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Overexpression of GmBIN2,a soybean glycogen synthase kinase 3 gene,enhances tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots

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Journal of Integrative Agriculture 2018年第9期17卷 1959-1971页

作者： WANG Ling-shuang CHEN Qing-shan XIN Da-wei QI Zhao-ming ZHANG Chao LI Si-nan JIN Yang-mei LI Mo mei hong-yao SU Anoyu WU Xiao-xia College of Agriculture Northeast Agricultural University/Key Laboratory of Soybean BiologyMinistry of EducationHarbin 150030P.R.China College of Resources and Environment Northeast Agricultural UniversityHarbin 150030P.R.China

Glycogen synthase kinase 3 （GSK3） is a kind of sedne/threonine kinase widely found in eukaryotes. Many plant GSK3 kinases play important roles in regulating stress responses. This study investigated BRASSINOSTEROID-INSENSITIVE 2 （GmBIN2） gene, a member of the GSK3 protein kinase family in soybean and an orthologue of Arabidopsis BIN2/AtSK21. GmBIN2 expression was increased by salt and drought stresses, but was not significantly affected by the ABA treatment. To examine the function of GrnBIN2, transgenic Arabidopsis and transgenic soybean hairy roots were generated. Overexpression of GmBIN2 in Arabidopsis resulted in increased germination rate and root length compared with wild-type plants under salt and mannitol treatments. Overexpression of GmBIN2 increased cellular Ca2~ content and reduced Na~ content, enhancing salt tolerance in transgenic Arabidopsis plants. In the soybean hairy root assay, overexpression of GmBIN2 in transgenic roots also showed significantly higher relative root growth rate than the control when subjected to salt and mannitol treatments. Measurement of physiological indicators, including proline content, superoxide dismutase （SOD） activity, and relative electrical conductivity, supported this conclusion. Furthermore, we also found that GmBIN2 could up-regulate the expression of some stress-related genes in transgenic Arabidopsis and soybean hairy roots. Overall, these results indicated that GmBIN2 improved tolerance to salt and drought in transgenic Arabidopsis and soybean hairy roots.

关键词： GmBIN2 gene abiotic stress transgenic Arabidopsis soybean hairy roots

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基于离散模型的内超环面齿轮干涉检验方法

中国机械工程 2017年第15期28卷 1784-1789页

作者：洪玫姚立纲福州大学机械工程及自动化学院福州350116

针对内超环面齿轮齿面加工局部和全局干涉现象,提出一种干涉检验方法,有效地避免了加工干涉。通过干涉检验面簇的引入和干涉检验线簇的构造,将内超环面齿轮齿面和刀具扫掠体离散化。运用内超环面齿轮齿面和刀具扫掠体离散模型,通过截平... 详细信息

针对内超环面齿轮齿面加工局部和全局干涉现象,提出一种干涉检验方法,有效地避免了加工干涉。通过干涉检验面簇的引入和干涉检验线簇的构造,将内超环面齿轮齿面和刀具扫掠体离散化。运用内超环面齿轮齿面和刀具扫掠体离散模型,通过截平面处刀具中心的求解,确定内超环面齿轮齿面加工局部和全局干涉区域。通过实例分析进一步验证了内超环面齿轮齿面加工干涉检验方法的可行性。

关键词：超环面行星蜗杆传动内超环面齿轮复杂曲面干涉检验离散建模

Identification of QTLs Associated with Resistance to Pseudomonas syringae *** in Soybean(Glycine max(L.)Merr)

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Journal of Northeast Agricultural University(English Edition) 2021年第2期28卷 1-14页

作者： mei hong-yao Liu Yang Pan Xiao-cheng Su An-yu Wu Xiao-xia College of Agriculture Northeast Agricultural UniversityHarbin 150030China 65301 Farm and Sideline Bases of Army Wudalianchi 164100HeilongjiangChina College of Resources and Environmental Sciences Northeast Agricultural UniversityHarbin 150030China Institute of Green Food in Heilongjiang Harbin 150030China

Soybean bacterial spot disease caused by Pseudomonas syringae *** which is a bacterial disease seriously affects soybean *** soybean germplasms and recombinant inbred lines(RILs)population were used to identify the resistant trait after inoculated with ***(***001)in this ***-density genetic mapping was obtained by specific length amplified fragment sequencing(SLAF-seq)of 149 RILs population which was derived from the crossing between Charleston and *** results indicated that 10 germplasm resources had four resistant germplasms included highly resistant cultivar Charleston,four susceptible varieties included Dongnong594 and two moderately resistant *** quantitative trait locus(QTLs)were detected in RILs population by the composite interval mapping(CIM)method,and located on Linkage Group(LG)D1b(chromosome two),LG C2(chromosome six)and LG H(chromosome 12),*** scores ranged from 2.68 to 4.95 and the phenotypic variation percentage was from 6%to 11%.Six candidate genes were detected,according to the result of gene annotation *** of them had relationship with protein kinase activity,protein phosphorylation and leucine rich repeat(LRR)transmembrane protein,which had high expression after inoculated with *** by qRT-PCR.

