键词： HPLC immobilized enantiomeric separation.

Activation of ERK1/2 in spinal cord contributes to the development of acute cystic pain in rabbits

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Neuroscience Bulletin 2006年第4期22卷 216-220页

作者： Yong-hong WANG li-Cai zhang Yin-Ming ZENG Key Laboratory of Anesthesiology of Jiangsu Province Xuzhou 221002 China

Objective To investigate the role of activated extracellular signal-regulated kinase 1/2 （ERK1/2） in spinal cord in the development of cystic pain in rabbit. Methods We observed the relationship between the activation of ERK1/2 in spinal cord and nociceptive behaviors, as well as the effect of U0126, a mitogen-activated protein kinase （MEK, upstream protein of ERK1/2） inhibitor, on cystic pain in rabbits by behavioral test, immunohistochemistry and western blot analysis. Results After injecting 0.5 ml formalin into gallbladder, the behaviors such as grasping of the cheek and licking of the abdomen increased in 30 min, with a significant increase in pERK1/2 expression in the spinal cord, as well as the pERK1/ 2 immunoreactive cells located in laminae Ⅴ-Ⅶ and X of the dorsal horn and ventral horn of T6 spinal cord. Administration of U0126 （100 -400 μg/kg body weight, i.v., 10 min before instillation of formalin） could attenuated nociceptive behaviors dose-dependently, but could not restrain the nociceptive behaviors completely even at the maximal efficient dose of 400 μg/kg body weight. Conclusion Activated ERK1/2 in the spinal cord at least partly participates in the development of acute inflammatory cystic pain induced by formalin in rabbits.

关键词： gallbladder cystic pain rabbit spinal cord pERK U0126

Tomographic imaging of P wave velocity structure beneath the region around Beijing

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Earthquake Science 2009年第4期22卷 403-408页

作者： Zhifeng Ding Xiaofeng Zhou Yan Wu Guiyin li hong zhang Institute of Geophysics China Earthquake Administration Beijing 100081 China

The three-dimension crustal and upper mantle structures in the region around Beijing were studied by seismic tomography. We used the P wave arrival times from local and teleseismic events. These events were recorded by 250 stations of the North China Seismic Array and 108 stations of the Beijing Telemetry Seismic Network. 118 869 P wave arrivals from 10 285 local events and 12 189 P wave arrivals from 107 teleseismic events were used in the inversion. We obtained the 3-D P wave velocity structure of the crust and upper mantle with the horizontal resolution of 0.3° in the studied region. The tomographic imaging shows the remarkably heterogeneous velocity variation. The velocity anomalies are in well agreement with the geological structure in the shallow crust. The different relationships between seismic activities and velocity anomalies may imply the different seismogenic structure and mechanism. Beneath the Moho under Taihangshan mountain and Yanshan mountain, we found the high velocity anomalies deep to 120 km and 200 km, respectively. The deep high velocity zone may be explained by the existence of the mountain root under Yanshan mountain. The high velocity anomalies in the upper mantle of North China basin may be the relics of the de-rooting from the former craton mantle lithosphere.

关键词： crustal and upper mantle structure Beijing seismic tomography

One new genus and three new species of wingless grasshoppers from Xizang,China(Orthoptera:Acridoidea)

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动物分类学报 2014年第2期39卷 263-268页

作者： Zhe-Min Zheng li-liang lin hong-li zhang Hui-Hua Zeng Institute of Zoology Shaanxi Normal University

One new genus and three new species of wingless grasshoppers, i.e, Pseudozubovskia gen. nov., Pseudozubovskia xizangensis sp. nov., Eokingdonella gongbugyanda Sla. nov., Dysanema magna sp. nov. are described. The type... 详细信息

关键词： Orthoptera Acridoidea wingless grasshopper new genus new species.