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Expression and characterization of a novel GH128 laminarinase OUC-BsLam26 harboring both hydrolyzing and transferring activities

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Food Science and Human Wellness 2024年

作者： Yueyang Dong Yongyi Quan hong jiang Hamed I.Hamouda Zewei Lu Yimiao Chen Chengqiang Li Xiangzhao Mao State Key Laboratory of Marine Food Processing and Safety Control， College of Food Science and Engineering， Ocean University of China Qingdao Key Laboratory of Food Biotechnology Sanya Ocean Research Institute， Ocean University of China Processes Design and Development Department， Egyptian Petroleum Research Institute Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Key Laboratory of Biological Processing of Aquatic Products，China National Light Industry

Laminarin oligosaccharides (LOSs) with a specific degree of polymerization prepared through the laminarin degradation via laminarinase present more significant nutritional functions and application values. Human intestinal bacteria are promising potential producers of novel carbohydrate-active enzymes with unique properties. Here， a novel glycoside hydrolase family 128 (GH128) laminarinase OUC-BsLam26 from the intestinal bacterium Bacteroides sp. CBA7301 was heterologously expressed and characterized. The recombinant OUC-BsLam26 with a molecular mass of 49.86 kDa exhibits highest activity (6.60 U/mg) at 45 ℃ and pH 6.0， which shows noticeable temperature and pH stability. The purified OUC-BsLam26 could degrade laminarin via an endo-type mode with the generation of laminaripentaose， laminaritetraose， laminaritriose， and laminaribiose， among them， laminaritetraose is the principal product， which accounts for 45.25% of the total products， which is significantly different from the reported GH128 laminarinases. The minimum recognition substrate of OUC-BsLam26 is laminarihexaose. Furthermore， OUC-BsLam26 also could catalyze the transglycosylation process with the production of some novel glycosides. Altogether， the intestinal bacterium Bacteroides sp. CBA7301 contains laminarinase with unique product composition and OUC-BsLam26 is a hopeful bio-catalyst with the potential to produce laminaritetraose and some novel glycosides.

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巩固中国式现代化的政治认同基础

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东南大学学报（哲学社会科学版） 2024年第3期26卷 5-13,F0002,150页

作者：蒋红范晓云南大学马克思主义学院云南昆明650500

政治认同是一种重要而复杂的政治现象。推进中国式现代化,需要以全体中国人民高度的政治认同为基础。巩固中国式现代化的政治认同基础,应着重从利益认同、制度认同、价值认同三个方面进一步明确中国式现代化政治认同的科学内涵和实践要... 详细信息

政治认同是一种重要而复杂的政治现象。推进中国式现代化,需要以全体中国人民高度的政治认同为基础。巩固中国式现代化的政治认同基础,应着重从利益认同、制度认同、价值认同三个方面进一步明确中国式现代化政治认同的科学内涵和实践要求;需要遵循理论逻辑与历史逻辑的辩证统一,系统梳理中国式现代化政治认同的形成与发展历程,从中把握中国式现代化政治认同的发展趋势与基本规律。当前,在“两个大局”深刻演变的大背景下,中国式现代化政治认同面临着一系列问题和挑战,加强中国式现代化政治认同的现实建构,要以“两个结合”为科学方法论指导,重点从加强思想引领、坚持党的领导、增强话语自信等三个方面采取有力举措。

关键词：中国式现代化政治认同 “两个结合”

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Transcatheter Aortic Valve Replacement in Patients With Pure Native Aortic Regurgitation:Results From a Multicenter Registry Study

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Cardiology Discovery 2024年第2期4卷 134-141页

作者： Xiaofei Gao Juan Zhang Xiangquan Kong Jing Chen Xiang Chen Longyan Zhang Xinyong Cai Jiancheng Zhui Nailiang Tian Zhen Ge Bin Wang Qing Zhou Xi Su Lang hong Yan Wang hong jiang Junjie Zhang Shaoliang Chen Department of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210000China Department of Cardiology Renmin Hospital of Wuhan UniversityWuhan UniversityWuhanHubei 430000China Department of Cardiology Xiamen Cardiovascular HospitalXiamen UniversityXiamenFujian 361000China Department of Cardiology Wuhan Asian Heart HospitalWuhanHubei 430000China Department of Cardiology Jiangxi Provincial People's HospitalNanchang UniversityJiangxi 330000China

