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“Warming yang and invigorating qi” acupuncture alters acetylcholine receptor expression in the neuromuscular junction of rats with experimental autoimmune myasthenia gravis

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Neural Regeneration Research 2016年第3期11卷 465-468页

作者： Hai-peng Huang hong Pan hong-feng wang School of Acupuncture and Moxibustion Changchun University of Chinese Medicine Changchun Jilin Province China Graduate School of Changchun University of Chinese Medicine Changchun Jilin Province China

Myasthenia gravis is an autoimmune disorder in which antibodies have been shown to form against the nicotinic acetylcholine nicotinic postsynaptic receptors located at the neuromuscular junction.＂Warming yang and invigorating qi＂ acupuncture treatment has been shown to reduce serum inflammatory cytokine expression and increase transforming growth factor beta expression in rats with experimental autoimmune myasthenia ***,few studies have addressed the effects of this type of acupuncture on the acetylcholine receptors at the neuromuscular ***,we used confocal laser scanning microscopy to examine the area and density of immunoreactivity for an antibody to the nicotinic acetylcholine receptor at the neuromuscular junction in the phrenic nerve of rats with experimental autoimmune myasthenia gravis following ＂warming yang and invigorating qi＂ acupuncture *** were inserted at acupressure points Shousanli（LI10）,Zusanli（ST36）,Pishu（BL20）,and Shenshu（BL23） once daily for 7 consecutive *** treatment was repeated after 1 day of *** found that area and the integrated optical density of the immunoreactivity for the acetylcholine receptor at the neuromuscular junction of the phrenic nerve was significantly increased following acupuncture *** outcome of the acupuncture therapy was similar to that of the cholinesterase inhibitor pyridostigmine *** findings suggest that ＂warming yang and invigorating qi＂ acupuncture treatment increases acetylcholine receptor expression at the neuromuscular junction in a rat model of autoimmune myasthenia gravis.

关键词： nerve regeneration myasthenia gravis acupuncture "Warming yang and invigorating qi" experimental autoimmune myasthenia gravis neuromuscular junction acetylcholine receptor neural regeneration

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Three new C_(20)-diterpenoid alkaloids from Delphinium anthriscifolium ***

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Chinese Chemical Letters 2009年第6期20卷 698-701页

作者： Xiao Yu Liu Qiao hong Chen feng Peng wang Department of Chemistry of Medicinal Natural Products West China College of Pharmacy Sichuan University Chengdu 610041 China

Three new C20-diterpenoid alkaloids, designated as anthriscifolmines A-C （1-3）, together with two known alkaloids denudatine and delgramine, were isolated from the whole herb of Delphinium anthriscifolium var. savat... 详细信息

关键词： Delphinium anthriscifolium vat. savatieri C20-diterpenoid alkaloid Anthriscifolmines A-C

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Diversity analysis of soil dematiaceous hyphomycetes from the Yellow River source area:Ⅰ

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 829-834页

作者： Hao-qin PAN Jin-feng YU Yue-ming WU Tian-yu ZHANG hong-feng wang Mycological Laboratory Department of PlantPathology Shandong Agricultural University Tai＇an 271018 China

Twenty-four soil samples of eight ecosystem-types around the Yellow River source area were investigated for the number and specific composition of soil dematiaceous hyphomycetes by dilution plate technique. And then the co-relationship between genus species of soil dematiaceous hyphomycetes and ecosystem-types was analyzed. The results show that the amount and species distribution of soil dematiaceous hyphomycetes had an obvious variability in different ecosystem-types, and that the dominant genus species varied in the eight ecosystem-types studied, with Cladosporium being the dominant genus in seven of the eight ecosystem-types except wetland. The index of species diversity varied in different ecosystem-types. The niche breadth analysis showed that Cladosporium had the highest niche breadth and distributed in all ecosystem-types, while the genera with a narrow niche breadth distributed only in a few ecosystem-types. The results of niche overlap index analysis indicated that Stachybotrys and Torula, Doratomyces and Scolecobasidium, Cladosporium and Chrysosporium had a higher niche overlap, whereas Arthrinium and Gliomastix, Phialophora and Doratomyces, Oidiodendron and Ulocladium had no niche overlap.

