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Kangfuxin solution alleviates esophageal stenosis after endoscopic submucosal dissection:A natural ingredient strategy

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World Journal of Gastroenterology 2025年第1期31卷 110-125页

作者： Xin Zhou Dan Ma Yi-Xiang He Jing Jin hong-lian Wang Yun-Feng Wang Fan Yang Jian-Qin Liu Jie Chen Zhi Li Department of Spleen and Stomach Diseases the Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina The Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Digestive System Diseases of Luzhou City The Affiliated Traditional Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina Department of Gastroenterology Changhai HospitalNaval Medical UniversityShanghai 20082China Research Center of Integrated Chinese and Western Medicine The Affiliated Traditional Chinese Medicine HospitalSouthwest Medical UniversityLuzhou 646000Sichuan ProvinceChina School of Integrated Traditional Chinese and Western Clinical Medicine North Sichuan Medical CollegeNanchong637100Sichuan ProvinceChina

BACKGROUND Esophageal stricture ranks among the most significant complications following endoscopic submucosal dissection(ESD).Excessive fibrotic repair is a typical pathological feature leading to stenosis after *** To examine the effectiveness and underlying mechanism of Kangfuxin solution(KFX)in mitigating excessive fibrotic repair of the esophagus *** Pigs received KFX at 0.74 mL/kg/d for 21 days after esophageal full circumferential *** examinations occurred on days 7 and 21 *** vitro,recombinant transforming growth factor(TGF)-β1(5 ng/mL)induced a fibrotic microenvironment in primary esophageal fibroblasts(pEsF).After 24 hours of KFX treatment(at 1.5%,1%,and 0.5%),expression ofα-smooth muscle actin-2(ACTA2),fibronectin(FN),and type collagen I was *** signaling was analyzed,including TGF-β1,Smad2/3,and phosphor-smad2/3(p-Smad2/3).RESULTS Compared to the Control group,the groups treated with KFX and prednisolone exhibited reduced esophageal stenosis,lower weight loss rates,and improved food tolerance 21 d after *** treatment,Masson staining revealed thinner and less dense collagen fibers in the submucosal ***,the expression of fibrotic effector molecules was notably ***,KFX downregulated the transduction levels of fibrotic functional molecules such as TGF-β1,Smad2/3,and p-Smad2/*** vitro,pEsF exposed to TGF-β1-induced fibrotic microenvironment displayed increased fibrotic activity,which was reversed by KFX treatment,leading to reduced activation of ACTA2,FN,and collagen *** 1.5%KFX treatment group showed decreased expression of p-Smad 2/3 in TGF-β1-activated *** KFX showed promise as a therapeutic option for post-full circumferential esophageal ESD strictures,potentially by suppressing fibroblast fibrotic activity through modulation of the TGF-β1/Smads signaling pathway.

关键词： Kangfuxin solution Natural component Endoscopic submucosal dissection Esophagus stricture Fibrosis

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三七总皂苷通过EGFR/HSP27轴影响子宫内膜癌细胞和巨噬细胞的交互作用

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中国药理学通报 2025年第2期41卷 306-315页

