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Investigation of HcAR2 antagonists as a potential strategy to modulate bovine leukocytes

Journal of Animal Science and Biotechnology 2024年第4期15卷 1567-1577页

作者： Laman K.Mamedova Kirby c.Krogstad Paiton o.Mc.onald Laxman Pokhrel Duy H.Hua Evan c.Titgemeyer Barry J.Bradford Department of Animal Science Michigan State UniversityEast LansingMichigan 48824USA Department of Animal Sciences and Industry Kansas State UniversityManhattanKS 66506USA comparative Medicine and Integrative Biology East LansingMI 48824USA Department of chemistry Kansas State UniversityManhattanKS 66506USA

Background Dairy cows experiencing ketosis after calving suffer greater disease incidence and are at greater risk of leaving the herd. In vitro administration of beta-hydroxybutyric acid(BHBA;the primary blood ketone) has inhibitory effects on the function of bovine leukocytes. BHBA is a ligand of HcAR2 and the activation of these receptors promotes an anti-inflammatory response which may be related with immunosuppression observed in transition dairy cattle. The objective of this study was to identify and test antagonists for HcAR2 in bovine immune cells cultured with *** We observed expression of HcAR2 at the protein level within lymphocytes, monocytes, and granulocytes. The proportion of cells expressing HcAR2 tended to be greater in mid-lactation compared to early lactation cows;the increase was a result of increased proportion of T and B cells expressing HcAR2. Stimulation of HcAR2 with niacin or BHBA promoted ca^(2+) mobilization in neutrophils and mononuclear cells. Mononuclear cells treated with BHBA had diminished intracellular ca^(2+) responses when HcAR2 was knocked down by si RNA silencing, indicating ca^(2+) mobilization was mediated by HcAR2 signaling. Two candidate antagonists for HcAR2, synthesized from niacin(NA-1 and NA-5), were tested;monocytes and neutrophils pre-treated with NA-1 and NA-5 had reduced ca^(2+) mobilization after incubation with BHBA. Furthermore, NA-5 but not NA-1 prevented BHBA-associated reductions in cyclic *** We demonstrated that HcAR2 is present on bovine leukocytes and has greater expression later in lactation. We confirmed that BHBA and niacin derived HcAR2 antagonists alter bovine leukocyte activity. our results demonstrate that both BHBA and niacin affect bovine leukocyte ca^(2+) mobilization in a HcAR2-dependent manner.

关键词： Flow cytometry GPR109A HM74A Ketosis

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A ratiometric fluorescent probe for hypoxanthine detection in aquatic products based on the enzyme mimics and fluorescence of cobalt-doped carbon nitride

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Food Science and Human Wellness 2024年第2期13卷 879-884页

作者： Xin Wang chengyi hong Zhengzhong Lin Zhiyong Huang college of ocean Food and Biological Engineering Jimei UniversityXiamen 361021China

A ratiometric fluorescent probe for hypoxanthine(Hx)detection was established based on the mimic enzyme and fluorescence characteristics of cobalt-doped graphite-phase carbon nitride(co doped g-c_(3)N_(4)).In addition to emitting strong fluorescence,the peroxidase activity of co doped g-c_(3)N_(4)can catalyze the reaction of o-phenylenediamine and H_(2)o_(2)to produce diallyl phthalate which can emit yellow fluorescence at 570 *** the decomposition of Hx by xanthine oxidase,Hx can be indirectly detected by the generating hydrogen peroxide based on the measurement of fluorescent ratio I(F_(570)/F_(370)).The linear range was 1.7-272.2 mg/kg(R^(2)=0.997),and the detection limit was 1.52 mg/kg(3σ/K,n=9).The established method was applied to Hx detection in bass,grass carp,and shrimp,and the data were verified by *** result shows that the established probe is sensitive,accurate,and reliable,and can be used for Hx detection in aquatic products.