关键词： Yellow River source area Ecosystems Fungal species diversity Soil dematiaceous hyphomycetes Niche breadth Niche overlap

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Radio frequency interference mitigation using pseudoinverse learning autoencoders

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Research in Astronomy and Astrophysics 2020年第8期20卷 121-128页

作者： hong-feng wang Mao Yuan Qian Yin Ping Guo Wei-Wei Zhu Di Li Si-Bo feng Image Processing and Pattern Recognition Laboratory School of Artificial IntelligenceBeijing Normal UniversityBeijing 100875China CAS Key Laboratory of FAST National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China School of Information Management Dezhou UniversityDezhou 253023China Image Processing and Pattern Recognition Laboratory School of System ScienceBeijing Normal UniversityBeijing 100875China Institute for Astronomical Science Dezhou UniversityDezhou 253023China University of Chinese Academy of Sciences Beijing 100049China Hanvon Technology Co. LtdBeijing 100193China NAOC-UKZN Computational Astrophysics Centre University of KwaZulu-NatalDurban 4000South Africa

Radio frequency interference(RFI)is an important challenge in radio *** comes from various sources and increasingly impacts astronomical observation as telescopes become more *** this study,we propose a fast and effective method for removing RFI in pulsar *** use pseudo-inverse learning to train a single hidden layer auto-encoder(AE).We demonstrate that the AE can quickly learn the RFI signatures and then remove them from fast-sampled spectra,leaving real pulsar *** method has the advantage over traditional threshold-based filter method in that it does not completely remove contaminated channels,which could also contain useful astronomical information.

关键词： pulsars:general methods:numerical methods:data analysis

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Female moths of cotton bollworm （Lepidoptera： Noctuidae） captured by waterbasin traps baited with synthetic female sex pheromone

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Insect Science 2006年第4期13卷 293-299页

作者： JIAN-WEI SU hong-TUO wang feng GE State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology CAS Beijing China

Cotton bollworm, Helicoverpa armigera （Hüibner）（Lepidoptera： Noctuidae）, is one of the most important pest insects in cotton fields in China. Female moths were captured by waterbasin traps with a synthetic female sex pheromone blend in cotton fields over three years. The blend contained （Z）-11-hexadecenal and （Z）-9-hexadecenal with a ratio of 97：3. Each pheromone dispenser was impregnated with 2.0 mg of pheromone blend and 0.2 mg of antioxidant dissolved with 0.1 mL of hexane, and there was a control dispenser with a similar amount of antioxidant and solvent only. Waterbasin traps were deployed in three configurations in the fields. ‘A＇ was pheromone traps only, ‘B＇ was both pheromone and control traps, ‘C＇ was control traps only. （i） In four plots of ‘A＇, the average weekly female catch was 1.5, and more females were captured by centrally located pheromone traps. （ii） In three plots of‘B＇, control traps also captured female as well as male moths, but average weekly female catches of control traps was significantly lower than that in pheromone-baited traps. （iii） There were significant linear relationships between the average weekly female catch and the corresponding layer in pheromone-baited traps in both ‘A＇ and ‘B＇ plots, and in quadratic equations in control in ‘B＇ plots. （iv） With the increase of the interval of traps, average weekly female catches per trap increased but average weekly female catches per hectare decreased. （v） Among the female moths captured by pheromone traps, 88.3% were mated female moths which each containing 1.46 spermatophores, while in control traps 86.9% of the mated female moths had 0.90 spermatophores. There was a significant difference between the average numbers of spermatophores of mated females in pheromone traps and in controls.

