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International Journal of Ophthalmology(English edition) 2016年第1期9卷 15-20页

作者： hong-hua Zheng Guo-Xing Xu Jian Guo Li-Cheng Fu Yao Yao Fujian Institute of Ophthalmology the First Affiliated Hospital of Fujian Medical University

AIM：To investigate the role of Aquaporin-1（AQP-1）in lens epithelial cells（LECs）and its potential target ***-1 is specifically expressed in LECs of eyes and is significant for lens homeostasis and transparency ***,AQP-1 expression in LECs was investigated to evaluate its influence on cell survival in association with its potential role in cataract formation.·M ETHODS：LECs were transfected with lentivirus carrying AQP-1 small interfering RNA（si RNA）.Real-time polymerase chain reaction（PCR）and Western blotting were conducted to detect AQP-1 expression in LECs from different ***,cell counting kit-8（CCK-8）assay and flow cytometry were performed to measure LEC proliferation and apoptosis,respectively.·RESULTS：AQP-1 expression was significantly reduced in LECs,both at m RNA and protein levels（〈0.05）,after si RNA *** cell viability was detected by CCK-8 assay in LECs with si RNA interference,compared to control cells（〈0.05）.The apoptosis rate significantly increased in cells after si RNA interference（〈0.05）.·CONCLUSION：The decreased cell viability following AQP-1 down regulation is largely due to its induction of apoptosis of ***-1 reduction might lead to changes of physiological functions in LECs,which might be associated with the occurrence and development of cataracts.

关键词： Aquaporin-1 small interfering RNA lensepithelial cells proliferation apoptosis cell counting kit-8 flow cytometry

The Influence of Transgenic cry1Ab/cry1Ac,cry1C and cry2A Rice on Non-Target Planthoppers and Their Main Predators Under Field Conditions

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Agricultural Sciences in China 2011年第11期10卷 1739-1747页

作者： HANYu XU Xue-liang MA Wei-hua YUAN Ben-qi WANG Hui LIU Fang-zhou WANG Man-qun WU Gang hua hong-xia Hubei lnsect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and Technology HuazhongAgricultural University Wuhan 430070 P.R.China

Transgenic Bt rice has been shown to be an effective means of controlling Lepidoptera pests of rice. However, the potential roles of transgenic rice on planthoppers and their predators need to be investigated before its commercialization. Population density, species dominance and population dynamics are important parameters of arthropods populations in field. So the impacts of three transgenic Bt rice strains expressing crylAb/crylAc, crylC and cry2A on population density, species dominance and population dynamics of three species of planthoppers （Nilaparvata lugens, Sogatella furcifera and Laodelphax striatellus） and their three main predators （ Cyrtorhinus lividipennis, Pirata subpiraticus and Theridium octomaculatum） were evaluated at three sites in Hubei Province, China, in the current study. The results showed that among three species of planthoppers, both in transgenic and non-transgenic rice field, the predominant species ofplanthoppers within phytophagous guild was S. furcifera at any site either growing season （46-50%）. Significantly higher population density ofN. lugens was observed in T2A-1 field relative to Minghui 63 field at Wuxue in 2010. The species dominance of predator, P. subpiraticus, in TT51 field was significantly higher than that in T 1 C-19 and T2A-1 fields in 2009 at Xiaogan site. Sampling date significantly influenced six arthropods except for P. subpiraticus in 2010. The interaction between rice strain^sampling date had no significant adverse effects on the population dynamics of three species of planthoppers and their predators, except for several individual species in 2009. The interaction among rice strain^sampling date^sampling site also had no significant effect on six arthropods except for S. furcifera in 2009. The results indicated that transgenic Bt rice expressing crylAb/crylAc, cry2A and crylC had no significant adverse effects on the population dynamics of three planthoppers and their predators in most investigated data and samp

关键词： planthopper predator transgenic Bt rice population dynamics species dominance population density

Effect of three insect-resistant maizes expressing Cry1le,Cry1Ab/Cry2Aj and Cry1Ab on the growth and development of armyworm Mythimna separata(Walker)

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Journal of Integrative Agriculture 2020年第7期19卷 1842-1849页

作者： SU hong-hua JIANG Tao SUN Yu GU Hui-jie WU Jiao-jiao YANG Yi-zhong School of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009P.R.China