作者：雷燕冯春邢琦高悦廉红梅杜欣湖北省妇幼保健院妇科湖北武汉430070

目的探讨三七总皂苷(Panax notoginseng saponins,PNS)调控表皮生长因子受体(epidermal growth factor receptor,EGFR)/热休克蛋白27(heat shock protein 27,HSP27)轴对子宫内膜癌(endometrial cancer,EC)细胞和巨噬细胞交互作用的影响。方法将ishikawa细胞分为:Control组、DDP组、PNS给药组、M2-CM组、M2-CM+DDP组、M2-CM+PNS组、M2-CM+PNS+oe-NC组、M2-CM+PNS+oe-EGFR组、PNS(200 mg·L^(-1))+oe-NC、PNS(200 mg·L^(-1))+oe-EGFR、oe-NC组、oe-EGFR组、oe-EGFR+si-NC组和oe-EGFR+si-HSP27组。MTT检测细胞增殖,Transwell实验检测细胞侵袭,TUNEL检测细胞凋亡,qRT-PCR检测巨噬细胞极化标志物CD86、CD163的mRNA水平,ELISA检测iNOS、IL-12水平。Western-blot检测EGFR和HSP27蛋白表达。构建裸鼠移植瘤模型并用PNS治疗裸鼠,评估PNS在体内的作用。结果与Control组相比,PNS与DDP明显抑制ishikawa细胞增殖与侵袭,诱导其凋亡。M2-CM处理抑制M1型巨噬细胞标志物,促进M2型巨噬细胞标志物,诱导ishikawa细胞生长,但该作用被PNS处理逆转。EGFR被证实为PNS的靶点,且相对于M2-CM+PNS+oe-NC组,EGFR过表达促进M2型巨噬细胞标志物水平,诱导ishikawa细胞增殖与侵袭,并抑制细胞凋亡。敲减HSP27能够逆转EGFR过表达对ishikawa细胞生物学行为的影响。动物实验显示,PNS能够抑制肿瘤生长,减少肿瘤组织中CD163、EGFR和HSP27阳性表达。结论PNS通过调控EGFR/HSP27轴影响巨噬细胞和EC细胞互作用进而参与EC进展。

关键词：三七总皂苷子宫内膜癌细胞增殖热休克蛋白27 表皮生长因子受体巨噬细胞

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分离富集磷酸化/糖基化蛋白质/肽的磁固相萃取新材料

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分析测试学报 2025年第1期44卷 1-11页

作者：鲁艳张森张锋葛鸿延庄琬月乔俊琴练鸿振生命分析化学国家重点实验室南京大学化学化工学院南京大学现代分析中心江苏南京210023

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其... 详细信息

蛋白质磷酸化和糖基化是蛋白质两种主要的翻译后修饰过程。磷酸化和糖基化蛋白质在细胞的各个生命周期内起着关键的作用,对于临床疾病的早期诊断等也具有重要的意义。但由于它们在实际生物样品中含量低、基质干扰大,选择合适的方法对其进行分离富集成为蛋白质组学研究中决定性的步骤之一。磁固相萃取(MSPE)是以磁性纳米颗粒为吸附剂的固相萃取技术(SPE),较之常规SPE具有操作简单、环境友好、材料易于回收利用等优势,因而被广泛应用于磷酸化和糖基化蛋白/肽段等分析前的分离富集。该文对近5年文献中用于磷酸化和糖基化蛋白质分离富集的MSPE材料进行系统的归纳和评述,并对此类分离富集材料的未来发展方向进行了展望和探讨。

关键词：磷酸化蛋白质糖基化蛋白质磁固相萃取磁性纳米颗粒

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On the Structure of Quantum Toroidal Superalgebraε_(m|n)

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Acta Mathematica Sinica,English Series 2023年第11期39卷 2117-2138页

作者： Xiang Hua WU hong Da LIN hong lian ZHANG Department of Mathematics Shanghai UniversityShanghai 200444P.R.China Newtouch Center for Mathematics of Shanghai University Shanghai 200444P.R.China

Recently the quantum toroidal superalgebraε_(m|n)associated with gl_(m|n)was introduced by *** and ***,which is not a quantum Kac-Moody *** quantum toroidal superalgebraε_(m|n)exploits infinite sequences of generators and relations of the form,which are called Drinfeld *** this paper,we use only finite set of generators and relations to define an associative algebraε'_(m|n)and show that there exists an epimorphism fromε'_(m|n)to the quantum toroidal superalgebraε_(m|n).In particular,the structure ofε'_(m|n)enjoys some properties like Drinfeld-Jimbo realization.

