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Fermentation Performance and Characterization of Cold-Adapted Lipase Produced with Pseudomonas Lip35

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Agricultural Sciences in China 2009年第8期8卷 956-962页

作者： YU hong-wei han jun LI Ning QIE Xiao-sha JIAYing-min Institute of Food Science and Technology Agricultural University of Hebei Baoding 071001 PR.China

Strain of Pseudomonas Lip35 producing lipase was isolated in a refrigerator. Lipase production and characterization of this strain were investigated under different conditions. The Pseudomonas was cultivated in shaking flasks in a fermentation medium in various nutritional and physical environments. Lipase production has been influenced by the presence of yeast-extract, soybean powder, NaCI, and Tween-80. Maximum lipase productivity was obtained when the physical environment of the fermentation medium was optimal for 67 h. The production of lipase reached 58.9 U·mL^-1. The lipase of Pseudomonas Lip35 can be considered to be inducible, but the inducer had little influence on the production of lipase. The lipase was characterized and showed high lipolytic activity from pH 7.5-8.0. The optimum temperature was observed at 20℃ and the thermal inactivation of lipase was obvious at 60℃. The lipase activity was inhibited by K＋, stimulated by Ca^2＋, and thermostability decreased in the presence of Ca^2＋, therefore the lipase was Ca^2＋ -dependent cold-adapted enzyme.

关键词： cold-adapted lipase fermentation optimization lipase characterization Pseudomonas Lip35

Coal Gasification Wastewater Pretreatment with Coagulation and N_2 Flotation Combined System

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Journal of Harbin Institute of Technology(New Series) 2013年第5期20卷 20-24页

作者： hong-jun han Qian Zhao Chun-Yan Xu Hai-Feng Zhuang Peng Xu State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

A N 2 flotation tank combined with coagulation was developed as a pretreatment equipment of biological process to remove oil and other pollutants in coal gasification wastewater( CGW). With optimal PAC dosage of 20 mg / L,the sole coagulation process achieves removal efficiencies of 29. 34% and 26. 83% for oil and COD,respectively. For the sole N 2 floatation process,the optimal N 2 flux and HRT are 20 m3/ h and 20 min. Meanwhile,the oil and COD removal efficiencies are 35. 41% and 14. 26%,respectively. For the combined system of coagulation and N 2 floatation,the optimal parameter values are the same as the ones of separate processes. Correspondingly,the removal efficiencies are 46. 28% and 31. 89% for oil and COD, respectively. Besides,BOD 5 / COD of the effluent is improved. In contrast with conventional dissolved air floatation,the inert gas- N 2 prevents the formation of cyclopentenone, pyridine derivatives, and other heterocyclic aromatic hydrocarbons,thus improving the biodegradability of influent for the subsequent biological processes.

关键词： N2 flotation tank coagulation coal gasification wastewater oil biodegradability

Optimizing near-carbon-free nuclear energy systems:advances in reactor operation digital twin through hybrid machine learning algorithms for parameter identification and state estimation

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Nuclear Science and Techniques 2024年第8期35卷 177-203页

作者： Li‑Zhan hong He‑Lin Gong hong‑jun Ji Jia‑Liang Lu han Li Qing Li Paris Elite Institute of Technology Shanghai Jiao Tong UniversityShanghai 200240China Science and Technology on Reactor System Design Technology Laboratory Nuclear Power Institute of ChinaChengdu 610041China

Accurate and efficient online parameter identification and state estimation are crucial for leveraging digital twin simulations to optimize the operation of near-carbon-free nuclear energy *** previous studies,we developed a reactor operation digital twin(RODT).However,non-differentiabilities and discontinuities arise when employing machine learning-based surrogate forward models,challenging traditional gradient-based inverse methods and their *** study investigated deterministic and metaheuristic algorithms and developed hybrid algorithms to address these *** efficient modular RODT software framework that incorporates these methods into its post-evaluation module is presented for comprehensive *** methods were rigorously assessed based on convergence profiles,stability with respect to noise,and computational *** numerical results show that the hybrid KNNLHS algorithm excels in real-time online applications,balancing accuracy and efficiency with a prediction error rate of only 1%and processing times of less than 0.1 ***,algorithms such as FSA,DE,and ADE,although slightly slower(approximately 1 s),demonstrated higher accuracy with a 0.3%relative L_2 error,which advances RODT methodologies to harness machine learning and system modeling for improved reactor monitoring,systematic diagnosis of off-normal events,and lifetime management *** developed modular software and novel optimization methods presented offer pathways to realize the full potential of RODT for transforming energy engineering practices.