Three transgenic maize events(IE09S034,Shuangkang 12--5 and C0030.3.5)produced Cry1le,Cry1Ab/Cry2Aj and G10-EPSPS,Cry1Ab and EPSPS,respectively,all of which target the Asian corn *** oriental armyworm Mythimna separata(Walker)is the secondary *** this study,the effects of the three Bt maizes on the development and survival of armyworm were *** results showed that IE09S034 had insecticidal activity against 1st instar larvae,and the survival rate of armyworm fed with Bt maize for 10 days was 462%,significantly lower than that of the *** larvae at 3rd--6th instar were more tolerant of the Bt toxin than the early instar ***,Shuangkang 12-5 had good insecticidal activity against 1st-5th instar *** mortality was nearly 100%when the larvae were fed with Shuangkang 12-5 before 3rd instar,and the toxin had quick acting *** event significantly ihibited the development of armyworm;that is,the larval duration of the 3rd and 4th instar larvae fed with Shuangkang 12-5 was prolonged by 4.5 and 3.0 days,*** pupal weight and egg number were also significantly lower than those of the *** C0030.3.5,it could control 1st--5th instar larvae *** mortality rates were all over 50%if 1st-3rd larvae were fed with this *** pupal weight of 4th-6th instar larvae fed with Bt maize were only 53.9,56.8 and 54.6%,respectively,compared to that of the *** number of eggs laid was significantly less than the *** results indicate that all three transgenic maize events exhibit the potential to provide effective control of early instar larvae of *** can be commercialized in future to control lepidoptera pests such as Asian corn borer and armyworm.

关键词： transgenic maize armyworm development survival

Effects of Variation in Activities of Starch-Sugar Metabolic Enzymes on Reducing Sugar Accumulation and Processing Quality of Potato Tubers

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Agricultural Sciences in China 2004年第7期3卷 519-527页

作者： CHENGShan-han SUZhen-hong XIECong-hua LIUJun NationalCentreforVegetableImprovement（CentralChinaBranch） KeyLaboratoryofHorticulturalPlantBiologyMinistryofEducation／HuazhongAgriculturalUniversityWuhan430070P.R.China

The experiment was designed, via storing potato tubers of cv. E-Potato1 and E-Potato3 indifferent temperatures, to explore the variation patterns of reducing sugar (RS) andtotal sugar (TS) contents and enzyme activities that are involved in the pathway ofstarch-sugar metabolism aiming at identifying the main factors that influence the chipcolor. The results showed that low temperature in storage was a main factor thataccelerated the accumulation of RS of the stored tubers and a very significant linearrelationship existed between RS content and chip color index (CCI) of the tubers. Furtheranalysis elucidated that when tubers stored at 4℃, the activities of ADP glucosepyrophosphorylase (AGPase), UDP glucose pyrophosphorylase (UGPase) and sucrose synthase(SuSy) were negatively exponential to the RS content significantly while that of acidinvertase and alkaline invertase was significantly linear to RS content. It suggestedthat these enzymes could play main roles in the cold sweetening of potato tubers throughregulating starch-sugar metabolism.

关键词： Potato RS TS CCI AGPase UGPase SuSy Invertase

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头戴式光场显示技术研究进展

光学学报 2023年第15期43卷 70-79页

作者：华宏 3D Visualization and Imaging Systems Laboratory Jame C.Wyant College of Optical SciencesThe University of ArizonaTucson 85721ArizonaUSA

光场显示器旨在通过重建三维场景在不同方向发出的几何光线来渲染三维场景的视觉感知,从而为人的视觉系统提供自然舒适的视觉体验,解决传统平面立体三维显示器中的聚散调节冲突问题。近年来,多种光场显示方法被尝试应用到头戴式显示技... 详细信息

光场显示器旨在通过重建三维场景在不同方向发出的几何光线来渲染三维场景的视觉感知,从而为人的视觉系统提供自然舒适的视觉体验,解决传统平面立体三维显示器中的聚散调节冲突问题。近年来,多种光场显示方法被尝试应用到头戴式显示技术中。本文对头戴式光场显示器的最新发展进行全面概述。

关键词：头戴显示器光场显示虚拟现实增强现实

NUMERICAL STUDY OF FLOW AND DILUTION BEHAVIOR OF RADIAL WALL JET

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Journal of Hydrodynamics 2010年第5期22卷 681-688页

作者： LI Zhi-wei huaI Wen-xin QIAN Zhong-dong ZENG Yu-hong YANG Zhong-hua State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan University Wuhan 430072 China

The radial wall jet is a flow configuration that combines the radial jet and the wall jet. This article presents a simulation of the radial wall jet by applying the transition Shear-Stress Transport （ SST） model. Tanaka’s experimental data are used for validation. The computed velocity profiles agree well with the experimental ones. The distributions of the velocity on cross-sections show a similarity in the main region and the profiles are different with those of the free radial jet or the wall jet, because the presence of the wall limits the expansion of the jet. By introducing the equivalent nozzle width, the maximum velocity decays and the half-width distributions are normalized, respectively. In addition to compare the flow field with experiments, this paper also analyzes the dilution effect of radial wall jets in terms of the concentration distributions. The concentrations on the wall keep constant within a certain distance from the nozzle. And the concentration distributions also show a similarity in the main region. Both the decays of the maximum concentration and the distributions of the concentration half-width fall into a single curve, respectively. The dilution effect of radial wall jets is thus verified.