关键词： co doped g-c_(3)N_(4) Ratiometric fluorescent probe Hypoxanthine Freshness Aquatic products

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Fungal residues were more sensitive to nitrogen addition than bacterial residues in a meadow grassland soil

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Soil Ecology Letters 2024年第3期6卷 61-70页

作者： Zhifu Pei Mei hong Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources Key Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous RegionInner Mongolia Agricultural UniversityHohhot 010018China

Nitrogen(N)addition can significantly affect the amount of soil carbon(c)pools through biological routes,and mic.obial residues are important components of soil carbon ***,it remains unclear how N addition affects the accumulation of soil mic.obial residues in meadow *** study analyzed the effects of N addition on mic.obial residues in a meadow grassland soil,and the key fac.ors affecting the accumulation of mic.obial residues under N addition were analyzed in combination with soil physicochemical properties and mic.obial community *** results showed that N addition significantly changed the structure of the mic.obial communities and the accumulation of mic.obial residues,mainly manifested by a significant decrease in fungal biomass and the fungal/bacterial ratio(F/B),but had no significant effect on bacterial or total mic.obial biomass(PLFAs).N addition significantly increased the accumulation of fungal residues(7.45%),but had no significant effect on the accumulation of bacterial residues or total amino sugar(TAS).We found that fungal residues were more affected by soil environmental fac.ors than bacterial *** results of the random forest analysis showed that bacterial biomass under N addition was the most important predic.or of soil bacterial residues,whereas total N(TN),pH and F/B were the most important predic.ors of soil fungal *** summary,our results indicate that fungal communities and residues accumulation play important roles in regulating the response of grassland soil c to N addition,further enhancing our understanding of the mechanisms of soil carbon pool to N addition.

关键词： nitrogen addition mic.obial residues mic.obial community composition soil organic carbon

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A robust viscoelastic surfactant tolerating 20% Hcl up to 150℃ for oil well stimulation

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Petroleum Science 2024年第3期21卷 1865-1874页

作者： Ji Wang Ning Qi hong-Yao Yin Yu-Jun Feng Polymer Research Institute State Key Laboratory of Polymer Materials EngineeringSichuan UniversityChengdu610065SichuanPR.China Tianfu Yongxing Laboratory New Theory and Technology of CO_(2) Capture Research CenterChengdu610065SichuanPR China Sc.ool of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongPR China

Viscoelastic surfactants(VES)are often used as viscous diverters in acidizing stimulation to prolong the acid consumption time and maximize zonal coverage of the acid for improving well ***,the ceiling temperature of commercial VES cannot exceed 120℃in practical use because of the poor thermal stability and fragile molecular structure,hindering their implementation in hightemperature oil reservoirs,i.e.,≥150℃.Here we synthesized a novel c22-tailed diamine,N-erucaminopropyl-N,N-dimethylamine(EDPA),and examined comparatively its rheological behavior,assemblies morphology and molecular stability in 20 wt%Hcl with a commercial VES,erucyl dimethyl amidopropyl betaine(EDAB).The feasibility of EDPA for acidizing stimulation was assessed by acid etching of carbonate rock with its Hcl solution at 150℃.Rheological results showed that the 2.5 wt%EDPA—20 wt%Hcl solution maintains stable viscosity of 90 m Pa s at 150℃for 60 min,while that of 2.0 wt%EDAB Hcl solution is just 1 m Pa s under identical conditions.1H NMR spectra and cryo-TEM observations revealed that the chemical structure and self-assembled architectures of EDPA remained intact in such context,but the EDAB suffered from degradation due to the hydrolysis of the amide group,ac.ounting for the poor heat-resistance and *** reaction rate of 2.5 wt%EDPA Hcl solution with carbonate rock was one order of magnitude lower than that of 20 wt%Hcl solution at 150℃,underpinning the potential of EDPA to be used in the high-temperature reservoirs *** work improved the thermal tolerance of VES in highly concentrated Hcl solution,paving a feasible way for the acidization of high-temperature reservoir environments(~150℃).