关键词： Parameter identification State estimation Reactor operation digital twin Reduced order model Inverse problem

Biodegradation Kinetics and Mechanism of a Typical Coal Gasification Pollutant:Quinoline

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Journal of Harbin Institute of Technology(New Series) 2014年第6期21卷 121-128页

作者： Peng Xu hong-jun han Sheng-Yong Jia Bao-Lin Hou Hai-Feng Zhuang Qian Zhao State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

A strain Pseudomonas ***1 was isolated from activated sludge and characterized by morphological observation,biochemical and physiological identification as well as 16 S rRNA gene sequence *** was a gram-negative,non-motile,rod-shaped with colonies which were white,opaque,small and wrinkled on solid *** strain was negative for catalase,gelatin liquefaction,M.R and V.P tests and was unable to reduce / restore *** 1406 bp 16 S rRNA gene fragment of PP1 was more than 99% identical to the Pseudomonas *** of quinoline as single substrate was conducted in batch experiments at different temperatures( 15- 40 ℃) and p H values( 5- 10).The results indicate that the optimum conditions for the degradation of quinoline by PP1 were 30 ℃ and p H *** ***1 was able to degrade 97.1%,95.4%,94.8%,63.6% and 40.4% quinoline when initial concentrations of quinoline were 50,100,200,300,and 400 mg / L,respectively,and lag phases were prolonged from 2 h to 10 *** maximum degradation rate( q) was obtained at an initial quinoline concentration( S0) of about 100 mg/L with q of *** experimentally obtained q values at various initial S0 were fitted by Haldane model,Yano model,Aiba model,Edward model and Webb model,and the results demonstrated that Haldane model gives the best fit for strain PP1 with coefficient of determination,R2= 0.9124 and SDavg= *** metabolic intermediates were identified by high performance liquid chromatograph( HPLC) and GC / ***,a possible pathway containing 5,6-dihydroxy-2-oxo-1,2-dihydroquinoline as an intermediate was proposed for the first time.

关键词： pseudomonas sp. quinoline biodegradation kinetics pathway

High-entropy oxide-supported platinum nanoparticles for efficient hydrogen evolution reaction

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Rare Metals 2024年第4期43卷 1537-1546页

作者： Ming-Yu Fan Jia-jun Wang jun Zhao hong Zhang Tian-Yi Ma Xiao-Peng han Wen-Bin Hu School of Materials Science and Engineering Tianjin Key Laboratory of Composite and Functional Materials Key Laboratory of Advanced Ceramics and Machining TechnologyTianjin UniversityTianjin300350China Joint School of National University of Singapore and Tianjin University International Campus of Tianjin UniversityFuzhou350207China School of Science RMIT UniversityMelbourneVIC3000Australia

High-entropy oxides(HEOs)are a new class of single-phase structures with unique electronic and catalytic properties;therefore,it is worthwhile to explore their applications in *** this study,a Pt/(FeCoNiCrAl)_(3)O_(4)nanohybrid using HEO as a support was developed as an efficient catalyst for the hydrogen evolution reaction(HER).Pt/(FeCoNiCrAl)_(3)O_(4)exhibited high HER activity with a low overpotential of 22 mV at10 mA·cm^(-2),outperforming other binary,ternary,and quaternary *** HER activity of Pt/(FeCoNiCrAl)_(3)O_(4)was higher than that of a commercial Pt/C with a significantly lower Pt *** catalyst exhibited good activity and long-term stability(60 h)in an electrolytic water-splitting *** good activity can be attributed to the fact that the introduction of Pt effectively facilitates electronic interactions between Pt and the *** addition,the HEO substrate was more favorable for dispersing Pt particles,optimizing the electrochemical specific surface area,and significantly reducing the charge resistance of the *** study extends the application of HEOs in electrocatalysis and demonstrates the promising prospects of HEOs as supports for electrocatalysts.