关键词： radial wall jet velocity profile concentration distribution concentration half-width

Surface Plasmon Resonance and Raman Scattering Activity of the Au/AgxO/Ag Multilayer Film

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Chinese Physics Letters 2014年第4期31卷 147-149页

作者： Zhong Yu-Ting CHENG Zi-Qiang MA Liang WANG Jia-hong HAO Zhong-hua WANG Qu-Quan Key Laboratory of Artificial Micro- and Nano-structures of the Ministry of Education and School of Physics and Technology Wuhan University Wuhan 430072

The nanostructured Au/AgxO/Ag sandwich multilayer films on quartz substrates are prepared by the magnetron sputtering method. The morphology, plasmon resonance and surface enhanced Raman scattering （SERS） activi- ties of the multilayer films are studied. The resonant absorption wavelength of localized surface plasmon is tuned in a wide range from 618nm to 993nm by controlling the density of nanoparticles of Au and Ag. The SERS activity of the Au/AgxO/Ag multilayer films are enhanced over -10 times compared with those of bare Ag and bare Au films. These properties may find a potential application in biosensor and bioimaging.

关键词： SURFACE plasmon resonance RAMAN scattering SERS spectroscopy BIO-imaging sensors MAGNETRON sputtering

Biological activity of a red-tide alga—A. tamarense under co-cultured condition with bacteria

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Journal of Environmental Sciences 2005年第6期17卷 1047-1050页

作者： SU Jian-qiang YU Zhi-ming TIAN Yun SONG Xiu-xian hong hua-sheng ZHENG Tian-ling School of Life Sciences Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China Key Laboratory of Marine Ecology and Environmental Science ChineseAcademy of Sciences Qingdao 266071 China

The relationship between Alexandrium tamarense （Lebour） Balech, one of red-tide alga, and two strains of marine bacteria, Bacillius megaterium（S7 ） and B. halmapulus（ S10 ） isolated from Xiamen Western Sea, was investigated by evaluating the growth state of A. tamarense and the variation of β-glucosidase activity in co-culture system. The results showed the growth and multiplication of the alga were related with the concentration, genus speciality of the bacteria, and growth stage of the alga itself. The growth of A. tamarense was obviously inhibited by S7 and S10 at high concentration. Either inhibition or promotion contributed much more clearly in earlier than in later stageof the growth of the alga. Furthermore, there was a roughly similar variation trend of the activity of extra-cellular enzyme, β- glucosidase, in the water of the separately co-cultured bacteria S7 and S10 with the alga. The β-glucosidase activity（β-GlcA） rapidly increased during the later algal growth accompanying the increase of the lysis of the alga cells. The obvious inhibition of A. tamarense by marine bacteria at high concentration and evident increase of β-GlcA in co-colture system would help us in better understanding the relationship between red-tide alga and bacteria, and also enlightened us the possible use of bacteria in the bio-control of red-tide.

关键词： Alexandrium tamarense β-glucosidase bacteria

Risk Assessment of Nitrogen Discharge from a Coastal Watershed

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Pedosphere 2007年第4期17卷 467-474页

作者： CHEN Neng-Wang hong hua-Sheng ZHANG Luo-Ping CAO Wen-Zhi State Key Laboratory of Marine Environmental Science Environmental Science Research Center Xiamen University Xiamen 361005 （China）.

Probabilistic risk assessment （PRA） uses probability theory to quantify the probability of occurrence of an event. In this study, PRA was conducted for the discharge of excess nitrogen （N） from an agricultural （Jiulong River） watershed in Southeast China. Using fault tree analysis, a type of PRA, the probability of occurrence of excess N discharge into the river during a runoff event was evaluated both qualitatively and quantitatively. Land use maps, soil maps, fertilizer use records, and expert opinions were used to determine probabilities of individual events within the fault tree and to calculate the overall probability of excess N discharge during a runoff event. Qualitative analysis showed that the risk of excess N discharge was mainly related to crop and livestock practices in the watershed. Proper management of tillage, fertilizer, and manure was necessary to control N releases. Quantitative assessment results indicated that alternative practices including reduction of fertilization, installation of vegetative strip buffer around the pig farms, and installation of more riparian buffers along the Jiulong River could reduce the likelihood of N discharge through runoff.

关键词： N discharge probabilistic risk assessment runoff watershed

Synthesis and Herbicidal Activity of Novel Sulfonylureas Containing Thiadiazol Moiety

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Chemical Research in Chinese Universities 2008年第1期24卷 32-35页

作者： GUO Wan-cheng LIU Xing-hai LI Yong-hong WANG Su-hua LI Zheng-ming Institute of Elemento-Organic Chemistry State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071 P. R. China

Thirteen novel sulfonylureas containing thiadiazole moiety were synthesized in a two-step reaction. Their structures were determined using IR, ^1H NMR, HRFTMS, and elemental analysis. Herbicidal activities of these compounds were determined in the green house bio-assay. The results show that four compounds among them exhibit some activity toward four tested herbs.

关键词： Sulfonylurea Synthesis Herbicidal activity