关键词： Quantum toroidal superalgebra Drinfeld realization Lie superalgebra

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Furazolidone,amoxicillin,bismuth and rabeprazole quadruple rescue therapy for the eradication of Helicobacter pylori

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World Journal of Gastroenterology 2009年第7期15卷 860-864页

作者： hong Cheng Fu-lian Hu Department of Gastroenterology Peking University First Hospital Beijing 100034 China

AIM： To compare the efficacy and side effect profiles of three furazolidone and amoxicillin-based quadruple rescue therapies for the eradication of Helicobacter pylori （H pylonS. METHODS： Patients who failed in the Hpylori eradication therapy for at least one course were randomly allocated into three groups. Group A received rebaprazole 10 mg＋ amoxicillin 1 g ＋ furazolidone 100 mg, and bismuth subcitrate 220 mg, twice daily for 1 wk; group B received the same regimen of group A but for 2 wk; and group C received the same regimen of group B, but furazolidone was replaced by furazolidone 100 mg three times daily. To record the side effect profiles at the end of the treatment, Hpylori eradication was assessed with 13C-urea breath test 4 wk after therapy. RESULTS： Sixty patients were enrolled including 28 males, and 20 patients in each group. The average age of the patients was 49.2 years, ranging from 18 to 84 years. H pylori eradication rates with per-protocol analysis were 82%, 89% and 90% in the three groups, respectively. Side effects were found in 11 patients, including mild dizziness, nausea, diarrhea and increased bowel movement. None of the 11 patients needed treatment for their side effects. CONCLUSION： One- or two-week furazolidone and amoxicillin-based quadruple rescue therapy with a low dose furazolidone （100 mg bid） for the eradication of Hpylori is effective. Extending the antibiotic course to 14 d could improve the eradication rates.

关键词： Helicobacter pylori Rescue therapy Quadruple therapy Furazolidone Low dose

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Establishment of Real-Time TaqMan-Fluorescence Quantitative RT-PCR Assay for Detection and Quantification of Porcine Lipoprotein Lipase mRNA

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Agricultural Sciences in China 2009年第10期8卷 1256-1262页

作者： lian hong-xia LU De-xun GAO Min College of Animal Science and Veterinary Medicine Henan Agricultural University Zhengzhou 450002 P.R. China Institute of Animal Nutrition Inner Mongolian Academy of Agricultural and Animal Sciences Huhhot 010031 P.R. China

Porcine lipoprotein lipase （LPL） cDNA was cloned as the standard for real-time quantifying LPL mRNA and the TaqMan-fluorescence quantitative PCR assay for detection was established. The total RNA extracted from Longissimus dorsi of porcine was reverse-transcribed to cDNA. LPL cDNA was ligated with pGM-T vector and transformed into Escherichia coli TOP10. Plasmid DNA extracted from positive clones was verified by PCR amplification and sequenced. LPL was amplified by real-time fluorescence quantitative PCR from the plasmid DNA. The concentration of DNA template purified was detected by analyzing absorbance in 260 nm and then the combined plasmid was diluted to series as standard for fluorescence quantitative PCR （FQ-PCR）. The method of LPL mRNA real-time PCR was well established, which detected as low as 103 with the linear range 10^3 to 10^10 copies. The standard curves showed high correlations （R2 = 0.9871）. A series of standards for real-time PCR analysis have been constructed successfially, and real-time TaqMan-fluorescence quantitative RT-PCR is reliable to quantitatively evaluate FQ-PCR mRNA in L. dorsi of porcine.

关键词： porcine lipoprotein lipase FQ-PCR TaqMan fluorogenic probe

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International Sports Biomechanics Research Trend

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中国体育科技 2009年第1期45卷 26-28页

作者： hong You-lian The Chinese University of hong Kong Hong Kong China

The Trend The trend of current international sports biomechanics research is evident in the papers presented in the recent annual International Conferences on Biomechanics in Sports.

关键词：运动生物力学体育运动锻炼方法体育设施培训方法

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Elevation,bedrock exposure,land use,interbedded limestone and clastic rock,and vegetation coverage dominate the spatiotemporal variability of soil erosion in karst basin

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Journal of Mountain Science 2023年第9期20卷 2519-2535页

作者： CHEN Mei GAO Jia-yong CHEN hong-lian JING Jun LI Rui School of Karst Science Guizhou Normal UniversityGuiyang 550001China State Engineering Technology Institute for Karst Desertification Control Guiyang 550001China