关键词： High-entropy oxides Hydrogen evolution reaction Nanohybrid Water electrolysis

A Multi-Stage Differential-Multifactorial Evolutionary Algorithm for Ingredient Optimization in the Copper Industry

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IEEE/CAA Journal of Automatica Sinica 2024年第10期11卷 2135-2153页

作者： Xuerui Zhang Zhongyang han jun Zhao School of Control Science and Engineering Dalian University of TechnologyDalian 116024China IEEE

Ingredient optimization plays a pivotal role in the copper industry,for which it is closely related to the concentrate utilization rate,stability of furnace conditions,and the quality of copper *** acquire a practical ingredient plan,which should exhibit long duration time with sufficient utilization and feeding stability for real applications,an ingredient plan optimization model is proposed in this study to effectively guarantee continuous production and stable furnace *** address the complex challenges posed by this integer programming model,including multiple coupling feeding stages,intricate constraints,and significant non-linearity,a multi-stage differential-multifactorial evolution algorithm is *** the proposed algorithm,the differential evolutionary(DE)algorithm is improved in three aspects to efficiently tackle challenges when optimizing the proposed ***,unlike traditional time-consuming serial approaches,the multifactorial evolutionary algorithm is utilized to optimize multiple complex models contained in the population of evolutionary algorithm caused by the feeding stability in a parallel ***,a repair algorithm is employed to adjust infeasible ingredient lists in a timely *** addition,a local search strategy taking feedback from the current optima and considering the different positions of global optimum is developed to avoiding premature convergence of the differential evolutionary ***,the simulation experiments considering different planning horizons using real data from the copper industry in China are conducted,which demonstrates the superiority of the proposed method on feeding duration and stability compared with other commonly deployed *** is practically helpful for reducing material cost as well as increasing production profit for the copper industry.

关键词： Copper industry differential-multifactorial evolution ingredient optimization multi-stage optimization

Advances in the study of mitophagy in osteoarthritis

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第3期25卷 197-211页

作者： hong CAO Xuchang ZHOU Bowen XU han HU Jianming GUO Miao WANG Nan LI jun ZOU Department of Sport Rehabilitation Shanghai University of SportShanghai 200438China National Key Laboratory of Immunity and Inflammation Naval Medical UniversityShanghai 200433China School of Sport Medicine and Rehabilitation Beijing Sport UniversityBeijing 100084China

Osteoarthritis(OA),characterized by cartilage degeneration,synovial inflammation,and subchondral bone remodeling,is among the most common musculoskeletal disorders globally in people over 60 years of *** initiation and progression of OA involves the abnormal metabolism of chondrocytes as an important pathogenic *** degeneration features mitochondrial dysfunction as one of the important causative factors of abnormal chondrocyte ***,maintaining mitochondrial homeostasis is an important strategy to mitigate *** is a vital process for autophagosomes to target,engulf,and remove damaged and dysfunctional mitochondria,thereby maintaining mitochondrial *** studies have revealed a strong association between mitophagy and OA,suggesting that the regulation of mitophagy may be a novel therapeutic direction for *** reviewing the literature on mitophagy and OA published in recent years,this paper elaborates the potential mechanism of mitophagy regulating OA,thus providing a theoretical basis for studies related to mitophagy to develop new treatment options for OA.

关键词： Mitophagy Osteoarthritis Chondrocyte Mitochondria Apoptosis

Advanced Sensors Towards Ubiquitous Power Internet of Things

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 871-890页

作者： Jinliang He Zhifei han jun Hu Department of Electrical Engineering Tsinghua UniversityBeijing 100084China IEEE CSEE

The ubiquitous power Internet of Things(UPIoT)uses modern information technology and advanced communication technologies to realize interconnection and human-computer interaction in all aspects of the power *** has the characteristics of comprehensive state perception and efficient information processing,and has broad application prospects for transformation of the energy *** fundamental facility of the UPIoT is the sensor-based information *** using advanced sensors,Wireless Sensor Networks(WSNs),and advanced data processing technologies,Internet of Things can be realized in the power *** this paper,a framework of WSNs based on advanced sensors towards UPIoT is *** addition,the most advanced sensors for UPIoT purposes are reviewed,along with an explanation of how the sensor data obtained in UPIoT is utilized in various scenarios.

关键词： Advanced sensors ubiquitous power Internet of Things wireless sensor networks

A Simple Proof of ACC for Minimal Log Discrepancies for Surfaces

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Acta Mathematica Sinica,English Series 2024年第2期40卷 425-434页

作者： Jing jun han Yu Jie LUO Shanghai Center for Mathematical Sciences Fudan UniversityJiangwan CampusShanghai 200438P.R.China Department of Mathematics Johns Hopkins UniversityBaltimoreMD 21218USA

Following Shokurov's idea,we give a simple proof of the ACC conjecture for minimal log discrepancies for surfaces.

关键词： Ascending chain condition minimal log discrepancies minimal model program surface