Objective:Patients with untreated severe aortic regurgitation(AR)have a high risk of *** transcatheter aortic valve replacement(TF-TAVR)is a treatment option for AR;however,the safety and efficacy of this technique have not been sufficiently *** study aimed to evaluate the clinical and anatomical variables correlating with device success of TF-TAVR using a self-expanding valve system for pure ***:Patients with pure native severe AR who underwent TF-TAVR using a self-expanding valve system were registered at 5 Chinese *** primary endpoint was device success at 1 month after *** secondary endpoint was the composite of major adverse cardiovascular events(MACE)at 6 months,including all-cause death,ischemic stroke,emergency conversion to cardiac surgery,and permanent pacemaker *** was used to analyze the left ventricular function before the TAVR procedure and during *** logistic regression and Cox regression analyses were performed to find relevant independent risk ***:Between September 2019 and February 2022,79 patients with AR were enrolled in the *** 1 month,device success was achieved in 60(75.9%)*** 6 months,29(36.7%)patients had *** revealed improved left ventricular function after *** regression analysis demonstrated that the Society of Thoracic Surgeons risk score(odds ratio 0.760,95%confidence interval(Cl):0.584-0.989;P=0.041)and annulus perimeter(odds ratio 0.888,95%Cl:0.796-0.992;P=0.035)were 2 predictors of device ***,annulus perimeter(<80.2mm),but not Society of Thoracic Surgeons risk score,was associated with a significant reduction in MACE at 6 months(hazard ratio 2.223,95%Cl:1.060-4.659;P=0.028).Conclusions:TF-TAVR using a self-expanding valve system appears to be a safe and feasible treatment for patients with pure native severe AR,particularly those with a less enlarged annulus.

关键词： Aortic valve Aortic regurgitation Transcatheter aortic valve replacement Device success Self-expanding valve

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Association of TRMT2B gene variants with juvenile amyotrophic lateral sclerosis

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Frontiers of Medicine 2024年第1期18卷 68-80页

作者： Yanling Liu Xi He Yanchun Yuan Bin Li Zhen Liu Wanzhen Li Kaixuan Li Shuo Tan Quan Zhu Zhengyan Tang Feng Han Ziqiang Wu Lu Shen hong jiang Beisha Tang Jian Qiu Zhengmao Hu Junling Wang Department of Neurology Xiangya HospitalCentral South UniversityJiangxiNational Regional Center for Neurological DiseasesNanchang330038China Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease Department of UrologyXiangya HospitalCentral South UniversityChangsha410078China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310002China Department of Neurology Xiangya HospitalCentral South UniversityChangsha410078China National Clinical Research Center for Geriatric Diseases Xiangya HospitalCentral South UniversityChangsha410008China Key Laboratory of Hunan Province in Neurodegenerative Disorders Central South UniversityChangsha410008China Center for Medical Genetics School of Life SciencesCentral South UniversityChangsha410008China Engineering Research Center of Hunan Province in Cognitive Impairment Disorders Central South UniversityChangsha410078China Hunan International Scientific and Technological Cooperation Base of Neurodegenerative and Neurogenetic Diseases Changsha410078China Hunan Key Laboratory of Molecular Precision Medicine Xiangya HospitalCentral South UniversityChangsha410078China

Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons,and it demonstrates high clinical heterogeneity and complex genetic architecture.A variation within TRMT2B(c.1356G>T;p.K452N)was identified to be associated with ALS in a family comprising two patients with juvenile ALS(JALS).Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS,and three more variants were identified in a public ALS database including 3317 patients with ALS.A decreased number of mitochondria,swollen mitochondria,lower expression of ND1,decreased mitochondrial complex I activities,lower mitochondrial aerobic respiration,and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 ***,TRMT2B variations overexpression cells also displayed decreased *** conclusion,a novel JALS-associated gene called TRMT2B was identified,thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B amyotrophic lateral sclerosis mitochondrial complex I tRNA methylation reactive oxygen species