关键词： soybean QTL mapping Pseudomonas syringae pv.Glycinea bacterial spot disease candidate gene

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和田地区双语教育现状及英语教育前景分析

民族教育研究 2016年第5期27卷 112-117页

作者：曹红梅姚春林中央民族大学预科教育学院北京100081 华北理工大学外国语学院河北唐山063009

本文采用问卷法和访谈法调查了和田地区的语言使用、语言态度及维汉双语教育现状,分析其英语教育发展前景。针对师资质量、语言环境、教材教法、教育政策执行等方面存在的问题提出相应建议,应分流和引入并举,提高师资质量;创造条件让越... 详细信息

本文采用问卷法和访谈法调查了和田地区的语言使用、语言态度及维汉双语教育现状,分析其英语教育发展前景。针对师资质量、语言环境、教材教法、教育政策执行等方面存在的问题提出相应建议,应分流和引入并举,提高师资质量;创造条件让越来越多的新疆学生有机会在内地接受优质教育;加快教材和教辅建设,帮助教师摸索出适合和田地区学生的教学方法。

关键词：和田双语教育英语教育现状对策

Recent progress in the applications of presynaptic dopaminergic positron emission tomography imaging in parkinsonism

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Neural Regeneration Research 2025年第1期20卷 93-106页

作者： Yujie Yang Xinyi Li Jiaying Lu Jingjie Ge Mingjia Chen Ruixin yao mei Tian Jian Wang Fengtao Liu Chuantao Zuo Key Laboratory of Arrhythmias Ministry of EducationDepartment of Medical GeneticsShanghai East HospitalSchool of MedicineTongji UniversityShanghaiChina Department of Neurology National Research Center for Aging and MedicineNational Center for Neurological Disordersand State Key Laboratory of Medical NeurobiologyHuashan HospitalFudan UniversityShanghaiChina Department of Nuclear Medicine&PET Center National Center for Neurological Disordersand National Clinical Research Center for Aging and MedicineHuashan HospitalFudan UniversityShanghaiChina International Human Phenome Institutes(Shanghai) ShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Nowadays,presynaptic dopaminergic positron emission tomography,which assesses deficiencies in dopamine synthesis,storage,and transport,is widely utilized for early diagnosis and differential diagnosis of *** review provides a comprehensive summary of the latest developments in the application of presynaptic dopaminergic positron emission tomography imaging in disorders that manifest *** conducted a thorough literature search using reputable databases such as PubMed and Web of *** criteria involved identifying peer-reviewed articles published within the last 5 years,with emphasis on their relevance to clinical *** findings from these studies highlight that presynaptic dopaminergic positron emission tomography has demonstrated potential not only in diagnosing and differentiating various Parkinsonian conditions but also in assessing disease severity and predicting ***,when employed in conjunction with other imaging modalities and advanced analytical methods,presynaptic dopaminergic positron emission tomography has been validated as a reliable in vivo *** validation extends to screening and exploring potential neuropathological mechanisms associated with dopaminergic *** summary,the insights gained from interpreting these studies are crucial for enhancing the effectiveness of preclinical investigations and clinical trials,ultimately advancing toward the goals of neuroregeneration in parkinsonian disorders.

关键词： aromatic amino acid decarboxylase brain imaging dopamine transporter Parkinson’s disease parkinsonism positron emission tomography presynaptic dopaminergic function vesicle monoamine transporter type 2

Drosophila models used to simulate human ATP1A1 gene mutations that cause Charcot-Marie-Tooth type 2 disease and refractory seizures

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Neural Regeneration Research 2025年第1期20卷 265-276页

作者： yao Yuan Lingqi Yu Xudong Zhuang Dongjing Wen Jin He Jingmei hong Jiayu Xie Shengan Ling Xiaoyue Du Wenfeng Chen Xinrui Wang Institute of Life Sciences College of Biological Science and EngineeringFuzhou UniversityFuzhouFujian ProvinceChina NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate(Fujian Maternity and Child Health Hospital) FuzhouFujian ProvinceChina Medical Research Center Fujian Maternity and Child Health HospitalCollege of Clinical Medicine for Obstetrics&Gynecology and PediatricsFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology and Institute of Neurology of The First Affiliated Hospital Institute of Neuroscienceand Fujian Key Laboratory of Molecular NeurologyFujian Medical UniversityFuzhouFujian ProvinceChina