关键词： Viscoelastic surfactants Thermal stability Self-diverting acid Wormlike micelles Acidizing stimulation

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Situational Analysis of Malaria Incidence Under Integrated Malaria Service in Hotspot Township-Minbya Township,Rakhine State,Myanmar,2017–2020

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china cDc weekly 2024年第17期6卷 374-377页

作者： Aung Than oo hong chen ASEAN centre for Biodiversity Ringgold standard institution D.M.Lantican AvenueUniversity of the Philippines Los Baños LagunaLos BañosPhilippines center for Global Public Health Chinese Center for Disease Control and PreventionBeijingChina

Introduction:Malaria is a significant public health concern and tropical disease,particularly affecting Myanmar within the Greater Mekong *** annual parasite index(API)exceeds 1 per 10,000 population in the northern seven townships of Rakhine State,with Minbya Township designated as a highburden area for malaria by the World Health organization(WHo)Myanmar and the National Malaria control Programme(NMcP).Since 2017,the Malaria reduction intensification plan has been in place in this township to combat the high disease transmission *** study aims to assess the malaria epidemiology in Minbya Township from 2017 to 2020 under the intensification plan for elimination,as well as to evaluate the effectiveness of the integrated strategy in reducing cases in hotspot ***:The study utilized a surveillance study design to collect secondary data from the Malaria surveillance system(MSS)and the epidemiologic monitoring dashboard of Minbya Township,Maruk-U District,located in Rakhine ***:Since 2017,the Malaria prevention and control(P&c)Program in Minbya Township has successfully decreased malaria morbidity,eliminated malaria-related deaths,and bolstered malaria testing capabilities through the participation of village health volunteers(VHVs).Approximately 87%of malaria prevention and control services are executed by the township’s malaria elimination and disease control programs,with additional support from *** API dropped from 13 in 2017 to 2.5 in 2020,with Plasmodium vivax being the most prevalent malaria species,ac.ounting for 55%of ***:The study suggests that early diagnosis and promotion of artemisinin-based combination treatment(AcT),along with strategic planning including expanding active case detection in rural health centers and implementing a communitybasedcommunitybased integrated healthcare approach,are effective and efficient strategies for malaria elimination.

关键词： Malaria prevention epidemiology

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coFe_(2)o_(4) decorated graphene/c_(18)-functionalized mesoporous silica nanocomposites prepared for magnetic enrichment and electrochemical detection of promethazine in beef

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chinese chemical Letters 2024年第3期35卷 208-213页

作者： Xingdong Yang Shi-Ming Ying Sen Zhang Jinxia Dai Wei Gao Tian-Qi Wang Jun-Qin Qiao hong-Zhen Lian Li Mao State Key Laboratory of Analytical chemistry for Life Science School of Chemistry&Chemical Engineering and Centre of Materials AnalysisNanjing UniversityNanjing 210023China Ministry of Education(MoE)Key Laboratory of Modern Toxicology School of Public HealthNanjing Medical UniversityNanjing 211166China

Promethazine(PHZ)is used as a sedative in veterinary medicine,and its residue can threaten the health of *** electrochemical detection of PHZ is suitable method for application in the ***,the traditional electroanalysis is difficult to perform directly in meat samples due to matrix *** work integrates magnetic solid-phase extraction and differential pulse voltammetry for highly sensitive and selective determination of PHZ in beef and beef liver for the first ***_(2)o_(4)/graphene coated with c_(18)-functionalized mesoporous silica(MG@mSio_(2)-c_(18))is synthesized as dispersed magnetic adsorbent to extract *** glassy carbon electrode modified with nitrogen-doped hollow carbon mic.ospheres(HcM)attracts the MG@mSio_(2)-c_(18)with PHZ,and directly detects the PHZ without elution ***@mSio_(2)-c_(18)can separate PHZ to avoid the interference of impurities on following detection,and also concentrate PHZ on magnetic ***,the electrode modification with HcM can amplify the electrochemical signal of ***,the integrated PHZ determination method exhibits a wide linear range from 0.08μmol/L to 300μmol/L with a low limit of detection of 9.8 nmol/*** beef sample analysis presents excellent recovery,demonstrating that this protocol is promising for the rapid and onsite detection of PHZ in real meat samples。

关键词： Promethazine(PHZ) Drug residue Magnetic solid phase extraction Electrochemical detection Beef

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RDH12-associated retinal degeneration caused by a homozygous pathogenic variant of 146c>T and literature review