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ASF1A-dependent P300-mediated histone H3 lysine 18 lactylation promotes atherosclerosis by regulating EndMT

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Acta Pharmaceutica Sinica B 2024年第7期14卷 3027-3048页

作者： Mengdie Dong Yunjia Zhang Minghong Chen Yongkang Tan Jiao Min Xian He Fuhao Liu Jiaming Gu hong jiang Longbin Zheng Jiajing Chen Quanwen Yin Xuesong Li Xiang Chen Yongfeng Shao Yong Ji hongshan Chen Department of Cardiovascular Surgery the First Affiliated Hospital of Nanjing Medical Universityand Key Laboratory of Cardiovascular and Cerebrovascular MedicineSchool of PharmacyNational Vaccine Innovation PlatformNanjing Medical UniversityNanjing 211166China Key Laboratory of Cardiovascular and Cerebrovascular Medicine School of PharmacyNanjing Medical UniversityNanjing 211166China Department of Clinical Medicine Nanjing Medical University TIANYUAN Honors SchoolNanjing Medical UniversityNanjing 211166China Department of Anesthesiology Sir Run Run HospitalNanjing Medical UniversityNanjing 211166China Department of Cardiovascular Surgery the First Affiliated Hospital of Nanjing Medical UniversityNanjing 211166China Key Laboratory of Cardiovascular and Cerebrovascular Medicine Key Laboratory of Targeted Intervention of Cardiovascular DiseaseCollaborative Innovation Center for Cardiovascular Disease Translational MedicineState Key Laboratory of Reproductive MedicineSchool of Pharmacythe Affiliated Suzhou Hospital of Nanjing Medical UniversityGusu SchoolNanjing Medical UniversityNanjing 211166China National Key Laboratory of Frigid Zone Cardiovascular Diseases(NKLFZCD) Department of Pharmacology(State-Province Key Laboratories of Biomedicine-Pharmaceutics of China)College of PharmacyKey Laboratory of Cardiovascular Medicine Research and Key Laboratory of Myocardial IschemiaChinese Ministry of EducationNHC Key Laboratory of Cell Transplantationthe Central Laboratory of the First Affiliated HospitalHarbin Medical UniversityHarbin 150081China Key Laboratory of Targeted Intervention of Cardiovascular Disease Collaborative Innovation Center for Cardiovascular Disease Translational MedicineNanjing Medical UniversityNanjing 211166China Department of Cardiology Huai’an First People’s Hospital Affiliated with Nanjing Medical UniversityHuai’an 223399China

Endothelial-to-mesenchymal transition(EndMT)is a key driver of *** glycolysis is increased in the endothelium of atheroprone areas,accompanied by elevated lactate *** lactylation,mediated by lactate,can regulate gene expression and participate in disease ***,whether histone lactylation is involved in atherosclerosis remains ***,we report that lipid peroxidation could lead to EndMT-induced atherosclerosis by increasing lactatedependent histone H3 lysine 18 lactylation(H3K18la)in vitro and in vivo,as well as in atherosclerotic patients’***,the histone chaperone ASF1A was first identified as a cofactor of P300,which precisely regulated the enrichment of H3K18la at the promoter of SNAI1,thereby activating SNAI1 transcription and promoting *** found that deletion of ASF1A inhibited EndMT and improved endothelial *** analysis based on Apoe^(KO)Asf1a^(ECKO) mice in the atherosclerosis model confirmed the involvement of H3K18la in atherosclerosis and found that endotheliumspecific ASF1A deficiency inhibited EndMT and alleviated atherosclerosis *** of glycolysis by pharmacologic inhibition and advanced PROTAC attenuated H3K18la,SNAI1 transcription,and EndMT-induced *** study illustrates precise crosstalk between metabolism and epigenetics via H3K18la by the P300/ASF1A molecular complex during EndMT-induced atherogenesis,which provides emerging therapies for atherosclerosis.

