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Acta Pharmacologica Sinica 2004年第12期25卷 81-85页

作者： hong xie, ke-ying chen, pei-hong zhu2.unit of cell signal transduction, key laboratory of neuroscience, shanghai institutes for biologicalsciences, chinese academy ofsciences, shanghai2.0031, china unit of cell signal transduction Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

AIM: To explore whether the differential effects of Zn2+ on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn2+. METHODS: [3H]ryanodine binding assays were performed to examine the effect of Zn2+ on ryanodine binding to the SR in the presence of pyrithione sodium (PyNa), a specific Zn2+ ionophore. RESULTS: As a control, PyNa up to 50 μmol/L did not induce any effect on ryanodine binding to the SR of cardiac muscle. But PyNa 1-100 μmol/L increased ryanodine binding in skeletal muscle with maximum binding (222.2 %±20.9 % of the control) and inhibited ryanodine binding to 50 % of the control at about 500 μmol/L. In the presence of PyNa 10 and 50 μmol/L the dose-dependence of the effect of Zn2+ in cardiac muscle was still monophasic and not changed by PyNa, while the biphasic effect of Zn2+ in skeletal muscle became monophasic. CONCLUSION: Different permeability of the SR to Zn2+ may account for the differential effects of Zn2+on ryanodine binding in skeletal and cardiac muscles. PyNa is not a strictly specific Zn2+ ionophore.

关键词： zinc ion pyrithione radioligand binding ryanodine receptor skeletal muscle myocardium

Effect of osmotic stress on spontaneous calcium sparks in rat ventricular myocytes

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Acta Pharmacologica Sinica 2006年第7期27卷 877-887页

作者： hong xie pei-hong zhu unit of cell signal transduction,shanghai institutes for Biological Sciences,chinese academy of Sciences,shanghai 2.0031,china unitofcellsignaltransduction ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China

Aim:To study whether the volume of cardiomyocytes and their functions wouldchange under severe pathological conditions or osmotic *** clarify the roleof ryanodine receptors/calcium release channels(RyRs)in the functional change,the effect of osmotic stress on spontaneous Ca2+sparks in rat ventricular myocyteswas ***:A laser scanning confocal microscope was used todetect spontaneous Ca2+sparks of intact or saponin permeabilized myocytes loadedwith *** and low tonicity was obtained by adding sucrose and reducingNaCl concentration in the external medium,***:In intactmyocytes the frequency of Ca2+sparks was increased and decreased byhyperosmotic(1.5 T)and hyposmotic(0.6 T)exposure,*** addition,hyperosmotic exposure increased the temporal parameters and decreased the spa-tial parameter of Ca2+sparks,while opposite changes occurred with *** spatio-temporal properties of Ca2+sparks were slightly affected byaltering[K~+]i(50-200 mmol/L)in saponin permeabilized myocytes in the presenceof 8% *** was observed that the spatio-temporal parameters of the Ca2+sparks in permeabilized myocytes were dose-dependently altered by *** velocity of Ca2+waves in intact and permeabilized myocyte was alsoaffected by osmotic pressure or ***:The effect of osmotic stresson the frequency of spontaneous Ca2+sparks might be ascribed to the change ofmyoplasmic Ca2+and Ca2+content in the sarcoplasmic reticulum,while the effecton the spatio-temporal properties is caused by the alteration of Ca2+diffusionmainly resulting from the morphological change of the myocytes.

关键词： osmotic stress calcium sparks rat ventricular myocytes

The structure of TRAF7 coiled-coil trimer provides insight into its function in zebrafish embryonic development

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Journal of Molecular cell Biology 2024年第1期16卷 43-53页

作者： Xiaozhen Song Ruixing Hu Yi chen Man Xiao hong Zhang Shengnan Wu Qing Lu Molecular Diagnostic laboratory Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200040China Bio-X institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education)Shanghai Jiao Tong UniversityShanghai 200030China laboratory of Development and Diseases and State key laboratory for Medical Genomics Shanghai Institute of HematologyRuĳin HospitalShanghai JiaoTong University School of MedicineShanghai 200025China

TRAF7 serves as a crucial intracellular adaptor and E3 ubiquitin ligase involved in signal transduction pathways,contributing to immune responses,tumor progression,and embryonic *** mutations within the coiled-coil(CC)domain and WD40 repeat domain of TRAF7 could cause brain tumors,while germline pathogenic mutations contribute to severe developmental ***,the precise molecular mechanism underlying TRAF7 involvement in embryonic development remains *** this study,we employed zebrafish as an in vivo model ***7 knock down caused defects in zebrafish embryonic *** determined the crystal structure of TRAF7 CC domain at 3.3Åresolution and found that the CC region trimerization was essential for TRAF7 functionality during zebrafish embryonic ***,disease-causing mutations in TRAF7 CC region could impair the trimer formation,consequently impacting early embryonic development of ***,our study sheds light on the molecular mechanism of TRAF7 CC trimer formation and its pivotal role in embryonic development.