关键词： female sex pheromone Helicoverpa armigera mass trapping spatial distribution

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CGI-100 Specific shRNA Inhibits Proliferation and Induces Differentiation in Leukemia K562 Cells

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Chinese Journal of Cancer Research 2010年第2期22卷 126-134页

作者： Yong-qiang wang Ting-mei Chen Yan Lei hong-lei feng Ke wang Yan Zhang The Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education Department of Clinical Biochemistry Chongqing Medical University Chongqing 400016 China

Objective： To investigate the characteristics of CGI-100- knockdown K562 cells and the effect of CGI-100 RNA interference （RNAi） on matrine-treated K562 cells. Methods： Three oligonucleotides targeting CGI-100 gene and a pair of negative control containing the same nucleotide composition with a different sequence were devised and chemically synthesized. The inhibition efficiency of CGI-100 expression by shRNA-CGI-100 in K562 cells was determined using semiquantitative RT-PCR and dot blot hybridization. The effect of CGI-100 RNAi on the growth of K562 cells was examined using MTT assay and cell differentiation was measured by distinct approaches including flow cytometry, benzidine staining and electron microscope. After CGI-100-konckdown K562 cells were incubated with 0.2 mg/ml of matrine or 30 Ixmol/L of hemin for 48 h, the expression levels of Glycophorin A（GPA）（CD235a） and Growth factor independence-lB mRNA（Gfi-IB mRNA） were measured by RT-PCR and the protein levels of GPA, CD14 and CD15 were detected by flow cytometry. Results： The eukaryotic expression vectors of CGI-100 RNAi were successfully constructed. The K562/shRNA-CGI-100 cell line was established in which the inhibition efficiency of CGI-100 gene expression by shRNA-CGI-100 was 54%. CGI-100-knockdown inhibited the proliferation and induced erythroid differentiation in K562 cells. Compared with the control K562 cells, the K562/shRNA-CGI-100 cells showed decreased absorbance value detected by MTT assay, decreased enchromation, increased heterochromation, increased percentage of G0/Gx phase cells, decreased population of S phase cells, decreased PI （proliferation index of cells）, and elevated percentage of benzidine-positive cells. Moreover, the sensitivity of K562/shRNA-CGI-100 cells to either matrine or hemin was enhanced and the sensitivity of these cells to matrine was higher than that to hemin. Compared with the control K562 cells, matrine treatment in K562/shRNA-CGI-100 cells resulted in increased i

关键词： CGI-100 RNA interference Leukemia Erythroid differentiation Matrine

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In Situ Synthesis of Heat Resistant Gradient Composite on Steel Surface

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Journal of Iron and Steel Research International 2009年第2期16卷 89-94页

作者： CUI Xiang-hong wang feng wang Shu-qi YANG Zi-run GAO Ming-juan WEI Min-xian School of Materials Science and Engineering Jiangsu University Zhenjiang 212013 Jiangsu China

A heat resistant gradient composite was synthesized in situ on steel with the self-propagating high temperature synthesis （SHS） reaction of 3Ni-Al-Ti-C system during casting. The phases, microstructure, and composition of the composite were analyzed by using an X-ray diffractometer （XRD）, and a scanning electron microscope （SEM） coupled with an energy-dispersive X-ray spectroscope （EDS）. The formation mechanism of the composite is also discussed. TiC/Ni3 Al/steel gradient composite is achieved by forming the gradient distributions of Fe, Ni, and Al, accompanied with the gradient variation of the microstructure from TiC/Nia A1, to TiC/Ni3 Al/steel, and to steel. The composite is in situ synthesized through whole reaction of 3Ni-Al-Ti-C system in liquid steel and densification procedure, and the liquid steel infiltrates into pores in the SHS product and forces liquid Ni3Al to form self-compaction further.

关键词： steel heat resistant composite synthesis microstructure

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Identification of thermal effects involved in DSC experiment on Al-Cu-Mg-Ag alloys with high Cu:Mg ratio

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International Journal of Minerals,Metallurgy and Materials 2011年第6期18卷 671-675页

作者： Jian-bo Zhang Yong-an Zhang Bao-hong Zhu feng wang Zhi-hui Li Xi-wu Li Bai-qing Xiong State Key Laboratory of Nonferrous Metals and Processes General Research Institute for Non-ferrous Metals Beijing 100088 China

Precipitation reactions in the differential scanning calorimetry （DSC） of an Al-Cu-Mg-Ag alloy were identified by analyzing the results from hardness test,electrical conductivity test,and transmission electron microscope （TEM） *** is discovered that thermal effects can be identified through selected area electron diffraction and bright-field *** reaction peaks around 171,231,and 276℃ can be attributed to a structural rearrangement of coherent zones,to the precipitation of Ω phases,and to the precipitation of Ω and θ＇ and possible combination with the transition of θ＇→θ,*** addition,the hardness and electrical conductivity of the alloy change proportionately with the progression of reactions during the heating *** phenomenon can be attributed to the evolution of the micro-structure.