关键词： Histone lactylation Atherosclerosis Endothelial-tomesenchymal transition ASF1A SNAI1 Endothelial dysfunction Lactate Epigenetic

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Biomechanical effects of posterior lumbar interbody fusion with vertical placement of pedicle screws compared to traditional placement

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World Journal of Clinical Cases 2024年第20期12卷 4108-4120页

作者： Ji-hong jiang Chang-Ming Zhao Jun Zhang Rong-Ming Xu Lei Chen Department of Orthopedic Surgery Zhejiang University Mingzhou HospitalNingbo 315000Zhejiang ProvinceChina

BACKGROUND The pedicle screw technique is widely employed for vertebral body fixation in the treatment of spinal ***,traditional screw placement methods require the dissection of paraspinal muscles and the insertion of pedicle screws at specific transverse section angles(TSA).Larger TSA angles require more force to pull the muscle tissue,which can increase the risk of surgical trauma and ischemic injury to the lumbar *** To study the feasibility of zero-degree TSA vertical pedicle screw technique in the lumbosacral *** Finite element models of vertebral bodies and pedicle screw-rod systems were established for the L4-S1 spinal segments.A standard axial load of 500 N and a rotational torque of 10 N/m were *** screw pull-out experiment was conducted to observe pedicle screw resistance to pull-out,maximum stress,load-displacement ratio,maximum stress in vertebral bodies,load-displacement ratio in vertebral bodies,and the stress distribution in pedicle screws and vertebral *** between the 0-degree and 17-degree TSA were *** At 0-degree TSA,the screw pull-out force decreased by 11.35%compared to that at 17-degree TSA(P<0.05).At 0-degree and 17-degree TSA,the stress range in the screw-rod system was 335.1-657.5 MPa and 242.8-648.5 MPa,separately,which were below the fracture threshold for the screw-rod system(924 MPa).At 0-degree and 17-degree TSA,the stress range in the vertebral bodies was 68.45-78.91 MPa and 39.08-72.73 MPa,separately,which were below the typical bone yield stress range for vertebral bodies(110-125 MPa).At 0-degree TSA,the load-displacement ratio for the vertebral bodies and pedicle screws was slightly lower compared to that at 17-degree TSA,indicating slightly lower stability(P<0.05).CONCLUSION The safety and stability of 0-degree TSA are slightly lower,but the risks of screw-rod system fracture,vertebral body fracture,and rupture are within acceptable limits.

关键词： Vertical pedicle screw Pedicle screw technique Transverse section angle Lumbosacral segment Finite element analysis

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时间反转OFDM系统中增强安全性能的功率分配与人工噪声设计

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电子学报 2024年第5期52卷 1570-1581页

作者：雷维嘉毕文佳雷宏江唐宏重庆邮电大学通信与信息工程学院重庆400065 重庆邮电大学移动通信技术重庆市重点实验室重庆400065

时间反转技术特有的时空聚焦特性使其具有抗窃听能力.本文对提高时间反转正交频分复用系统安全性能的优化方案进行研究.首先,通过应用时间反转预滤波的时空聚焦特性来提高合法接收端相对于窃听端的信号强度;然后,以最大化保密速率为目... 详细信息

时间反转技术特有的时空聚焦特性使其具有抗窃听能力.本文对提高时间反转正交频分复用系统安全性能的优化方案进行研究.首先,通过应用时间反转预滤波的时空聚焦特性来提高合法接收端相对于窃听端的信号强度;然后,以最大化保密速率为目标对子载波的功率分配进行优化.为进一步提升系统保密传输能力,利用循环前缀提供的自由度实现零空间人工噪声,在对窃听端形成有效干扰的同时,不对合法接收端造成影响.通过对子载波功率分配和人工噪声协方差阵进行联合优化,最大化系统的保密传输速率.仿真结果表明,所提优化方案能显著提高系统的保密速率.