关键词： TRAF7 coiled-coil domain crystal structure trimerization embryonic development

Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

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cell Research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI hong XU, hong WEI XUE, National laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, shanghai institutes for Biological Science (SIBS), The chinese academy of Sciences Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on "Plant Molecular Physiology and signal transduction", 300 Fenglin Road, 200032 shanghai, china 1National laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China 1National laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China 1National laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3'-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562 bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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cell Research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi hong XU, hong Wei XUE National laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, shanghai institutes for Biological Sciences (SIBS), chinese academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on "Plant Molecular Physiology and signal transduction", 300 Fenglin Road, 200032 shanghai, china Xue HW Chinese Acad Sci Shanghai Inst Biol Sci Inst Plant Physiol & Ecol Natl Lab Plant Mol Genet 300 Fenglin Rd Shanghai 200032 Peoples R China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

A novel alternative for pyrogen detection based on a transgenic cell line

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signal transduction and Targeted Therapy 2024年第3期9卷 1221-1229页

作者： Qing He Chuan-Fei Yu Gang Wu Kai-Qin Wang Yong-Bo Ni Xiao Guo Zhi-Hao Fu Lan Wang De-Jiang Tan Hua Gao Can Wang Gang chen Xu-hong chen Bo chen Jun-Zhi Wang National institutes for Food and Drug Control BeijingChina School of Life Science and Biopharmaceutics Shenyang Pharmaceutical UniversityShenyangChina shanghai Institute for Food and Drug Control ShanghaiChina keyMed Biosciences Inc. ChengduChina

Pyrogen,often as a contaminant,is a key indicator affecting the safety of almost all parenteral drugs(including biologicals,chemicals,traditional chinese medicines and medical devices).It has become a goal to completely replace the in vivo rabbit pyrogen test by using the in vitro pyrogen test based on the promoted‘reduction,replacement and refinement’principle,which has been highly considered by regulatory agencies from different *** used NF-κB,a central signalling molecule mediating inflammatory responses,as a pyrogenic marker and the monocyte line THP-1 transfected with a luciferase reporter gene regulated by NF-κB as an in vitro model to detect pyrogens by measuring the intensity of a fluorescence ***,we show that this test can quantitatively and sensitively detect endotoxin(lipopolysaccharide from different strains)and nonendotoxin(lipoteichoic acid,zymosan,peptidoglycan,lectin and glucan),has good stability in terms of NF-κB activity and cell phenotypes at 39 cell passages and can be applied to detect pyrogens in biologicals(group A&C meningococcal polysaccharide vaccine;basiliximab;rabies vaccine(Vero cells)for human use,freeze-dried;Japanese encephalitis vaccine(Vero cells),inactivated;insulin aspart injection;human albumin;recombinant human erythropoietin injection(CHO cell)).The within-laboratory reproducibility of the test in three independent laboratories was 85%,80%and 80%and the interlaboratory reproducibility among laboratories was 83.3%,95.6%and 86.7%.The sensitivity(true positive rate)and specificity(true negative rate)of the test were 89.9%and 90.9%,*** summary,the test provides a novel alternative for pyrogen detection.

关键词： pyrogen rabbit replace

Polarized Regulation of Glycogen Synthase Kinase-3β is Important for Glioma Migration and Invasion

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Polarized Regulation of Glycogen Synthase Kinase-3β is Impo...

Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： ying Hou Yi-Zheng Wang (laboratory of Neural signal transduction,key laboratory of Neurobiology,Institute of neuroscience,shanghai institutes of Biological Sciences,shanghai 2.0031,china)

In this study,we investigated the role of the glycogen synthase kinase-β(GSK-3β)in glioma migration and *** wound healing assay,administration of lithium chloride(LiC1)and SB216763,inhibitors of GSK-3, slowed down t... 详细信息

关键词： GSK-3β glioma invasion

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cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, SHOU LING XU, WEI MIN NI, ZHAO QING CHU, ZHI hong XU, hong WEI XUENational laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, shanghai institutes for Biological Science (SIBS), chinese academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on "Plant Molecular Physiology and signal Transduc-tion", 300 Fenglin Road, 200032 shanghai, china National laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on Plant Molecular Physiology and signal transduction Shanghai China

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

MORN motifs in plant PIPKs are involved in the regulation of subcellular localization and phospholipid binding

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cell Research 2006年第5期16卷 466-478页

作者： Hui Ma~(1,*) ying Lou~1 Wen Hui Lin~1 hong Wei Xue~1 ~1National key laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,shanghai institutes for Biological Science(SIBS),chinese academy of Sciences and Parmer Group of Max-Planck-Institute of Molecular Plant Physiology(MPI-MP)on"Plant Molecular Physiology and signal transduction",300 Fenglin Road,200032 shanghai,china 1National key laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China 1National key laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China 1National key laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China 1National key laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on “Plant Molecular Physiology and signal transduction” 300 Fenglin Road 200032 Shanghai China

Multiple repeats of membrane occupation and recognition nexus （MORN） motifs were detected in plant phosphati-dylinositl monophosphate kinase （PIPK）,a key enzyme in PI-signaling *** analysis indicates that allthe MORN motifs （with varied numbers at ranges of 7-9）,which shared high homologies to those of animal ones,werelocated at N-terminus and sequentially arranged,except those of OsPIPK1 and AtPIPK7,in which the last MORN motifwas separated others by an～100 amino-acid"island"region,revealing the presence of two kinds of MORN arrangementsin plant *** employing a yeast-based SMET （sequence of membrane-targeting） system,the MORN motifswere shown being able to target the fusion proteins to cell plasma membrane,which were further confirmed by expressionof fused MORN-GFP *** detailed analysis via deletion studies indicated the MORN motifs in OsPIPK1,together with the 104 amino-acid"island"region are involved in the regulation of differential subcellular localization,*** membrane or nucleus,of the fused *** Western blot analysis of the recombinant MORN polypep-tide,expressed in Escherichia coli,showed that MORN motifs could strongly bind to PA and relatively slightly to PI4Pand PI（4,5）*** results provide informative hints on mechanisms of subcellular localization,as well as regulationof substrate binding,of plant PIPKs.

关键词： membrane targeting PIPK MORN motif subcellular localization

Proteomics of TGF-β1 induced concomitant apoptosis and EMT in AML12 cells

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Proteomics of TGF-β1 induced concomitant apoptosis and EMT ...