关键词： aluminum alloys thermal effects identification differential scanning calorimetry microstructure

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A deep learning approach for detecting candidates of supernova remnants

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Research in Astronomy and Astrophysics 2019年第3期19卷 85-96页

作者： Wei Liu Cong Dai Meng Zhu Bing-Yi wang Kang Wu Xian-Chuan Yu Wen-Wu Tian Meng-Fei Zhang hong-feng wang College of Information Science and Technology Beijing Normal University National Astronomical Observatories Chinese Academy of Sciences School of Information Management Dezhou University

Detecting supernova remnant(SNR) candidates in the interstellar medium is a challenging task because SNRs have weak radio signals and irregular shapes. The use of a convolutional neural network is a deep learning method that can help us extract various features from images. To extract SNRs from astronomical images and estimate the positions of SNR candidates, we design the SNR-Net model composed of a training component and a detection component. In addition, transfer learning is used to initialize the network parameters, which improves the speed and accuracy of network training. We apply a T-T plot(of the different brightness temperatures of map pixels at two different frequencies) to calculate the spectral index of SNR candidates. To accelerate the scientific computing process, we take advantage of innovative hardware architecture, such as deep learning optimized graphics processing units, which increases the speed of computation by a factor of 5. A case study suggests that SNR-Net may be applicable to detecting extended sources in the images automatically.

关键词： methods data analysis-techniques image processing-stars fundamental parameters

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INFLUENCE OF “WARMING YANG AND NOURISHING QI” ACUPUNCTURE TREATMENT ON SERUM IL-12 AND IL-18 EXPRESSION LEVELS IN RATS WITH EXPERIMENTAL AUTOIMMUNE MYASTHENIA GRAVIS AND ITS MECHANISM

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Journal of Integrative Medicine 2014年第3期 238-239页

作者： hong-feng wang Graduate School Changchun University of Chinese Medicine

Objective: To observe the influence of the "Warming yang and Nourishing qi" acupuncture treatment on the serum interleukin-12(IL-12) and interleukin-18(IL-18) expression levels in rats with experimental autoimmune myasthenia gravis(EAMG) and to analyze the mechanism of acupuncture in treatment of myasthenia gravis(MG).Methods: AchRα1 129-145 peptide fragments were used to immune the female Lewis rats to establish EAMG rat *** EAMG model rats were randomly divided into acupuncture treatment group, drug control group and model control group(n=10); the unmodeled 10 rats purchased at the same period were used as the blank control *** rats in acupuncture treatment group were treated by "Warming yang and Nourishing qi" acupuncture treatment, 30 min, once a day, 7 d for a period, 1 d rest between two periods, and two periods of continuous *** rats in drug control group were treated by the pyridostigmine lavaged treatment, 18.5 mg/(kg·d), continuous treatment for 15 *** rats in the other two groups had no any special *** treatment, the blood samples from the vein of tail root of the rats in 4 groups were collected, and the serum expression levels of IL-12 and IL-18 were detected with ELISA ***: After treatment, compared with the model control group, the serum IL-12 and IL-18 expression levels of the EAMG rats in the acupuncture treatment group and the drugcontrol group were significantly decreased(P0.05).Conclusion: "Warming yang and Nourishing qi" acupuncture treatment can reduce the serum IL-12 and IL-18 expression levels of EAMG *** the treatment of MG, the effect of acupuncture was similar to pyridostigmine.

关键词： IL MG WARMING YANG AND NOURISHING QI ACUPUNCTURE TREATMENT ON SERUM IL-12 AND IL-18 EXPRESSION LEVELS IN RATS WITH EXPERIMENTAL AUTOIMMUNE MYASTHENIA GRAVIS AND ITS MECHANISM INFLUENCE OF

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