Certain amino acids changes in the human Na^(+)/K^(+)-ATPase pump,ATPase Na^(+)/K^(+)transporting subunit alpha 1(ATP1A1),cause Charcot-Marie-Tooth disease type 2(CMT2)disease and refractory *** develop in vivo models to study the role of Na^(+)/K^(+)-ATPase in these diseases,we modified the Drosophila gene homolog,Atpα,to mimic the human ATP1A1 gene mutations that cause *** located within the helical linker region of human ATP1A1(I592T,A597T,P600T,and D601F)were simultaneously introduced into endogenous Drosophila Atpαby CRISPR/Cas9-mediated genome editing,generating the Atpα^(TTTF)*** addition,the same strategy was used to generate the corresponding single point mutations in flies(Atpα^(I571T),Atpα^(A576T),Atpα^(P579T),and Atpα^(D580F)).Moreover,a deletion mutation(Atpα^(mut))that causes premature termination of translation was generated as a positive *** these alleles,we found two that could be maintained as homozygotes(Atpα^(I571T)and Atpα^(P579T)).Three alleles(Atpα^(A576T),Atpα^(P579)and Atpα^(D580F))can form heterozygotes with the Atpαmut *** found that the Atpαallele carrying these CMT2-associated mutations showed differential phenotypes in *** heterozygous for Atpα^(TTTF)mutations have motor performance defects,a reduced lifespan,seizures,and an abnormal neuronal *** Drosophila models will provide a new platform for studying the function and regulation of the sodium-potassium pump.

关键词： ATP1A1 Atpα bang-sensitive paralysis Charcot-Marie-Tooth disease type 2 CRISPR/Cas9 homology-directed repair Na^(+)/K^(+)-ATPase point mutation seizures sodium pump

Palladium installed copper-organic framework for C–C coupling reactions

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Science China Chemistry 2025年

作者： Yu-mei Wang mei-Xia Tao Kai-Ming Mo Xu Chen Shu-Hua Zhong Elena S Shubina Guo-hong Ning Dan Li College of Chemistry and Materials Science Guangdong Provincial Key Laboratory of Supramolecular Coordination ChemistryJinan University A.N.Nesmeyanov Institute of Organoelement Compounds Russian Academy of Sciences

The formation of C–C bonds is a crucial aspect of organic synthesis. Synthesists' long-term objective is to identify novel catalysts to optimize reaction systems based on traditional transition metal catalysis. This study explores the synthesis and catalytic potential of palladium-installed covalent metal-organic frameworks(CMOFs) based on copper cyclic trinuclear units(Cu-CTUs). By incorporating palladium ions into the imine-based framework JNM-7 through a straightforward immersion method, we achieved a notable enhancement in catalytic activity for critical C–C coupling reactions, including C–H arylation and Sonogashira cross-coupling. The resulting JNM-7-Pd demonstrated remarkable stability and recyclability, maintaining its efficiency over at least three cycles without any decline in efficiency.

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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

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Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke yao Gaoying hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong Li Yuewei Chen Nian Liu Jing He Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

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空气质量如何影响中国城市房价?

城市与环境研究 2022年第3期 88-100页

作者：刘呈庆任玲梅洪尧山东师范大学经济学院 250014 上海财经大学公共经济与管理学院 200433 山东师范大学地理与环境学院 250014

近年来,城市的高房价和空气污染等问题成为社会广泛关注的焦点。本文基于2003—2018年中国269个城市PM浓度与新建商品房价格的面板数据,系统地考察了空气质量对房价的影响及其传导机制。实证结果表明,较差的空气质量整体上对城市房价有... 详细信息

近年来,城市的高房价和空气污染等问题成为社会广泛关注的焦点。本文基于2003—2018年中国269个城市PM浓度与新建商品房价格的面板数据,系统地考察了空气质量对房价的影响及其传导机制。实证结果表明,较差的空气质量整体上对城市房价有显著的负向影响,且对经济发展水平较高的大中城市的影响大于经济发展水平较为落后的小城市,空气质量可以通过人力资本机制、城镇化机制和房价预期机制来影响城市房价。城市的发展不能以牺牲环境质量为代价,经济发展水平较高的大中城市更应注重环境质量的改善。

关键词：空气质量房价异质性