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International Journal of ophthalmology(English edition) 2024年第2期17卷 311-316页

作者： Jin Li Yi-Qun Hu hong-Bo cheng Ting Wang Long-Hao Kuang Tao Huang Xiao-He Yan Shenzhen Eye Hospital Jinan UniversityShenzhen Eye InstituteShenzhen 518040Guangdong ProvinceChina

AIM:To describe the clinical,electrophysiological,and genetic features of an unusual case with an RDH12 homozygous pathogenic variant and reviewed the characteristics of the patients reported with the same ***:The patient underwent a complete ophthalmologic examination including best-corrected visual acuity,anterior segment and dilated fundus,visual field,spectral-domain optical coherence tomography(ocT)and electroretinogram(ERG).The retinal disease panel genes were sequenced through chip capture high-throughput sequencing and Sanger sequencing was used to confirm the *** we reviewed the characteristics of the patients reported with the same ***:A 30-year male presented with severe early retinal degeneration who complained night blindness,decreased visual acuity,vitreous floaters and amaurosis *** best corrected vision was 0.04 oD and 0.12 oS,*** fundus photo and ocT showed bilateral macular atrophy but larger areas of macular atrophy in the left *** shows bilateral symmetrical *** revealed that the amplitudes of a-and b-wave were severely *** ERG showed decreased amplitudes in the local macular area.A homozygous missense variant c.146c>T(chr14:68191267)was *** clinical characteristics of a total of 13 patients reported with the same pathologic variant ***:An unusual patient with a homozygous pathogenic variant in the c.146c>T of RDH12 which causes late-onset and asymmetric retinal degeneration are *** clinical manifestations of the patient with multimodal retinal imaging and functional examinations have enriched our understanding of this disease.

关键词： RDH12 gene inherited retinal degeneration homozygous pathogenic variant clinical feature multi-mode imaging

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Interfacial reaction between AZ91D magnesium alloy melt and mild steel under high temperature

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china Foundry 2024年第2期21卷 159-167页

作者： Jia-hong Dai Jian-yue Zhang Bin Jiang Xiang-jun Xu Zhong-tao Jiang hong-mei Xie Qing-shan Yang Guo-qing Zhang college of Materials Science and Engineering Yangtze Normal UniversityChongqing 408100China National Engineering Research center for Magnesium Alloys College of Materials Science and EngineeringChongqing UniversityChongqing 400044China chongqing Jiulongyuan High-tech Industry Group co. Ltd.Chongqing 400080China Loncin Industry co. Ltd.Chongqing 400080China Department of Materials Science and Engineering The Ohio State UniversityColumbusOH 43210USA chongqing Key Laboratory of Materials Surface&Interface Science Chongqing University of Arts and SciencesChongqing 402160China Sc.ool of Metallurgy and Material Engineering Chongqing University of Science and TechnologyChongqing 401331China

The metallurgical quality control of magnesium(Mg)and Mg alloys in melting process is required to ensure a satisfied mechanical and corrosion performance,while the typical used steel crucible introduces impurities and interfacial interaction during melting ***,a systematic study about impurities diffusion and interfacial interaction between molten Mg and steel is *** the present study,the interfacial reaction between molten AZ91D Mg alloy and mild steel during melting process was investigated with the melting temperatures of 700℃,750℃ and 800℃.The results show that Al(Fe,Mn)intermetallic layer is the intermetallic primarily formed at the interfaces of AZ91D melt and mild ***,Al_(8)(Mn,Fe)5is indexed between Al(Fe,Mn)and ***_(3)c appears between the mild steel and Al(Fe,Mn)at 700℃ and 750℃,but absent at 800℃ due to the increased solubility of carbon in Mg *** is found that the growth of the intermetallic layer is controlled by diffusion mechanism,and Al and Mn are the dominant diffusing species in the whole interfacial reaction *** measuring the thickness of different layers,the growth constant was *** increases from 1.89(±0.03)×10^(-12)m^(2)·s^(-1)at 700℃ to 3.05(±0.05)×10^(-12)m^(2)·s^(-1)at 750℃,and 5.18(±0.05)×10^(-12)m^(2)·s^(-1)at 800℃.Meanwhile,the content of Fe is linearly increased in AZ91D with the increase of holding time at 700℃ and 750℃,while it shows a significantly increment after holding for 8 h at 800℃,indicating holding temperature is more crucial to determine the Fe content of AZ91D than holding time.