Soil erosion is a prominent environmental problem in karst *** the spatiotemporal variability of soil erosion and the factors that influence soil erosion is of great significance for regional soil erosion prevention and ***,the mechanisms influencing the characteristic features of the karst basins,such as bedrock exposure and lithology,still need to be further *** study used GIS technology,the Revised Universal Soil Loss Equation model,Getis–Ord Gi*,and partial least squares regression(PLSR)to identify the dominant factors influencing soil erosion and the spatiotemporal variability of soil erosion in 31 sub-basins of the Dabang River Basin(DRB),a typical karst area of Southwest China,from 2010 to *** results indicated that soil erosion in the DRB from 2010 to 2020 was generally decreasing,the mean soil erosion in the DRB in 2010,2015 and 2020 was 18.46,16.51 and 15.29 t ha^(-1)a^(-1),*** the study period,the area of slight erosion increased by 26.39%(706.54 km^(2)),while severe erosion enlarged by 26.36 km^(2).Spatially,the DRB was primarily affected by medium and slight soil *** hot spot areas of soil erosion(key control areas)were mainly concentrated in the central and southern parts of the basin,decreasing each year,and the area of soil erosion hot pots has decreased from 43.22%to 20.60%.PLSR decoupling results show that elevation,bedrock exposure,land use type,interbedded limestone and clastic rock,and vegetation coverage were identified as the key variables affecting soil erosion,explaining 52.8%of soil erosion variability,with a high value of the Variable Importance on Projection(VIP)more than *** results can be used as a reference for comprehensive control of soil erosion and water loss in the basin.

关键词： Soil erosion Karst basin RUSLE model Spatiotemporal variability PLSR

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Luminescent property and energy transfer from Ce3＊ to Tb3＋ in YAI3（BO3）4

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Optoelectronics Letters 2013年第3期9卷 194-197页

作者： DAI Xiu-hong LI hong-lian PANG Li-bin GAO Shao-jie College of Physics Science and Technology Hebei University Baoding 071002 China College of Quality & Technical Supervision Hebei University Baoding 071002 China Department of Foreign Language Teaching and Research Hebei University Baoding 071002 China

A series of Ce3＋, Tb3＋ or Ce3＋/Tb3＋ doped YA13（BO3）4 phosphors are synthesized by a high temperature solid-state reaction, and their luminescent properties are investigated. YA13（BO3）a：Ce3＋ shows a broad emission band at 422 nm under the 367 nm radiation excitation. YA13（BO3）4：Tb3＋ can be efficiently excited by the ultraviolet （UV） light, and produces green emission. The emission intensity of YA13（BO3）4：Tb3＋ can be enhanced by adjusting Tb3＋ doped content, and reaches the maximum at 0.06 mol Tb3＋. When Ce3＋ is codoped, the emission intensity of Tb3＋ in YA13（BO3）4 can be enhanced, but the commission international del＇eclairage （CIE） chromaticity coordinates of YA13（BO3）4：Tb3＋ have almost no change. Moreover, the energy transfer from Ce3＋ to Tb3＋ in YA13（BO3）4 is studied.

关键词：能量转移发光性能固相反应合成绿光发射发光强度铽掺杂发光特性掺杂含量

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Characteristics of an Indium-Rich InGaN p-n Junction Grown on a Strain-Relaxed InGaN Buffer Layer

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Chinese Physics Letters 2013年第4期30卷 174-176页

作者： YANG lian-hong ZHANG Bao-Hua GUO Fu-Qiang Xinjiang Laboratory of Phase Transitions and Microstructures of Condensed Matter Yili Normal UniversityYining 835000 Department of Physics Changji CollegeChangji 831100

An indium-rich InGaN p-n junction is grown on a strain-relaxed InGaN buffer *** results show that the n-InGaN is grown coherently on the buffer layer but the p-InGaN layer exhibits a partial strain *** fabricated InGaN p-n junction has a low reverse leakage current density on the order of 10^(-8)A/cm^(2)within the measured voltage range,and exhibits a wide spectral response due to the presence of band tail states or deep level states which origin from indium composition fluctuation or various *** measured peak responsivity at 438 nm is 31 mA/W at zero bias and reaches 118 mA/W at 3 V reverse *** addition,the Raman spectra of the p-and n-type InGaN alloys are also analyzed.

关键词： InGaN relaxed indium

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