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A Study of Protein and Glycoprotein Syntheses in Pre-implantation Mouse Embryos Using Mini-2D-Electrophoresis, Video Densitometer Scanning and Computer-Image Analysis

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Science China Chemistry 1993年第1期36卷 39-44页

作者：郑明曹詠清 B.LoftsDepartment of Zoology University of hong kong hong kong 陈廷汉 Department of Endocrinology and Laboratory of Reproductive Biology Institute of Zoology Academia Sinica Beijing 100080 PRC Department of Zoology University of Hong Kong Hong Kong

In the present study, proteins and glycoproteins of mouse embryos at 2-cell,morula and blastocyst stages were *** techniques of ^(35)S-Met incorporation,ConA antiserum-precipitating ConA-binding proteins, mini-2D-electrophoresis, fluorography,video densitometer scanning and the computer-lmage system were used for *** of the investigations indicated that proteins and glycoproteins were synthesized by the embryos in a gradual increase manner from 2-cell to blastocyst. A relatively large amount of glycoproteins was synthesized during the morula and blastocyst *** specific small glycoproteins respectively with molecular weights about 6500 and 9000 and PIs at 5.0 and 6.5 were apparently synthesized in the blastocyst but not in the 2-cell or the morula.

关键词： implantation mouse embryos glyeoproteins mini 2D-electrophoresis computer-image analysis.

Reaction of [η~5:σ-Me_2C(C_5H_4)(C_2B_(10)H_(10))]Ru(NCCH_3)_2 with internal alkynes:Synthesis and structural characterization of ruthenium-cyclobutadiene and ruthenacyclopentatriene complexes

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Science China Chemistry 2010年第10期53卷 2123-2128页

作者： QIU ZaoZao, SUN Yi & XIE ZuoWei Department of Chemistry, The Chinese University of hong kong, Shatin, New Territories, hong kong, China Department of Chemistry The Chinese University of Hong Kong Hong Kong China

Reaction of [η5:σ-Me2C(C5H4)(C2B10H10)]Ru(NCCH3)2 (1) with R1C≡CR1(R1 = Et, Ph) in toluene at 80 °C yielded organoruthenium cyclobutadiene complexes [η5:σ-Me2C(C5H4)(C2B10H10)]Ru(-4-C4R14) in >80% yield. Treatment of 1 with diynes R2C≡C(CH2)3C≡CR2 (R2 = Me, Et) in toluene at room temperature yielded ruthenacyclopentatrienes [η5:σ-Me2C (C5H4)(C2B10H10)]Ru[=C2(R2)2C2(CH2)3] in >85% yield. These new complexes were fully characterized by various spectroscopic techniques, elemental analyses and single-crystal X-ray diffraction studies. The possible reaction mechanism was proposed.

关键词： alkyne carborane diyne ruthenacyclopentatriene ruthenium ruthenium-cyclobutadiene

Cytogenetic and molecular genetic alterations in hepatocellular carcinoma

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Acta Pharmacologica Sinica 2005年第6期26卷 659-665页

作者： Sze-hang LAU, Xin-yuan GUAN~1 Department of Clinical Oncology, Faculty of Medicine, The University of hong kong, hong kong, China Department of Clinical Oncology Faculty of Medicine The University of Hong Kong Hong Kong China

Specific chromosome aberrations are frequently detected during the development of hepatocellular carcinoma. Molecular cytogenetic approaches such as comparative genomic hybridization and loss of heterozygosity analyses have provided fruitful information on changes in HCC cases at the genomic level. Mapping of chromosome gains and losses have frequently resulted in the identification of oncogenes and tumor suppressors, respectively. In this review, we summarize some frequently detected chromosomal aberrations reported for hepatocellular carcinoma cases using comparative genomic hybridization and loss of heterozygosity studies. Focus will be on gains of lq, 8q, and 20q, and losses of 4q, 8p, 13q, 16q, and 17p. We then examine the candidate oncogenes and tumor suppressors located within these regions, and explore their possible functions in hepatocarcinogenesis. Finally, the impact of microarray-based screening platforms will be discussed.

关键词： hepatocellular carcinoma chromosome aberrations oncogenes tumor suppressor genes

＂Sichuan Earthquake Community-based Rehabilitation Resource Center＂ Project Summary

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中国康复医学杂志 2013年第12期28卷 1083-1084页

作者： The hong kong Society for Rehabilitation International and China Division The hong kong Society for Rehabilitation International and China Division

The Sichuan Earthquake Community-based Rehabilitation Resource Center('the Project')was funded by the Trust Fund in Support of Reconstruction in the Sichuan Earthquake Stricken Areas of the Government of the hong kong Special Administrative Region for a period of 36 *** co-operation with Sichuan’s West China Hospital,the hong kong Society for Rehabilitation has started to set up community-based rehabilitation network points and to implement community-based

关键词：康复医学社会康复医学康复教育康复

A Computer-Aided FPS-Oriented Approach for Construction Briefing

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Tsinghua Science and Technology 2008年第S1期13卷 292-297页

作者： Xiaochun Luo, Qiping Shen Department of Building and Real Estate, the hong kong Polytechnic University, hong kong, China Department of Building and Real Estate the Hong Kong Polytechnic University Hong Kong China

Function performance specification (FPS) is one of the value management (VM) techniques developed for the explicit statement of optimum product definition. This technique is widely used in software engineering and manufacturing industry, and proved to be successful to perform product defining tasks. This paper describes an FPS-oriented approach for construction briefing, which is critical to the successful delivery of construction projects. Three techniques, i.e., function analysis system technique, shared space, and computer-aided toolkit, are incorporated into the proposed approach. A computer-aided toolkit is developed to facilitate the implementation of FPS in the briefing processes. This approach can facilitate systematic, efficient identification, clarification, and representation of client requirements in trail running. The limitations of the approach and future research work are also discussed at the end of the paper.