关键词： AZ91D mild steel interface reaction intermetallic growth kinetics

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Hyperglycemia activates FGFR1 via TLR4/c-Src pathway to induce inflammatory cardiomyopathy in diabetes

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1693-1710页

作者： Xiong chen Jinfu Qian Shiqi Liang Jianchang Qian Wu Luo Yujuan Shi hong Zhu Xiang Hu Gaojun Wu Xiaokun Li Guang Liang Department of Endocrinology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China chemical Biology Research center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China Department of Wound Repair the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Department of cardiology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Sc.ool of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 311399China

Protein tyrosine kinases (RTKs) modulate a wide range of pathophysiological events in several non-malignant disorders, including diabetic complications. To find new targets driving the development of diabetic cardiomyopathy (DcM), we profiled an RTKs phosphorylation array in diabetic mouse hearts and identified increased phosphorylated fibroblast growth fac.or receptor 1 (p-FGFR1) levels in cardiomyocytes, indicating that FGFR1 may contribute to the pathogenesis of DcM. Using primary cardiomyocytes and H9c2 cell lines, we discovered that high-concentration glucose (HG) transactivates FGFR1 kinase domain through toll-like receptor 4 (TLR4) and c-Src, independent of FGF ligands. Knocking down the levels of either TLR4 or c-Src prevents HG-activated FGFR1 in cardiomyocytes. RNA-sequencing analysis indicates that the elevated FGFR1 activity induces pro-inflammatory responses via MAPKs–NFκB signaling pathway in HG-challenged cardiomyocytes, which further results in fibrosis and hypertrophy. We then generated cardiomyocyte-specific FGFR1 knoc.out mice and showed that a lack of FGFR1 in cardiomyocytes prevents diabetes-induced cardiac inflammation and preserves cardiac function in mice. Pharmacological inhibition of FGFR1 by a selective inhibitor, AZD4547, also prevents cardiac inflammation, fibrosis, and dysfunction in both type 1 and type 2 diabetic mice. These studies have identified FGFR1 as a new player in driving DcM and support further testing of FGFR1 inhibitors for possible cardioprotective benefits.

关键词： Diabetic cardiomyopathy Protein tyrosine kinases FGFR1 cardiomyocytes Inflammatory responses Toll-like receptor4 c-Src NFkB

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Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

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World Journal of Gastroenterology 2024年第11期30卷 1588-1608页

作者： Xing-Nian Zhou Quan Zhang hong Peng Yu-Jie Qin Yu-hong Liu Lu Wang Ming-Liang cheng Xin-Hua Luo hong Li Department of Infectious Diseases The Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Department of Infectious Diseases Guizhou Provincial People's HospitalGuiyang 550001Guizhou ProvinceChina

BAcKGRoUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and *** silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and *** To investigate the association between ferroptosis and pyroptosis and the upstream regulatory *** This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.c57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galac.osamine(D-GalN)to induce *** D(GSDMD)^(-/-)mice were used as an experimental *** changes in liver tissue were monitored by hematoxylin and eosin ***,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial ***-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain ***1,p53,and GSDMD were assessed by immunofluorescence *** Serum AST and ALT levels were elevated in patients with ***1,solute carrier family 7a member 11(SLc7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-coA synthetase long-chain family member 4(AcSL4)protein levels were elevated in human ALF liver *** the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour nec.osis fac.or alpha,IL-6,IL-2 and c-c motif ligand 2 levels were decreased and hepatic impairment was *** mice with GSDMD knoc.out,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLc7A11,elevation of AcSL4,and iron accumulation)were *** vitro,knockdown of p53 and overexpression of GPX

关键词： Silent information regulator sirtuin 1 Ferroptosis Pyroptosis p53/glutathione peroxidase 4/gasdermin D Acute liver failure

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