关键词：物理层安全正交频分复用时间反转功率分配人工噪声

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心脏性猝死风险预测需要全方位评估

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中华心血管病杂志 2024年第7期52卷 739-744页

作者：张萍江洪马长生清华大学附属北京清华长庚医院心血管内科北京102218 武汉大学人民医院心内科自主神经调控湖北省重点实验室武汉430060 首都医科大学附属北京安贞医院心内科北京100029

心脏性猝死(SCD)导致全球每年约500万人死亡,是全球重大公共卫生难题。SCD具有突发性、院外心搏骤停存活率低等特点,对家庭及社会造成巨大心理和经济负担。近年来,我国为降低SCD的发生率已做出极大努力。未来需纳入从临床到遗传的多模... 详细信息

心脏性猝死(SCD)导致全球每年约500万人死亡,是全球重大公共卫生难题。SCD具有突发性、院外心搏骤停存活率低等特点,对家庭及社会造成巨大心理和经济负担。近年来,我国为降低SCD的发生率已做出极大努力。未来需纳入从临床到遗传的多模态系统进行SCD预测的全方位评估。

关键词：猝死心脏危险因素预测

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基于深度学习的微纳光纤自动制备系统

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物理学报 2024年第10期73卷 212-220页

作者：刘鸿江刘逸飞谷付星上海理工大学光电信息与计算机工程学院上海200093

在微纳光纤拉锥制备过程中,对直径的大范围、高精度和动态实时性测量是实现低损耗传输和色散调控的关键.针对现有传统制备方法直径调控范围小、操作复杂及耗时长等问题,本文基于深度学习神经网络算法实现了微纳光纤自动检测系统.利用计... 详细信息

在微纳光纤拉锥制备过程中,对直径的大范围、高精度和动态实时性测量是实现低损耗传输和色散调控的关键.针对现有传统制备方法直径调控范围小、操作复杂及耗时长等问题,本文基于深度学习神经网络算法实现了微纳光纤自动检测系统.利用计算机视觉中的图像分割方法,通过制作高质量多尺度微纳光纤数据集,使用基于小目标检测改进的YOLOv8-FD算法对微纳光纤直径进行自动检测.在数据集中获得了平均均值精度高达mAP^(IoU=50)=0.975和mAP^(IoU=50_95)=0.765的性能参数.实验结果表明,该系统可实现微纳光纤直径462 nm—125μm范围,误差2.95%以内的测量和自动化制备,并随着光纤直径增长,误差逐渐缩小,且该系统光学成像单个像素分辨率为65.97 nm,平均检测时间为9.6 ms.本文工作适用于对微纳光纤的高精度实时测量和自动精确制备,为低损耗传输和色散可调的微纳光纤器件发展提供新的思路.

关键词：微纳光纤直径测量深度学习图像分割

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金融监管与商业信用供给——基于资管新规的准自然实验

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中南财经政法大学学报 2024年第4期 56-69页

作者：洪金明袁一辰江红中国财政科学研究院研究生院北京100142 莱西姆大学克拉罗·雷托高等研究院马尼拉1002

本文以2015—2021年我国沪深A股非金融业上市公司为样本,运用广义双重差分模型实证考察了资管新规实施对商业信用供给的影响。研究结果显示,资管新规实施后,前期金融化水平越高的企业商业信用供给增加越多,经过一系列稳健性检验后实证... 详细信息

本文以2015—2021年我国沪深A股非金融业上市公司为样本,运用广义双重差分模型实证考察了资管新规实施对商业信用供给的影响。研究结果显示,资管新规实施后,前期金融化水平越高的企业商业信用供给增加越多,经过一系列稳健性检验后实证结果依然稳健。机制分析发现,资管新规的实施通过抑制企业金融化、降低经营风险和缓解融资约束提高企业商业信用供给。异质性分析表明,较高的市场地位削弱了资管新规实施对商业信用供给的影响,而较多的银行信用、较宽松的货币政策和较高的影子银行发展水平却能增强资管新规实施对商业信用供给的影响。进一步分析发现,资管新规实施主要促进了一年以内和对下游客户的商业信用供给;资管新规的实施通过促进商业信用供给助力企业实现主业发展。本文的研究表明实施资管新规能够推动实体经济的健康发展,推动供应链上下游企业协同发展,对金融更好服务于实体经济具有重要的政策意义。

关键词：金融监管资管新规商业信用供给影子银行企业金融化水平

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