关键词： briefing value management (VM) functional performance specification (FPS) function analysis system technique shared space computer-aided

Alkylimido osmium(VI) and ruthenium(VI) porphyrins. Crystal and molecular structures of Os(TTP)(O)(NBu^t)·EtOH and Os(4-Cl-TPP)(NBu^t)_2

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Chinese Journal of Chemistry 1997年第1期15卷 1-12页

作者： LI, Zao-YingDepartment of Chemistry, Wuhan University, Wuhan, Hubei 430072, ChinaCHE, Chi-Ming Department of Chemistry, The hong kong University, hong kong Department of Chemistry Wuhan University Wuhan Hubei 430072 China Department of Chemistry Hong Kong University Hong Kong Hong Kong

Oxo(tert-butylimido) or bis(tert-butylimido)osmium(VI) porphyrins Os(Por)(O)(NBut) and Os(Por)(NBut)2, [Por=meso-tetrakis(p-tolyl)porphyrinato (TTP) and meso-tetrakis(4-chlorophe-nyl)porphyrinato (4-Cl-TPP)] were synthesized by air oxidation of bis(tert-butylamme)osmium(II) porphyrins [Os(Por)(H2NBut)2 (Por=TPP, 4-Cl-TPP], depending on whether tert-butylamine is present. The bis(tert-butylamine)ruthenium(II) porphyrins [Ru(Por)(H2NBut)2, Por=TTP, 4-Cl-TPP] can undergo bromine oxidation to give oxo(tert-butylimido)ruthenium(VI) complexes in quantitative yields. All these new complexes were characterized by 1H NMR, UV-Visible and IR spectroscopy. The X-ray crystal structures of Os(TTP)(O)(NBut).EtOH and Os(4-Cl-TPP)(NBut)2 have been determined. Crystal data: for Os(TTP)(O)(NBut).EtOH: monoclinic, space group P21/c, a=1.3546(6) nm, b=2.3180(3) nm, c=1.6817(3) nm, B=90.84(2), V=527.97(1) nm3, Z=4. The Os=O and Os=NBut distances in Os(TTP)(O)(NBut).EtOH are 0.1772(7) nm and 0.1759(9) nm, respectively. The average Os=NBut distance of Os(4-Cl-TPP)(NBut)2 is 0.1775 nm.

关键词： Alkylimido ruthenium osmium porphyrin crystal structure

A theoretical analysis on efficiency of some Newton-PCG methods

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Science China Mathematics 2005年第8期48卷 1046-1064页

作者： DENG Naiyang, ZHANG Jianzhong & Zhong Ping China Agricultural University, Beijing 100083, China City University of hong kong, hong kong, China China Agricultural University Beijing China City University of hong kong Hong Kong China

In this paper, we study the efficiency issue of inexact Newton-type methods for smooth unconstrained optimization problems under standard assumptions from theoretical point of view by discussing a concrete Newton-PCG algorithm. In order to compare the algorithm with Newton's method, a ratio between the measures of their approximate efficiencies is investigated. Under mild conditions, it is shown that first, this ratio is larger than 1, which implies that the Newton-PCG algorithm is more efficient than Newton's method, and second, this ratio increases when the dimension n of the problem increases and tends to infinity at least at a rate In n/In 2 when n→∞, which implies that in theory the Newton-PCG algorithm is much more efficient for middle- and large-scale problems. These theoretical results are also supported by our preliminary numerical experiments.

关键词： unconstrained optimization, Newton's method, preconditioned conjugate gradient method, efficiency.

Finite Volume Methods for Solving Electromagnetic Problems in Nonhomogeneous Media

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Wuhan University Journal of Natural Sciences 2003年第S1期8卷 176-182页

作者： Zou JunDepartment of Mathematics. The Chinese University of hong kong, Shatin, N. T. , hong kong, China. Department of Mathematics The Chinese University of Hong Kong Shatin N. T Hong Kong China

This article shall review some of the recent advances on finite volume methods for solving electromagnetic problems in nonhomogeneous media. The stability, convergence and applications of the finite volume methods wil... 详细信息

关键词： Maxwell equations finite volume methods stability convergence

Digital Rights Management for a Chinese XML Text Centre

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现代图书情报技术 2002年第S1期 172-177页

作者： Wai -man Wong(The Open University of hong kong Library,hong kong,China) TheOpenUniversityofhongkongLibraryhongkongChina

The Electronic Text Centre of the OpenUniversity of hong kong(OUHK)has been in full operationsince early *** currently houses 7,300+electronictexts,including free electronic titles,electronic titlespurchased directly from the market,and about,1,000 locallyproduced electronic *** locally produced titles are notavailable in the market but require local digitization andnegotiation with publishers with regard to the right to use(RTU)them so as to meet the learning needs of the ***...

关键词： Text Digital Rights Management for a Chinese XML Text Centre XML